scholarly journals Toxicological Impact of Leachates from Lemna Refuse Dump Site on African Catfish (Clarias gariepinus, Burchell 1882) Fingerlings Under Laboratory Condition

2021 ◽  
pp. 23-37
Author(s):  
Udeme Uyom Udofia ◽  
Finian Tobias Okoro ◽  
Akaninyene Paul Joseph
Keyword(s):  
Block Design ◽  
Clarias Gariepinus ◽  
Aquatic Systems ◽  
Control Group ◽  
African Catfish ◽  
Dump Site ◽  
Toxicological Effects ◽  
Lc50 Value ◽  
Randomized Block Design ◽  
Set Up

Aim: The study investigated the toxicological effects of Calabar municipal dumpsite leachate on the fingerlings of Clarias gariepinus. Experimental Design: The study was set-up in a 6 × 2 Complete Randomized Block Design. Methodology: During the studt 120 C. gariepinus fingerlings were used. Each group was made up of 10 fingerlings. The fingerlings were exposed to 0% (control group), 15%, 20%, 25%, 30% and 35% of leachate in duplicate. Histopathology was also carried-out on the gills and liver of the fingerlings of each exposure group. Results: The temperature, pH, conductivity, and BOD increased with increasing leachate concentration, while the DO decreased with concentration. 5.17 ± 0.66 cm and 1.09 ± 0.40 g mean length and weight respectively of fingerlings were determined. Mortality depended on concentration. The 96 hours LC50 value with 95% confidence limit of C. gariepinus fingerlings exposed to leachate was 22.5% ± 0.89, and was significant with a determination coefficient (r2) of 0.93 at P<0.05. Leachates altered the orientation of gills and liver of the fingerlings. Conclusion and Recommendations: The low LC50 value of fingerlings exposed to different concentrations of leachate indicates a high toxicity of the leachate and altered the gills and liver of fingerlings. To this end, we recommended dumpsites are sited at a distance far from water bodies and areas inhabited by human, to mitigate the leeching of leachate into nearby aquatic systems. Law against indiscriminate discharge of waste into drainage channels and any aquatic system should be enforced by Government, to prevent mortality of biological organisms, extinction of species, contamination of organisms in nearby aquatic systems, changes in the physical properties, and health risk to humans that depends on resources from these aquatic systems for food.

scholarly journals Efficacy of Aqueous Crude Fruit Extract of Desert Date (Balanites aegyptiaca) in Anaesthetization of African Catfish (Clarias gariepinus) Fingerlings

2020 ◽  
Vol 10 (2) ◽  
pp. 45-52
Author(s):  
Idi Audu Wakawa ◽  
Abubakar Chiroma
Keyword(s):  
Tap Water ◽  
Block Design ◽  
Clarias Gariepinus ◽  
Total Alkalinity ◽  
African Catfish ◽  
Fruit Extract ◽  
Balanites Aegyptiaca ◽  
Randomized Block Design ◽  
Free Carbon Dioxide ◽  
Anaesthetic Effect

Chemicals have been used to anaesthetize fish but due to their hazardous effects on the environment, fish and humans environmentally friendly plant anaesthetics are being sought. Fruit of Desert date (Balanites aegyptiaca) is nontoxic to humans but has been reported to have anaesthetic potentials on fish. This study investigates anaesthetic effect of aqueous crude fruit extract of B. aegyptiaca on African catfish (Clarias gariepinus) fingerlings. Phytochemical and proximate compositions of the fruit were screened. Exactly 120 C. gariepinus fingerlings (mean weight 32.13±2.43g and mean total length 23.88±2.11cm) were used for the experiment. A total of 10 fingerlings were exposed to each of 2.00, 2.50, 3.00, 3.50 and 4.00g/L concentrations of aqueous crude fruit extract of B. aegyptiaca and a control in 6 plastic tanks (45x28x25cm) filled with 10L of tap water. Setup was arranged in randomized block design and replicated. Temperature, dissolved oxygen, pH, free carbon dioxide and total alkalinity were monitored. Results revealed long mean induction (25.05±3.35 min) and recovery (108.35±2.45 min) times with resultant mortalities (40%) of C. gariepinus fingerlings. significant correlation (P<0.01) exists between concentration of the fruit extract and induction time as well as between fruit extract concentrations and survival of fingerlings. Aqueous crude fruit extract of B. aegyptiaca is, therefore, not effective for anaesthetization of C. gariepinus fingerlings hence should be avoided. Keywords: Anaesthetic, Balanites aegyptiaca, Clarias gariepinus

scholarly journals Agronomic performances of Pak Choi grown with different soil cover

2015 ◽  
Vol 33 (2) ◽  
pp. 261-266 ◽  
Author(s):  
Márcia M Echer ◽  
Graciela M Dalastra ◽  
Tiago L Hachmann ◽  
Elcio S Klosowski ◽  
Vandeir F Guimarães
Keyword(s):  
Soil Cover ◽  
Block Design ◽  
Dry Weight ◽  
Bare Soil ◽  
Production Traits ◽  
Pak Choi ◽  
Randomized Block Design ◽  
Black Plastic ◽  
Growing Conditions ◽  
Set Up

An important aspect in the cultivation of vegetables is the quality of the product to be marketed, free from dirt and damage and the practice of mulching could be an option, but there is scarse information. The aim of this study was to evaluate the influence of mulching on the production traits of three cultivars of Pak Choi. The experiment was set up during March to May 2013 using a split plot randomized block design, with four replications. Soil cover treatments (white agrotextile, black agrotextile, black plastic, silver plastic, tifton straw and bare soil) were arranged in the plots, and three cultivars of Pak Choi (Green Pak Choi, White Pak Choi and Chingensai Natsu Shomi) in subplots. Height and diameter of shoots, number of leaves, fresh weight of the head and petiole, base diameter, dry weight of stem, petiole, and leaf were evaluated thirty-five days after transplantation. The total dry weight and leaf area were measured, and then we estimated the yield. There was a significant effect of soil cover and cultivar. In general, the cover with synthetic materials showed higher values on production of Pak Choi. The cultivar White Pak Choi was better adapted to the growing conditions, with an average yield of 57.78 t/ha.

scholarly journals Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra
Keyword(s):  
Carica Papaya ◽  
Clonal Propagation ◽  
Block Design ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Randomized Block Design ◽  
Lateral Shoots ◽  
Shoot Production ◽  
Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

scholarly journals Interference of Urochloa plantaginea on Morphophysiology and Yield Components of Black Beans

2019 ◽  
Vol 11 (9) ◽  
pp. 272
Author(s):  
Milena Barretta Franceschetti ◽  
Leandro Galon ◽  
Maico André Michelon Bagnara ◽  
Renan Pawelkiewicz ◽  
Leonardo Brunetto ◽  
...  
Keyword(s):  
Block Design ◽  
Plant Morphology ◽  
Co2 Concentration ◽  
Crop Productivity ◽  
Dry Mass ◽  
Control Group ◽  
Total Period ◽  
Black Beans ◽  
Physiological Variables ◽  
Randomized Block Design

Among the factors which affect crop productivity, there are weeds which compete for environment resources. The objective of this work was to determine the periods of interference of alexandergrass on the morphophysiology and productivity components of black beans. The experiment was conducted in a complete randomized block design with four replications. The bean cultivar of the black type used was IPR Uirapuru, being the treatments separated in two models of interference: in the coexistence group, the crop of the bean lived with the alexandergrass by growing periods of 0, 7, 14, 21, 28, 35, 42 and throughout the cycledays after emergence and throughout the cycle; in the control group the crop was kept free of the infestation for the same periods described previously. At 42 DAE were evaluated the variables related to plant morphology and physiology (height of bean plants, number of trifoliate leaves, dry mass plant, number of grains pods, number od pods plants, stomatal conductance, internal CO2 concentration, photosynthetic activity, efficiency of carboxylation, efficient use of water, transpiration rate). Considering the results, it is possible to conclude that the critical period of interference prevention (CPIP) comprises 24 to 50 DAE, the period before the interference (PBI) was 24 DAE and the total period of interference prevention (TPIP) was 50 days and that the morphological and physiological variables of the plants were negatively affected in the coexistence with the alexandergrass when compared to the treatments kept free of weeds throughout the crop cycle.

scholarly journals PERBEDAAN PENURUNAN TEKANAN DARAH PRA LANSIA PRE HIPERTENSI YANG DIBERI JUS TOMAT (Lycopersicum grandifilum)

2018 ◽  
Vol 10 (2) ◽  
pp. 152-158
Author(s):  
Hari Yani Fadillah
Keyword(s):  
Blood Pressure ◽  
Treatment Group ◽  
Block Design ◽  
Pressure Control ◽  
Tomato Juice ◽  
Control Group ◽  
Randomized Block Design ◽  
Before And After ◽  
Blood Pressure Treatment ◽  
And Control

The research aims to know the effect of tomato juice on blood pressure in pre-elderly pre hypertension at Posbindu Puskesmas Nusa Indah Bengkulu City.The design of research was a quasi experiment include pre test post test. This population was all patients posbindu aged 45-59 years. Sampling of this research randomized block design of one factorial were 6 samples divided into treatment and control group. The data which have been analyzed using univariate and bivariate. From this research were obtained the results such as the mean of sistole blood pressure before and after intervention in treatment group p = 0,273 and diastole p = 0,27. Mean systole blood pressure control group p = 0.060 and diastole p = 2.14. Distribution of tomato juice (Lycopersicum grandifilum) did not given affect the decrease in blood pressure of systole diastole before and after intervention in pre-elderly pre hypertension. Whereas the difference in mean systole of blood pressure treatment and control group p = 0.935 and diastolic of blood pressure p = 0.678. There ware not difference in mean of blood pressure in the tomato juice treatment group and control of Vitamin C. The result concluded there were not effect of tomato juice (Lycopersicum grandifilum) on systolic of blood pressure in pre-elderly pre hypertension at Posbindu Puskesmas Nusa Indah Bengkulu City.

scholarly journals BLOOD INDICES OF AFRICAN CATFISH (CLARIAS GARIEPINUS) FOLLOWING DIETARY ADMINISTRATION OF TALINUM TRIANGULARE

2019 ◽  
Vol 7 (4) ◽  
pp. 185-198 ◽  
Author(s):  
Tina Porwal
Keyword(s):  
Blood Cell ◽  
Clarias Gariepinus ◽  
Control Group ◽  
African Catfish ◽  
Animal Blood ◽  
Blood Indices ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Leaf Powder ◽  
Talinum Triangulare

The effects of Talinum triangulare on the haematological indices of Clarias gariepinus was investigated. T. triangulare leaf powder at 0, 0.5, 1.0, and 3.0% concentrations were fed to Clarias gariepinus juveniles (n=84; 117.3 ± 1.57 g; 26.70 ± 0.26 cm) as feed additive for a period of six (6) weeks.  The packed cell volume (PCV) and Haemoglobin (Hb) concentration values, 34.67 ± 0.67%, 12.03 ± 0.26g/dl, respectively observed in the group fed with 1.0% were significantly higher (P<0.05) compare with the control group. Red blood cell counts (RBCs), white blood cell counts (WBCs), lymphocytes, MCV, MCH and MCHC recorded were marginally different (P>0.05) among the groups, were within standard for Clarias gariepinus, therefore adjudged not to indicate a negative physiological effect on the experimental fish. Blood indices values observed revealed that the varying additive levels of T. triangulare used had no negative physiological stress on the health status of the fish studied and haematological values observed were seen to be best in the group fed 1.0% T. triangulare. It could be recommended that 10g/kg of Talinum triangulare leaf powder be included in the diet of Clarias gariepinus for boosting of the animal blood and treatment of disease conditions such as anaemia.

scholarly journals The Effect of Writing Therapy on Anxiety in Pregnant Women: A Randomized Controlled Trial

2020 ◽  
Vol 14 (2) ◽  
Author(s):  
Maryam Montazeri ◽  
Khalil Esmaeilpour ◽  
Sakineh Mohammad-Alizadeh-Charandabi ◽  
Sevda Golizadeh ◽  
Mojgan Mirghafourvand
Keyword(s):  
Randomized Controlled Trial ◽  
Pregnant Women ◽  
Controlled Trial ◽  
Block Design ◽  
Intervention Group ◽  
Control Group ◽  
Randomized Controlled ◽  
Writing Therapy ◽  
Randomized Block Design ◽  
Significant Difference

Background: As one of the most enjoyable life experiences, pregnancy may be accompanied by many physiological and psychological changes that make women susceptible to developing mental disorders such as anxiety. Non-pharmacological methods such as writing therapy are among the ways to deal with anxiety. Objectives: The present study aimed to determine the effect of writing therapy on anxiety in women during pregnancy. Methods: This randomized controlled trial was conducted on 70 pregnant women with a gestational age between 28 - 31 weeks. The participants were assigned to an intervention group and a control group using a randomized block design. The intervention group received three in-person writing therapy-based counseling sessions and two telephone calls between the sessions, while the control group received routine pregnancy care. The Beck anxiety inventory was completed before and six weeks after the intervention. Independent t-test and ANCOVA were used for data analysis. Results: There was no significant difference between the two groups in terms of socio-demographic variables and baseline anxiety scores. Based on ANCOVA with baseline score adjustment, the mean score of anxiety was significantly lower in the intervention group than in the control group after the intervention (adjusted mean difference = -6.8; 95%CI = -4.5 to -9.1; P < 0.001). Conclusions: According to the results, writing therapy can reduce anxiety in pregnant women; however, further studies are needed before a definitive conclusion.

scholarly journals Organic fertilization to attenuate water salinity effect on papaya growth

2019 ◽  
Vol 23 (2) ◽  
pp. 79-83
Author(s):  
Mônica S. da S. Sousa ◽  
Vera L. A. de Lima ◽  
Marcos E. B. Brito ◽  
Luderlândio de A. Silva ◽  
Rômulo C. L. Moreira ◽  
...  
Keyword(s):  
Irrigation Water ◽  
Saline Water ◽  
Block Design ◽  
Fruit Trees ◽  
Water Salinity ◽  
Organic Fertilization ◽  
Randomized Block Design ◽  
Irrigation Water Salinity ◽  
Set Up ◽  
Management Alternatives

ABSTRACT The salinity of water and soil reduces the growth and production of crops, especially the fruit trees, such as papaya. Thus, it is necessary to obtain management alternatives for cultivation under these conditions. Therefore, the objective of this study was to evaluate the growth and phytomass of papaya cultivated under irrigation with saline water and organic fertilization. An experiment was set up using a randomized block design, with the treatments distributed in a 5 x 2 factorial scheme, consisting of five levels of salinity of irrigation water (0.6, 1.2, 1.8, 2.4 and 3.0 dS m-1) and two levels of organic fertilization (10 and 20 L of bovine manure per plant), with three replications, totaling thirty experimental plots. Growth variables of papaya were evaluated. Papaya plants were negatively affected by irrigation water salinity, with a greater effect on the number of leaves and on dry phytomass of leaves, with no effect of bovine manure levels.

OCDs in Randomized Block Design Set-Up

2015 ◽  
pp. 27-39
Author(s):  
Premadhis Das ◽  
Ganesh Dutta ◽  
Nripes Kumar Mandal ◽  
Bikas Kumar Sinha
Keyword(s):  
Block Design ◽  
Randomized Block Design ◽  
Set Up

scholarly journals Potential use of ash from sugarcane bagasse to produce passion fruit seedlings

2020 ◽  
pp. 408-414
Author(s):  
Henrique Guimarães de Favare ◽  
Sandra da Costa Preisigke ◽  
Leonarda Grillo Neves ◽  
Kelly Lana Araújo ◽  
Milson Evaldo Serafim ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Soil Structure ◽  
Block Design ◽  
Dry Mass ◽  
Passion Fruit ◽  
Randomized Block Design ◽  
The Family ◽  
Set Up ◽  
Physical And Chemical ◽  
Potential Use

The production of good quality passion fruit seedlings depends on substrates with adequate physical and chemical characteristics. Therefore, this experiment was conducted to evaluate the effect of doses of ash from burning of sugarcane bagasse on improvement of traits of the substrate "gully soil". The experiment was set up in a greenhouse and arranged in randomized block design, in a 2x7x2 factorial scheme. The following factors and their respective levels were studied: (i) soil structure,- aggregating between 4 and 10 mm and <2 mm; (ii) ash doses - 0; 1.5; 3; 6; 12; 24; and 48 t ha-1; (iii) families of passion fruit - F29 and F48. The use of ash combined to the soil with structure <2mm significantly increased the production of shoot fresh mass and shoot dry mass for both families studied. The density of the substrate obtained by the mixture of ash and gully soil (soil) decreased as ash doses increased, regardless of the size of the aggregates and the family studied.

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