ANTIOXIDANT ACTIVITY OF ETHANOLIC EXTRACT OF OCIMUM KILIMANDSCHARICUM USING HYDROXYL RADICAL SCAVENGING METHOD

The aim of this study was to estimate the content of polyphenols, flavonoids and tannins and to evaluate the antioxidant property and the antiulcer activity of the ethanolic extract of melon (Cucumis melo L. var. inodorus) pulp on ethanol-induced gastric ulcers in rats. Ferric reducing power and hydroxyl radical scavenging tests were applied to evaluate the in vitro antioxidant activity. The polyphenolic and flavonoids contents of melon extract were found to be 56.5 ± 2.49 mg GAE/g of dry extract and 0.43 ± 0.09 mg QE/ g of dry extract, respectively. Whereas the tannins content was 48. 30 ± 0. 9 mg ETA/ g of dry extract. Melon pulp extract exhibited a good reducing potential with an EC50 of 4.23 ± 0.08 mg/ml and high hydroxyl radical scavenging activity with IC50 of 1.83± 0.09 mg/ml. Oral administration of the melon pulp extract at doses of 200 and 600 mg/kg to rats reduced gastric mucosal lesions dose-dependently with percentage protection of 56.56 and 93.79% compared to omeprazole (95.92 %) as reference drug. These results showed that the melon pulp extract had a good antioxidant activity and gastroprotective potential suggesting it use as an adjuvant in the treatment of gastric ulcer. Keywords: Cucumis melo var. inodorus, Polyphenols, Flavonoids, Tannins, Ulcer, Rat.

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Antioxidant activity of ethanolic extract of Penicillium chrysogenum and Penicillium fumiculosum

Hemijska industrija ◽

10.2298/hemind121102027j ◽

2014 ◽

Vol 68 (1) ◽

pp. 43-49 ◽

Cited By ~ 7

Author(s):

Violeta Jakovljevic ◽

Jasmina Milicevic ◽

Jelica Stojanovic ◽

Slavica Solujic ◽

Miroslav Vrvic

Keyword(s):

Antioxidant Activity ◽

Penicillium Chrysogenum ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Ethanolic Extract ◽

Free Radical Scavenging ◽

Scavenging Activity ◽

Total Antioxidant ◽

Chelating Ability

The aim of this study was to investigate the biological and chemical activity on two species of fungi of the genus Penicillium isolated from wastewater. On the selected species of fungi the different antioxidant activity assays were carried out: DPPH free-radical scavenging activity, total antioxidant activity, Fe2+- chelating ability and Fe3+- reducing power. Total phenol content was also determinate for ethanolic extract of mycelia. Penicillium chrysogenum ethanolic extract contained higher total phenolic content and better total antioxidant capacity as well as ferrous ion chelating ability. Penicillium fumiculosum ethanolic extract showed higher DPPH free-radical scavenging activity, as well as reducing power. Based on the obtained results it can be concluded that two types of fungi are potential new sources of natural antioxidants.

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Antioxidant Activities of Extracts from Celery Leaves (Apium Graveolens L) Grown in Jos, Nigeria

International Research Journal of Pure and Applied Chemistry ◽

10.9734/irjpac/2020/v21i430160 ◽

2020 ◽

pp. 1-5

Author(s):

M. Suleman Stephen ◽

E. A. Adelakun ◽

J. H. Kanus ◽

Meshack M. Gideon

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Acetone Extract ◽

Apium Graveolens ◽

Isolation And Identification ◽

Celery Plant

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.

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Evaluation antioxidant activity and corrosion inhibition of C38 in Hydrochloric acid medium by dried lemon peels of Kenitra Marrakech cities in Morocco and Taiz town in Yemen: A Comparative study

SHS Web of Conferences ◽

10.1051/shsconf/202111904001 ◽

2021 ◽

Vol 119 ◽

pp. 04001

Author(s):

Khaled Abdu ◽

Rahma Erahioui ◽

Khadija Khedid ◽

Hefdhal deen ◽

Maha Elhawary ◽

...

Keyword(s):

Antioxidant Activity ◽

Comparative Study ◽

Corrosion Inhibition ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Fatty Tissue ◽

Scavenging Activity ◽

Ic50 Values

This paper presents a comparative study about the evaluation of antioxidant activity and corrosion inhibition in Kenitra, Marrakesh and Taiz. An interesting topic, indeed polyphenols can improve or help to reduce oxidative stress in the treatment of digestive problems, weight management difficulties, diabetes, hypertension, arteriosclerosis and damage fatty tissue. Therefore, the authors deal with the measurement of polyphenol content and evaluation of the antioxidant activity of lemon peels in Kenitra , Marrakesh and Taiz.The authors performs empirical analyzes on lemon peels. The total polyphenol contents of the ethanolic extract of lemon peels were measured. It was to be 30. 23, 26. 346 and 20.961 mg/CE/g in Kenitra, Marrakech, and Taiz, respectively. Moreover, the DPPH radical scavenging activity of ethanolic extract of dried lemon peels was higher than 200μg/ml concentration. They were 73.47%, 47.36%, and 32.09in Kenitra, Marrakech, and Taiz, respectively. Also, the IC50 values of ethanolic extracts calculated from the percentage inhibitions at the same concentration. Inhibition (IC50) which obtained in Kenitra was 123.089 μg/ml. It was lower compared to the ethanol extract of Marrakech and Taiz that were 197.418, 276.750μg/mL, respectively. Therefore, the extract which is containing a high amount of phenolic is showed high radical scavenging activity. In addition, the maximum inhibition efficiencies for 2 mL L-1 of the inhibitor at 298 k were 98, 12% and 84, 85 % in Kenitra and Taiz, respectively. These values obtained through polarization curve measurements.

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Evaluation of antioxidant activity of Melothria maderaspatana in vitro

Open Life Sciences ◽

10.2478/s11535-010-0005-5 ◽

2010 ◽

Vol 5 (2) ◽

pp. 224-230 ◽

Cited By ~ 7

Author(s):

Boobalan Raja ◽

Kodukkur Pugalendi

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Free Radical ◽

Hydroxyl Radical ◽

Superoxide Anion ◽

Radical Scavenging ◽

Reducing Power ◽

Free Radical Scavenging ◽

Melothria Maderaspatana

AbstractIn this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentrations of leaf extract exhibited free radical scavenging and antioxidant power, and the preventive effects were in a dose-dependent manner. The antioxidant activities of the above were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and α-tocopherol. The results obtained in the present study indicate that the M. maderaspatana extract could be considered a potential source of natural antioxidant.

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IN VITRO ANTIOXIDANT ACTIVITY AND GC-MS ANALYSIS OF THE ETHANOLIC EXTRACTS OF TERMINALIA BELLERICA ROXB (BAHEDA)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i11.14175 ◽

2016 ◽

Vol 8 (11) ◽

pp. 275

Author(s):

Rasna Gupta ◽

Ram Lakhan Singh ◽

Neeraja Dwivedi

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Total Phenolic ◽

Scavenging Activity ◽

Terminalia Bellerica ◽

Ms Analysis ◽

Phytochemical Constituents

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.

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ANTIOXIDANT ACTIVITY AND CARDIOPROTECTIVE ACTIVITY OF BANGUN-BANGUN LEAVES (PLECTRANTHUS AMBOINICUS LOUR.) ETHANOLIC EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i9.26907 ◽

2018 ◽

Vol 11 (9) ◽

pp. 165

Author(s):

Modesta Harmoni Tarigan ◽

Urip Harahap ◽

Aminah Dalimunthe ◽

Nerdy Nerdy

Keyword(s):

Antioxidant Activity ◽

Troponin T ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Heart Tissue ◽

Positive Control ◽

Control Group ◽

Activity Test ◽

Ic50 Value ◽

Plectranthus Amboinicus

Objectives: The objectives of this study were to determine the antioxidant activity and cardioprotective activity of bangun-bangun leaves ethanolic extract.Methods: Bangun-bangun leaves ethanolic extract was obtained by maceration process. The antioxidant activity test was performed by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging method with various concentrations of extract. The absorbance was measured by visible spectrophotometric method and calculated the inhibitory concentration (IC50) value for antioxidant activity analysis. Cardioprotective activity test was performed by measuring the cardiac troponin T (cTnT) level, creatine kinase-muscle/brain (CK-MB) level, and histology of the heart tissue. Animals induced with doxorubicin at the 8th day and the 9th day, bangun-bangun leaves ethanolic extract was administered from the 1st day to the 9th day with various doses of extract.Results: Bangun-bangun leaves ethanolic extract had IC50 value of 57.79 μg/mL. Difference dose of bangun-bangun leaves ethanolic extract shows difference cardioprotective activity. Bangun-bangun leaves ethanolic extract at dose 300 mg/kg bw did not differ significantly to the positive control group and normal group. The higher the dose of an extract the greater the decrease in cTnT and CK-MB levels and increase protection against heart damage.Conclusion: Bangun-bangun leaves ethanolic extract had strong antioxidant and had cardioprotective activity.

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Antioxidant and Hypoglycemic Properties of Seeds of Cucurbitaceae Family from Western Nepal

Journal of Health and Allied Sciences ◽

10.37107/jhas.118 ◽

2019 ◽

Vol 9 (2) ◽

pp. 1-5

Author(s):

Atisammodavardhana Kaundinnyayana ◽

Anita G.C ◽

Asmita Banstola ◽

Kishwor Poudel ◽

Nirmal Acharya ◽

...

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Positive Control ◽

Lagenaria Siceraria ◽

Benincasa Hispida ◽

Ic50 Values ◽

In Vitro Antioxidant Activity

Introduction: Diabetes mellitus is an endocrine and metabolic disorder characterized by the defect in insulin secretion or insulin action or both. The association between diabetes mellitus and oxidative stress is well established. Cucurbitaceae is the largest family containing 120 genera mostly grown for its sweet and juicy fruit in warm climates all over the world. In the present work ethanolic extracts of the seeds of Trichosanthes cucumerina Linn (Chichinno), Lagenaria siceraria (Lauka), Cucurbita pepo (Pharsi), Luffa aegiptiaca (Ghiraula), and Benincasa hispida (Kubindo) collected from Western Nepal has been evaluated for their in vitro antioxidant activity and in vivo hypoglycemic effects. Methods: In vitro antioxidant activity was assessed by using DPPH free radical scavenging activities and their IC50 values were calculated. In vivo hypoglycemic effect was examined on normoglycemic rats. The clinical significance of ethanolic extract at the doses of 250 and 500 mg/kg body weight was investigated in 0, 30, 60, 120 and 180 minutes of oral administration. Metformin treated group was used as the positive control. Results: For DPPH radical scavenging action, the IC50 values of Trichosanthes cucumerina, Luffa aegiptiaca, Benincasa hispida, Cucurbita pepo, Lagenaria siceraria were found to be 60.72, 127.73, 49.63, 98.16 and 52.46 µg/ml respectively which were compared to the IC50 value of ascorbic acid (Positive control) which was found to be 38.11 µg/ml. The extracts of Benincasa hispida, and were having higher antioxidant activity and tested for in vivo hypoglycemic activity.In vivo administration of two doses of ethanolic extract of Lagenaria siceraria reduced the level of blood glucose while the best result was obtained at 250 mg/kg. Conclusions: Present study revealed promising antioxidant and hypoglycemic activity of ethanolic extract of Lagenaria siceraria. The further exploration of Lagenaria siceraria for its effective use in the traditional medicinal system is essential.

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Composition, color and antioxidant properties of cocoa shell at different roasting temperatures

Food Research ◽

10.26656/fr.2017.4(3).251 ◽

2019 ◽

Vol 4 (3) ◽

pp. 585-593

Author(s):

R. Fakhlaei ◽

Rozzamri A. ◽

N. Hussain

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Natural Antioxidants ◽

Food Ingredient ◽

Cocoa Products ◽

Cocoa Shell ◽

L Value

Cocoa shell is underexploited and is considered as waste. However, roasting cocoa is a fundamental step in producing cocoa products. Composition of the cocoa shell depends on the origin whereas cocoa processing involves fermentation, drying and roasting. However, scarce research has been conducted to analyze the composition of Malaysian cocoa shells at different roasting temperatures. Therefore, this research aims to determine the effect of different roasting temperatures (110, 120, 130, 140 and 150°C) on the proximate composition, colour and antioxidant activity of cocoa shell. Changes in antioxidant activity and polyphenol content were recorded. Fiber and moisture content were significantly (p<0.05) higher in unroasted cocoa shell. However, the protein was found to decrease significantly (p<0.05) after roasting at 150°C (10.93%). Fat content was found higher in roasted cocoa shell at 150°C (6.82%) compared to the unroasted cocoa shell (6.09%). The colour (L value) recorded significantly (p<0.05). the highest for unroasted cocoa shell (51.28) and the lowest at 150°C (47.93), DPPH radical scavenging activity of ethanolic extract of cocoa shell was significantly (p<0.05) higher in 0% ethanol concentration compared to other ethanol concentrations (20, 50, 80 and 100%). The roasted cocoa shell may be considered as a source of natural antioxidants for functional food development and intermediate food ingredient.

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Hepatoprotective and Antioxidant Activity of Lannea coromandelica Linn. on Thioacetamide Induced Hepatotoxicity in Rats

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.8.30 ◽

2014 ◽

Vol 8 ◽

pp. 30-43 ◽

Cited By ~ 3

Author(s):

V. Srinivasa Rao ◽

John Wilking Einstein ◽

Kuntal Das

Keyword(s):

Antioxidant Activity ◽

Serum Concentration ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Bark Extract ◽

High Dose ◽

Lannea Coromandelica ◽

Male Wistar Rats

Hepatoprotective and antioxidant activity of Lannea coromandelica bark extract (LCBE) was investigated on thioacetamide induced hepatotoxicity in rats. Hepatotoxicity was induced by thioacetamide (TAA) administration (100 mg/kg. s.c). LCBE at different doses (400 and 200 mg/kg) were administered orally to male wistar rats. Thioacetamide caused elevation of serum concentration of AST, ALT, ALP, serum bilirubin and also reduced serum concentration of total protein, albumin, sodium, potassium in animals as compared to control (p < 0.05) but LCBE treated rats showed maximum reduction of AST [(138±5.1) IU/L], ALT [(71 ±2.7) IU/L], ALP [(140 ±1.9) IU/L] with the high dose (400 mg/kg bw) of combined aqueous and alcoholic bark extract. Whereas, serum bilurubin, cholesterol, sugar and LDH content were varied with the treatments but showed higher with the only ethanolic extract at dose of 400 mg/kg. The IC50 value was observed as (83.28 ±2.12) µg/mL, for DPPH radical scavenging activity. Result concluded that ethanolic extract and combined aqueous and alcoholic bark extract of L. coromandelica showed a potential hepatoprotective and antioxidant activities might be due to the presence of phenolic groups, terpenoids and alkaloids.

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