scholarly journals Biotesting of cultivation zoocompost of Hermetia illucens larvae

2020 ◽  
Vol 28 (4) ◽  
pp. 324-335
Author(s):  
Elena N. Goncharova ◽  
Ivan R. Kurzenev ◽  
Marina I. Vasilenko ◽  
Evgeniy A. Pendyurin
Keyword(s):  
Daphnia Magna ◽  
Organic Compounds ◽  
Chlorella Vulgaris ◽  
Avena Sativa ◽  
Protein Production ◽  
Hazard Class ◽  
Hermetia Illucens ◽  
Triticum Vulgare ◽  
Test Organisms ◽  
Hermetia Illucens Larvae

A by-product of protein production from the larvae of the black soldier fly ( Hermetia illucens ) is a zoocompost consisting of a variety of organic compounds. The purpose of the work was to determine the hazard class of the zoo complex - the waste of cultivation of Hermetia illucens larvae using biotesting methods, since the use of calculated methods to determine the hazard class is difficult. Biotesting experiments were carried out on test organisms: planktonic crustacean Daphnia magna , algae Chlorella vulgaris , seeds of Avena sativa , Triticum vulgare and Hordeum sativum . It has been established that the zoocompost refers to low-risk waste. Extractions of the zoocompost stimulate the growth of plants Avena sativa and Hordeum sativum , it is supposed to be used as a phytostimulator. It has been shown that an environmentally friendly wasteless method for the production of protein additive from insects is possible.

A Standard Primary Toxicity Bioassay Using Daphnia Magna as a Test Species

1977 ◽  
Vol 12 (1) ◽  
pp. 27-50 ◽  
Author(s):  
L.A. Behie ◽  
J.E. Zajic ◽  
D. Berk ◽  
R.J.P. Brouzes ◽  
V.A. Naish
Keyword(s):  
Statistical Analysis ◽  
Daphnia Magna ◽  
Biological Indicator ◽  
Pulp And Paper ◽  
Toxicity Bioassay ◽  
Simple Method ◽  
Test Species ◽  
Test Organisms ◽  
Dilution Water

Abstract Although Daphnia magna have been widely used in the determination of the toxicity of various substances, there are no reports in the literature that describe a rigorous bioassay method using this organism as a test species. The test described herein involves the standariza-tion of various important aspects of the method such as the age of the test organisms, and the dilution water used for the preparation of the various toxicant concentrations. Also described is a simple method for the statistical analysis of the results. The sensitivity of the proposed bioassay is demonstrated by determining the toxicity of various pulp and paper effluents. Finally, extensive bioassays were carried out simultaneously with rainbow trout and Daphnia magna indicating that Daphnia are as good a biological indicator of acute toxicity as fish.

Effect of Mercury on the Survival of Daphnia Magna

1990 ◽  
Vol 22 (5) ◽  
pp. 241-246 ◽  
Author(s):  
I. Brković-Popović
Keyword(s):  
Life Span ◽  
Daphnia Magna ◽  
Abiotic Factors ◽  
Total Hardness ◽  
Chemical Characteristics ◽  
Effect Concentration ◽  
Test Organisms ◽  
Lethal Time ◽  
Two Temperatures ◽  
Time Required

The median lethal time (LT) for each concentration of mercury was determined on the basis of mortality curves. Toxicity curves (i.e., the median life-span of Daphniamagna, LT 50, versus the concentration of mercury) for four tested conditions are shown. The experiments were carried out using two diluents of different total hardness (46 and 119 mg/l as CaCO3), at two temperatures (20°C and 25°C). A comparison of the LT 50s of the control organisms with the LT 50s of the test organisms at mercury concentrations of 0.0075 and 0.0050 mg. l-1 showed that the chemical characteristics and temperatures of the mediums tested did not affect the range of the ‘no effect' concentration. However, the incipient LC 50, and the time required for its appearance, did depend on the combination of the abiotic factors tested.

scholarly journals Valorisation of Organic Waste By-Products Using Black Soldier Fly (Hermetia illucens) as a Bio-Convertor

2021 ◽  
Vol 13 (15) ◽  
pp. 8345
Author(s):  
Kieran Magee ◽  
Joe Halstead ◽  
Richard Small ◽  
Iain Young
Keyword(s):  
Food Waste ◽  
Fish Feed ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
High Quality Protein ◽  
The Face ◽  
Beet Pulp ◽  
By Products ◽  
Hermetia Illucens Larvae

One third of food produced globally is wasted. Disposal of this waste is costly and is an example of poor resource management in the face of elevated environmental concerns and increasing food demand. Providing this waste as feedstock for black soldier fly (Hermetia illucens) larvae (BSFL) has the potential for bio-conversion and valorisation by production of useful feed materials and fertilisers. We raised BSFL under optimal conditions (28 °C and 70% relative humidity) on seven UK pre-consumer food waste-stream materials: fish trimmings, sugar-beet pulp, bakery waste, fruit and vegetable waste, cheese waste, fish feed waste and brewer’s grains and yeast. The nutritional quality of the resulting BSFL meals and frass fertiliser were then analysed. In all cases, the volume of waste was reduced (37–79%) and meals containing high quality protein and lipid sources (44.1 ± 4.57% and 35.4 ± 4.12%, respectively) and frass with an NPK of 4.9-2.6-1.7 were produced. This shows the potential value of BSFL as a bio-convertor for the effective management of food waste.

The effect of dietary full-fat Hermetia illucens larvae meal on gut physiology and growth performance in young turkeys

2021 ◽  
Vol 275 ◽  
pp. 114879
Author(s):  
Jan Jankowski ◽  
Krzysztof Kozłowski ◽  
Zenon Zduńczyk ◽  
Anna Stępniowska ◽  
Katarzyna Ognik ◽  
...  
Keyword(s):  
Growth Performance ◽  
Hermetia Illucens ◽  
Gut Physiology ◽  
Hermetia Illucens Larvae

scholarly journals Conversion of Spent Coffee and Donuts by Black Soldier Fly (Hermetia illucens) Larvae into Potential Resources for Animal and Plant Farming

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 332
Author(s):  
Hayden Fischer ◽  
Nicholas Romano ◽  
Amit Kumar Sinha
Keyword(s):  
Organic Waste ◽  
Glycogen Content ◽  
Organic Fertilizer ◽  
Organic Fertilizers ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Hermetia Illucens Larvae ◽  
Nitrogen Phosphorus ◽  
Dough Stage ◽  
Plant Farming

Nutritionally unbalanced organic waste can be converted into potential resources for animal and plant farming by culturing black soldier fly (Hermetia illucens) larvae (BSFL) and prepupae (BSFP). BSFL and BSFP are rich sources of protein and lipids, while the leftover excrement called “frass” can be used as an organic fertilizer. Using readily available resources, BSFL were cultured on spent coffee, donut dough or an equal blend for 35 days. Survival, productivity, daily pupation and biochemical composition of BSFL and BSFP were measured along with the nitrogen-phosphorus-potassium values of the frass. Survival was highest in the blend compared (81%) to spent coffee (45%) or dough (24%); however, BSFL and BSFP were significantly longer and heavier from dough. Stage and food significantly influenced the protein, lipid and glycogen content of the BSFL and BSFP, which tended to be higher in the latter. While fatty acids were often significantly higher in BSFL fed spent coffee, the amino acid composition of BSFL was generally higher in dough. Frass from the blend had significantly highest nitrogen content, while potassium and phosphorus were significantly higher and lower from spent coffee, respectively. Although coffee and donut dough were suboptimal substrates for BSFL, a blend of these produced BSFL and frass that were nutritionally comparable to soybean meal and many organic fertilizers, respectively.

Investigating the process of electrocoagulation in the removal of azo dye from synthetic textile effluents and the effects of acute toxicity on Daphnia magna test organisms

2022 ◽  
Vol 45 ◽  
pp. 102485
Author(s):  
Carlos Eduardo Lach ◽  
Camila Schwarz Pauli ◽  
Aline Scheller Coan ◽  
Edesio Luiz Simionatto ◽  
Luciano André Deitos Koslowski
Keyword(s):  
Acute Toxicity ◽  
Daphnia Magna ◽  
Azo Dye ◽  
Textile Effluents ◽  
Test Organisms

A simple and rapid protocol for measuring the chitin content of Hermetia illucens (L.) (Diptera: Stratiomyidae) larvae

2020 ◽  
Vol 6 (3) ◽  
pp. 285-290 ◽  
Author(s):  
M.J. Woods ◽  
N.J. Goosen ◽  
L.C. Hoffman ◽  
E. Pieterse
Keyword(s):  
Nitrogen Content ◽  
Standard Error ◽  
Inorganic Compounds ◽  
Insect Larvae ◽  
Hermetia Illucens ◽  
Chitin Content ◽  
Chloroform Methanol ◽  
Hermetia Illucens Larvae ◽  
Inorganic Components

The study reports on a simple gravimetrical analysis to determine the chitin content of insect larvae. Hermetia illucens larvae, 16 days of age, was used as sample material. The method of analysis comprised of a defatting treatment by means of rapid solvent extraction (2:1 chloroform : methanol), followed by a treatment with 1 M HCL (demineralisation) and 1 M NaOH (deproteinisation). The nitrogen content of the obtained chitin was determined and compared to that of the nitrogen content of pure chitin (6.89). The chitin content of H. illucens larvae was determined to be 5.68±0.15% with a nitrogen content of 6.43±0.038 (mean ± standard error). The average nitrogen content of the isolated chitin was lower than the theoretical value calculated for pure chitin. This indicated that there was still a small amount of inorganic compounds present in the chitin of the insect larvae after applying the developed analytical procedures. This was confirmed by the ash value of the isolated chitin (1.50±0.06%) (mean ± standard error). The analysis is simple and accurate, which gives repeatable results, for the determination of the chitin content of H. illucens larvae. Further studies regarding the demineralisation treatment could improve the accuracy of the method due to the removal of all inorganic components. Future studies could also investigate the accuracy of the protocol on other insect species.

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