Design and biofabrication of dermal regeneration scaffolds: role of oligomeric collagen fibril density and architecture

Aim: To evaluate dermal regeneration scaffolds custom-fabricated from fibril-forming oligomeric collagen where the total content and spatial gradient of collagen fibrils was specified. Materials & methods: Microstructural and mechanical features were verified by electron microscopy and tensile testing. The ability of dermal scaffolds to induce regeneration of rat full-thickness skin wounds was determined and compared with no fill control, autograft skin and a commercial collagen dressing. Results: Increasing fibril content of oligomer scaffolds inhibited wound contraction and decreased myofibroblast marker expression. Cellular and vascular infiltration of scaffolds over the 14-day period varied with the graded density and orientation of fibrils. Conclusion: Fibril content, spatial gradient and orientation are important collagen scaffold design considerations for promoting vascularization and dermal regeneration while reducing wound contraction.

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666 Autologous Skin Cell Therapy After Dermal Matrix with STSG in a 5-year-old African-American Female - How Adjunct Therapies are Refining the Dogmatic Treatment Approach to Burns

Journal of Burn Care & Research ◽

10.1093/jbcr/irab032.312 ◽

2021 ◽

Vol 42 (Supplement_1) ◽

pp. S189-S190

Author(s):

Alexandra Coward

Keyword(s):

African American ◽

Cell Suspension ◽

Healing Time ◽

African American Female ◽

Full Thickness ◽

Dermal Matrix ◽

Skin Cell ◽

Thickness Skin ◽

Dermal Regeneration

Abstract Introduction Split-thickness skin grafting (STSG) is the standard of care for the treatment of full thickness skin injuries. Skin grafts are associated with long-term morbidity including graft loss, adjacent structural injury, anesthetic complications, scarring, and scar contractures. Large surface area burns are additionally challenging due to limited donor site availability. Autologous skin cell suspension (ASCS) is a new adjunct for STSG using device that provides a suspension of non-cultured, autologous skin cells applied overtop of STSG. Dermal matrix templates are placed on wounds after burn excision and induces dermal regeneration in preparation for STSG, allowing for a thinner graft to be harvested and applied. This technique has been shown to require both smaller areas of donor skin as well as thinner skin harvest which improves both healing time and aesthetic outcomes of donor sites, enhancing the time-tested and well accepted technique of STSG. Methods We present the case of a 5-year-old African American female who suffered 18% TBSA deep partial thickness burns and full thickness burns to her abdomen, trunk and left back after her shirt was accidentally lit on fire at home. She was transferred from a local hospital to our burn center for further evaluation. She was evaluated by both the burn surgery and pediatric teams and admitted for wound cares and surgical planning. Results On hospital day five she underwent burn excision and placement of acellular dermal regeneration template. She returned to the operating room on hospital day 22 after daily wound cares for autografting with autologous skin cell suspension application to anterior and posterior torso and left arm, as well as to back and thigh donor sites. Her takedown on hospital day 29 showed excellent graft take. She was ultimately discharged on hospital day 47. She continued to undergo wound care in the outpatient burn clinic and daily physical and occupational therapy. Conclusions This case illustrates the use of dermal matrix and ASCS on a large burn with excellent aesthetic outcomes and improved healing time. This case is unique in highlighting the versatility of this therapy in a darker skinned patient. There are significant challenges with long term morbidity from STSG and the use of both dermal regenerative matrix and ASCS may provide surgeons with new approaches to decreasing depth and size of donor sites, as well as improving the length of hospital stay and overall aesthetic outcomes of donor and graft sites, specifically in darker skinned patients.

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Two native types of phytochrome A, phyAʹ and phyAʺ, differ by the state of phosphorylation at the N-terminus as revealed by fluorescence investigations of the Ser/Ala mutant of rice phyA expressed in transgenic Arabidopsis

Functional Plant Biology ◽

10.1071/fp16261 ◽

2018 ◽

Vol 45 (2) ◽

pp. 150 ◽

Cited By ~ 3

Author(s):

Vitaly A. Sineshchekov ◽

Larissa A. Koppel ◽

Cordelia Bolle

Keyword(s):

Transgenic Arabidopsis ◽

Emission Spectra ◽

Total Content ◽

The State ◽

Serine Phosphorylation ◽

Phytochrome A ◽

Wild Type ◽

Etiolated Seedlings ◽

Pre Treatment

Phytochrome A (phyA) mediates different photoresponses what may be connected with the existence of its two types, phyAʹ and phyAʹʹ, differing by spectroscopic, photochemical and functional properties. We investigated a role of phyA phosphorylation in their formation turning to transgenic Arabidopsis thaliana (L. Heynh.) phyA or phyAphyB mutants overexpressing rice wild-type phyA (phyA WT) or mutant phyA (phyA SA) with the first 10 serines substituted by alanines. This prevents phyA phosphorylation at these sites and modifies photoresponses. Etiolated seedlings were employed and phyA parameters were evaluated with the use of low temperature fluorescence spectroscopy and photochemistry. Germination of seeds was induced by white light (WL) pre-treatment for 15 min or 3 h. Emission spectra of rice phyA WT and phyA SA were similar and their total content was comparable. However, the phyAʹ/phyAʹʹ proportion in phyA WT was high and varied with the duration of the WL pre-treatment, whereas in phyA SA it was substantially shifted towards phyAʹʹ and did not depend on the pre-illumination. This suggests that phyA SA comprises primarily or exclusively the phyAʹʹ pool and supports the notion that the two phyA types differ by the state of serine phosphorylation. phyAʹʹ was also found to be much more effective in the germination induction than phyAʹ.

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Tensile Testing

10.31399/asm.tb.tt2.9781627083553 ◽

2004 ◽

Keyword(s):

Tensile Testing ◽

Uniaxial Tensile ◽

Uniaxial Tensile Test ◽

Bonded Joints ◽

Adhesively Bonded ◽

Adhesively Bonded Joints ◽

Design And Manufacturing ◽

Data Tables ◽

Reference Section

Tensile Testing, Second Edition is a comprehensive guide to the uniaxial tensile test and its use in determining the mechanical properties and behaviors of materials. The first six chapters cover the fundamentals of tensile testing, including the methodology, the equipment used, the effect of tensile loading on metals, the interpretation of data, and the role of tensile testing in design and manufacturing. The next six chapters deal with the testing of different classes of engineering materials, namely metals, plastics, elastomers, ceramics, and composites, and fabricated structures such as weldments and bolted and adhesively bonded joints. The book also includes three chapters on the nuances of tensile testing at extreme temperatures and strain rates and a reference section with data tables. For information on the print version, ISBN 978-0-87170-806-9, follow this link.

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Analisis Kritis Program Tayangan Asing di Televisi Swasta Indonesia

Jurnal Komunikasi Global ◽

10.24815/jkg.v10i1.20233 ◽

2021 ◽

Vol 10 (1) ◽

pp. 101-119

Author(s):

Sari Rahmani ◽

Irma Suryani

Keyword(s):

Critical Thinking ◽

Data Collection ◽

Total Content ◽

Qualitative Approach ◽

Television Programs ◽

Television Station ◽

Role Of The State ◽

The Media ◽

Study Case

Indonesia telah mengatur pembatasan tayangan asing di layar stasiun televisi dalam negeri. Regulasi tersebut tertuang dalam Undang-Undang Penyiaran Nomor 32 Tahun 2002 Pasal 36 ayat 2 yang mewajibkan setiap stasiun televisi untuk menayangkan sekurang-kurangnya 60% tayangan produksi domestik. Penelitian ini bertujuan untuk menganalisis permasalahan tayangan asing di televisi Indonesia dalam perspektif kritis industri budaya. Metode yang dilakukan adalah metode kualitatif studi kasus. Pengambilan data dari berbagai studi dokumen dan wawancara. Data yang telah dikumpulkan dianalisis dengan teknik pengumpulan data, reduksi data, penyajian data, dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa stasiun televisi swasta melanggar ketentuan mengenai muatan tayangan asing. Ada dua masalah utama tayangan asing di televisi Indonesia. Masalah pertama adalah lemahnya peran negara terhadap penyiaran di Indonesia. Kedua adalah masalah ekonomi media khususnya terkait dengan pekerja media. Indonesia issued the law regarding the limitation of foreign television programs in 2002. In Article 36, paragraph 2 of Law No. 32/2002 stated that every television station is obligated to broadcast domestic programs at least 60 percent of the total content. This research aimed to analyze foreign programs in Indonesia through a critical thinking perspective. This research adopted a study case, qualitative approach. Data collection was done through interviews, documents study. The collected data were analyzed by data collection, reduction, display, and conclusion drawing. The findings showed that The results showed that private television stations violated the provisions regarding the proportion of foreign programs. There were two main problems that emerge from foreign programs in private TV stations. The first problem was the weak role of the state in broadcasting in Indonesia. The second was the problem of the media economy, especially related to media workers.

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Climate Geoengineering and the Role of Public Deliberation: A Comment on the us National Academy of Sciences’ Recommendations on Public Participation

Climate Law ◽

10.1163/18786561-00504006 ◽

2015 ◽

Vol 5 (2-4) ◽

pp. 252-294 ◽

Cited By ~ 15

Author(s):

William C. G. Burns ◽

Jane A. Flegal

Keyword(s):

Public Participation ◽

Public Deliberation ◽

National Academy Of Sciences ◽

World Community ◽

Design Considerations ◽

The Us ◽

The World ◽

Academy Of Sciences ◽

Important Design

The feckless response of the world community to the mounting threat of climate change has led to a growing interest in climate geoengineering research. In early 2015, the us National Academy of Sciences released two major reports on the topic. While it is notable that both reports recommended some form of public participation to inform research, this article argues that the vagueness of these recommendations could mean that their implementation might not comport with optimal approaches for public deliberation. We outline some options for public deliberation on climate geoengineering and important design considerations.

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Role of Dermal Extract and Collagen Scaffold in Management of Post Burns Raw Area

New Indian Journal of Surgery ◽

10.21088/nijs.0976.4747.9218.24 ◽

2018 ◽

Vol 9 (2) ◽

pp. 240-242

Author(s):

Agrawal L ◽

◽

Sudhanva H.K. ◽

Preethitha B ◽

Kumaran M.S. ◽

...

Keyword(s):

Collagen Scaffold

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Designing a Computational Model of Learning

Machine Learning ◽

10.4018/978-1-60960-818-7.ch203 ◽

2012 ◽

pp. 147-174

Author(s):

David Gibson

Keyword(s):

Artificial Intelligence ◽

Computational Model ◽

Cognitive Models ◽

Design Framework ◽

Assessment Framework ◽

Design Considerations ◽

Learner Assessment ◽

How People Learn ◽

Evolutionary Perspectives

What would a game or simulation need to have in order to teach a teacher how people learn? This chapter uses a four-part framework of knowledge, learner, assessment, and community (Bransford et al., 2000) to discuss design considerations for building a computational model of learning. A teaching simulation—simSchool—helps illustrate selected psychological, physical, and cognitive models and how intelligence can be represented in software agents. The design discussion includes evolutionary perspectives on artificial intelligence and the role of the conceptual assessment framework (Mislevy et al., 2003) for automating feedback to the simulation user. The purpose of the chapter is to integrate a number of theories into a design framework for a computational model of learning.

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Discovery of increased epidermal DNAH10 expression after regeneration of dermis in a randomized with-in person trial — reflections on psoriatic inflammation

Scientific Reports ◽

10.1038/s41598-019-53874-z ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Heli Lagus ◽

Mariliis Klaas ◽

Susanna Juteau ◽

Outi Elomaa ◽

Juha Kere ◽

...

Keyword(s):

Protein Expression ◽

Psoriatic Skin ◽

Dermal Matrix ◽

Thickness Skin ◽

Dermal Regeneration ◽

Unaffected Skin ◽

One Year ◽

To Receive

AbstractBecause molecular memories of past inflammatory events can persist in epidermal cells, we evaluated the long-term epidermal protein expression landscapes after dermal regeneration and in psoriatic inflammation. We first characterized the effects of two dermal regeneration strategies on transplants of indicator split-thickness skin grafts (STSGs) in ten adult patients with deep burns covering more than 20% of their body surface area. After fascial excision, three adjacent areas within the wound were randomized to receive a permanent dermal matrix, a temporary granulation-tissue-inducing dressing or no dermal component as control. Control areas were covered with STSG immediately, and treated areas after two-weeks of dermis formation. Epidermis-dermis-targeted proteomics of one-year-follow-up samples were performed for protein expression profiling. Epidermal expression of axonemal dynein heavy chain 10 (DNAH10) was increased 20-fold in samples having had regenerating dermis vs control. Given the dermal inflammatory component found in our dermal regeneration samples as well as in early psoriatic lesions, we hypothesized that DNAH10 protein expression also would be affected in psoriatic skin samples. We discovered increased DNAH10 expression in inflammatory lesions when compared to unaffected skin. Our results associate DNAH10 expression with cell proliferation and inflammation as well as with the epidermal memory resulting from the previous regenerative signals of dermis. This study (ISRCTN14499986) was funded by the Finnish Ministry of Defense and by government subsidies for medical research.

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