scholarly journals Red dragon fruit (Hylocereus costaricensis Britt. Et R.) peel extract as a natural dye alternative in microscopic observation of plant tissues: The practical guide in senior high school

2017 ◽  
Vol 3 (3) ◽  
pp. 232
Author(s):  
Heni Wagiyanti ◽  
Rasuane Noor

Prepared slide of plant tissue needs to be staining to facilitate observations under microscope. Laboratorium activities in schools usually use synthetic dyes which expensive and can be damaged the student. Therefore the exploration of alternative dyes need to be established, such as utilizing of red dragon fruit (Hylocereus castaricensis Britt. Et R.). This study aims to (1) find out the best concentration of dragon fruit peel extract for staining plant tissue prepared slide and (2) to develop the practical guide related to plant tissue observation. The qualitative research used different concentration  of red dragon fruit peel extract, namely: 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100% with 3 repetitions. Data were obtained from observation photos of prepared slide. The result showed that the most contrast prepared slide was used red dragon fruit extract in 60% concentration. The result use to arrange practical guide in observation of plant tissues which is validated by material expert. The validation result showed “very good” criteria (86.01%).

2020 ◽  
Vol 6 (1) ◽  
pp. 22-25
Author(s):  
Mohamad Ikram ◽  
Nining Sugihartini ◽  
Any Guntarti

Red dragon fruit peel extract contains betalain which has antioxidant activity. In addition, this extract also contains dyes that can be used as coloring agents in cosmetic preparations. The purpose of this study was to study the activity of dragon fruit peel extract and red dragon fruit peel extract soap with various concentrations. Antioxidant activity is taken by the DPPH method. The concentration of red dragon fruit peel extract in soap is 0.3; 0.6; 0.9%. Test results show red dragon fruit extract soap has lower antioxidant activity than the extract. The increased concentration of the extract caused an increase in the antioxidant activity of the red dragon fruit peel extract soap.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Silaturahmi Silaturahmi ◽  
Zaidiyah Zaidiyah ◽  
Tengku Mia Rahmiati

The use of colorants in the dried noodle manufactures is an effort of product diversification. Besides of being used as a natural coloring agent, this peel extract is also used to improve nutritional value of the expected product. The purpose of this study was to determine the effect of red dragon fruit peel extract on the physical quality of dried noodle.  The study was conducted by using Completely Randomized Design (CRD) method with one factor, namely concentration of red dragon fruit peel extract (N1 = 10 ml, N2 = 15 ml, N3 = 20 ml, N4 = 25 ml, N5 = 30 ml).  The observations of its physical quality consisted of water absorption, solid loss during cooking, and organoleptic tests (aroma and color).  The best quality of dried noodle was obtained by using 30 ml red dragon fruit peel extract (N5) with physical properties, namely DSA levels of 351.92%, solid loss during cooking 4.78%, aroma 3.79 (like) and color 3.89 (like).


2019 ◽  
Vol 30 (4) ◽  
pp. 247
Author(s):  
Liana Liana ◽  
Rizal Rizal ◽  
Wahyu Widowati ◽  
Fioni Fioni ◽  
Khainir Akbar ◽  
...  

Aging is a natural process in human life and is triggered by the presence of free radicals (ROS). The use of antioxidants from natural ingredients is one of the breakthroughs to overcome aging and counteract the harmful effects caused by the free radicals. This study aimed to determine and compare the antioxidant activity of H2O2 scavenging and hyaluronidase inhibition of red dragon fruit peel extract (DFPE) and kaempferol-3-o-rutinoside (KOR) compounds. Dragon fruit peel extract (DFPE) is obtained through extraction by maceration method using 70% ethanol solvent. The design of this study included antioxidant and anti-aging activity assay of EKBN and KOR at the series concentration of 15.63; 31.25; 62.50; 125; 250; 500 µg/mL through H2O2 scavenging, as well as the DFPE and KOR hyaluronidase inhibition assay at the series concentration of 5.21; 10.42; 20.83; 41.7; 83.33; 166.67 µg/mL. EKBN shows that the average activity of H2O2 scavenging is lower than KOR. In addition, the IC50 values of KOR for H2O2 scavenging is lower (351.46±2.30ug/mL) than DFPE (409.64±23.17ug/mL). While, KOR also has higher values of inhibitory activity than of the DFPE. However, the IC50 value of KOR for hyaluronidase inhibition activity was 84.07±10.46µg/mL, equivalent to the IC50 value of DFPE (85.32±10.24µg/mL). The presence of antioxidant and anti-aging activity in the EKBN is probably caused by betalain and the KOR compound itself contained in red dragon fruit. The results of the paired-samples T-test on antioxidant activity and anti-aging of DFPE and KOR showed non-significant difference. Thus, DFPE has an equivalent antioxidant and anti-aging through H2O2 scavenging and hyaluronidase activity as possessed by the KOR compound.


2018 ◽  
Vol 12 (3) ◽  
pp. 337
Author(s):  
Hadi Barru Hakam Fajar Siddiq ◽  
Agnis Pondinekaria Aditama

Community service activities undertaken by lecturers from Pharmacy Diploma III Program of Pharmacy Academy of Jember held on March, 2018, held at MA. Habiburrahman Jatian, Pakusari Jember provides benefits for students in improving students skill through soap red dragon fruit extract. MA. Habiburrahman was senior high school under the Ministry of Religious Affairs located in Jatian-Pakusari-Jember. MA. Habibirrahman also has a religious education institution that is Pondok Pesantren Habibirrahman. Nearly 70% of students of MA. Habiburrahman were santri. The results show that MA Habiburrahaman does not have a curculum that focuses on the attachment of students. Therefore, this program provides many benefits to students to add soft skills in the field of technology. In addition, this program is in great demand by all students. Keywoard: Community Servive, Soft Skill, Soap, Red Dragon Fruit


KOVALEN ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 280-289
Author(s):  
Amalia Noviyanty ◽  
Chitra Anggriani Salingkat ◽  
Syamsiar Syamsiar

This study aims to determine the effect of the ratio of solvents to extract yield, phenolics total and IC50 values ​​of red dragon fruit peel and also get the optimal solvent ratio to obtain extract yield, phenolics total and the highest or best IC50 values ​​of red dragon fruit peel extracts. The solvent ratio used there are 6 levels of the ratio: 2:1; 3:1; 4:1; 5:1; 6:1 and 7:1 (v/w). The data obtained were analyzed using a Completely Randomized Design that was applied to observations of extract yield, phenolics total and IC50 values, if the treatment had a very significant or significant effect followed by continued Tukey HSD test at 1% or 5% level. The results showed that the solvent ratio very significantly affected the extract yield, phenolics total and IC50 value of red dragon fruit peel extract.  The solvent ratio of 4:1 (v/w) produced extract yield, phenolics total and IC50 values the highest or best i.e  26.22%, 71.56 ppm, and 124.62 ppm. Keywords : IC50, the red dragon fruit skin, solvent ratio, phenolics total


2021 ◽  
Vol 6 (2) ◽  
pp. 154
Author(s):  
Maydina Izzatul Yazidah ◽  
Didit Aspriyanto ◽  
Renie Kumala Dewi

Background: Radiation exposure from periapical imaging can cause cell damage in various tissues due to the formation of free radicals. Cell damage in the tissue can be observed from the number of neutrophils which are immune cells. Free radicals can be suppressed by administering exogenous antioxidants. One source of natural antioxidants is red dragon fruit, especially on its peel. Objective: To analyze the effect of periapical imaging x-ray radiation exposure after the administration of red dragon fruit peel extract on absolute neutrophil counts in mice. Method: This research was a true experimental study with a post-test only and control group design using 24 mice that were divided into 6 groups. The control group was not given any treatment while P1 to P5 group were given red dragon fruit peel extract at a dose of 100 mg/kg BW and exposed to radiation with different exposures. P1 group was exposed 1 time (0.63 mGy), P2 group was exposed 4 times (1.66 mGy), P3 group was exposed 8 times (4.37 mGy), P4 group was exposed 12 times (8.19 mGy), and group P5 was given no exposure. Results: There were changes in the number of neutrophils in all groups. The LSD Posthoc test results showed a significant difference between the control group (12.470 / mm3) and the P5 group (12.470 / mm3) compared to P3 group with 8 times exposure (40.348 / mm3). Conclusion: There is an effect of periapical radiographic x-ray radiation exposure by red dragon fruit peel extract on the absolute neutrophil count in mice. Keywords: Antioxidants, Neutrophils, Periapical Radiography, Radiation, Red Dragon Fruit Peel.


Author(s):  
Adilla Nur Azizah ◽  
Putri Agustina ◽  
Suparti Suparti ◽  
Alanindra Saputra ◽  
Yasir Sidiq

One of the Basic Competencies (KD) in Biology for Indonesian Senior High School is KD 4.12 which reads "Presents observations of the process of mitosis in the onion root and determines the phases it finds". Based on the basic competency test, students are required to have skills in making and observing mitotic preparations of plant tissue with at least using onion. Observation of mitotic division in plant tissues requires skill in making preparations. Making preparations in observing cells and tissues of plants or animals needs staining. Dyes often used in lab work are synthetic dyes. One of the synthetic dyes commonly used is safranin. However, safranin is quite expensive. This limitation requires teachers to look for alternatives to synthetic dyes that are easily found at a more affordable price, namely with natural dyes. One of the plants that has the potential to overcome this issue is beetroot (Beta vulgaris). The purpose of this study was to determine the quality of plant tissue preparations (Allium cepa) stained with beetroot skin (Beta vulgaris) as a natural coloring agent. The research method used was an experimental method with a Completely Randomized Design consisting of two factors, namely the staining time (1 hour, 2 hours, and 3 hours) and the type of solvent (aquades and citric acid 10%). The results of the study were analyzed with qualitative descriptive methods which included contrast and clarity of the preparations. Based on the results of the study, the quality of Allium cepa root preparations using beetroot (Beta vulgaris) skin extract with variations in the type of solvent and duration of staining showed good results on the staining time of 1 hour and 2 hours. The staining time of the preparations can affect the contrast and clarity of the preparations.


KOVALEN ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 191-197
Author(s):  
Sucianti ◽  
Nurhaeni ◽  
Jaya Hardi

Red super dragon fruit peel (Hylocereus costaricensis) extract has capability to scavenge DPPH and the activity could be maintained by using maltodextrin as coating. The aims of the research are to determine ratio of red super dragon fruit peel ethanol extract toward maltodextrin producing microcapsule with the highest coating efficiency and DPPH scavenging content by microcapsule of red super dragon fruit peel extract. Microencapsulation was carried out using freeze drying technique. Comparison of red super dragon fruit peel extract and maltodextrin were 1: 8, 1: 10, 1: 12, and 1:14 (w / w). The results show that the highest efficiency ratio of coating extract of red super dragon fruit peel with maltodextrin was 1: 8 with percentage of 90,77%. Level of DPPH scavenging content of red super dragon fruit peel extract microcapsule with ratio of 1:8 on 5 minutes and 60 minutes each is 27.36% and 46.62%. Keywords: Hylocereus costaricensis, freeze drying, maltodextrin, microencapsulation


Author(s):  
Norasiha Hamid ◽  
Mimi Sakinah Abdul Munaim ◽  
Mazrul Nizam Abu Seman

The betacyanin pigment extracted from the dragon fruit peel has a potential to be a natural dye as an alternative to replace the synthetic dyes. To investigate the dyeability of spun silk with betacyanin pigment, the adsorption isotherm models were performed. The equilibrium adsorption data were analyzed using the Langmuir, Freundlich, and Temkin isotherm models. In order to determine the best-fit isotherm for each system, three error analysis methods were used to evaluate the data, namely the sum of the squares of the errors, residual root mean square error and chi-square test. On the basis of low three error analysis and high correlation of determination, it was found that the Langmuir isotherm model fitted well with the experimental data. Therefore, it can be concluded that the adsorption process of betacyanin pigment onto the spun silk followed the Langmuir isotherm model. Moreover, the adsorption features of the experimental system might be caused by the monolayer adsorption.


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