scholarly journals Prediksi Protein Target Bioaktif Ekstrak Metanol Buah Belimbing (Averrhoa carambola) dalam Regulasi Tekanan Darah

2019 ◽  
Vol 8 (1) ◽  
pp. 9-22
Author(s):  
Siti Munawaroh ◽  
Dian Laila Purwaningroom ◽  
Dianita Rifqia Putri ◽  
Cholik Harun Rosjidi
Keyword(s):  
Mass Spectrometry ◽  
Blood Pressure ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Methanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Pressure Regulation ◽  
Averrhoa Carambola

AbstractBlood pressure regulation is basically control of amount of blood flow to certain tissues according to their metabolic needs. The complexity of the mechanisms involved leading to the assumption that there are many functional and structural proteins involved in blood pressure regulation. Indonesian people, especially those living on the island of Java, usually consume star fruit Averrhoa carambola to reduce blood pressure, but the content of bioactive compounds and the mechanism of their interference with proteins is still unknown. This study aimed to determine the bioactive compound content of Averrhoa carambola's methanol extract and its mechanism of interference with the target proteins. An amount of 200 g Averrhoa carambola dried simplicia was extracted by the maceration method using absolute methanol. The extract was then subjected to phytochemical tests using the Gas Chromatography-Mass Spectrometry (GC-MS) method. Based on the results obtained from the GC-MS phytochemical test, the names of the bioactive compounds found in the Averrhoa carambola methanol extract were obtained. The compounds are then analyzed for their target protein in the human body using the STITCH database (http://stitch.embl.de/). The proteins that had been predicted to be the target of active compounds of Averrhoa carambola methanol extract were analyzed for interactions between proteins using the STRINGdb database (https://string-db.org/cgi/input.pl). Based on this research, it can be concluded that Averrhoa carambola fruit methanol extract can help lower blood pressure by producing NO and acting as antioxidants. However, further research (in-vitro and in-vivo) needs to be done to prove the mechanism. AbstrakRegulasi tekanan darah adalah jumlah kontrol aliran darah ke jaringan tertentu sesuai dengan kebutuhan metabolismenya. Kompleksnya mekanisme yang terlibat, memunculkan asumsi terdapat banyak protein fungsional maupun struktural yang terlibat dalam regulasi tekanan darah. Masyarakat Indonesia, terutama yang tinggal di pulau Jawa biasa mengkonsumsi buah belimbing untuk menurunkan tekanan darah, namun kandungan senyawa bioaktif dan mekanisme intervensinya terhadap protein-protein belum diketahui. Tujuan penelitian ini adalah untuk mengetahui kandungan senyawa bioaktif ekstrak metanol buah Averrhoa carambola dan mekanisme intervensinya terhadap protein-protein targetnya. Simplisia kering buah Averrhoa carambola sebanyak 200 g diekstraksi dengan metode maserasi menggunakan metanol absolut. Dari ekstrak tersebut dilakukan uji fitokimia dengan metode Gas chromatography–mass spectrometry (GC-MS) sehingga didapatkan nama-nama senyawa bioaktif yang terdapat pada ekstrak metanol Averrhoa carambola. Senyawa-senyawa tersebut kemudian dianalisis protein targetnya dalam tubuh manusia menggunakan database STITCH (http://stitch.embl.de/). Kemudian dianalisis interaksi antar protein menggunakan database STRINGdb (https://string-db.org/cgi/input.pl). Berdasarkan penelitian ini, ekstrak metanol buah Averrhoa carambola dapat membantu menurunkan tekanan darah dengan memproduksi NO dan berperan sebagai antioksidan. Namun, penelitian lebih lanjut (in-vitro dan in-vivo) perlu dilakukan untuk membuktikan mekanismenya

scholarly journals ANALISIS GC-MS (GAS CHROMATOGRAPHY - MASS SPECTROMETRY) EKSTRAK METANOL DARI UMBI RUMPUT TEKI (Cyperus rotundus L.)

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 849
Author(s):  
Ellen Hotmian ◽  
Elly Suoth ◽  
Fatimawali Fatimawali ◽  
Trina Tallei
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Cyperus Rotundus ◽  
Gas Chromatography Mass Spectrometry ◽  
The Public ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

ABSTRACTThe Nut Grass Tuber (Cyperus rotundus L.) is a plant that is believed by the public to cure several diseases. According to a study conducted in vivo and in vitro, the extract the tuber root has many potentials such as anticancer, anti-inflammatory, antibacterial, etc. This study aims to determine the polar bioactive compounds contained in the bulb tubers. The method used was the extraction of nut tuber dry powder using methanol as a solvent by maceration process and then analyzed using gas chromatography - mass spectrometry (GC-MS) to obtain information on the content of the tubers The results of GC-MS analysis from this study indicate that there are 177 possible components of the compound extracted using methanol. The results also showed that there were three possible polar bioactive compounds at the highest peak of the GC-MS analysis, namely 7-Isopropenyl-1,4a-dimethyl-4,4a, 5,6, 7,8-hexahydro-3H-naphthalen-2-one, 1 (2H) -Naphthalenone, 3,4,4a, 5,6,7-hexahydro-4a, 5-dimethyl-3- (1-methylethenyl) -, [3S- (3a, 4aa, 5a)] -, and 2 (1H) Naphthalenone, 3,5,6,7,8,8a-hexahydro-4,8a-dimethyl-6- (1-methylethenyl) -.Keywords         : Methanolic extract, nut grass tuber, Cyperus rotundus L., GC-MS   ABSTRAKUmbi Rumput Teki (Cyperus rotundus L.) merupakan tumbuhan yang dipercayai masyarakat dapat menyembuhkan beberapa penyakit. Menurut sebuah penelitian yang dilakukan secara in vivo maupun in vitro, kandungan ekstrak umbi rumput teki memiliki banyak potensi seperti antikanker, antiinflamasi, antibakteri, dll. Penelitian ini bertujuan untuk mengetahui senyawa-senyawa bioaktif polar yang terkandung pada umbi rumput teki. Metode yang digunakan yaitu ekstraksi serbuk kering umbi rumput teki menggunakan pelarut metanol dengan proses maserasi kemudian dianalisis menggunakan kromatografi gas – spektrometri massa (GC-MS) untuk mendapatkan informasi kandungan dalam umbi rumput teki. Hasil analisis GC-MS dari penelitian ini menunjukkan bahwa terdapat 177 kemungkinan komponen senyawa yang diekstraksi menggunakan pelarut methanol. Hasil penelitian juga menunjukkan bahwa terdapat tiga kemungkinan senyawa bioaktif polar pada puncak tertinggi hasil analisis GC-MS, yaitu 7-Isopropenyl-1,4a-dimethyl-4,4a,5,6,7,8-hexahydro-3H-naphthalen-2-one, 1(2H)-Naphthalenone, 3,4,4a,5,6,7-hexahydro-4a,5-dimethyl-3-(1-methylethenyl)-, [3S-(3a,4aa,5a)]-, dan 2(1H)Naphthalenone, 3,5,6,7,8,8a-hexahydro-4,8a-dimethyl-6-(1-methylethenyl)-. Kata kunci      : Ekstrak metanol, umbi rumput teki, Cyperus rotundus L., GC-MS

In vitro and in vivo studies of androst-4-ene-3,6,17-trione in horses by gas chromatography-mass spectrometry

2009 ◽  
Vol 24 (7) ◽  
pp. 744-751 ◽  
Author(s):  
Gary N. W. Leung ◽  
Francis P. W. Tang ◽  
Terence S. M. Wan ◽  
Colton H. F. Wong ◽  
Kenneth K. H. Lam ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
In Vivo Studies ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

scholarly journals Gas Chromatography–Mass Spectrometry Profiling of Volatile Compounds Reveals Metabolic Changes in a Non-Aflatoxigenic Aspergillus flavus Induced by 5-Azacytidine

Toxins ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 57 ◽  
Author(s):  
Fengqin Song ◽  
Qingru Geng ◽  
Xuewei Wang ◽  
Xiaoqing Gao ◽  
Xiaona He ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Volatile Compounds ◽  
Aspergillus Flavus ◽  
Cyclopiazonic Acid ◽  
Metabolic Changes ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Aspergillus flavus is one of the most opportunistic pathogens invading many important oilseed crops and foodstuffs with such toxic secondary metabolites as aflatoxin (AF) and Cyclopiazonic acid. We previously used the DNA methylation inhibitor 5-azacytidine to treat with an AF-producing A. flavus A133 strain, and isolated a mutant (NT) of A. flavus, which displayed impaired abilities of AF biosynthesis and fungal development. In this study, gas chromatography–mass spectrometry (GC-MS) analysis was used to reveal the metabolic changes between these two strains. A total of 1181 volatiles were identified in these two strains, among which 490 volatiles were found in these two strains in vitro and 332 volatiles were found in vivo. The NT mutant was found to produce decreasing volatile compounds, among which most of the fatty acid-derived volatiles were significantly downregulated in the NT mutant compared to the A133 strain, which are important precursors for AF biosynthesis. Two antioxidants and most of the amino acids derived volatiles were found significantly upregulated in the NT mutant. Overall, our results reveal the difference of metabolic profiles in two different A. flavus isolates, which may provide valuable information for controlling infections of this fungal pathogen.

scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA KIMIA SERTA UJI AKTIVITAS ANTICANDIDAISIS SERBUK DAUN SIRIH DUDUK (Piper sarmentosum Roxb. Ex Hunter)

Molekul ◽  
2006 ◽  
Vol 1 (1) ◽  
pp. 19
Author(s):  
Suwandri Suwandri ◽  
Hartiwi Diastuti
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Methanol Extract ◽  
Bioactive Compound ◽  
Gas Chromatography Mass Spectrometry ◽  
Chloroform Fraction ◽  
The People ◽  
Infra Red

Sirih duduk or Piper sarmentosum Roxb. ex Hunter have been long used the people in Indonesia for traditional medicine to cure various diseases. One of them was used to treat fungoid diseases. This research was aimed to examine the anticandidaisis activity from extract of P. sarmentosum leaves and to isolate and identify the anticandidaisis compounds from P. sarmentosum leaves. The isolation bioactive compounds of P. sarmentosumleaves was performed by extraction the powder of P. sarmentosum leaves using methanol and the bioactivity tests were performed against Candida albicans. The methanol extracts was then fractionated using organic solvents such as n-hexane, benzene, chloroform, and ethyl acetate. Identification of the bioactive compounds was carried out using ultraviolet-visible spectrometry, fourier transform infra red spectrometry and gas chromatography-mass spectrometry. The result showed that the chloroform fraction of methanol extract of P. sarmentosum leaves have activity against C. albicans. The fractionation was performed by coloumn chromatography using n-hexane-ethyl acetate(1:1) eluent. A bioactive compound of sinamic acid derivative was 4-ethoxy-2-hidroxy-3,5-dimethoxy sinamic acid could be purely isolated.

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