Radical Scavenging, Preliminary Phytochemical Screening and DNA Protective Effect of Carissa Carandas Linn Plant Leaves

The objective of this study was to evaluate the phytochemical and pharmacological screening of fruit extracts of Aegle marmelos using various in vitro methods. The antioxidant activity of the extracts was evaluated by using DPPH free radical scavenging and reducing power method, while well diffusion method was used for antimicrobial activity. Phytochemical screening indicated the presence of flavonoids, and phenolics were abundantly present in methanolic and aqueous extracts of A. marmelos. Methanolic extract showed significant antioxidant potential, which was however inferior to that of ascorbic acid. The methanolic extract showed maximum zone of inhibition against the S. aureus (18 mm) and it was higher than that of the standard tetracycline. The HPLC data predict that the presence of scopoletin, marmelosin and umbelliferone, which may contribute to their pharmacological action.

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FINGER PRINTING ANALYSIS OF THE PHENOLS FROM HOLOPTELEA INTEGRIFOLIA (ROXB.) PLANT LEAVES USING HPTLC

INDIAN DRUGS ◽

10.53879/id.54.09.10727 ◽

2017 ◽

Vol 54 (09) ◽

pp. 67-71

Author(s):

R. C. Sutar ◽

◽

D. S Musmade

Keyword(s):

High Performance ◽

Methanolic Extract ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Plant Leaves ◽

Methanol Extracts ◽

Finger Printing ◽

Rf Values ◽

Layer Chromatography ◽

Tungsten Lamp

The present study was conducted to identify the phenols from methanol extracts (MHI) of medicinally and economically useful leaves of Holoptelea integrifolia (Roxb.) plant using High Performance Thin Layer Chromatography (HPLC) technique. Preliminary phytochemical screening was done and HPTLC studies were carried out on CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp. Preliminary phytochemical screening of methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins, phenols and carbohydrates. HPT LC finger printing of phenols of methanolic extract of leaf revealed seven polyvalent phytoconstituents (7 peaks) and corresponding ascending order of Rf values in the range of 0.15 to 0.75. From the results of preliminary phytochemical analysis and above Rf values, we have concluded the presence of phenols in methanol extracts.

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Determination of in Vivo Biological Activities of Dodonaea Viscosa Flowers Against CCL4 Toxicity in Albino Mice With Bioactive Compound Detection

10.21203/rs.3.rs-275991/v1 ◽

2021 ◽

Author(s):

Hina Gul ◽

Muhammad Awais ◽

Salina Saddick ◽

Falak Sher Khan ◽

Muhammad Gulfraz ◽

...

Keyword(s):

Protective Effect ◽

Biological Activities ◽

Hematological Parameters ◽

Octadecenoic Acid ◽

Radical Scavenging ◽

Herbal Medicines ◽

Bioactive Compound ◽

Dodonaea Viscosa

Abstract Dodonaea viscosa L. Jacq. is an evergreen shrub and native to Asia, Africa and Australia. It has been used as traditional medicine in different countries. The foremost objective of the current study was to discover protective potential of D. Viscosa flowers Methanol (DVM) and Chloroform (DVC) extracts against CCL4 induced toxicity in mice. This study was intended to identify phytochemicals through HPLC, GCMS and FT-IR as well as in vitro antioxidant and in vitro antituberculosis activity. Our comprehensive findings indicate that Dodonaea viscosa is valuable and widespread herbal medicines through therapeutic potentials for curing various ailments. Dodonaea viscosa flowers are found to have protective effect against oxidative stress produced by CCL4 in liver, kidney and spleen. The level of hepatic enzymes (ALP, AST ALT and Direct bilirubin), hematological parameters (RBCs, WBCs and Platelets), total protein and liver antioxidant enzymes (SOD, GPx and CAT) were restored by the intake of DV extracts after decline in levels by CCL4. Histopathological results discovered the defensive effect of 300mg/kg of DVM extract against CCL4 induced damage, thus having improved protective effect as compared to DVC and control. As a result of analysis total flavonoids and total phenolics were also revealed. Phytochemical investigation by HPLC identified gallic acid, epicatechin, cumeric acid, flavonoids while Oleic acid (Octadecenoic acid) (C18H34O2), Stearic acid (C18H36O2), Ricinoleic acid (C18H34O3) and Cedrol (C15H26O) was estimated by GCMS. DVM extract exhibited resistance against in vitro Mycobacterium tuberculosis strains. This study proposed that protective effect of DV against oxidative damage induced in Liver, Kidney and Spleen can possibly be correlated to their antioxidant as well as free radical scavenging property.

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Pharmacological studies of Mangifera indica leaf extract

World Journal of Biology Pharmacy and Health Sciences ◽

10.30574/wjbphs.2021.7.3.0100 ◽

2021 ◽

Vol 7 (3) ◽

pp. 073-079

Author(s):

Agrawal RC

Keyword(s):

Hydroxyl Radical ◽

Radical Scavenging Activity ◽

Mangifera Indica ◽

Radical Scavenging ◽

Antibacterial Activities ◽

Positive Control ◽

Screening Methods ◽

Phytochemical Screening ◽

Test Organisms ◽

Almost All

The present study was undertaken to explore the phytochemical screening, anti-bacterial and anti-oxidant activities of the hydro-methanolic leaves extract of Mangifera indica using standard screening methods such as disc diffusion and DPPH methods. In phytochemical screening, Mangifera indicaextract showed presence of secondary metabolites such as carbohydrate, phenols, tanins and proteins whereas Saponins were absent. It also showed antibacterial activities against almost all the test organisms. The extracts possessed potent hydroxyl radical scavenging activity against the positive control standard Ascorbic acid. Results denote the presence of hydroxyl radical scavenging principles in the extracts.

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Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d211021 ◽

2020 ◽

Vol 21 (10) ◽

Author(s):

SYAFRIZAL ◽

RICO RAMADHAN ◽

IRAWAN WIJAYA KUSUMA ◽

SAAT EGRA ◽

KUNIYOSHI SHIMIZU ◽

...

Keyword(s):

Stingless Bees ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Stingless Bee ◽

Phytochemical Screening ◽

Radical Scavengers ◽

Tropical Rain Forests ◽

Dpph Assay ◽

Research Areas ◽

Light Yellow

Abstract. Syafrizal, Ramadhan R, Kusuma IW, Egra S, Shimizu K, Kanzaki M, Arung ET. 2020. Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia. Biodiversitas 21: 4623-4630. Kalimantan Island in Indonesia is covered by tropical rain forests that are rich in biodiversity. Species, include a stingless bee (Trigona spp.). Recently, stingless bee beekeeping (Meliponiculture) in this region has become popular because the honey has a unique taste, a mix of sweet, sour, and bitter which is highly valued. The objective of this study was to determine the diversity of stingless bee species, the number of colonies, type of hive, and honey properties such as taste, acidity, color, phytochemicals, and radical scavenging activity or antioxidant in meliponiculture in selected areas of Eastern and Northern Kalimantan, Indonesia. The area studied included Samarinda, Balikpapan, Penajam, Bontang, Sangatta, and Tarakan. The study conducted by visiting the farmers, collecting the stingless bees species and bee products (honey, propolis, and bee pollen), pH, color and taste determination, phytochemical screening, and DPPH assay. We found 12 species, with Heterotrigona itama and Tetragonula laeviceps being the main ones cultivated by people in all research areas, except Balikpapan. We counted 111 colonies of T. laeviceps and 99 of H. itama in the meliponiculture. There were three types of hive used by farmers in meliponiculture. Most of the honey tasted sweet and sour with acidity or pH ranging from 3-4, and color from dark brown to light yellow. The phytochemicals contained tannin, alkaloid, flavonoid, triterpenoid, carotenoid, coumarin, saponin, and carbohydrate. The Tetragonula sarawakensis honey was the most potent in term radical scavengers. Our findings showed that stingless bee honey contains antioxidants with phytochemicals that are very useful for human health.

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Fractionation, Phytochemical Screening and Free Radical Scavenging Capacity of Different Sub-Fractions from Pituranthos scoparius Roots

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i3.4100 ◽

2020 ◽

Vol 10 (3) ◽

pp. 133-136

Author(s):

Ahlem Karbab ◽

Kamel Mokhnache ◽

Lekhmici Arrar ◽

Abderahmane Baghiani ◽

Seddik Khennouf ◽

...

Keyword(s):

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Health Benefit ◽

Natural Antioxidants ◽

Chloroform Extract ◽

Free Radical Scavenging ◽

Scavenging Activity ◽

Phytochemical Screening ◽

Potential Health

The purpose of this study was to prepare three sub-fractions from Pituranthos scoparius roots (PSR), characterize their phytochemicals contents and to investigate their free radical scavenging activity by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl scavenging activities. Tannins, ﬂavonoids, steroids, and other bioactive compounds were found in the different sub-fractions. The Ethyle acetate extract (EAE) and chloroform extract (ChE) exhibited the highest antioxidant activity using ABTS (17.8 ± 0.87 µg/mL and 18.15 ± 0.68 µg/mL), respectively. Whereas, Crude extract (CrE) have been presented strong hydroxyl scavenging activity (14.9 ± 0.8 µg/mL). This study indicates that PSR extracts has potent free radical scavenging, and may prove to be of potential health benefit as well as additional resources for natural antioxidants. Keywords: Medicinal plant; phytochemical screening; sub-fractions, free radical scavenging.

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Phytochemical screening and In-vitro antioxidant activity of Centella asiatica extracts

International Journal of Phytomedicine ◽

10.5138/09750185.2123 ◽

2017 ◽

Vol 9 (4) ◽

pp. 615

Author(s):

Mukesh Kumar Yadav ◽

Santosh Kumar Singh ◽

JS Tripathi ◽

YB Tripathi

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Centella Asiatica ◽

Free Radical Scavenging ◽

Scavenging Activity ◽

Phytochemical Screening

Centella asiatica also known as mandukparni or Indian pennywort or jalbrahmi, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. Centella asiatica has long been used to improve memory and cognitive function.The study aimed to identify the phytochemicals present in different solvent extracts of Centella asiatica (i.e. PECA- Petroleum ether extract of C. asiatica, CCA- Chloroform extract of C. asiatica, EACA- Ethyl acetate extract of C. asiatica, ECA- Ethanolic extract of C. asiatica, HACA- Hydro-alcoholic extract of C. asiatica) and evaluate the respective in-vitro antioxidant potentials. The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of Centella asiatica was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.In all different solvent extracts of C. asiatica revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with EC50 values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC50 values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity. The present study indicates that the Centella asiatica extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.

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