scholarly journals EVALUATION OF FREE RADICAL SCAVENGING ABILITY AND ANTIRADICAL ACTIVITIES OF XIMENIA CAFFRA FRUIT EXTRACTS AT DIFFERENT RIPENING STAGES

2019 ◽  
Vol 11 (1) ◽  
pp. 55
Author(s):  
Pensia E. Mapunda ◽  
Cosmas Mligo ◽  
Herbert V. M. Lyaruu
Keyword(s):  
Nitric Oxide ◽  
Free Radicals ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Human Beings ◽  
Ripening Stage ◽  
Fruit Extracts ◽  
Radical Scavenging Ability

Methods: Using standard procedures, Ximenia caffra fruit extracts were determined for ferrous ions chelating ability, nitric oxide and hydroxyl radical scavenging ability. Antiradical activities were assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) in which the fruit extracts were evaluated for the number of antiradical units (AU515), the number of antiradical units per 1 mg of extracts (EAU515) and the total number of antiradical units per 1g of raw fruits (TAU515).Results: The fruit extracts exhibited significant higher ferrous ion chelating and free radical scavenging capacity compared to synthetic antioxidants (standard). Fruit extracts in early ripening stage (ERS) exhibited stronger ion chelation, nitric oxide and hydroxyl radical scavenging ability with low effective fruit extract concentration required to reduce free radicals by 50% (EC50) that were 14, 25 and 30µg/ml respectively than the late ripening stage (LRS) extracts. The fruit extracts also showed high AU515, EAU515 and TAU515. The values of AU515 ranged from 0.93 to 0.95, while EAU515 from 78.30 to 79.34 and TAU515 ranged from 19762.46 to 23821.23 of the extracts in LRS and ERS respectively.Conclusion: Based on these observations Ximenia caffra is potentially beneficial to human health due to its strong ability to scavenge free radicals. Its utilization can potentially reduce the risk of degenerative diseases to human beings.

scholarly journals Free Radical Scavenging and Antioxidant Potential of Ethanolic Leaf Extract of Diodia sarmentosa on High Fat Fed Wistar Rats

2020 ◽  
pp. 54-63
Author(s):  
Ezejiofor Tobias I. Ndubuisi ◽  
Korie Stephen Chinedumije ◽  
Peter Chukwudi
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Free Radical ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
High Fat ◽  
Ethanolic Leaf Extract

Diodia sarmentosa (Sw) commonly known as Zimbabwe flora or Tropical button weed is a straggling or procumbent perennial herb which grows in evergreen forest, riverine vegetation and bush land. It has been used traditionally for the treatment of ulcer, diabetes and other ailments. This study investigated the Free radical scavenging and antioxidant potential of ethanolic leaf extract of Diodia sarmentosa on high fat fed wistar rats. Thirty (30) male wistar rats (150 g-200 g) were divided into five (5) groups: Negative control rats, Positive control rats, Low dose extract rats treated with 250 mg/kg, High dose extract rats treated with 500mg/kg of the extract, and Standard antihyperlipidaemic drug rats treated with 5 mg/kg of Simvastatin. High fat diet was fed to the rats with Ghee and Coconut oil in the ratio of 3:1 for six (6) weeks, and administration of the treatments started from the 3rd week till the 6th week. The free radical scavenging and antioxidant potentials of Diodia sarmentosa was determined by assessing parameters like nitric oxide radical, hydroxyl radical, malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) and Glutathione S-transferase (GST) in the serum of the test rats. Diodia sarmentosa scavenged free radicals In vitro by inhibiting Nitric oxide and Hydroxyl radicals in a concentration-dependent manner, showing an inhibitory concentration at 50% (IC50) of 907.17 µg/ml ± 45.36 and 2173.44 µg/ml ± 100.11 respectively. Results obtained showed antioxidant enzymes like SOD, GPx and CAT in the serum of the test rats were significantly increased (p<0.05) by both doses of the extract when compared to the PC group and was similar to the standard drug used. GST activity was decreased in the serum by both doses of the extract, this decrease was not significant (p>0.05), while Malondialdehyde level was significantly decreased (p<0.05) by both doses of the extract in the serum of the tests rats. Diodia sarmentosa has antioxidants potentials because of its ability to scavenge free radicals, replenish antioxidant enzymes and reduce lipid peroxidation.

scholarly journals Antioxidant Properties of the Leaves of Chromolaena odorata Linn

2013 ◽  
Vol 35 (1) ◽  
pp. 53-57
Author(s):  
D Bhargava ◽  
CK Mondal ◽  
JN Shivapuri ◽  
S Mondal ◽  
S Kar
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rational Approach ◽  
Chromolaena Odorata

 Introduction: Chromolaena odorata Linn (Asteraceae) locally known as “Banmara” has the reputation of being used as a medicinal herb in Nepal. A systemic search for drug research in now considered to be a rational approach. Therefore in the present study an effort has been made to evaluate the antioxidant activity of the leaves of the above mentioned plant. Methods: In the present research work the ethanolic and methanolic extract of leaves of the plant C. odorata Linn was assessed for free radical scavenging and antioxidant activity. In this work the ability of the extract to scavenge nitric oxide, hydroxyl radical and 1,1-diphenyl-2- picrylhydrazyl (DPPH) were used to assess its free radical scavenging potentials. Results: The DPPH radical inhibition (%) was 59.10, 52.13 and 81.12 for ethanolic extract, methanolic extract and ascorbic acid respectively. Similarly ethanolic and methanolic extract  also showed significant free radical scavenging action against nitric oxide and hydroxyl radical. Conclusion: Against the backdrop of many known medicinal properties of this plant its ethanolic and methanolic extract of leaves reveals significant antioxidant properties. DOI: http://dx.doi.org/10.2126/joim.v35i1.8900   Journal of Institute of Medicine, April, 2013; 35:53-57

Utilization Phase Transition Component Method to Prepare Specially Functionalized Nanoemulsion by Adding Resveratrol/Phenethyl Resorcinol Mixed Active Components and Application in Free Radicals Removal

2020 ◽  
Vol 20 (12) ◽  
pp. 7769-7774
Author(s):  
Wenwen Liu ◽  
Songzhu Cui ◽  
Jianhua Ji ◽  
Dejun Sun ◽  
Tao Wu
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Ionic Strength ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Mass Ratio ◽  
Hydrogen Binding ◽  
Active Components ◽  
Hydrophobic Binding ◽  
Radical Scavenging Ability

To explore the possibility of using a specially functionalized nanoemulsion for the removal of free radical, resveratrol and phenethyl resorcinol were employed to form resveratrol/phenethyl resorcinol mixed active components, and the removal efficiency of free radicals was evaluated. Original nanoemulsion and specially functionalized-nanoemulsion were characterized by DLS and EPR. The free radical scavenging ability of the mixed active components of resveratrol/phenethyl resorcinol was determined as a function of the mass ratio of resveratrol to phenethyl resorcinol, temperature, and ionic strength. In the results, the removal of free radicals by the resveratrol/phenethyl resorcinol nanoemulsion system was found to be more effective than the nanoemulsion alone. At a higher resveratrol ratio, more than 68% of free radicals could be removed. The efficiency was also found to increase with rising temperature. However, efficiency decreased with the increase in ionic strength. In conclusion, compared with conventional nanoemulsion, the combined utilization of nanoemulsion and the mixed active components of resveratrol/phenethyl resorcinol achieved better results for the removal of free radicals because of synergistic effect between nanoemulsion droplets and the mixed active components of resveratrol/phenethyl resorcinol, involving hydrophobic binding, hydrogen binding, and partitioning.

scholarly journals IN VITRO EVALUATION OF ENZYMIC ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITY IN THE ETHANOLIC EXTRACT OF CRESCENTIA CUJETE LEAVES

2020 ◽  
pp. 92-95
Author(s):  
ANITHA P ◽  
NAZEEMA TH
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Glutathione Reductase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Enzymic Antioxidants

Objectives: This study aims to evaluate the enzymic antioxidants and free radical scavenging present in the ethanolic leaf extracts of Crescentia cujete. Methods: Enzymic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase, glutathione peroxidase, and glutathione S-transferase (GST) were estimated by standard methods. Free radical scavenging potential was evaluated by diphenylpicrylhydrazyl (DPPH), nitric oxide, and hydroxyl radical methods using an ethanolic extract of C. cujete leaf. Results: The leaf extract of C. cujete showed the maximum activity of CAT, SOD, GST, glutathione reductase, and peroxidase activity. CAT activity was formed to be highest in the ethanolic extract of C. cujete leaf. DPPH radical scavenging activity was reported as 38.5 μg/ml, nitric oxide was found to be 200.77 μg/ml, and hydroxyl radical scavenging exhibited 108.42 μg/ml normalized with ascorbic acid. Conclusion: From the results, it has concluded that the ethanol extract of the C. cujete leaf has a prospective source of natural antioxidant that would be a great significance as therapeutic agents in preventing or slowing the progress of reactive oxygen species and related oxidative stress-related degenerative diseases.

scholarly journals FREE RADICAL SCAVENGING, ANTIOXIDANT POTENTIAL, AND NITRIC OXIDE INHIBITION IN HUMAN THP1 DERIVED MACROPHAGES BY KOKILAKSHAM KASHAYAM

2019 ◽  
pp. 82-86
Author(s):  
TEENA MERLIN ◽  
PRAKASH KUMAR B
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antioxidant Potential ◽  
Antioxidant Power ◽  
Chronic Inflammatory Condition ◽  
Herbal Decoction

Objective: The aim of this study was to evaluate the antioxidant potential of Kokilaksham kashayam and its effect on the production of nitric oxide (NO) by lipopolysaccharide (LPS) stimulated THP1 derived macrophages. Methods: Kokilaksham kashayam was subjected to fractionation and assessed for antioxidant activity. The effect of fractions on cell viability was determined using 3-(4,5 – dimethylthiazol -2-yl)-2,5-diphenyltetrazolium bromide assay and the fractions were evaluated for their effect on the production of NO by LPS stimulated THP1 derived macrophages. Results: It was found that the fractions of the herbal decoction were able to scavenge a variety of free radicals 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, NO, and hydroxyl radical. Ferric reducing antioxidant power assay showed the antioxidant capacity and cell culture studies in THP derived macrophages showed that the fractions inhibited the production of NO in LPS-stimulated THP1 derived macrophages. Conclusion: The overall study showed that the proinflammatory role of free radicals in general and specifically NO in chronic inflammatory condition could be managed by the use of Kokilaksham kashayam. The inhibitory effect of Kokilaksham kashayam on NO production and free-radical scavenging activity, in general, proves that the vital phytoconstituents in the herbal decoction are responsible for the antioxidant activity thereby preventing or slowing the process of chronic inflammatory conditions.

scholarly journals Assesment of free radical scavenging activity and inhibitory properties of aqueous extract of Cymodocea serrulata segrass towards α- glucosidase and α –amylase enzyme

2021 ◽  
Vol 23 (12) ◽  
pp. 339-360
Author(s):  
Suresh kumar Tirupur Venkatachalam ◽  
◽  
SenthilKumar Palanisamy ◽  
Sudhagar Babu ◽  
Ranjithkumar Rajamani ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Inhibitory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Cymodocea Serrulata

Aim: The study reports the in vitro free radical scavenging and α-amylase and α-glucosidase inhibition activities of aqueous extract of seagrass Cymodocea serrulata from Gulf of Mannar, Southeast Asia. Methods: The free radical scavenging activity was evaluated by DPPH, Superoxide, Nitric oxide and Hydroxyl radical scavenging assays and α-amylase, α-glucosidase enzyme inhibitory activity. The C. serrulata showed effective DPPH radical scavenging activity of 69.45 μg/mL, Superoxide radical scavenging activity of 91.08.5 ± 0.02%, Nitric oxide radical scavenging activity of 86.89 ± 0.03% and Hydroxyl radical scavenging activity of 81.09 ± 0.3 % at a concentration of 5 μg/mL respectively. It also showed significant inhibitory activity against α- amylase with an IC50 value of 3.0 ± 0.04 (μg/ml) and the α-glucosidase inhibitory activity with an IC50 value of 3.7 ± 1.01 (μg/ml) at concentrations of 1-5 μg/mL. Conclusion: Cymodocea serrulata was found to be a potent free radical scavenger and inhibitor of α-amylase and α-glucosidase enzymes.

scholarly journals Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy

2020 ◽  
Vol 8 (1) ◽  
pp. 75-81
Author(s):  
Bui Van Hoai ◽  
Ngo Dai Nghiep ◽  
Dao An Quang ◽  
Nguyen Thi Nam Phuong
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Free Radical ◽  
Trichoderma Viride ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Degree Of Deacetylation ◽  
Radical Scavenging Ability

Chitosan with 80% degree of deacetylation was hydrolyzed by cellulase of Trichoderma viride to prepare chitooligosaccharides (COSs) by the fractionation of the COSs with ultrafiltration membrane. The antioxidant activities of the COSs were clarified in this study by reducing power and free radical scavenging ability assay by UV-VIS absorption spectrum. The results show that the COS 1 (10,000-5,000 Da), COS 2 (5,000-3,000 Da), COS 3 (3,000-1,000 Da) and COS 4 (less than1,000 Da) segments have antioxidant properties.The antioxidant activitives of the COSs increased with the increment of concentration, and they also depended on molecular weight.

Sign in / Sign up

Export Citation Format

Share Document