scholarly journals DECOLOURIZATION OF TRIPHENYLMETHANE DYES AND DYE INDUSTRY EFFLUENT BY STAPHYLOCOCCUS AUREUS ISOLATED FROM DYE CONTAMINATED SITE

Author(s):  
R. Sandhiya ◽  
K. Sumaiya Begum ◽  
D. Charumathi

<p><strong>Objective: </strong>The objectives of the present study were a) to isolate and screen bacteria for dye removal from synthetic solution b) to optimize various variables such as pH, static/shaking and initial dye concentration on degradation of triphenyl methane dyes namely basic violet 3 and basic green 4 by isolated <em>Staphylococcus aureus</em> c) to analyse enzymes involved in the biodegradation of triphenylmethane dyes d) to treat real leather dyeing wastewater with newly isolated strain of <em>Staphylococcus aureus </em>e) to characterize untreated and treated leather dyeing wastewater f) to study the effects of real and treated effluent on plants and <em>Rhizobium</em>.<strong></strong></p><p><strong>Methods: </strong>Isolation of bacteria from sludge was carried out by spread plate method and the bacteria was identified by morphological and biochemical characterization. The isolated bacterium was screened for dye decolorization potential of triphenylmethane dyes basic violet 3 and basic green 4 The effects of parameters were studied by varying pH (from 3 to 9), temperature (from 15-45 °C), and initial dye concentration (from 10-500 mg/l). The enzyme involved in biodegradation was studied in intracellular extract. Real leather dyeing wastewater was treated with the bacteria and characterized. The treated wastewater was tested on plants and <em>Rhizobium </em>for toxicity. <strong></strong></p><p><strong>Results: </strong>Dye decolorization potential of bacteria <em>Staphylococcus aureus</em> isolated from wastewater for leather dyes basic violet 3 and basic green 4 were evaluated. Dye decolorization using bacteria was found to be dependent on physicochemical parameters (shaking, pH and initial dye concentration). Enzymes NADH-DCIP reductase and MG reductase were found to play dominant role during biodegradation of synthetic dyes. Application oriented studies using growing bacteria in pure cultures were carried out with leather dyeing wastewater collected from DKS prime tanners. Analysis of raw leather dyeing wastewater showed high pollution load in terms of color, Total solids, Total suspended solids, Total dissolved solids and Biological oxygen demand whereas the leather dyeing wastewater treated with pure culture of <em>Staphylococcus aureus</em> showed considerable decrease in Total solids, Total suspended solids, Total dissolved solids and Biological oxygen demand values which were within the permissible limits. Phytotoxicity and microbial toxicity studies confirmed the non-toxic nature of treated leather dyeing wastewater. <strong></strong></p><p><strong>Conclusion: </strong>Our study proved that <em>Staphylococcus aureus</em> can serve as a potential remediation agent for the treatment of leather dyeing wastewater.</p>

Author(s):  
Sipra Mallick ◽  
F Baliarsingh

Water quality index (WQI), a technique of rating water quality, is an effective tool to access quality and ensure sustainable safe use of water for drinking. The main objective of the present study is to access the surface water quality of Kathajodi river for knowing the suitability of drinking purpose by calculating the WQI. Samples were collected from selected locations during different seasons (winter, summer, rainy) over a period of 3 years (2011, 2012, 2013). Water quality assessment was carried out for the parameters like pH, total dissolved solids, total suspended solids, Alkalinity, Biological Oxygen Demand(BOD), Dissolved Oxygen(DO), Chloride, Nitrate, Alkalinity, Total Hardness, Calcium, Magnesium. The main objective is to develop a model to assess and predict the water quality changes of Kathajodi River Basin Odisha, India using neural networks and compared with the statistical methods. The result shows the proposed ANN prediction model has a great potential to simulate and predict the strongly correlated parameters like TSS (Total Suspended Solids), TDS(Total Dissolved Solids), Alkalinity, BOD(Biological Oxygen Demand)with Mean Square Error (MSE) : TSSMSE = 1.78 ; TDSMSE = 0; AlkalinityMSE = 3.77 and BODMSE = 8E-03.The Neural Network model has been compared with Linear Regression model to find out the best modelling approach for the study area. And it is concluded that the neural network model is superior to Linear Regression Model.


Wastewater is directly discharged into the ground or other water sources; it causes soil pollution and it affects the nature of the soil. Hence it is necessary to treat the wastewater before discharging. The objective is to treat the dairy wastewater using Bio Enzyme. Bio Enzyme is produced by the fermentation process of citric fruit peels, water, and jaggery. To reduce the time of the fermentation process, yeast is added into the Bio Enzyme. Then the parameters like Biological Oxygen Demand(BOD), Chemical Oxygen Demand(COD), Total Solids(TS), Total Dissolved Solids(TDS), Total Suspended Solids(TSS), pH, Alkalinity, Chlorides and Oil & Grease present in Dairy wastewater after the treatment using Bio Enzyme in different percentages (2%,4%&6%) were found. Then from the results the efficient percentage of Bio Enzyme for treating the Diary Wastewater was found.


2007 ◽  
Vol 2 (2) ◽  
pp. 107 ◽  
Author(s):  
Bakti Berlyanto Sedayu ◽  
Jamal Basmal ◽  
Diini Fithriani

Industri pengolahan alkali treated cottonii (ATC) menghasilkan limbah cair yang sangat besar. Pendaur ulangan limbah cair akan mengefisienkan penggunaan air untuk pengolahan sekaligus mengurangi masalah pencemaran lingkungan. Untuk itu dilakukan ujicoba proses daur ulang limbah cair ATC menggunakan alat yang dirancang oleh Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan yang berkapasitas 360 liter/jam. Pengolahan limbah cair dilakukan dengan teknik koagulasi dan filtrasi. Proses koagulasi dilakukan dengan penambahan bahan koagulan tawas (Al 2(SO4)3 ) dan flokulan blok di dalam tangki koagulasi, sedangkan filtrasi dilakukan dengan melewatkan limbah melalui kolom zeolit‑arang aktif dan filter selulosa asetat. Pengamatan dilakukan terhadap limbah cair dan air hasil olahan yang meliputi total padatan terlarut (Total Dissolved Solids/TDS), total padatan anorganik terlarut (Total Inorganic Dissolved Solids/TIDS), Biological Oxygen Demand (BOD), pH, kekeruhan, bau, dan warna limbah. Hasil penelitian menunjukkan bahwa pengolahan limbah cair ATC dapat menurunkan jumlah TDS, TIDS dan BOD hingga 56,60%, 32,29%, dan 60,66%, meskipun kemampuan alat filtrasi menggunakan kolom zeolit‑arang aktif menurun seiring dengan banyaknya proses daur ulang yang dilakukan. Berdasarkan hasil analisis didapatkan juga bahwa proses daur ulang limbah cair ATC mampu menurunkan nilai pH, kekeruhan, bau, dan warna limbah. Dengan pengolahan tersebut limbah cair dapat didaur ulang hingga 5 kali untuk proses pengolahan ATC berikutnya.


2015 ◽  
Vol 16 (SE) ◽  
pp. 395-403
Author(s):  
Abbas Ghaffari Habib ◽  
Seyed Hadi Khatami

In Bahar County (Iran), rivers are among the important sources of water for the agricultural sector. Therefore, this research evaluated the parameters of temperature, pH, Total Dissolved Solids (TDS), turbidity, nitrate, total phosphate, dissolved oxygen (DO), Biological Oxygen Demand (BOD5), and fecal coliform at five stations for five months (from February 2015 to June 2015) to determine water quality in the rivers. Based on this evaluation, the NSFWQI index was calculated and, finally, the routes of the rivers were zoned. The best water quality was recorded at Station Number 3 with the NSFWQI Value of 80 in January, and the worst at Station Number 5 (latgah) with the NSFWQI Value of 37 in June. Based on the mean NSFWQI indices, water quality was Medium at Stations Number 1, 2, and 4, good at Station Number 3, and bad at Station number 5.


2019 ◽  
Vol 6 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Veymar G. Tacias-Pascacio ◽  
Abumalé Cruz-Salomón ◽  
José H. Castañón-González ◽  
Beatriz Torrestiana-Sanchez

Background: Wet coffee processing consists of the removal of the pulp and mucilage of the coffee cherry. This process generates a large amount of acidic wastewater which is very aggressive to the environment because of its high content of recalcitrant organic matter. Therefore, treatment is necessary before discharge to water bodies. Because of this reason, this study aimed to evaluate the organic matter removal efficiency in an Anaerobic Baffled Bioreactor (ABR) coupled to a Microfiltration Membrane (MF) system as a new eco-friendly option in the treatment of wet Coffee Processing Wastewater (CPWW). Methods: Two systems (S1 and S2) were evaluated at Hydraulic Retention Times (HRT) of 59 h and 83 h, respectively. Both systems were operated at mesophilic conditions, at a Transmembrane Pressure (TMP) of 50 kPa during 1800 h. Results: The S2 generated higher organic matter removal efficiency, reaching removal values of turbidity of 98.7%, Chemical Oxygen Demand (COD) of 81%, Total Solids (TS) of 72.6%, Total Suspended Solids (TSS) of 100%, and Total Dissolved Solids (TDS) of 61%, compared with the S1. Conclusion: The S2 represents a new eco-friendly alternative to treat CPWW and reduce its pollutant effect.


2008 ◽  
Vol 273-276 ◽  
pp. 770-775 ◽  
Author(s):  
Teresa Castelo-Grande ◽  
Paulo A. Augusto ◽  
P. Monteiro ◽  
Domingos Barbosa

In this work the use of Membrane Bioreactors to treat leachate effluents is discussed. The problem of membrane fouling is addressed, and some of the efforts being done to overcome this problem presented. The process optimization requires the reduction of other parameters, some directly related to mass transfer, such as the total amount of solids, the total dissolved solids and the total suspended solids. The preliminary results obtained show the high potential of this technique for the treatment of leachates, mainly for the reduction of solids in leachate streams.


2017 ◽  
Vol 13 (3) ◽  
pp. 167
Author(s):  
Husnah Husnah ◽  
Eko Prianto ◽  
Siti Nurul Aida

Sungai Musi merupakan sungai besar mengaliri wilayah Sumatera Selatan, Lampung, dan Bengkulu , dan bervariasi dalam pemanfaatannya, khususnya di bagian hilir, didominasi oleh kegiatan industri yang membuang limbahnya ke Sungai Musi. Kajian pengaruh industri terhadap Sungai Musi telah dilakukan, namun sebatas analisis fisik dan kimia lingkungan dan belum mengarah kepada pengaruhnya terhadap organisme air. Organisme air adalah indikator penting perubahan lingkungan karena organisme khususnya organisme dasar (benthos) menyimpan sejarah proses-proses terjadi di perairan. Riset yang bertujuan untuk mengetahui kualitas perairan Sungai Musi bagian hilir ditinjau dari karakteristik fisik dan kimia dan struktur makrozoobenthos telah dilakukan di Sungai Musi, Sumatera Selatan pada bulan Mei dan September 2006. Riset dilakukan bersifat survei lapangan. Delapan stasiun ditentukan di Sungai Musi bagian hilir berdasarkan pada perbedaan mikrohabitat. Stasiun riset masing masing antara lain Sejagung, Pulokerto, Jembatan Ampera, Sebokor, Pulau Burung, Upang, Pulau Payung, dan Sungsang. Pada masing masing stasiun, dilakukan pengambilan contoh air untuk parameter fisika, kimia, dan makrobenthos. Contoh air diambil dari atas perahu motor pada kedalaman 1,0 m dari permukaan air dengan menggunakan kemmerer water sampler. Sebagian contoh dianalisis di lapangan (suhu, pH, dan oksigen terlarut) dan sebagian lagi yaitu jumlah padatan tersesuspensi (total suspended solids), jumlah padatan terlarut (total dissolved solids), jumlah karbon organik (total organic carbon), organik karbon terlarut (dissolved organic carbon), konsumsi oksigen biologi (biochemical oxygen demand), nitrat, dan fosfat dianalisis di laboratorium kimia. Contoh makrozoobenthos diambil pada 10 titik di masing-masing stasiun, dengan menggunakan ekman dredge dengan bukaan mulut 400 cm2. Contoh makrobenthos pada masingmasing titik tersebut disortir dengan menggunakan saringan dan kemudian digabungkan (dikomposit) dan diawetkan dengan formalin 10%. Data kualitas air dianalisis dengan principle component analysis dan kelimpahan makrozoobenthos dianalisis dengan analisis cluster. Kualitas perairan di Sungai Musi bagian hilir dikelompokkan atas 2 yang mengalami tekanan berat yaitu dari Sejagung sampai dengan Pulau Burung dan tekanan ringan yaitu dari Upang sampai dengan Muara Sungai Musi. Kelompok pertama dicirikan oleh nilai konsentrasi total dissolved solids, total organic carbon, dan dissolved organic carbon yang tinggi diiringi dengan kelimpahan makrozoobenhthos yang rendah serta didominasi oleh Tubifex sp. Kelompok ke-2 dari Upang sampai dengan Muara Sungai Musi dicirikan oleh nilai konsentrasi total suspended solids yang tinggi, dengan kelimpahan makrozoobenthos yang tinggi dan didominasi oleh Gammarus. Musi River is a large river , crossing three provinces, South Sumatra, Lampung and Bengkulu, and differeing in types and levels of its resources ultization, particularly at the down stream of Musi River, mostly dominated by industries activities producing a waste which flows to the river. Several studies on the effect of industries on the Musi River have been conducted , however , limmieted on physical dan chemical aspects of the water, not yet to evaluate its effect on aquatic organism. Aquatic organism such macrozoobenthos is important indicator of environmental changes since this organism records the history of processes occurred in the water. Study to assess water quality of the down stream Musi River based on physical, chemical water characteristics and macrozoobenthos community structure was conducted at may and september 2006 in Musi River located in South Sumatera Province of Indonesia. The study used inventory field survey. Eight sampling sites; Sejagung, Pulokerto, Jembatan Ampera, Sebokor, Pulau Burung, Upang, Pulau Payung, and Sungsang were selected based on the microhabitat difference. water sampling for physical and chemical parameters and sediment, and  macrozoobenthos were carried in each sampling site. Water sample was collected at a depth of 1.0 m from the water surface by using kemmerer water sampler. Some water quality parameters such as temperature, pH, and dissolved oxygen) were directly analyzed in the field, while the others such as total suspended solids, total dissolved solids, total organic carbon, dissolved organic carbon, biochemical oxygen demand, nitrate, and phosphate were analyzed in laboratory. Macrozoobenthos was collected at ten sampling points in each sampling sites using Ekman Dredge of 400 cm2 mouth opening. Macrozoobenthos from ten sampling points was composited, sorted and preserved with formalin 10%. Water quality parameters were analyzed with principle component analysis while macrozoobenthos abundance was analyzed with cluster. Results revealed that water quality at the down stream Musi River was classified into two groups. The first group was the heavy degraded sites from Sejagung to Pulau Burung, characterized by having high concentration of total dissolved solids, total organic carbon, and dissolved organic carbon, low abundance of macrozoobenthos with Tubifex sp. as the dominant species. The second group was light degraded sites from Upang to the mouth of Musi River, characterized by high concentration of total suspended solids and high macrozoobenthos abundance with Gammarus sp. as the dominant species.


2021 ◽  
Vol 49 (2) ◽  
pp. 257-265
Author(s):  
Jakia Hasan ◽  
Md Mozzammel Hoque ◽  
Ahmed Fazley Rabbi ◽  
Shafiqur Rahman ◽  
Md Zulfikar Ali

Potential spat collectors and suitable culture sites were investigated for the development of edible oyster culture from June to December 2019 at three study sites (Moheshkhali, the Jeti of Nuniarchora, and Sonadia) of Cox’s Bazar coast of Bangladesh. Four types of collectors such as oyster shell, earthen pot, kortal (windowpane oyster shell) and tiles were investigated. On site sampling of water quality parameters and number of spats on each collector were done at 15-days interval following standard methods. Results indicated insignificant difference (P > 0.05) in temperature, salinity, dissolved oxygen and pH, while significant difference (P < 0.05) in total dissolved solids, total suspended solids and Chl-a content of the water among the study locations. However, these parameters were in suitable range for growth and spawning of oyster. Results also indicated spat recruitment was significantly influenced by the types of collectors and study locations with significantly (P < 0.05) higher spat were recruited in Tiles. Tiles were also found to facilitate with an advantage of recycling of this collector. The location in interaction with polluting materials such as total dissolved solids and total suspended solids had a significant effect on spat recruitment rate and therefore, Sonadia was the most suitable location for spat recruitment. This study site can also be used as a suitable location for the development of oyster culture in the cost of Bay of Bengal, Bangladesh based on its environmental characteristics. Bangladesh J. Zool. 49 (2): 257-265, 2021


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