scholarly journals Phytochemical screening of different extracts of Kalanchoe laciniata

2015 ◽  
Vol 1 (2) ◽  
pp. 58
Author(s):  
Maria Manan ◽  
Liaqat Hussain ◽  
Hira Ijaz ◽  
Bushra Nawaz ◽  
Muhammad Hanif
Keyword(s):  
Methanolic Extract ◽  
Hexane Extract ◽  
The Other ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Aqueous Methanol ◽  
Other Hand ◽  
Phytochemical Constituents

Alkaloids, tannins, saponins, steroids, terpenoids and flavonoids distribution in n-hexane and aqueous-methanolicextract of kalanchoelaciniata was assessed and compared. The present study was carried out to study the phytochemical constituents of Kalanchoe laciniata. Aqueous-methanol and n-hexane were the solvents used for the extraction of the plant. Phytochemical analysis was carried out on both of these extracts, indicated that n-hexane extract constitutes tannins, terpenoids on the other hand aqueous-methanolic extract contains saponins, tannins, terpenoids, flavonoids, glycosides  and anthraquinones. 

In-vitro anti-bacterial activity and phytochemical screening of crude extracts of Catharanthus roseus L. (G.) Don.

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Manish Kapoor ◽  
Jyoti Rani ◽  
Rupinder Kaur
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Mycobacterium Smegmatis ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Aqueous Methanol

Present study aims to investigate phytochemical analysis as well as in vitro antimicrobial activity of crude aqueous, methanol, ethanol, chloroform and petroleum ether extracts from leaves of C. roseus. Antimicrobial activity of extract was studied against various bacterial strains (Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Mycobacterium smegmatis). Qualitative preliminary phytochemical analysis revealed that alkaloids, phenols, flavonoids, amino acid and cardiac glycosides are present in the extracts. The result for total phenol and flavonoids content was the highest in methanol and the lowest in petroleum ether crude extract. The study revealed that inhibition significantly depend upon the solvent used for extraction and bacterial strain tested for susceptibility. Aqueous extracts were found less efficient as compared to organic solvent. Methanolic extract were found more effective against tested microbes.

FINGER PRINTING ANALYSIS OF THE PHENOLS FROM HOLOPTELEA INTEGRIFOLIA (ROXB.) PLANT LEAVES USING HPTLC

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (09) ◽  
pp. 67-71
Author(s):  
R. C. Sutar ◽  
◽  
D. S Musmade
Keyword(s):  
High Performance ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Plant Leaves ◽  
Methanol Extracts ◽  
Finger Printing ◽  
Rf Values ◽  
Layer Chromatography ◽  
Tungsten Lamp

The present study was conducted to identify the phenols from methanol extracts (MHI) of medicinally and economically useful leaves of Holoptelea integrifolia (Roxb.) plant using High Performance Thin Layer Chromatography (HPLC) technique. Preliminary phytochemical screening was done and HPTLC studies were carried out on CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp. Preliminary phytochemical screening of methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins, phenols and carbohydrates. HPT LC finger printing of phenols of methanolic extract of leaf revealed seven polyvalent phytoconstituents (7 peaks) and corresponding ascending order of Rf values in the range of 0.15 to 0.75. From the results of preliminary phytochemical analysis and above Rf values, we have concluded the presence of phenols in methanol extracts.

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

scholarly journals Quantitative and Qualitative Identification of Phytochemical Constituents of Sida Rhombifolia Leaves Extract

2019 ◽  
Vol 8 (2S4) ◽  
pp. 403-408
Keyword(s):  
Quantitative Analysis ◽  
Cardiac Glycosides ◽  
Total Content ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Biological Compounds ◽  
Potential Sources ◽  
Pharmaceutical Industries ◽  
Phytochemical Constituents ◽  
Sida Rhombifolia

Anti-inflammatory, analgesic, antimicrobial, anti-arthritic, antibacterial, antispasmodic, hypoglycaemic and hepatoprotective characteristics of Sida rhombifolia are included in the Malvaceae family. Most of the plant belongs to the Malvaceae family, as they comprise a number of phytochemicals and biological compounds, are potential sources of different medications. This is study on Sida rhombifolia leaf extract's tophytochemical constituen ts. Phytochemical screening results in herbal stan-dardizat ion and preparation and may relate the components to thei r medicinal / pharmacological uses. The qualitative phytochemical analysis has shown that the extract is positive for saponins, flavonoids, alkaloids, phenols and same extract is negative for carbohydrate, tannins, glycosides, cardiac glycosides, terpenoids, coumarins, steroid& phytosteroids, phlobutanins, anthraquinones. Quantitative analysis of phytochemicals includes the estimation of flavonoid, tannin and total content of phenol. The result suggest that the Sida rhombifolia leaves extract consist plenty of phytochemicals beneficial in alternative medical and pharmaceutical industries.

Pulicaria laciniata (Coss and Kral): Effects of Different Extraction Solvents on Phytochemical Screening and Antioxidant Activity

2021 ◽  
pp. 425-430
Author(s):  
Kamilia Bireche ◽  
Hocine Dendougui ◽  
Asma Abid ◽  
Abdeldjabbar Messaoudi ◽  
Mohamed Hadjadj
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
The Other ◽  
Phytochemical Screening ◽  
Butanol Extract ◽  
Metal Chelating ◽  
Phytochemical Investigation ◽  
Ic50 Value ◽  
Extraction Solvents ◽  
Phytochemical Constituents

This study aims to investigate phytochemical constituents of Pulicaria laciniata extracts and determine their antioxidant activity using three methods; Phosphomolybdate, Reducing Power, and Metal Chelating. The phytochemical investigation showed various secondary metabolites such as Phenols, Glycosides, Flavonoids, Alkaloids, Tannins, and Terpenoids. The N-butanol extract exhibited the highest antioxidant activity comparing with the other extract in all methods (0.51 and 0.65 mg/ml as A0.5 values of Phosphomolybdate, reducing power) and (1.65mg/ml for IC50 value of metal-chelating). In contrast, all the extracts showed week activity against the metal-chelating method.

Phytochemical Analysis and GC-MS Profiling in the Flower of Plumeria alba

2020 ◽  
Vol 981 ◽  
pp. 280-284
Author(s):  
Siti Aisyah Mohamad ◽  
Noor Suhana Adzahar ◽  
Muhammad Nadeem Akhtar ◽  
Seema Zareen ◽  
Thong Chuan Lee
Keyword(s):  
Chemical Constituents ◽  
Volatile Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Therapeutic Properties ◽  
Phytochemical Constituents ◽  
Screening Assays ◽  
Major Chemical ◽  
Ethylhexyl Phthalate

Therapeutic properties of the medicinal plant are due to the presence of phytochemical constituents. The phytochemical constituents of Plumeria alba flower were investigated by phytochemical screening assays and gas chromatography-mass spectrometry (GC-MS). The phytochemical screening of hexane, dichloromethane, ethyl acetate, butanol, and aqueous extracts of P. alba flower showed it contains a various concentration of saponins, flavonoids, tannins, steroids, volatile oil and phenolic compounds. Several major chemical constituents that were identified is squalene, bis(2-ethylhexyl) phthalate, methyl (methyl 4-O-methyl-α-d-mannopyranoside) uronate and tricyclo [7.2.0.0(2,6)] undecan-5-ol, 2,6,10,10-tetramethyl- (isomer 2) by using GC-MS technique.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF BAWANG DAYAK (ELEUTHERINE SP.) AND HATI TANAH (ANGIOPTERIS SP.) AND THEIR COMBINATION AGAINST PROPIONIBACTERIUM ACNES

2019 ◽  
pp. 11-13
Author(s):  
SUSI NOVARYATIIN
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
The Other ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Other Hand ◽  
Diffusion Technique ◽  
Inhibition Zones

Objective: The objective of this research was to investigate the preliminary phytochemical screening and antibacterial activity of Bawang Dayak(Eleutherine sp.) and Hati Tanah (Angiopteris sp.) and their combination against Propionibacterium acnes.Methods: The extracts were used for phytochemical screening. Antibacterial activity was performed using disc diffusion technique, with twovariations of the concentration of 5% and 10% for each extracts, and combination of both extracts with three combinations: (1) 5%: 5%, (2) 5%: 10%, and (3) 10%: 5%.Results: Both extracts contained tannins, saponins, and steroids. The antibacterial activity against P. acnes showed that the inhibition zones ofBawang Dayak ethanol extract were 6.1±1.5 mm (5%) and 8.7±1.3 mm (10%). On the other hand, the inhibition zones of Hati Tanah ethanol extract were 4.0±1.6 mm (5%) and 9.2±2.5 mm (10%). The inhibition zones produced in combinations 1, 2, and 3 were 5.8±0.3 mm, 10.8±2.0 mm, and 15.5±2.8 mm, respectively.Conclusion: In this study showed the presence of tannins, saponins, and steroids that might be responsible for antibacterial activity in both extract. The best antibacterial activity was produced by combining the two extracts (combination 3).

COMPARATIVE ASSESSMENT, PRELIMINARY SCREENING AND GC- FID PHYTOCHEMICAL ANALYSIS OF AQUEOUS AND PETROLEUM ETHER EXTRACTS OF PHYLLANTHUS AMARUS LEAVES.

2020 ◽  
Vol 45 (5) ◽  
Author(s):  
E.P Berezi ◽  
E. Mirinn ◽  
P.K. Berezi ◽  
A. E. Soroh
Keyword(s):  
Petroleum Ether ◽  
Total Concentration ◽  
Petroleum Ether Extract ◽  
Phytochemical Analysis ◽  
Phyllanthus Amarus ◽  
Ether Extract ◽  
Phytochemical Screening ◽  
Preliminary Screening ◽  
Traditional Remedies ◽  
Phytochemical Constituents

The Gas Chromatography– Flame lonization Detector (GC-FID) method was employed in this study to evaluate the phytochemical constituents of both the aqueous and petroleum ether extracts of phyllanthus amarus leaves. Preliminary phytochemical screening showed the presence of Alkaloids, Flavonoids Glycosides, Phenols, Saponins, and Tannins. Further analysis revealed a total concentration of Phenol as 82.95mg/100g (4.46%) in the aqueous extract, Tannins 722.77mg/100g(38.82%),Alkaloids was 466.82mg/100(25.07%) while in the petroleum ether extract, Flavonoids was 472.59mg/100g (29.50%), Saponins 12.60mg/100g (0.79%) and Glycosides 18.63mg/100g (1.16%). These results are suggestive that P.amarus is endowed with phytochemicals that contains therapeutic potencies which support its use as traditional remedies for the treatment of variety of ailments.

scholarly journals Phytochemical screening and hemolytique activity of some leaves extracts of Lantana camara L.

2021 ◽  
Vol 8 (3) ◽  
pp. 1-9
Author(s):  
Fahima Talhi ◽  
Noureddine Gherraf ◽  
Amar Zellagui ◽  
Awatif Boumaza ◽  
Amira Meghlaoui
Keyword(s):  
Phenolic Compounds ◽  
Hemolytic Activity ◽  
Methanolic Extract ◽  
Petroleum Ether Extract ◽  
Lantana Camara ◽  
Ether Extract ◽  
Phytochemical Screening ◽  
Long Time ◽  
Therapeutic Properties ◽  
Phytochemical Constituents

Abstract Medicinal plants have several therapeutic properties; they have been used for a long time to treat different diseases. Lantana camara L. has been widely used by man for healing these diseases. In this study, four leaves extracts of L. camara were subjected to preliminary phytochemical screening to determine the presence and/or the absence of phytochemical constituents; In addition, they were tested for hemolytic activity on human erythrocytes. This activity is performed using the UV-Vis spectrophotometer method at 520 nm and at five different concentrations (125 µg/ml, 250 µg/ml, 500 µg/ml, 750 µg/ml, and 1000 µg/ml). The phytochemical screening showed the presence of various phytochemical groups such as phenolic compounds, saponins, sterols, tannins, flavonoids, reducing compounds and the absence of alkaloids in the four extracts. These same extracts showed average hemolytic activity sequentially: chloroformic extract, petroleum ether extract, aqueous extract and then methanolic extract. This activity is dependent on the concentration of the extract.

scholarly journals COMPARATIVE STUDIES ON PHYTOCHEMICAL SCREENING OF FOUR DIFFERENT FLOWERS – COUROUPITA GUIANENSIS, BAUHINIA PURPUREA, STENOLOBIUM STANS, AND PLUMERIA RUBRA

2019 ◽  
pp. 119-121
Author(s):  
SAI NANDHINI R ◽  
NIRMALA NITHYA R
Keyword(s):  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Standard Procedure ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Flower Extract ◽  
Couroupita Guianensis ◽  
Bauhinia Purpurea ◽  
The Rich ◽  
Plumeria Rubra

Objective: The present study aims to investigate the phytochemical from the aqueous and methanol flower extracts of four significant medicinal plants, namely, Couroupita guianensis, Bauhinia purpurea, Stenolobium stans, and Plumeria rubra. Methods: The different flowers such as C. guianensis, B. purpurea, P. rubra, and S. stans were collected from Avadi and Koyambedu market, Chennai. Standard procedure was followed for the qualitative analysis of phytocompounds. Results: Preliminary phytochemical analysis resulted that the methanolic flower extract of four plants has rich bioactive compounds comparably to the aqueous flower extracts. Tannins, saponins, flavonoids, terpenoids, and steroids were highly present in B. purpurea compared the other three flowers. Only plant P. rubra contains the rich source of cardiac glycosides in methanolic flower extract. Conclusion: From the results of the current study, we conclude that the methanolic extract of B. purpurea flower has bulk phytocompounds and can be used as a traditional medicine to treat a variety of diseases.

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