Detection of Zika virus in Anopheles stephensi Liston, 1901 (Diptera: Culicidae) in India - First report

Zika virus (ZIKV) a mosquito-borne, causing acute febrile illness associated with rash, arthralgia and conjunctivitis in the patient, was reported from Thiruvananthapuram, Kerala, as an outbreak with 83 cases. Entomological surveillance revealed the presence of aedine mosquitoes viz., Aedes aegypti (Linnaeus, 1762), Ae. albopictus (Skuse, 1894) and Ae. vittatus (Bigot, 1861) and nonaedine mosquitoes viz., Anopheles stephensi Liston,1901, Mansonia uniformis (Theobald, 1901), Culex tritaeniorhynchus Giles, 1901 and Cx. gelidus Theobald,1901. Aedes (Ae. aegypti, Ae. vittatus and Ae. Albopictus) mosquito larvae were high in the Zika affected areas. Moreover ZIKV was detected in An. stephensi mosquitoes collected from Parassala, Thiruvananthapuram (the native place of the first ZIKV confirmed case in the present outbreak in Kerala). Molecular diagnostics of Ae. Aegypti, Ae. vittatu and An. stephensi mosquitoes revealed that the species were loaded with ZIKV. Significantly this is the first ever report of ZIKV detecting in An. stephensi in the world. Aedes adults (male and female) and An. stephensi emerged from fourth instar larvae and pupae were found to have ZIKV, indicating transovarial transmission of the virus.

Fauna associated with Malayan filariasis transmission in Banyuasin, South Sumatra, Indonesia

Background and Aim: Brugia malayi is known to be zoonotically important because it can be transmitted from animals (mammals and primates) to humans or from humans to humans through mosquito vectors. This study was conducted to explore the fauna associated with Malayan filariasis transmission in Sedang village, Suak Tapeh District, Banyuasin Regency, South Sumatra Province, Indonesia. Materials and Methods: A cross-sectional research design with an observational and analytical approach was applied in this study, and it was conducted in May 2018. Mosquitoes were collected twice using human bait both inside and outside the house from 6:00 p.m. to 6:00 a.m. The presence of competitors, predators, and reservoir hosts in the areas of five breeding habitats of Mansonia spp. was observed. The presence of microfilaria was confirmed under a microscope in night blood samples of inhabitants and cats. The presence of infective larvae (L3) of B. malayi was identified microscopically and based on the polymerase chain reaction method in female Mansonia mosquitoes. Results: A total of 12 mosquito species were found, among which Mansonia uniformis was the dominant mosquito, and the predominant competitor was Mansonia annulifera. Dragonflies, as predators were found in two breeding habitats and fish were found in one breeding habitat. The L3 of B. malayi were not identified in the mosquitoes, and the microfilariae of B. malayi were not found in the blood samples of inhabitants and cats. Conclusion: Although Mansonia mosquito population was abundant in Banyuasin Regency, the mosquito was not confirmed as an intermediate host of B. malayi, and the cat was not confirmed as a reservoir of B. malayi in the location.

Biting Pattern and Seasonality of Filariasis Vector of Mansonia spp. in Endemic Area of Banyuasin Regency, South Sumatera, Indonesia

The diversity and biting activity of Mansonia is important to be determined as to predict the incriminated vector and pattern the mosquito behaviour in establishing the vector control programme. The present study has been successfully conducted to investigate the prolonged biting behaviour of Mansonia spp. in two villages in Banyuasin Regency, South Sumatera Province-Indonesia, using Human Landing Collection method (HLC) in indoor and outdoor for 14 months. The result shows that there are 4956 Mansonia spp. mosquitoes collected during the study comprising 6 species: Mansonia uniformis (40.37%), Mansonia annulifera (32.04%), Mansonia indiana (19.97%), Mansonia bonneae (5.28), Mansonia dives (2.14%); and Mansonia annulata (0.18%). The further investigation confirms that Mansonia uniformis (41.254%) and Mansonia bonneae (45.490%) become the most dominant species caught in Sedang village and Muara Sugih village, respectively and find higher biting activities in outdoor than indoor with biting peaked time at approximately 18.00-19.00. However, the biting activities is also observed in daytime, indicating the behaviour change of Mansonia spp. as the nocturnal mosquitoes. Furthermore, the periods of the highest biting rates are found in April 2017 and May 2017 in Sedang village and May 2018 in Muara Sugih Village. The high period of biting activities and behaviours become the valuable information to arrange the further controlling action of filariasis transmission in Sedang and Muara Sugih Villages as the endemic area of filariasis in South Sumatera, Indonesia.

Biodiversity of mosquitoes and Mansonia uniformis as a potential vector of Wuchereria bancrofti in Hulu Sungai Utara District, South Kalimantan, Indonesia

Background and Aim: Lymphatic filariasis, also known as elephantiasis, still remains a problem in Indonesia. The primary causative species of this disease are the filarial worms Wuchereria bancrofti and Brugia spp. This study was conducted to identify the diversity of species and behavior of mosquitoes and to determine the mosquitoes that could be potential vectors of filariasis. Materials and Methods: Mosquito samples derived from Hulu Sungai Utara (HSU) district in the 2017 multicenter study conducted in Indonesia were used in this cross-sectional study. The diversity of mosquito species was analyzed using the Shannon–Wiener diversity index. Mosquitoes were identified based on their species, and their DNA was isolated by polymerase chain reaction (PCR). Transcription-insulated isothermal PCR method was used to detect microfilariae/filaria larvae in the mosquitoes. Results: Biodiversity was found in 14 species of mosquitoes belonging to five genera. The maximum number of mosquitoes was recorded from the species Mansonia dives, Culex vishnui, Culex quinquefasciatus, and Mansonia uniformis. W. bancrofti infection was detected in M. uniformis at an infectivity rate of 0.3% (n=311). Conclusion: To the best of our knowledge, this is the first report of M. uniformis species as a vector of W. bancrofti in HSU district, Indonesia. More efficient and accurate studies are required to aid in the lymphatic filariasis elimination programs in this subregion.

Keanekaragaman dan perilaku menggigit nyamuk sebagai vektor potensial filariasis di Kabupaten Banyuasin, Sumatera Selatan

Filariasis adalah penyakit menular menahun disebabkan oleh cacing filaria dan ditularkan oleh berbagai spesies nyamuk seperti Mansonia, Anopheles, Culex, Aedes, dan Armigeres. Penelitian ini bertujuan mengetahui keanekaragaman dan perilaku menggigit nyamuk sebagai vektor potensial penularan filariasis di Kabupaten Banyuasin, Sumatera Selatan. Penangkapan nyamuk dilakukan selama 12 jam dari pukul 18.00–06.00 dengan metode human landing collection dan animal biting trap. Pada Desember 2016 hasil penelitian menunjukkan bahwa sampel nyamuk yang ditangkap di daerah studi berjumlah 16 spesies dengan spesies paling dominan yang ditemukan adalah Mansonia uniformis (47,28%), Culex tritaeniorhynchus (23,89%), dan Culex vishnui (14,48%). Ma. uniformis memiliki perilaku menggigit lebih banyak di luar rumah dengan rata-rata angka perilaku menggigit (man biting rate/MBR) sebesar 100,50 nyamuk/malam dan angka kepadatan gigitan nyamuk (man hour density/MHD) sebesar 11,90 dan merupakan yang tertinggi dari semua spesies nyamuk yang ditemukan. Analisis parity rate melalui pembedahan terhadap nyamuk dewasa mengkonfirmasi bahwa perkiraan umur populasi Ma. uniformis adalah 31,8 hari dan Ma. uniformis terdeteksi positif sebagai vektor penularan filariasis.

IDENTIFIKASI JENIS NYAMUK DAN KARAKTERISTIK HABITATNYA DI DESA KEKERI KECAMATAN GUNUNG SARI KABUPATEN LOMBOK BARAT

Nyamuk merupakan salah satu serangga yang berperan sebagai vektor penyakit melalui gigitannya. Penyakit malaria, Demam Berdarah Dengue (DBD), dan filariasis merupakan beberapa penyakit yang ditularkan nyamuk. Kecamatan Gunung Sari memiliki angka kasus DBD sebanyak 27 kasus dan malaria sebanyak 1.078 suspect pemeriksaan dengan hasil 29 kasus positif malaria. Desa Kekeri merupakan salah satu desa yang termasuk dalam kawasan Kecamatan Gunung Sari. Tujuan penelitian ini adalah untuk mengetahui jenis nyamuk yang teridentifikasi, karakteristik habitat, dan kelimpahan nyamuk yang ada di Desa Kekeri. Jenis penelitian yang digunakan yaitu deskriptif ekploratif. Teknik pengambilan sampel menggunakan aspirator, titik sampling meliputi 3 jenis habitat yaitu perumahan, persawahan dan ladang. Berdasarkan penelitian yang telah dilakukan, teridentifikasi 6 spesies nyamuk di Desa Kekeri yaitu Culex quinquefasciatus, C. tritaeniorhynchus, C. bitaeniorhynchus, C. sitiens, Aedes aegypty, dan Mansonia uniformis. Karakteristik habitat nyamuk didapatkan di kawasan perumahan, persawahan, dan ladang dengan kisaran suhu rata-rata 26,6°C -27°C, kelembaban 69,3-69,6%, kecepatan angin 14 km/h-14,7 km/h, dan vegetasi ternaungi pepohonan, padi, rerumputan, jagung, dan tanaman sayur (labu, kacang panjang, cabai). Nilai Indeks Kelimpahan Relatif (IKR) tertinggi dimiliki oleh nyamuk Culex tritaeniorhynchus yang dapat ditemukan pada ketiga jenis habitat yaitu sebesar 55,8%. Culex bitaeniorhynchus dan Mansonia uniformis memiliki nilai IKR terendah yaitu sebesar 1,03%.

Bionomik Mansonia uniformis dan Mansonia dives sebagai Vektor Filariasis pada Beberapa Wilayah di Kalimantan

Mansonia mosquitoes spread across the globe and several species as a vector. Ma. uniformis were known a vectors of Rift Valley Fever (RVF) and filariasis, while Ma. dives is vector of filariasis. This research aims to behavior of Ma. uniformis and Ma. dives in the form habits of blood sucking activity, resting behaviour and fluctuations density in several areas of Borneo. Natural population of Ma. uniformis and Ma. dives were collected by human landing collection and resting collection from 18:00 to 06:00 outdoor and indoor. The results showed bite activities. Ma. uniformis and Ma. dives in all areas of research are exophagic, while the rest conduct Ma. uniformis in the village Dadahup, Pulau Ku’u and Bangkal Ulu is indoor resting, while in Mandomai is outdoor resting. Behavior breaks Ma. dives in the area a lot more research that is indoor resting namely Mandomai and Bangkal Ulu Village, while the Dadahup village is exophilic. Highest fluctuations activity suck blood and rest on Ma. Uniformis in and out the house generally at 19.00 - 22.00, while at Ma. dives at 23.00 - 01.00. The behavior of mosquitoes is a difference, so the controlling method is also different.

Keanekaragaman Jenis dan Perilaku Nyamuk pada Daerah Endemis Filariasis di Kabupaten Barito Kuala, Provinsi Kalimantan Selatan

Barito Kuala District is one of filariasis endemic area with two mosquito species known as Brugia malayi vector: Culex quinquefasciatus and Mansonia uniformis. Hence, it was necessary to conduct a study in endemic areas of filariasis in Barito Kuala to explore further about diversity of mosquito species and their biting activity. This was an observational study with cross-sectional design performed in two filariasis endemic villages. Mosquitoes were collected by Hand Catches and Human Landing Collection method. There were 12 mosquitoes species of 3 genus found in Antar Raya Village with Cx. tritaeniorhynchus as the most dominant species, while 10 mosquitoes species of 4 genus found in Karya Jadi Village with Ma. uniformis as dominant species. Mansonia uniformis and Cx. quinquefasciatus which found in this study indicated as risk factor for filariasis transmission. Biting activity of Ma. uniformis increased in the afternoon and the morning, while Cx. quinquefasciatus was vary, both endophilic and exophilic.

Faktor Lingkungan dan Perilaku Masyarakat yang Berhubungan dengan Kejadian Filariasis di Kecamatan Muara Pawan Kabupaten Ketapang Provinsi Kalimantan Barat

Judul : Faktor Lingkungan dan Perilaku Yang Berhubungan Dengan Kejadian Filariasis di Kecamatan Muara Pawan Kabupaten Ketapang, Kalimantan BaratLatar belakang: Tahun 2015, terdapat 79 kasus filariais di Kecamatan Muara Pawan Kabupaten Ketapang dengan micrifilaria rate sebesar 2,5%. Penelitian ini bertujuan untuk menganalisis hubungan antara faktor lingkungan dan perilaku dengan kejadian filariasis, serta mengidentifikasi spesies nnyamuk yang diduga sebagai vektor filariasis.Metode: Penelitian ini merupakan penelitian observasional dengan rancangan case control. Subyek penelitian terdiri dari 32 kasus dan 32 kontrol. Pengumpulan data dilakukan dengan wawancara dan observasi. Analisis data menggunakan uji Chi-square dengan taraf signifikasi 0,05.Hasil: Dari 419 nyamuk yang ditangkap. Tidak ditemukan adanya larva cacing dalam tubuh nyamuk. Hasil identifikasi nyamuk menemukan 6 spesies yaitu Anopheles letifer, Mansonia uniformis, Culex tritaeniorhyncus, Culex vishnui, Culex cressipes, and Mansonia annulifera. Analisis statistik menunjukkan bahwa keberadaan breeding places, resting places, pengetahuan, dan kebiasaan keluar pada malam hari merupakan faktor risiko kejadian filariasis di Kecamatan Muara Pawan Kalimantan Barat.Simpulan: Faktor lingkungan dan perilaku masyarakat merupakan faktor risiko kejadian filariasis di Kecamatan Muara Pawan Kabupaten Ketapang. ABSTRACTTitle: Enviromental and Community Behaviour Factors Associated with the incidence of Lymphatic Filariasis in Muara Pawan Distrct of Ketapang Regency, West KalimantanBackground: In the year of 2015, there were 79 cases of filariasis in Ketapang Regency and in Muara Pawan District, in particular, from 32 cases who contracted microfilariae resulted in Mf rate = 2,5% while for prevalence rate (PR) = 2,18. The objectives of this research were to analyze correlation between evironmental and community behavioral factors associated with cases of filariasis as well as to identify the mosquitos which are being the potential vector of filariasis. Methode: The research was an observational one with a case-control study. The cases and the control of this research were both using 32 respondents. The data were collcted by doing observation and interviews. The collected data were than analyzed using chi-square test. Results: From the surgery of 419 mosquitos, it was revealed that the mosquitoes contained zero filarial larvae, while for the species identification, there were six species of mosquitoes indentified, i.e Anopheles letifer, Mansonia uniformis, Culex tritaeniorhyncus, Culex vishnui, Culex cressipes, and Mansonia annulifera. The resulth of statistical analysis revealed that there were 4 variables associated with the cases of filariasis in Muara Pawan Distict of Ketapang Regency, ie. breeding places (p-value = 0,047), resting places (p-value = 0,007), knowledge (p-value = 0,045), and habit of going out at night (p-value = 0,043)Conclusion: It is concluded that there is a correlation between 4 mentioned variables with the cases of filariasis in Muara Pawan Dictrict of Ketapang Regency.