scholarly journals Phytochemical analysis and antiproliferative properties of Pistacia atlantica leaves

2021 ◽  
Vol 11 (6-S) ◽  
pp. 21-25
Author(s):  
Fatiha Benahmed ◽  
Elazhari Mehrab ◽  
Omar Kharoubi
Keyword(s):  
Phenolic Compounds ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Mtt Assay ◽  
Human Tumor ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
Pistacia Atlantica ◽  
Kaempferol Glycoside

The medicinal plants may serve as potential sources for the development of new drugs and more effective anticancer agents for future therapy. Pistacia atlantica (Vahl.) Masters (Anacardiaceae) is an important plant used in traditional medicine practice in Algeria, and North Africa countries. The present study has the objective to estimate the in vitro antiproliferative (on the RD and Hep2 human tumor cell lines using the3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay) properties of the aqueous extracts of P. atlantica leaves, offering a phytochemical characterization of its aqueous extracts, by spectrophotometry methods. evealed by HPLC, phenolic compounds present among the five different flavonoids identified such as: ((epi)catechin, myricetin, quercetin and kaempferol glycoside derivatives). The MTT assay revealed that the tested extract had a good activity against Hep2 and RD cell lines with median inhibitory concentration (GI50)> 1000 μg/mL against Hep2 and (IC50) = 825,079 μg/mL against RD, (GI 50). The results showed a great bioactive potential for this species with a significant contribution of phenolic compounds, specially the flavonoids which makes it an interesting matrix in the development of novel pharmaceutical formulations. Planned future studies will involve the identification of different extract other than aqueous extract, determination of the mechanisms of action and the bioactive molecule of plant extracts. Keywords: Pistacia atlantica; aqueous extract; antiproliferative; MTT, HPLC.

scholarly journals In Vitro Interactions of Moroccan Propolis Phytochemical’s on Human Tumor Cell Lines and Anti-Inflammatory Properties

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 315 ◽  
Author(s):  
Soraia I. Falcão ◽  
Ricardo C. Calhelha ◽  
Soumaya Touzani ◽  
Badiaâ Lyoussi ◽  
Isabel C. F. R. Ferreira ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Tumor Cell ◽  
Cell Lines ◽  
Human Tumor ◽  
Inflammatory Activity ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Cell Lung Carcinoma ◽  
Human Tumor Cell Lines ◽  
Anti Inflammatory

Propolis is a resin manufactured by bees through the mixture of plant exudates and waxes with secreted substances from their metabolism, resulting in a complex mixture of natural substances of which quality depends on the phytogeographic and climatic conditions around the hive. The present study investigated the contribution of phenolic compounds to the cytotoxic and anti-inflammatory activities of propolis. The phenolic composition was evaluated by liquid chromatography with diode-array detection coupled to electrospray ionization tandem mass spectrometry (LC/DAD/ESI-MSn) analysis after phenolic extraction. The cytotoxicity of the extracts was checked using human tumor cell lines (MCF7- breast adenocarcinoma, NCI-H460- non-small cell lung carcinoma, HeLa- cervical carcinoma, HepG2- hepatocellular carcinoma, and MM127- malignant melanoma), as well as non-tumor cells (a porcine liver primary culture-PLP2). The anti-inflammatory activity was assessed using the murine macrophage (RAW 264.7) cell line. The results showed a composition rich in phenolic acids, such as caffeic and p-coumaric acid, as well as flavonoids, such as pinocembrin, pinobanksin, and pinobanksin-3-O-butyrate. Samples MP2 from Sefrou and MP3 from Moulay Yaâcoub presented a high concentration in phenolic compounds, while MP1 and MP4 from Boulemane and Immouzzer Mermoucha, respectively, showed similar composition with low bioactivity. The higher concentration of phenolic compound derivatives, which seems to be the most cytotoxic phenolic class, can explain the pronounced antitumor and anti-inflammatory activity observed for sample MP2.

In vitro antifilarial activity of extracts of the medicinal plant Cardiospermum halicacabum against Brugia pahangi

2000 ◽  
Vol 74 (3) ◽  
pp. 241-246 ◽  
Author(s):  
W. Khunkitti ◽  
Y. Fujimaki ◽  
Y. Aoki
Keyword(s):  
Medicinal Plant ◽  
Aqueous Extract ◽  
Mtt Assay ◽  
Ethanol Extract ◽  
Aqueous Extracts ◽  
Adult Males ◽  
Brugia Pahangi ◽  
Ethanol Extracts ◽  
Antifilarial Activity

AbstractThe in vitro effects of ethanol and aqueous extracts of the medicinal plant Cardiospermum halicacabum on adult worms and microfilariae of Brugia pahangi were investigated. With or without the plant extracts in culture medium, the motility of adult worms, microfilariae and microfilarial release from female worms were monitored daily. After 7 days of culture, viability or tissue damage of adult worms was assessed using the MTT assay. At > 500 μg ml-1, the aqueous extract significantly reduced motility of adult females after 24 h of exposure and adult males after 3 days. The aqueous extract, at > 500 μg ml-1, also significantly reduced microfilarial release from female worms, starting on day 2. The reduction in the motility of adult worms and the pattern of microfilarial release from female worms were concentration and time dependent. The MTT assay results revealed that adult worms cultured in the presence of aqueous extracts at > 500 μg ml-1 were damaged. However, the aqueous extract did not affect the motility of microfilariae with the exception of those in higher concentration extracts. Higher concentrations of ethanol extracts (2 mg ml-1) inhibited both the motility of adult worms and the release of microfilariae from females. Little effect of ethanol extracts was detected by the MTT assay, as only slight damage was caused to worms exposed only to the highest concentration (2 mg ml-1). However, ethanol extract at 500 μg ml-1 rapidly reduced the motility of microfilariae on day 2. The present study revealed that an aqueous extract of C. halicacabum has mild but definite direct macrofilaricidal action on B. pahangi.

scholarly journals Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

2016 ◽  
Vol 35 (6) ◽  
pp. 666-671 ◽  
Author(s):  
Adriana Campos ◽  
Débora Barbosa Vendramini-Costa ◽  
Giovanna Barbarini Longato ◽  
Tailyn Zermiani ◽  
Ana Lúcia Tasca Gois Ruiz ◽  
...  
Keyword(s):  
Cell Lines ◽  
Antiproliferative Activity ◽  
Human Tumor ◽  
Breast Cancer Cell Lines ◽  
Phytochemical Analysis ◽  
Human Tumor Cell Lines ◽  
Sulforhodamine B ◽  
Diterpene Ester ◽  
Sulforhodamine B Assay ◽  
Pure Compounds

Synadenium grantii is frequently used for the treatment of various diseases such as allergies, gastric disorders, and especially cancer. The aim of this study was to evaluate the possible antiproliferative potential of the methanol extract, fractions, and pure compounds from the stems of S grantii. Phytochemical analysis was carried out by conventional chromatographic techniques, and the antiproliferative activity was analyzed using the sulforhodamine B assay and an MTT-based assay. Nonpolar fraction and its subfractions from the stems of S grantii exhibited promising cytostatic effect against several human tumor cell lines (glioma, breast, kidney, and lung), with total grown inhibition values ranging from 0.37 to 2.9 μg/mL. One of the active principles of this plant was identified as a rare phorbol diterpene ester, denoted as 3,4,12,13-tetraacetylphorbol-20-phenylacetate. This compound demonstrated antiproliferative activity against glioma, kidney, lung, and triple-negative breast cancer cell lines. These results demonstrate that S grantii stems produce active principles with relevant antiproliferative potential.

scholarly journals Phytochemical analysis of therapeutic plants occurring in Dera Ismail Khan Region

2019 ◽  
Vol 2 (1) ◽  
Keyword(s):  
Amino Acids ◽  
Infectious Diseases ◽  
Punica Granatum ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
Sugar Amino Acids ◽  
Morus Nigra ◽  
Different Types ◽  
Pharmaceutical Industries

Plants contain different types of bio-active compounds that can be used to cure many diseases and also they can cure against many infectious diseases. These compounds are known as phytochemicals they may be primary or secondary constituents. Primary constituents have proteins, sugar, amino acids. Secondary constituents contain terpenoids, flavonoids and alkaloids. These compounds have antifungal as well as antibacterial activities.In this study different plants that are commonly found in Dera Ismail Khan region was investigated for phytochemical analysis, These plants were Acacia Nilotica, Luffa Cylindrical, Morus Nigra, Morus Alba, Punica Granatum and Psidium Guava commonly available in Dera Ismail khan region of Pakistan. Leaves, flower and fruits were washed, dried in air and ground to powdered. Aqueous extracts of leaves, flower and fruits were used for phytochemical analysis. All the parts were rich in phytochemicals i.e. terpenoids, tannins, flavonoids and steroids, etc. The phytochemical analysis has a great interest in pharmaceutical industries for new drugs and for curing different diseases. These phytochemicals found in this study can be used for curing different diseases.

scholarly journals Biosynthesis of Fe nanoparticles using Alhagi sparsifolia extract for the treatment of colorectal carcinoma in the in vitro condition: A pre-clinical trial study

2021 ◽  
Author(s):  
Yadong Zhou ◽  
Fafu Dou ◽  
Tahani Awad Alahmadi ◽  
Sulaiman Ali Alharbi ◽  
Milton Wainwright
Keyword(s):  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Mtt Assay ◽  
Iron Nanoparticles ◽  
Alhagi Sparsifolia ◽  
Leaf Aqueous Extract ◽  
Hct 116 ◽  
Human Colorectal Carcinoma ◽  
Ht 29

IntroductionThe preparation and formulation of new chemotherapeutic supplements and drugs with remarkable effects for the treatment of cancer are in the priority of both developing and developed countries. Recently, iron nanoparticles have been used as modern chemotherapeutic drugs for the treatment of several cancers such as leukemia, lung cancer, breast cancer, prostate cancer, etc. In the present study, iron nanoparticles were green-synthesized using the aqueous extract of Alhagi sparsifolia leaf aqueous extract.Material and methodsThe synthesized FeNPs were characterized by analytical techniques including SEM, TEM, UV-Vis., and FT-IR. The anti-human colorectal carcinoma activity of FeNPs was evaluated using MTT assay. In the cellular and molecular part of the recent study, the treated cells with FeNPs were assessed by MTT assay for 48h about the cytotoxicity and anti-human colorectal carcinoma properties on normal (HUVEC) and colorectal carcinoma cell lines i.e. HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10.ResultsThe nanoparticles were formed in a spherical shape in the average size of 47.24 nm. In the antioxidant test, the IC50 of FeNPs and BHT against DPPH free radicals were 161 and 134 µg/mL, respectively. The viability of malignant colorectal cell line reduced dose-dependently in the presence of FeNPs. The IC50 of FeNPs were 250, 293, 276, and 344 µg/mL against HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10 cell lines, respectively.ConclusionsAfter clinical study, iron nanoparticles containing Alhagi sparsifolia leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human colorectal carcinoma.

scholarly journals Phenolic Compounds and Bioactivity of Cytisus villosus Pourr.

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1994 ◽  
Author(s):  
Amel Bouziane ◽  
Boulanouar Bakchiche ◽  
Maria Dias ◽  
Lillian Barros ◽  
Isabel Ferreira ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungus ◽  
Antimicrobial Activities ◽  
Gram Positive ◽  
Aerial Parts ◽  
Ethyl Acetate Extracts

The present study focuses on the chemical composition, antioxidant, antimicrobial, and antiproliferative activities of the ethyl acetate and aqueous extracts obtained from the aerial parts of Cytisus villosus Pourr. HPLC-DAD-ESI/MSn was used to identify the phenolic compounds, being (epi)gallocatechin dimer the major compound (111 ± 5 µg/g·dw) in the aqueous extract, while myricetin-O-rhamnoside (226 ± 9 µg/g·dw) was the main molecule in the ethyl acetate extract. Both extracts exhibited good scavenging activities against DPPH radical (EC50 µg/mL of 59 ± 2 and 31 ± 2 for aqueous and ethyl acetate extracts, respectively). However, the ethyl acetate extract demonstrated more potent quenching activities than the aqueous extract. The antimicrobial activities were assessed on selected Gram-positive (Staphylococcus epidermidis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria, as well as on pathogenic fungus Candida glabrata. The extracts possessed selective and potent antimicrobial activities against the Gram-positive bacterium (IC50 of 186 ± 9 μg/mL and 92 ± 3 μg/mL for aqueous and ethyl acetate extracts, respectively). Finally, C. villosus extracts were evaluated for their antiproliferative potential on three human cancer cell lines representing breast and colon cancers. Although both extracts demonstrated sufficient growth inhibition of the three different cell lines, the ethyl acetate extract exhibited higher activity (LD50 values of 1.57 ± 0.06 mg/mL, 2.2 ± 0.1 mg/mL, and 3.2 ± 0.2 mg/mL for T47D, MCF-7, and HCT-116 cell lines). Both the extracts obtained from the aerial parts of C. villosus revealed very promising results and could be applied as functional agents in the food, pharmaceutical, and cosmeceutical industries.

scholarly journals Calendula officinalis green-mediated silver nanoparticles: Formulation, characterization and assessment of colorectal cancer activities green-mediated silver nanoparticles: Formulation, characterization and assessment of colorectal cancer activities

2021 ◽  
Author(s):  
Yongchao Xu ◽  
Behnam Mahdavi ◽  
Mohammad Zangeneh ◽  
Akram Zangeneh ◽  
Maryam Qorbani ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Silver Nanoparticles ◽  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Mtt Assay ◽  
Analytical Techniques ◽  
Spherical Shape ◽  
Calendula Officinalis ◽  
Human Colorectal Carcinoma

IntroductionThe biosynthesis of metal nanoparticles using medicinal plants is not only economical but also environmentally friendly as well as having miscellaneous biomedical applications. In the present study, silver nanoparticles were green-synthesized using the aqueous extract of Calendula officinalis.Material and methodsThe synthesized AgNPs@Calendula officinalis were characterized by analytical techniques including EDX, FE-SEM, XRD, UV-Vis., and FT-IR. The anti-human colorectal cancer activities of AgNPs@Calendula officinalis were evaluated using MTT assay.ResultsThe nanoparticles were formed in a spherical shape in the range of 38.05 to 75.41 nm for the particle size. On the other hand, the MTT assay was run to evaluate anti colorectal cancer activity of AgNPs@Calendula officinalis. In the cellular and molecular part of the recent study, the treated cells with AgNPs@Calendula officinalis were assessed by MTT assay for 48 h about the cytotoxicity and anti-human colorectal carcinoma properties on normal (HUVEC) and colorectal carcinoma cell lines i.e. WiDr, SW1417 [SW-1417], and DLD-1. In the antioxidant test, the IC50 of AgNPs@Calendula officinalis and BHT against DPPH free radicals were 222 and 124 µg/mL, respectively. The viability of malignant colorectal cell line reduced dose-dependently in the presence of AgNPs@Calendula officinalis. The IC50 of AgNPs@Calendula officinalis were 430, 326, and 392 µg/mL against WiDr, SW1417 [SW-1417], and DLD-1 cell lines, respectively.ConclusionsAfter the clinical study, silver nanoparticles containing Calendula officinalis leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human colorectal carcinoma.

scholarly journals Two-Dimensional PCA Highlights the Differentiated Antitumor and Antimicrobial Activity of Methanolic and Aqueous Extracts ofLaurus nobilisL. from Different Origins

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Maria Inês Dias ◽  
João C. M. Barreira ◽  
Ricardo C. Calhelha ◽  
Maria-João R. P. Queiroz ◽  
M. Beatriz P. P. Oliveira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Tumor Cell ◽  
Cell Lines ◽  
Human Tumor ◽  
Aqueous Extracts ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
Methanolic Extracts

Natural matrices are important sources of new antitumor and antimicrobial compounds. Species such asLaurus nobilisL. (laurel) might be used for this purpose, considering its medicinal properties. Herein,in vitroactivity against human tumor cell lines, bacteria, and fungi was evaluated in enriched phenolic extracts. Specifically, methanol and aqueous extracts of wild and cultivated samples ofL. nobiliswere compared considering different phenolic groups. Principal component analysis (PCA) was applied to understand how each extract acts differentially against specific bacteria, fungi, and selected human tumor cell lines. In general, the extract type induced the highest differences in bioactivity of laurel samples. However, from the PCA biplot, it became clear that wild laurel samples were higher inhibitors of tumor cell lines (HeLa, MCF7, NCI-H460, and HCT15). HepG2 had the same response to laurel from wild and cultivated origin. It was also observed that methanolic extracts tended to have higher antimicrobial activity, except againstA. niger, A. fumigatus, andP. verrucosum. The differences in bioactivity might be related to the higher phenolic contents in methanolic extracts. These results allow selecting the extract type and/or origin with highest antibacterial, antifungal, and antitumor activity.

scholarly journals In vitro oocysticidal sporulation inhibition of Eimeria tenella and antioxidant efficacy of ethanolic and aqueous extracts of Conyza aegyptiaca

2021 ◽  
Vol 6 (1) ◽  
pp. 30-40
Author(s):  
Emmanuel Tana Toah ◽  
◽  
Vincent Khan Payne1, ◽  
Yamssi Cedric ◽  
Noumedem Anangmo Christelle Nadia ◽  
...  
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Hot Water ◽  
Phytochemical Analysis ◽  
Aqueous Extracts

Avian coccidiosis is probably one of the most expensive parasitic diseases with major economic impact on poultry industries worldwide. The purpose of this study was to evaluate the ethanolic and aqueous extracts of Conyza aegyptiaca in terms of phytonutrients, in vitro oocysts sporulation inhibition and antioxidant properties. The extraction process of plant leaf powder (100 g) pulverized using a clean manual grinder was carried out in ethanol and hot water and the yields were calculated as a percentage ratio of extract mass on plant powder mass after solvent evaporation. Phytochemical analysis procedures were performed to determine the presence of phytonutrients. The in vitro oocysticidal sporulation inhibition was determined at five different concentrations (0.25; 0.5; 1; 2 and 4 mg/ml) of each extract in petri dishes each containing 3000 unsporulated oocysts and examined after 24 and 48 hours under a microscope. In vitro antioxidant capacity of extracts was estimated using different assays. Quantitative aqueous extract (11.72%) was higher than ethanolic extract (4.34%). In terms of qualitative yields, ethanolic extract revealed higher phytonutrients investigated (100%) than aqueous extract (42.86%). The sporulation inhibition of ethanolic extract was generally higher than the aqueous extract after 24 and 48 h and varied according to the different tested concentrations. In all the antioxidant assays, ethanolic extract exhibited significant free radical scavenging activity with inhibitory concentration (IC50=26.10±1.09) close to that of ascorbic acid at the probability level of 5% error (p<0.05). The ethanolic extract with higher free radical scavenging activities and ferric reducing effect also showed significant higher content of both phenols (127.01±3.99 mgGAE/g) and flavonoids (108.66±3.49 mgCE/g) than aqueous extracts, suggesting correlation between phenolic content and antioxidant activity. Data from this study could be used for developing bioactive elements for natural anticoccidials and antioxidants of health promoting activities

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