scholarly journals The Effect of Adding Bacillus NP5 to Feed on Growth, Survival Rate, and Protection Against Aeromonas hydrophila of Catfish (Clarias sp.)

2021 ◽  
Vol 715 (1) ◽  
pp. 012058
Author(s):  
Mustahal ◽  
Sevia ◽  
M Herjayanto ◽  
M B Syamsunarno ◽  
A N Putra
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila

The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

2014 ◽  
pp. 27
Author(s):  
Arne A Ratulangi ◽  
Reiny Tumbol ◽  
Hengky Manoppo ◽  
Henneke Pangkey
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Bacterial Disease ◽  
Immersion Method ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Different Ages ◽  
Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

scholarly journals Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus)

F1000Research ◽  
2019 ◽  
Vol 7 ◽  
pp. 1847 ◽  
Author(s):  
Esti Handayani Hardi ◽  
Rudy Agung Nugroho ◽  
Irawan Wijaya Kusuma ◽  
Wiwin Suwinarti ◽  
Agung Sudaryono ◽  
...  
Keyword(s):  
Survival Rate ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Prevention Trial ◽  
Phagocytic Index ◽  
Immunological Parameters

Background: The combination of some plant extracts to prevent and treat bacterial infections is gaining momentum, because of effectiveness against certain bacteria. This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zingiber Zerumbet (ZZ) plant extracts to treat and prevent Aeromonas hydrophila and Pseudomonas fluorescens infection on Tilapia (Oreochromis niloticus). Methods: Tilapia (initial weight 15±2 g) were injected intramuscularly (0.1 ml/fish) with a combination of A. hydrophila and P. fluorescens at a density of 1×10 5 CFU ml -1 of each bacteria. Treatment trials were performed at day 7 post-injection with each combined extract, while the prevention trial was performed by including the combined extract into the commercial diet for six and seven days prior to injection. Various extract combinations were 60 mg SF extract/kg feed with 40 mg ZZ/kg feed (SF60/ZZ40), SF50/ZZ50, BP90/SF10, and BP50/SF50. Haemato-immunological parameters were performed for four weeks. Results: In prevention trials, tilapia fed SF50/ZZ50 showed a significant increase of white and red blood cells. Similarly, significantly increased haematocrit was found in tilapia fed SF50/ZZ50 in the treatment trial but not in the prevention trial. In both trials, haemoglobin of tilapia was not affected by any combined extracts but decreased the number of bacteria. Phagocytic index, respiratory burst, lysozyme activity and survival rate of fish fed combined extracts were found significantly higher than controls. The amount of pathogenic bacteria in fish fed combined extracts was lower than the control at week 4 (P<0.05). In both trials The percentage of survival rate and relative percent survival of tilapia fed SF 50/ZZ 50, showed the optimum results compared to the other combinations. Conclusions: The combined extract in feed, especially SF50/ZZ50 has a positive effect on the tilapia's innate immune system of tilapia to treat and prevent bacterial infections.

scholarly journals The influence of the mangrove leaves infusion Excoecaria agallocha against Aeromonas hydrophila bacteria infection in tilapia

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Dian Puspitasari
Keyword(s):  
Weight Gain ◽  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Control Group ◽  
Fish Disease ◽  
Phytochemical Screening ◽  
Control Group Design ◽  
Group Design ◽  
Excoecaria Agallocha ◽  
New Antibiotics

Tilapia is fish consumption with some excess, that is has a high protein, non-cholesterol, the taste is delicious, affordable and easily cultivated. One of the problems in cultivating them is a disease because it can cause the death of the fish. Disease-causing factors can be derived from the bacterium Aeromonas hydrophila. The bacteria A. hydrophila can cause the death of fish until 80%-100% within 1-2 week and even many that are already resistant to several types of commercial antibiotics, so that the need to look for a new antibiotics source. The source of these antibiotics can be derived from nature because it is cheaper, safer and environmentally friendly. Mangrove Excoecaria agallocha has been known to have potential as an antibacterial, larvacide, and antifungal. This can be used as a source of antibiotics. The purpose of this research is to analyze the potential of the mangrove leaves infusion E. agallocha in preventing the spread of disease in tilapia infected the bacteria and identify the compounds of secondary metabolite. This research was carried out from March-August 2018 and use approach posttest-only control group design (by comparing the treatment with the control group). The amount of treatment is five with three replicates. The dose used, namely 0%, 2.5%, 5%, 7.5% and 10%. The results showed that there was no difference in the influence of the mangrove leaves infusion E. agallocha against weight gain and survival rate of tilapia juveniles. The result of phytochemical screening indicates that E. agallocha leaves infusion contains tannins and flavonoids.

scholarly journals The Influence of Binahong (Anredera cordifolia) Leaf Powder Addition to Feed on the Prevalence and Survival Rate of Red Tilapia (Oreochromis niloticus) seeds Infected by Aeromonas hydrophilla Bacteria

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (3) ◽  
pp. 144
Author(s):  
Juliana Juliana ◽  
Yuniarti Koniyo ◽  
Citra Panigoro
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Total Body Weight ◽  
Fish Feed ◽  
Red Tilapia ◽  
Leaf Powder ◽  
Powder Addition ◽  
Total Body ◽  
Main Factor

Feed is one main factor in aquaculture activities which influences the quantity and quality of aquaculture commodities. Feed can also increase the cultivated fish survivability to prevent from fish diseases. Binahong leaf powder is one material added in the fish feed making which contains active compounds functioning as antibacterial agent. This study did aim at examining the influence of Binahong leaf powder addition to feed on the prevalence and survival rate of Red Tilapia seeds infected by Aeromonas hydrophila bacteria. This research employed an experimental method using a randomized complete design with four treatments. Each treatment consisted of different Binahong leaf powder dosage used as the material in feed making with treatment A (5%), treatment B (7%), treatment C (9%), and treatment D (control). The test animals were ± 5 cm red tilapia seeds infected by Aeromonas hydrophila bacteria. Feeding was given twice, in the morning and evening with the feeding rate of 10% per day of the total body weight. The research results showed that the addition of Binahong leaf powder did not influence the prevalence and survival rate of red tilapia seeds infected by Aeromonas hydrophila bacteria. Keywords: Aeromonas hydrophila, Bacteria, Binahong, Disease, Feed

scholarly journals Screening of some Indonesian medicinal plant extracts for anti quorum sensing activity to prevent Aeromonas hydrophila infection in Oreochromis niloticus

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Artini Pangastuti ◽  
Siti Lusi Arum Sari ◽  
Agung Budiharjo ◽  
SARI TRIYANA FITRI ◽  
PUJI SAYEKTI ◽  
...  
Keyword(s):  
Survival Rate ◽  
Quorum Sensing ◽  
Medicinal Plant ◽  
Plant Extracts ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Infected Fish ◽  
Medicinal Plant Extracts

Abstract. Pangastuti A, Sari SLA, Budiharjo A, Fitri ST, Sayekti P, Putri SR. 2021. Screening of some Indonesian medicinal plant extracts for anti quorum sensing activity to prevent Aeromonas hydrophila infection in Oreochromis niloticus. Biodiversitas 22: 3517-3522. Aeromonas hydrophila disease is a threat to Indonesia's aquaculture and fishing industries because of its results in mass deaths. Efforts to treat and prevent A. hydrophila infection in fish have so far been limited to the use of antibiotics. The use of these antibiotics can result in bacterial resistance as well as issues related to fish quality and environment. Another option is to target the bacterial quorum sensing (QS) system, a bacterial intercellular communication system that uses signal molecules, regulates the expression of A. hydrophila virulence factors such as exoprotease enzymes, elastase, and biofilms. The expression of A. hydrophila virulence factor can be inhibited by using QS inhibitor compounds, preventing infection from starting. The purpose of this study was to screen some Indonesian medicinal plant extracts for anti-QS activity and then use the selected extract to prevent A. hydrophila infection in Tilapia fish (Oreochromis niloticus). With the administration of extract, QS inhibition was observed in vitro based on the production of violacein in Chromobacterium violaceum; enzyme exoprotease, elastase, and the formation biofilm in A. hydrophila. Tilapia fishes were fed with extract supplementation and then infected with A. hydrophila in an in vivo infection challenge test. These test fishes were divided into six treatment groups given a different concentration of extract in feed (Healthy fish with a concentration of 0 g/100 g of feed as control; infected fish with a concentration of 0 g/100 g of feed; infected fish with a concentration of 0.2 g/100 g of feed; infected fish with a concentration 0.4 g/100 g of feed; infected fish with a concentration of 0.6 g/100 g of feed; infected fish with a concentration of 0.8 g/100 g of feed). Fish were reared for 2 weeks with the frequency of feeding 3 times a day. The variables observed included fish survival rate, behavior (swimming, agility, movement, and frequency of operculum opening), and morphology of the test fish. Data analysis of virulence factors production, fish survival rate, fish appetite, and frequency of operculum opening was carried out using the ANOVA variant test, continued with a 5% DMRT test. Others were analyzed descriptively. Of all the extracts studied, 4 extracts showed inhibition of the production of the violaceum pigment in C. violaceum. Muntingia calabura leaves extract was chosen for further testing due to its ease of availability and safety. The extract was significantly decreased the production of A. hydrophila caseinolytic and elastolytic protease, but had no effect on biofilm formation. In vivo challenge test with A. hydrophila showed better survival rate and fish condition in fish groups that were supplemented with M. calabura extract.

scholarly journals Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS)

2017 ◽  
Vol 16 (1) ◽  
pp. 92 ◽  
Author(s):  
, Sukenda ◽  
Kiki Amalia Pratiwi ◽  
, Rahman ◽  
Dendi Hidayatullah
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Passive Immunization ◽  
Relative Survival ◽  
Control Fish ◽  
Cell Vaccine ◽  
Relative Survival Rate ◽  
Whole Cell Vaccine ◽  
Antibody Titers ◽  
And Control

<p class="Pa3"><strong>ABSTRACT </strong></p><p> </p><p class="Pa5">Transfer of maternal immunity by mean of passive immunization is a way to provide protection and durability of antibodies on the offspring. The purpose of this research was to analize effication of <em>Aeromonas hydrophila </em>vaccine on the catfish broodstock to maternal transfer of immunity, and offspring resistance. The average body weight of broodstock used in this study were 650±50 g were kept in pool tarps sized 2×1×0.5 m3. This study used a randomized complete design with two treatments and three replications. Female broodstock were vaccinated using intraperitonial injections at a dose 0.4 mL/kg and control fish were injected with phospate buffered saline (PBS). The observed parameters include hematology of broodstock, mortality, the relative survival rate, and antibody titers. Antibody titer measurements on broodstock, eggs, and offspring. Vaccination on broodstock catfish delivers a significant antibody level (P&lt;0.05) on offspring compared to control catfish with relative survival rate of offspring at 5, 10, and 15 days after hatching were 67.76%, 82.66%, and 71.66% respectively.</p><p> </p><p class="Pa5">Keywords: catfish, <em>Aeromonas hydrophila</em>, vaccination, antibody transfer</p><p> </p><p> </p><p class="Pa3"><strong>ABSTRAK </strong></p><p> </p><p class="Pa5">Transfer kekebalan dari induk kepada benih melalui imunisasi pasif merupakan salah satu cara untuk memberikan proteksi pada benih. Tujuan penelitian ini untuk menguji efikasi vaksin sel utuh <em>Aeromonas hydrophila </em>pada induk ikan nila dalam mentransfer kekebalan spesifik ke benih dan menguji ketahanan benih hasil pemijahan induk yang divaksin. Induk lele yang digunakan pada penelitian ini memiliki bobot rata-rata 650±50 g dipelihara di kolam terpal berukuran 2×1×0,5 m3. Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Induk betina lele divaksinasi secara intraperitonial dengan dosis 0,4 mL/kg ikan dan induk lele kontrol disuntik dengan phospate buffer saline (PBS). Parameter yang diamati meliputi hematologi induk, mortalitas, tingkat kelangsungan hidup relatif benih, dan titer antibodi. Vaksinasi induk lele memberikan hasil level antibodi yang signifikan (P&lt;0,05) pada induk, telur, dan benih lele dibandingkan perlakuan kontrol dengan tingkat kelangsungan hidup relatif benih umur 5, 10, dan 15 hari pacatetas masing-masing sebesar 67,76; 82,66%; dan 71,66%.</p><p> </p><p>Kata kunci: ikan lele, <em>Aeromonas hydrophila</em>, vaksinasi, transfer antibodi</p>

scholarly journals The Elevated Expressions of Anti-lipopolysaccharide Factors After Priming Stimulation Confer Lastingly Humoral Protection in Crab Eriocheir sinensis

2021 ◽  
Vol 12 ◽  
Author(s):  
Weilin Wang ◽  
Yan Li ◽  
Siqi Fan ◽  
Xingye Lian ◽  
Wanqing Cao ◽  
...  
Keyword(s):  
Survival Rate ◽  
Recombinant Protein ◽  
Protective Effect ◽  
Aeromonas Hydrophila ◽  
Molecular Mechanisms ◽  
Eriocheir Sinensis ◽  
Immune Memory ◽  
Immune Protection ◽  
Immune Priming ◽  
Humoral Immune

Evidence of immune memory in invertebrates (immune priming) has accumulated in various organisms, and both cellular and humoral immune reactions are speculated to be involved in immune priming. However, there is a lack of understanding of the molecular mechanisms involved. In the present study, the protective effect of primed haemolymph was further validated by the increased survival rate of naïve crabs receiving a transfusion of primed haemolymph. By proteomic analysis, there were 474 proteins identified from the primed haemolymph, and most of them were functionally annotated in transport and metabolism classes. A total of 70 proteins were found to be differentially expressed in haemolymph at 12 hours and 7 days after priming stimulation with Aeromonas hydrophila, among which anti-lipopolysaccharide factor 1 (EsALF-1) and 3 (EsALF-3) were identified as the most significant (p &lt; 0.05). After being challenged with A. hydrophila, EsALF-1 and EsALF-3 were highly expressed at both mRNA (in haemocytes) and protein (in haemolymph) levels compared with blank crabs, and the mRNA expressions of components in the EsTLR1-EsMyd88-EsPelle-EsALF pathway also increased significantly (p &lt; 0.05). The EsALF-3 and EsMyd88 were even significantly higher expressed in response to the second A. hydrophila challenge, but their expressions all decreased (p &lt; 0.05) when EsTLR1 was knocked down by RNAi. After the naïve crabs received an injection with the recombinant protein of EsALF-1 (rEsALF-1) or EsALF-3 (rEsALF-3), their survival rate increased significantly (p &lt; 0.05) upon A. hydrophila stimulation. In contrast, the survival rate of the primed crabs reduced significantly (p &lt; 0.05) after they received an injection with the antibody of EsALF-1 or EsALF-3. The enhanced expressions of EsALF-1 and EsALF-3 after A. hydrophilap riming stimulation could sustain for four weeks. All the results suggested that the EsTLR1-mediated productions of EsALF-1 and EsALF-3 in haemolymph played an indispensable role in the month-long humoral immune protection induced by A. hydrophila, which provides solid evidence of immune priming in crabs and a valuable reference for further understanding immune memory in invertebrates.

scholarly journals The Effect of Potassium Diformate as Feed Additive on Immune Performances of Nilem (Osteochilus hasselti Valenciennes, 1842) Under Infection of Aeromonas hydrophila

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 11
Author(s):  
Ibnu Bangkit Bioshina Suryadi ◽  
Desty Nurul Ulfa ◽  
Ayi Yustiati ◽  
Rosidah Rosidah
Keyword(s):  
Water Quality ◽  
Survival Rate ◽  
Experimental Method ◽  
Aeromonas Hydrophila ◽  
Clinical Symptoms ◽  
The Body ◽  
Effective Dosage ◽  
Feed Additive ◽  
Random Design ◽  
Body Resistance

This research aims to determine the most effective dosage of potassium diformate additives in feed to increase the body resistance of nilem carp (Osteochilus hasselti). This research used Complete Random Design (CRD) experimental method with 4 treatments and 4 replications. The treatment used was the addition of potassium diformate on feed (0 %, 0.1 %, 0.3 % and 0.5 %). After 28 days of treatment, nilem fingerlings were challenged with Aeromonas hydrophila bacteria with a density of 108 cfu ml-1. Parameters observed were the number of leukocytes, erythrocytes, survival rate, clinical symptoms and water quality. The result showed that leukocytes and erythrocytes number were significantly different in each treatment. Meanwhile, leukocytes and erythrocytes number of nilem fingerlings in challenged test were increased and decreased due to the addition of potassium diformate and A. hydrophila infection. Clinical symptoms after challenged test were relatively similar such as haemorhagic, ulceration, exopthalmia, dropsy and abnormal swimming movements. Therefore, survival rate of nilem fingerlings with 0.3 % of potassiun diformate was the highest compared to other treatments, namely 81.67 %.

scholarly journals Pengaruh Cara Booster terhadap Efikasi Vaksinasi Oral dengan Debris Sel Aeromonas hydrophila pada Lele Dumbo (Clarias sp.)

2006 ◽  
Vol 8 (1) ◽  
pp. 36 ◽  
Author(s):  
Dini Siswani Mulia ◽  
Rachmansyah Rachmansyah ◽  
Triyanto Triyanto
Keyword(s):  
Survival Rate ◽  
Antibody Titer ◽  
Aeromonas Hydrophila ◽  
Challenge Test ◽  
Cell Debris ◽  
Oral Vaccination ◽  
Booster Injection ◽  
Time To Death ◽  
And Control ◽  
Mean Time

The aims of this research were to evaluate the effect of booster on the efficacy of vaccination with cell debris of Aeromonas hydrophila. Catfish (Clarias sp.) (10-13 cm of total length) were used for experiment with 4 treatments, i.e. (1) OS: oral vaccination and injection booster; (2) OO: oral vaccination and oral booster; (3) OR: oral vaccination and immersion booster; (4) without vaccination and booster (control). Booster was conducted one week after vaccination. The challenge test was conducted two weeks after booster. The results showed that the survival rate, Relative Percent Survival (RPS), Mean Time to Death (MTD), and antibody titer of OS treatment was significantly different (P<0.05) with OO, OR, and control. The survival rate and RPS of fish vaccinated by OS treatment reached 100%. The highest antibody titer was 2048 for OS treatment. The result sugested that oral vaccination followed by booster injection was the most effective method of vaccination.

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