Culex vishnui Sebagai Vektor Filariasis Potensial di Kabupaten Kuantan Singingi, Provinsi Riau

Abstract. Kuantan Singingi District is one of the endemic areas of filariasis in Riau Province. Mass treatment activities have been done, but the results of TAS-1 in 2016 with Brugia Rapid Test still found 11 positive children, this condition indicates that there is still new transmission in Kuantan Singingi Regency. The purpose of this study was to determine the mosquito species which potentially become vector filariasis - and to determine the types of potential breeding habitats for mosquito larvae. This research was done in Pulau Panjang Cerenti Village, Cerenti District and Sukadamai Village, Singingi Hilir District in September and November 2017. Catching mosquitoes held for 12 hours starting at 18.00 -06.00 WIB, using the modification of human landing collection double net method. Mosquito catching was done twice, with an interval of one month, at three points/locations for two consecutive nights. There were 24 species of mosquito caught in the two villages. The most dominant species in Pulau Panjang Village was Culex vishnui (54.89%) with indoor and outdoor MHD were 4.5; 16.08 species/man/hour, Sukadamai Village was Culex quinquefasciatus (95.42%) with indoor and outdoor MHD were 23.58; 19.08 species/man/hour. PCR examination results on mosquitoes caught in Sukadamai Village was found microfilarian DNA B. malayi in Cx.vishnui, so potentially become filariasis vector. Breeding habitats that are found mostly was fish ponds that are no longer used, puddles, and swamps. Riverfront habitats was only found in Pulau Panjang Village. Keywords: filariasis, Culex vishnui, potential vector, habitats, Kuantan Singingi Abstrak. Kabupaten Kuantan Singingi merupakan satu diantara wilayah endemis filariasis di Provinsi Riau. Kegiatan pengobatan massal telah dilakukan, namun hasil TAS-1 tahun 2016 dengan Brugia Rapid Test masih ditemukan 11 anak positif. Kondisi ini menunjukan bahwa masih terjadi penularan baru di Kabupaten Kuantan Sengingi. Tujuan penelitian ini untuk mengetahui spesies nyamuk yang berpotensi menjadi vektor filariasis dan mengetahui jenis-jenis habitat perkembangbiakan potensial bagi larva nyamuk. Pengumpulan data telah dilakukan di Desa Pulau Panjang Cerenti Kecamatan Cerenti dan Desa Sukadamai Kecamatan Singingi Hilir pada bulan September dan November 2017. Penangkapan nyamuk dilakukan selama 12 jam dimulai dari pukul 18.00 -06.00 WIB, menggunakan metode modifikasi human landing collection double net. Penangkapan nyamuk dilakukan sebanyak dua kali, dengan selang waktu satu bulan, pada tiga titik/lokasi selama dua malam berturut-turut. Spesies nyamuk yang tertangkap di dua desa sebanyak 24 spesies. Spesies yang paling dominan di Desa Pulau Panjang yaitu Culex vishnui (54,89%) dengan MHD di luar dan dalam rumah yaitu 4,5 dan 16,08 ekor/orang/jam, sedangkan di Desa Sukadamai yaitu Cx. quinquefasciatus (95,42%) dengan MHD di luar dan dalam rumah yaitu 23,58 dan 19,08 ekor/orang/jam. Hasil pemeriksaan PCR pada nyamuk yang tertangkap di Desa Sukadamai ditemukan DNA mikrofilaria B. malayi pada nyamuk Cx. vishnui sehingga berpotensi menjadi vektor filariasis. Habitat perkembangbiakan yang ditemukan sebagian besar adalah kolam ikan yang sudah tidak digunakan lagi, genangan air, dan rawa. Habitat tepi sungai hanya ditemukan di Desa Pulau Panjang. Kata Kunci: filariasis, Culex vishnui, vektor potensial, habitat, Kuantan Singingi

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Biting Pattern and Seasonality of Filariasis Vector of Mansonia spp. in Endemic Area of Banyuasin Regency, South Sumatera, Indonesia

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.6138 ◽

2021 ◽

Vol 9 (E) ◽

pp. 509-517

Author(s):

Rini Pratiwi ◽

Adri Huda ◽

Ahmad Ghiffari ◽

Chairil Anwar

Keyword(s):

Endemic Area ◽

Dominant Species ◽

Control Programme ◽

Vector Control Programme ◽

Human Landing ◽

Mosquito Behaviour ◽

Mansonia Uniformis ◽

Filariasis Vector ◽

Biting Behaviour ◽

Indoor And Outdoor

The diversity and biting activity of Mansonia is important to be determined as to predict the incriminated vector and pattern the mosquito behaviour in establishing the vector control programme. The present study has been successfully conducted to investigate the prolonged biting behaviour of Mansonia spp. in two villages in Banyuasin Regency, South Sumatera Province-Indonesia, using Human Landing Collection method (HLC) in indoor and outdoor for 14 months. The result shows that there are 4956 Mansonia spp. mosquitoes collected during the study comprising 6 species: Mansonia uniformis (40.37%), Mansonia annulifera (32.04%), Mansonia indiana (19.97%), Mansonia bonneae (5.28), Mansonia dives (2.14%); and Mansonia annulata (0.18%). The further investigation confirms that Mansonia uniformis (41.254%) and Mansonia bonneae (45.490%) become the most dominant species caught in Sedang village and Muara Sugih village, respectively and find higher biting activities in outdoor than indoor with biting peaked time at approximately 18.00-19.00. However, the biting activities is also observed in daytime, indicating the behaviour change of Mansonia spp. as the nocturnal mosquitoes. Furthermore, the periods of the highest biting rates are found in April 2017 and May 2017 in Sedang village and May 2018 in Muara Sugih Village. The high period of biting activities and behaviours become the valuable information to arrange the further controlling action of filariasis transmission in Sedang and Muara Sugih Villages as the endemic area of filariasis in South Sumatera, Indonesia.

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KEANEKARAGAMAN NYAMUK VEKTOR FILARIASIS LIMFATIK DI WILAYAH ENDEMIS KABUPATEN PELALAWAN PROVINSI RIAU

Vektora Jurnal Vektor dan Reservoir Penyakit ◽

10.22435/vk.v12i2.3547 ◽

2020 ◽

Vol 12 (2) ◽

pp. 105-118

Author(s):

Maya Arisanti ◽

Anif Budianto ◽

Rahayu Hasti Komaria ◽

Katarina Sri Rahayu ◽

Rizki Nurmaliani

Keyword(s):

Polymerase Chain Reaction ◽

Culex Quinquefasciatus ◽

Mosquito Species ◽

Rapid Test ◽

Brugia Malayi ◽

Chain Reaction ◽

Predominant Species ◽

Human Landing ◽

Net Method ◽

Polymerase Chain

Mass Drug Administration (MDA ) for lymphatic filariasis (LF) control was completed in 2016, however, the result of the Transmission Assessment Survey-1 (TAS -1) with the Brugia Rapid Test confirmed that 17 children were positive. This shows that LF transmission is still going on in Pelalawan District. The study aimed to identify the diversity of mosquito species that responsible for LF transmission in Pelalawan District. Data were collected from Sialang Bungkuk Village and Ukui Village 1 in September and November 2017. Mosquitoes were captured using the modified human landing collection with a double net method for 12 hours from 6 pm to 6 am. Catching mosquitoes carried out twice with an interval of 1 month at two fishing locations. Detection of Deoxyribonucleic Acid (DNA) of Brugia malayi in all types of mosquitoes using Polymerase Chain Reaction (PCR). A total of 1,276 adult mosquitoes was caught in these two study locations. They consisted of 25 species. Mansonia dives was the predominant species in Sialang Bungkuk Village with outdoor Man Hour Density (MHD) 17.67 mosquitoes/person/hour, while Armigeres kesseli was the predominant species in Ukui 1 village with outdoor MHD 25.68 mosquitoes/person/hour. the estimated age of the mosquito in Sialang Bungkuk Village ranged from 4,24 to 32,83 days. Among them, the oldest mosquito species was Culex gellidus, while Culex nigropunctatus was identified as the oldest mosquito in Ukui 1 village 0-7,82 days. DNAs were detected among Ma. dives and Culex. quinquefasciatus. The potential mosquito habitats found in two locations were found at swamps, rubber soaking ponds, ripples in rubber gardens, unused pools. We concluded that these species were responsible for filariasis transmission in that habitats. Abstrak Pemberian Obat Pencegahan Massal (POPM) filariasis di Kabupaten Palalawan telah selesai dilaksanakan tahun 2016, akan tetapi setelah dilakukan survei Transmission Assesment Survey-1 (TAS-1), ditemukan tujuh belas anak positif mikrofilaria. Hasil tersebut menunjukkan masih adanya penularan filariasis di kabupaten tersebut. Penelitian ini bertujuan mengidentifikasi keanekaragaman spesies nyamuk yang berpotensi menjadi vektor filariasis limfatik di Kabupaten Pelalawan. Data dikumpulkan dari Desa Sialang Bungkuk dan Kelurahan Ukui Satu pada bulan September dan November 2017. Penangkapan nyamuk dilakukan menggunakan metode modifikasi human landing collection, menggunakan double net selama dua belas jam, pada pukul 18.00-06.00 WIB. Penangkapan dilakukan dua kali dalam selang waktu satu bulan. Deteksi Brugia malayi pada semua jenis nyamuk tertangkap dilakukan menggunakan metode Polymerase Chain Reaction (PCR). Jumlah nyamuk dewasa tertangkap di dua lokasi sebanyak 1.276 ekor, terdiri dari 25 spesies. Spesies nyamuk yang mendominasi di Desa Sialang Bungkuk adalah Mansonia dives dengan Man Hour Density (MHD) luar rumah 17,67 nyamuk/orang/jam, sedangkan di Kelurahan Ukui Satu, spesies nyamuk dominan adalah Armigeres kesseli dengan MHD luar rumah 25,68 nyamuk/orang/jam. Rentang perkiraan umur nyamuk di Desa Sialang Bungkuk adalah 4,24-32,83 hari, spesies nyamuk yang mempunyai perkiraan umur paling panjang adalah Culex gellidus. Rentang perkiraan umur nyamuk di Kelurahan Ukui Satu adalah 0-7,82 hari, spesies nyamuk yang mempunyai perkiraan umur paling panjang adalah Culex nigropunctatus. Hasil pemeriksaan PCR menunjukkan B.malayi terdeteksi pada Ma. dives dan Culex quinquefasciatus. Habitat potensial nyamuk di dua lokasi adalah rawa-rawa, kolam perendaman karet, kobakan di kebun karet, dan kolam yang tidak terpakai. Kesimpulan dari penelitian ini adalah jenis nyamuk di habitat tersebut berpotensi sebagai vektor filariasis

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Keanekaragaman Jenis dan Perilaku Nyamuk pada Daerah Endemis Filariasis di Kabupaten Barito Kuala, Provinsi Kalimantan Selatan

BALABA JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA ◽

10.22435/blb.v14i1.296 ◽

2018 ◽

pp. 31-42 ◽

Cited By ~ 1

Author(s):

Juhairiyah Juhairiyah ◽

Syarif Hidayat ◽

Budi Hairani ◽

Deni Fakhrizal ◽

Dian Eka Setyaningtyas

Keyword(s):

Risk Factor ◽

Observational Study ◽

Endemic Area ◽

Dominant Species ◽

Mosquito Species ◽

Cross Sectional ◽

Collection Method ◽

Human Landing ◽

Cross Sectional Design ◽

Mansonia Uniformis

Barito Kuala District is one of filariasis endemic area with two mosquito species known as Brugia malayi vector: Culex quinquefasciatus and Mansonia uniformis. Hence, it was necessary to conduct a study in endemic areas of filariasis in Barito Kuala to explore further about diversity of mosquito species and their biting activity. This was an observational study with cross-sectional design performed in two filariasis endemic villages. Mosquitoes were collected by Hand Catches and Human Landing Collection method. There were 12 mosquitoes species of 3 genus found in Antar Raya Village with Cx. tritaeniorhynchus as the most dominant species, while 10 mosquitoes species of 4 genus found in Karya Jadi Village with Ma. uniformis as dominant species. Mansonia uniformis and Cx. quinquefasciatus which found in this study indicated as risk factor for filariasis transmission. Biting activity of Ma. uniformis increased in the afternoon and the morning, while Cx. quinquefasciatus was vary, both endophilic and exophilic.

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Kepadatan dan Keragaman Spesies Nyamuk di Desa Jagaraga Kecamatan Buana Pemaca dan Desa Sukajaya, Kecamatan Buay Rawan, Kabupaten Ogan Komering Ulu Selatan

Jurnal Vektor Penyakit ◽

10.22435/vektorp.v14i1.1286 ◽

2020 ◽

Vol 14 (1) ◽

pp. 37-48

Author(s):

Yahya Yahya ◽

R Irpan Pahlepi ◽

Rahayu Hasti Komariah ◽

Desy Asyati ◽

Surakhmi Oktavia

Keyword(s):

Population Density ◽

Dominant Species ◽

Feeding Habits ◽

Rice Fields ◽

Breeding Habitat ◽

Mosquito Larvae ◽

Breeding Habitats ◽

Human Landing ◽

Collection Methods ◽

Resting Behavior

Abstract The study on mosquito diversity was conducted in Jagaraga Village, Buana Pemaca Subdistrict and Sukajaya Village, Buay Rawan Subdistrict of Ogan Komering Ulu Selatan Regency in 2017. This study aimed to analyze population density of mosquitoes, feeding habits and resting behavior of mosquito, and identify the types of potential /specific breeding habitat of mosquito larvae in OKU Selatan regency. Mosquitoes were collected during the night for 12 hours, started at 06.00 p.m. until 06.00 a.m. by human landing, resting, and cattle landing collection methods. The indoor density of mosquitoes ranged from 0.04-3.96 mosquitoes/person/hour with the highest density of 10,7 on Cx.tritaeniorhyncus. Species of mosquitoes collected in Sukajaya Village were more diverse than in Buana Pemaca Village. There were 12 species and the most dominant species was Cx. tritaeniorhyncus (68.09%), Breeding habitats were identified in rice fields, bricks pit, puddle marsh, and flow of rice fields. Abstrak Penelitian tentang keragaman nyamuk telah dilakukan Di Desa Jagaraga Kecamatan Buana Pemaca dan Desa Sukajaya Kecamatan Buay Rawan Kabupaten OKU Selatan pada tahun 2017. Penelitian ini bertujuan untuk mengetahui angka kepadatan populasi nyamuk yang tertangkap, menganalisis kebiasaan mencari makan danperilaku beristirahat masing-masing spesies nyamuk yang tertangkap serta mengetahui jenis-jenis habitat perkembangbiakan potensial bagi larva nyamuk yang ada di Kabupaten OKU Selatan. Penangkapan nyamuk dilakukan selama 12 jam dimulai dari pukul 18.00 WIB malam hingga pukul 06.00 pagi, menggunakan metode human landing collection, resting collection dan penangkapan di sekitar kandang ternak. Spesies nyamuk yang tertangkap 12 spesies dan yang paling dominan yaitu Culex tritaeniorhyncus(68,9%). Kepadatan nyamuk yang menggigit per orang per jam (MHD) di dalam rumah berkisar antara 0,04-3,96 ekor/orang/jam. Kepadatan rata-rata paling tinggi pada nyamuk Cx. tritaeniorhyncus 10,7 ekor. Habitat perkembangbiakan berupa sawah, lubang galian tanah liat untuk batu bata, kobakan, aliran sawah.

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KEANEKARAGAMAN JENIS NYAMUK Anopheles DI SEMBILAN KABUPATEN (TAHAP PRE-ELIMINASI MALARIA) DI PROVINSI SUMATERA SELATAN

Vektora Jurnal Vektor dan Reservoir Penyakit ◽

10.22435/vk.v12i1.2621 ◽

2020 ◽

Vol 12 (1) ◽

pp. 41-52

Author(s):

Yahya Yahya ◽

Didit Haryanto ◽

Rahman Irpan Pahlevi ◽

Anif Budiyanto

Keyword(s):

Environmental Management ◽

Larval Stage ◽

Mosquito Species ◽

Anopheles Mosquito ◽

Fish Ponds ◽

Habitat Types ◽

Anopheles Mosquitoes ◽

Breeding Habitats ◽

Irrigation Channels ◽

Anopheles Vagus

The province of South Sumatra is still one of the malaria endemic areas in Indonesia. The identification and mapping of malaria receptive areas have been conducted by the Provincial Health Office of South Sumatra to monitor the level of malaria receptivity in all districts/municipalities. This research was conducted in October to November 2018 in 9 districts with low malaria endemicity (Lubuklinggau City, Mura, Muratara, OKU, South OKU, East OKU, Muba, Muara Enim, and Lahat) and have not yet achieved malaria elimination. The purpose of this study was to obtain information about Anopheles mosquito species and the types of Anopheles larvae breeding habitats. Mosquito identification was carried out on adult female Anopheles mosquitoes that are captured in the vicinity of cattle pens and the results of breeding from the larval stage. The results showed that larvae and adult Anopheles were still found. Anopheles habitat types found include irrigation channels, paddy fields, paddy water flow, fish ponds, buffalo pools, swamps, and lakes. The species of Anopheles captured were Anopheles vagus, An. barbisrostris, An. tesselatus, An. subpictus, An. nigerrimus, An. kochi, An. umbrosus, An. barbumbrosus and An. maculatus. Anopheles mosquito surveillance activities must still be done regularly to prevent malaria transmission. Reactivating abandoned fish ponds and environmental management can be an alternative effort in controlling Anopheles. Abstrak Provinsi Sumatera Selatan masih termasuk salah satu wilayah endemis malaria di Indonesia. Dinas Kesehatan Provinsi Sumatera Selatan telah melakukan identifikasi dan pemetaan daerah reseptif malaria untuk memantau tingkat reseptivitas malaria di kabupaten/kota. Penelitian ini telah dilakukan pada bulan Oktober hingga November 2018 di sembilan kabupaten yang belum mencapai eliminasi malaria dan termasuk dalam endemisitas rendah (Kota Lubuklinggau, Mura, Muratara, OKU, OKU Selatan, OKU Timur, Muba, Muara Enim dan Lahat). Tujuan penelitian ini adalah untuk mendapatkan informasi mengenai spesies nyamuk Anopheles serta jenis-jenis habitat perkembangbiakan larva Anopheles. Identifikasi dilakukan pada nyamuk Anopheles betina dewasa hasil penangkapan di sekitar kandang ternak serta hasil pembiakan dari stadium larva. Hasil menunjukkan bahwa masih ditemukan larva dan Anopheles dewasa. Jenis habitat Anopheles yang ditemukan meliputi parit, sawah, aliran air pinggir sawah, kolam ikan, kubangan kerbau, kobakan, rawa, serta danau. Spesies Anopheles yang tertangkap adalah Anopheles vagus, An. barbisrostris, An. tesselatus, An. subpictus, An. nigerrimus, An. kochi, An. umbrosus, An. barbumbrosus dan An. maculatus. Kegiatan pemantauan nyamuk Anopheles secara berkala setiap bulan tetap harus dilakukan untuk mencegah terjadinya penularan malaria. Mengaktifkan kembali kolam ikan yang tidak dimanfaatkan dan manajemen lingkungan dapat menjadi upaya alternatif dalam pengendalian Anopheles.

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Dengue-2 and Guadeloupe Mosquito Virus RNA Detected in Aedes (Stegomyia) spp. Collected in a Vehicle Impound Yard in Santo André, SP, Brazil

Insects ◽

10.3390/insects12030248 ◽

2021 ◽

Vol 12 (3) ◽

pp. 248

Author(s):

Marina E. O. Rangel ◽

Luana P. R. Oliveira ◽

Aline D. Cabral ◽

Katharyna C. Gois ◽

Marcos V. M. Lima ◽

...

Keyword(s):

Urban Areas ◽

Mosquito Species ◽

Infection Rates ◽

Serra Do Mar ◽

Virus Rna ◽

High Infection ◽

Human Landing ◽

Environmental Protection Area ◽

Protection Area ◽

Minimum Infection Rate

In 2018–2019, we conducted mosquito collections in a municipal vehicle impound yard, which is 10 km from the Serra do Mar Environmental Protection Area in Santo André, SP, Brazil. Our aim is to study arboviruses in the impound yard, to understand the transmission of arboviruses in an urban environment in Brazil. We captured the mosquitoes using human-landing catches and processed them for arbovirus detection by conventional and quantitative RT-PCR assays. We captured two mosquito species, Aedes aegypti (73 total specimens; 18 females and 55 males) and Ae. albopictus (34 specimens; 27 females and 7 males). The minimum infection rate for DENV-2 was 11.5 per 1000 (CI95%: 1–33.9). The detection of DENV-2 RNA in an Ae. albopictus female suggests that this virus might occur in high infection rates in the sampled mosquito population and is endemic in the urban areas of Santo André. In addition, Guadeloupe mosquito virus RNA was detected in an Ae. aegypti female. To our knowledge, this was the first detection of the Guadeloupe mosquito virus in Brazil.

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Assessment of Mosquito Collection Methods for Dengue Surveillance

10.21203/rs.3.rs-101828/v1 ◽

2020 ◽

Author(s):

Triwibowo Ambar Garjito ◽

Lulus Susanti ◽

Mujiyono Mujiyono ◽

Mega Tyas Prihatin ◽

Dwi Susilo ◽

...

Keyword(s):

Dengue Virus ◽

Mosquito Species ◽

Statistical Significance ◽

Mixed Data ◽

Adult Mosquito ◽

Published Data ◽

Field Samples ◽

Mosquito Collection ◽

Human Landing ◽

Collection Methods

Abstract BackgroundSeveral methods exist to collect and assess the abundance of dengue vector mosquitoes, i.e. morning adult collection using an aspirator, pupal collection, various ovitraps, whole night collection using human landing methods, and larval collection. This diversity of methods might be a source of variability and lack of statistical significance when trying to correlate mosquito density and risk of dengue outbreak. There is also a lack of published data regarding the effectiveness of these methods MethodsA mosquito survey was conducted in 39 locations corresponding to 15 dengue endemic provinces in Indonesia. The larval surveys were performed by collecting at least a single Aedes larva from each container, and then reared up until hatching. Three adult mosquito sampling methods were also used, including morning resting collection, human landing collection, animal baited trap. All field samples were tested for dengue. Factor Analysis of Mixed Data (FAMD) was conducted to analyze the effectiveness of the collection methods against mosquito species and dengue incidence.ResultsA total of 44,675 mosquitoes were collected. The single larva method was the most efficient method. Out of a total of 89 dengue-positive pools, the most frequently encountered virus was DENV2, which made up half of the positive samples, followed by DENV3 and DENV1, respectively. FAMD showed that no correlation could be found between any methods and the presence of dengue virus in mosquitoes. Moreover, no correlation could be found between either any methods or the incidence.ConclusionsThere was no concistency in the efficacy of a given method and the incidence of dengue in the human population. There was no correlation between any of the parameters considered, i.e. method, incidence of dengue, location and presence of dengue virus in mosquitoes. This indicates that entomological factors are not reliable indicators.

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LAMA HIDUP DAN POTENSI Culex quinquefasciatus SEBAGAI VEKTOR FILARIASIS LIMFATIK BERDASARKAN KETINGGIAN PASCA TRANSMISSION ASSESMENT SURVEY (TAS) DI KABUPATEN SUBANG, JAWA BARAT

Vektora Jurnal Vektor dan Reservoir Penyakit ◽

10.22435/vk.v12i2.3241 ◽

2020 ◽

Vol 12 (2) ◽

pp. 155-166

Author(s):

Endang Puji Astuti ◽

Mutiara Widawati ◽

Yuneu Yuliasih ◽

Andri Ruliansyah ◽

Asep Jajang Kusnandar

Keyword(s):

Lymphatic Filariasis ◽

Mosquito Species ◽

Evaluation Study ◽

P Value ◽

Mosquito Abundance ◽

Biting Rate ◽

Human Landing ◽

The Relationship ◽

Density Behavior ◽

Entomological Data

The study on the bioecology of Lymphatic Filariasis (LF) vector is necessary to be used as a support to eliminate LF due to the lack of entomological data for LF vector in Subang, especially after the Provision of Mass Drug Administration for Filariasis Prevention. This study aims to identify mosquito species, density, behavior, longevity, presence of the filaria worm in mosquitoes, and to identify the distribution of mosquitoes in the highlands and lowlands in selected villages in Subang district. This study is part of Lymphatic filariasis (multicenter) evaluation study in Indonesia. The data was collected by using human landing collection method and vector habitat survey data. The entomological data analyzed from the calculation of Man hour density (MHD), Man biting rate (MBR), and longevity, while the relationship between variables and mosquito abundance using Pearson's correlation. The results indicated that the density of mosquitoes caught in Rancahilir was higher than Curug rendeng villages, these results are similar to the LF vector suspect mosquito (Cx. quinquefasciatus). These mosquitoes are found outdoor with peak densities at 22.00-23.00 West Indonesia Time. Altitude has an association with the density of mosquitoes (p-value 0.039). The longevity of Cx. quinquefasciatus population is 10-13 days. At that longevity, vectors are at risk for infective filarial worms. The result shows that Cx. quinquefasciatus collected in this study was negative for filarial worms. Based on the results of this study, we conclude that in Subang, filariasis transmission chain prevention has been successful. However, periodic evaluations is still need to be carried out in order to prevent re-infection of the disease. Abstrak Kajian tentang bioekologi nyamuk vektor Lymphatic Filariasis (LF) di Kabupatem Subang perlu dilakukan. Kajian ini sebagai pendukung untuk menuju eliminasi LF mengingat belum optimalnya data entomologi nyamuk vektor di Subang, terutama pasca Pemberian Obat Masal Pencegahan (POMP) filariasis. Penelitian ini bertujuan mengidentifikasi spesies, kepadatan, perilaku, umur panjang (longevity) populasi nyamuk, identifikasi keberadaan cacing filaria pada nyamuk vektor LF serta menganalisis perbedaan distribusi nyamuk pada dataran tinggi dan rendah di desa terpilih di Kabupaten Subang. Penelitian ini merupakan analisis lanjut dari penelitian “Studi evaluasi filariasis (multicenter) di Indonesia” yang dilaksanakan di beberapa kabupaten di Indonesia. Data yang diambil, yaitu data survei vektor yang menggunakan metode Human Landing Collection (HLC) dan survei habitat vektor. Analisa data entomologi menggunakan perhitungan Man hour density (MHD), Man-biting rate (MBR) dan nilai umur panjang nyamuk (longevity), sedangkan hubungan antar variabel dengan kepadatan menggunakan korelasi pearson. Hasil penelitian menunjukkan bahwa kepadatan nyamuk yang tertangkap di Desa Rancahilir (dataran rendah) lebih banyak dibandingkan di Desa Curug rendeng (dataran tinggi), termasuk kepadatan nyamuk Cx. quinquefasciatus yang juga dominan di wilayah ini. Nyamuk ini banyak ditemukan di luar rumah dengan puncak kepadatan pada jam 22.00-23.00 WIB. Ketinggian mempunyai hubungan dengan kepadatan nyamuk tertangkap (p value 0,039). Umur populasi nyamuk Cx. quinquefasciatus 10-13 hari. Umur ini menunjukkan umur populasi yang berisiko untuk infektif cacing filaria. Hasil pemeriksaan nyamuk Cx. quinquefasciatus negatif terhadap cacing filarial. Hasil ini menunjukkan bahwa di Kabupaten Subang telah berhasil memutuskan rantai penularan filaria. Namun, masih perlu dilaksanakan evaluasi berkala agar tidak terjadi penularan kembali di wilayah ini.

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Larval breeding habitats and ecological factors influence the species composition of mosquito (Diptera : Culicidae) in the parks of Dhaka city, Bangladesh

Bangladesh Journal of Zoology ◽

10.3329/bjz.v45i2.35706 ◽

2018 ◽

Vol 45 (2) ◽

pp. 111-122 ◽

Cited By ~ 2

Author(s):

Afroza Sultana ◽

Sharmin Hasan ◽

Mosharraf Hossain ◽

Abdul Alim ◽

Mohammad Al Mamun ◽

...

Keyword(s):

Mosquito Control ◽

Mosquito Species ◽

Ecological Factors ◽

Control Program ◽

Breeding Habitat ◽

Coconut Shell ◽

Larval Abundance ◽

Tree Hole ◽

Breeding Habitats ◽

Tree Holes

Mosquito larval ecology is prerequisite for determining the larval abundance and species assemblage in mosquito control program. The study explored the association of five mosquito species with their breeding habitat diversity and species distribution in three selected parks from May to October, 2015. A total of 3217 mosquito larvae were reported from six breeding habitats, namely tree hole, leaf axils, water bottle, tire, drain and coconut shell. The frequency of the three species (Ae. aegypti, Ae. albopictus and Ar. subalbatus) coexistence was higher in tree holes (82.4%) than that of the other coexisting species. Pearson Chi-square result revealed that the association of species was significantly dependent on the breeding habitats. ANOVA further suggested that mosquito density varied across habitats where among the highest density of Cx. quinquefasciatus (3.87 ± 0.22) found in drain, followed by both Ae. albopictus (2.02 ± 0.17) and Ar. subalbatus (0.50 ± 0.09) in tree holes and Ae. aegypti (1.25 ± 0.23) in coconut shell. Cx. tritaeniorhynchus occurred in drain with the least observed density (0.03 ± 0.01). CCA results suggested that Aedes species were likely preferred to oviposit in shaded habitats where pH was associated with Ae. albopictus and dissolved oxygen was with Ae. aegypti and Ar. subalbatus. Culex species were positively associated with the habitats characterizing muddy bottom and emerging vegetation but not with any of the physico-chemical parameters. These findings concluded that ecological factors influence mosquito species to favor their breeding habitats can be helpful in controlling targeted vector species as well as the mosquito borne diseases.Bangladesh J. Zool. 45(2): 111-122, 2017

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Human biting Culex pipiens bioform molestus is spread in several areas in south Sweden

10.1101/616706 ◽

2019 ◽

Author(s):

Luande Verah Nafula ◽

Disa Eklöf ◽

Anders Lindström ◽

Steven Ger Nyanjom ◽

Magnus Evander ◽

...

Keyword(s):

Rural Areas ◽

Urban Areas ◽

Culex Pipiens ◽

Mosquito Species ◽

Urban Environments ◽

Coi Gene ◽

Morphological Identification ◽

Potential Vector ◽

South Sweden ◽

Citizen Science Project

AbstractThe mosquito species Culex pipiens is a potential vector of several pathogens infecting humans and occurs in two distinct bioforms, pipiens and molestus. Traditional morphological identification fails to separate the bioforms of Cx. pipiens despite their behavioural differences since they are morphologically indistinguishable. However, molecular methods can identify the two bioforms. The bioform molestus thrives in urban environments and bite all kinds of vertebrates, whereas bioform pipiens is more rural and mainly feed on birds.Mosquito samples submitted in a citizen science project from people experiencing mosquito problems in South Sweden were analyzed to determine the geographical distribution of the molestus bioform of Cx. pipiens. Mosquito specimens were identified to species by DNA barcoding of the cytochrome C oxidase subunit I (COI) gene and the bioforms were determined with the CQ11 microsatellite marker. To establish other differences between the bioforms, part of the CPIJ001674 gene was sequenced. Culex pipiens f molestus, was present both within and outside of urban areas in several sites in southern Sweden. In one site, hybrids between the two bioforms were found. Culex pipiens f molestus has previously been found in urban areas in Sweden, but the detection of the bioform in several rural areas was surprising, indicating that it may be more widely spread than previously thought.

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