scholarly journals Immunostimulant Effect of Seaweeds in Channa punctatus Challenged by Aeromonas hydrophila

2021 ◽  
Vol 11 (4) ◽  
pp. 20-23
Author(s):  
S Muthu Krishnan ◽  
P Raja
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Channa Punctatus ◽  
Aqueous Extracts ◽  
Fish Pathogen ◽  
Inhibitory Zone ◽  
Active Metabolites ◽  
Seaweed Extracts ◽  
Challenge Study ◽  
Bioactive Potential

The current study focused on to evaluate the bioactive potential and immunostimulant effect of seaweeds in striped murrel Channa punctatus against the fish pathogen Aeromonas hydrophila. The aqueous extracts of seaweeds, Glacilaria foliifera, Acanthophora spicifera and Ulva lactuca were obtained in four different concentrations (25 µL, 50 µL, 75 µL and 100 µL) and evaluated for their immunostimulant potential.  Aqueous extract of G. foliifera and A. spicifera has shown promising activity against fish pathogen. G. foliifera exhibited higher bacterial inhibitory zone of 8mm at 100 µL concentration followed by A. spicifera with 6mm at same concentration. The disease challenge study was used to ascertain the immunostimulant efficacy of active seaweed extracts. C. punctatus was administrated with the A. hydrophila and then fed with artificial feed incorporated with seaweeds extract.  The survival rate seems to be higher in G. foliifera (80%) when compared with A. spicifera (60%). Observed result shows that both seaweeds possess active metabolites which may pave the way for the discovery of novel compounds with immunostimulant potential, which can be used in the field of aqua industry to augment disease management. Keywords: Seaweed, Bioactive compounds, Aeromonas hydrophila, Gracilaria polifera

The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

2014 ◽  
pp. 27
Author(s):  
Arne A Ratulangi ◽  
Reiny Tumbol ◽  
Hengky Manoppo ◽  
Henneke Pangkey
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Bacterial Disease ◽  
Immersion Method ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Different Ages ◽  
Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

scholarly journals Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Olivia Nathalia ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Quorum Quenching ◽  
Indicator Bacteria ◽  
Antibiofilm Activity ◽  
Fish Pathogen ◽  
Phyllosphere Bacteria

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

Disruption of the quorum sensing regulated pathogenic traits of the biofilm-forming fish pathogen Aeromonas hydrophila by tannic acid, a potent quorum quencher

Biofouling ◽  
2017 ◽  
Vol 33 (7) ◽  
pp. 580-590 ◽  
Author(s):  
Bhakti Patel ◽  
Supriya Kumari ◽  
Rajanya Banerjee ◽  
Mrinal Samanta ◽  
Surajit Das
Keyword(s):  
Quorum Sensing ◽  
Tannic Acid ◽  
Aeromonas Hydrophila ◽  
Fish Pathogen

scholarly journals Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus)

F1000Research ◽  
2019 ◽  
Vol 7 ◽  
pp. 1847 ◽  
Author(s):  
Esti Handayani Hardi ◽  
Rudy Agung Nugroho ◽  
Irawan Wijaya Kusuma ◽  
Wiwin Suwinarti ◽  
Agung Sudaryono ◽  
...  
Keyword(s):  
Survival Rate ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Prevention Trial ◽  
Phagocytic Index ◽  
Immunological Parameters

Background: The combination of some plant extracts to prevent and treat bacterial infections is gaining momentum, because of effectiveness against certain bacteria. This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zingiber Zerumbet (ZZ) plant extracts to treat and prevent Aeromonas hydrophila and Pseudomonas fluorescens infection on Tilapia (Oreochromis niloticus). Methods: Tilapia (initial weight 15±2 g) were injected intramuscularly (0.1 ml/fish) with a combination of A. hydrophila and P. fluorescens at a density of 1×10 5 CFU ml -1 of each bacteria. Treatment trials were performed at day 7 post-injection with each combined extract, while the prevention trial was performed by including the combined extract into the commercial diet for six and seven days prior to injection. Various extract combinations were 60 mg SF extract/kg feed with 40 mg ZZ/kg feed (SF60/ZZ40), SF50/ZZ50, BP90/SF10, and BP50/SF50. Haemato-immunological parameters were performed for four weeks. Results: In prevention trials, tilapia fed SF50/ZZ50 showed a significant increase of white and red blood cells. Similarly, significantly increased haematocrit was found in tilapia fed SF50/ZZ50 in the treatment trial but not in the prevention trial. In both trials, haemoglobin of tilapia was not affected by any combined extracts but decreased the number of bacteria. Phagocytic index, respiratory burst, lysozyme activity and survival rate of fish fed combined extracts were found significantly higher than controls. The amount of pathogenic bacteria in fish fed combined extracts was lower than the control at week 4 (P<0.05). In both trials The percentage of survival rate and relative percent survival of tilapia fed SF 50/ZZ 50, showed the optimum results compared to the other combinations. Conclusions: The combined extract in feed, especially SF50/ZZ50 has a positive effect on the tilapia's innate immune system of tilapia to treat and prevent bacterial infections.

scholarly journals The influence of the mangrove leaves infusion Excoecaria agallocha against Aeromonas hydrophila bacteria infection in tilapia

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Dian Puspitasari
Keyword(s):  
Weight Gain ◽  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Control Group ◽  
Fish Disease ◽  
Phytochemical Screening ◽  
Control Group Design ◽  
Group Design ◽  
Excoecaria Agallocha ◽  
New Antibiotics

Tilapia is fish consumption with some excess, that is has a high protein, non-cholesterol, the taste is delicious, affordable and easily cultivated. One of the problems in cultivating them is a disease because it can cause the death of the fish. Disease-causing factors can be derived from the bacterium Aeromonas hydrophila. The bacteria A. hydrophila can cause the death of fish until 80%-100% within 1-2 week and even many that are already resistant to several types of commercial antibiotics, so that the need to look for a new antibiotics source. The source of these antibiotics can be derived from nature because it is cheaper, safer and environmentally friendly. Mangrove Excoecaria agallocha has been known to have potential as an antibacterial, larvacide, and antifungal. This can be used as a source of antibiotics. The purpose of this research is to analyze the potential of the mangrove leaves infusion E. agallocha in preventing the spread of disease in tilapia infected the bacteria and identify the compounds of secondary metabolite. This research was carried out from March-August 2018 and use approach posttest-only control group design (by comparing the treatment with the control group). The amount of treatment is five with three replicates. The dose used, namely 0%, 2.5%, 5%, 7.5% and 10%. The results showed that there was no difference in the influence of the mangrove leaves infusion E. agallocha against weight gain and survival rate of tilapia juveniles. The result of phytochemical screening indicates that E. agallocha leaves infusion contains tannins and flavonoids.

Sign in / Sign up

Export Citation Format

Share Document