scholarly journals Uji Aktivitas Antioksidan Ekstrak Etanol Biji Buah Merah (Pandanus Conoideus De Vriese) Asal Kabupaten Poso Sulawesi Tengah

2018 ◽  
Vol 6 (3) ◽  
pp. 170
Author(s):  
Siti Sulaeha ◽  
Minarni R. Jura ◽  
Nurdin Rahman
Keyword(s):  
Vitamin C ◽  
Scientific Information ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Ic50 Values

Red fruit seeds have not been studied and known benefits, therefore it needed scientific information about the content and benefits of red fruit seeds. This study aims to analyze the antioxidant power red fruit seed extract as well determining the IC50 value of red fruit seed extract which has the ability of natural antioxidants. Determination of free radical DPPH (1,1-diphenyl-2-picrylhidrazyl) after the addition of red fruit seeds extract is determined using UV-Vis spectrophotometer. Variations in the concentration of the red fruit seed extract used is 20 ppm, 40 ppm, 60 ppm, 80 ppm and 100 ppm.The positive control used is vitamin C with the same concentration. While the negative control using DPPH solution dissolved in absolute ethanol. The results showed IC50 values obtained for the red fruit seed extract and vitamin C are 223.8 ppm and 1.73 ppm. Based on the IC50 data can be known that vitamin C antioxidants more powerful than red fruit seed extract which belongs to natural antioxidants are very weak.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Semangka (Citrullus lanatus)

2018 ◽  
Vol 7 (2) ◽  
pp. 96
Author(s):  
Sri Mariani ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Citrullus Lanatus ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Control Measurement ◽  
Ic50 Value ◽  
Ic50 Values

The aim of this study is determine the IC50 extracts values of white peel and flesh of red watermelon, white peel and flesh of yellow watermelon as antioxidants. To determine antioxidant activities DPPH reagent was used as well as UV-Vis Spectrophotometry was used to measure vitamin C as a positive control measurement. The samples mixed with ethanol in various concentration of 5, 15, 25 and 35 mg/L. The results showed that the IC50 values for each extract of red watermelon flesh, yellow watermelon flesh, white peel of red watermelon, and white peel of yellow watermelon were 16.619 mg/L, 16.575 mg/L, 14.729 mg/L, and 16.782 mg/L, respectively. Vitamin C as the positive control has IC50 value of 9.526 mg/L. These IC50 values showed that vitamin C had higher antioxidant activity than watermelon extracts, but the extracts of watermelon still categorized in strong natural antioxidants. This study concluded that watermelon is good to be consumed because it is a very strong antioxidant.

scholarly journals Antioxidant Activity Tests of Extract of Principle Crafts (Phaleria macrocarpa)

2020 ◽  
Vol 8 (3) ◽  
pp. 125-129
Author(s):  
Tri Putri ◽  
Anang W. M. Diah ◽  
Afadil Afadil
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Positive Control ◽  
Degenerative Diseases ◽  
Fruit Extract ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Phaleria Macrocarpa ◽  
Central Sulawesi

Phaleria macrocarpa contains secondary metabolite compounds such as alkaloids, terpenoids, saponins, polyphenols, tannins, sterols, and coumarins. Those metabolites are antioxidants and can be efficacious in the healing of various degenerative diseases such as cervical cancer and diabetes. This plant is widely grown in Tindaki Village, Parigi Mautong, Central Sulawesi. The aim of this study was to determine the antioxidant power of those phaleria macrocarpa fruit extract. This study was conducted by using maceration extraction technique with ethanol as solvent, and compound 2,2-difenil-1-picrilhidrazil (DPPH) as source of free radical, and vitamin C as the positive control. The UV-Vis spectrophotometer was used to measure the absorbance of the extract. Various concentrations of the extract used were 5 ppm, 15 ppm, 25 ppm, and 35 ppm. The results showed that the IC50 value of the fruit extract was 28.242 ppm, while the IC50 value of vitamin C was 19.302 ppm. These IC50 values show that vitamin C was a stronger antioxidant activity than the fruit extract, but the fruit extract was still as a very strong natural antioxidant category.

scholarly journals Determination of Total Flavonoid Contents and Antioxidant Activity of Leucaena Leucocephala Leaves's Extract

2020 ◽  
Vol 9 (2) ◽  
pp. 70-77
Author(s):  
Taupik Hidayat ◽  
Baharuddin Hamzah ◽  
Minarni R. Jura
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Leucaena Leucocephala ◽  
Positive Control ◽  
Total Flavonoid ◽  
Antioxidant Vitamin ◽  
Antioxidant Power ◽  
Sample Extract ◽  
Ic50 Values

The study of the determination of total flavonoid contents and antioxidant activity of extract of Leucaena leucocephala leaves had been carried out using UV-Vis spectrophotometry method. This study aimed to determine the total flavonoid contents and the antioxidant power of Leucaena leucocephala extract. Extraction of chemical content from Leucaena leucocephala was carried out by the maceration method using absolute ethanol. The total flavonoid contents from the sample extract were taken up for absorption at wavelength 510 nm and 700 nm. The variation of the concentration of Leucaena leucocephala leaves' extract was respectively 20, 40, 60, and 80 ppm. The results of the study showed that the total flavonoid contents of extracts of Leucaena leucocephala leaves was 2.242 g/100 grams. The antioxidant activity of Leucaena leucocephala leaves extract was carried out using DPPH (1,1-diphenyl-2-picrylhydrazine) and the measurement of absorption was at a wavelength of 517 nm, and by varying the sample concentration. Positive control used was vitamin C solution with the same variation of extract's concentration. The results showed that the IC50 values of Leucaena leucocephala leaves extract and vitamin C were 175.388 and 28.907 ppm. Based on the IC50 data it can be stated that the antioxidant vitamin C was stronger than the Leucaena leucocephala leaves extract which was classified as a weak type of natural antioxidant.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Semangka (Citrullus lanatus)

2018 ◽  
Vol 7 (3) ◽  
pp. 107
Author(s):  
Sri Mariani ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Citrullus Lanatus ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Control Measurement ◽  
Ic50 Value ◽  
Ic50 Values

The aim of this study is to determine the IC50 extracts values of white peel and flesh of red watermelon, white peel and flesh of yellow watermelon as antioxidants. To determine antioxidant activities DPPH reagent was used and UV-Vis spectrophotometry was used to measure Vitamin C as a positive control measurement. The samples mixed with ethanol in a various concentration of 5, 15, 25 and 35 mg/L. The results showed that the IC50 values for each extract of red watermelon flesh, yellow watermelon flesh, white peel of red watermelon, and white peel of yellow watermelon were 16.619, 16.575, 14.729, and 16.782 mg/L, respectively. Vitamin C as positive control has an IC50 value of 9.526 mg/L. These IC50 values showed that vitamin C had higher antioxidant activity than watermelon extracts, but the extracts of watermelon still categorized in strong natural antioxidants. This study concluded that watermelon is good to be consumed because it is a very strong antioxidant.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM)

2017 ◽  
Vol 6 (2) ◽  
pp. 125
Author(s):  
Rizkayanti Rizkayanti ◽  
Anang Wahid M. Diah ◽  
Minarni Rama Jura
Keyword(s):  
Vitamin C ◽  
Moringa Oleifera ◽  
Water Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ic50 Value ◽  
Antioxidant Potency ◽  
Ethanol Extracts ◽  
Moringa Oleifera Lam

Moringa (moringa oleifera Lam) leaves contains many molecules as inhibitors for free radicals such as phenolic compounds (phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, tannins), nitrogen compounds (alkaloids, amines, betalain), vitamins, terpenoids (including carotenoids), and several other endogenous metabolites as antioxidants. This study aimed to determine the antioxidant potency of water and ethanol extracts of moringa (moringa oleifera Lam) leave obtained by maceration and dekok. The concentration of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was analyzed using UV-Vis spectrophotometer after addition of various concentrations of Moringa leaves extracts. Various concentrations of moringa leave extracts used were 20 ppm, 40 ppm, 60 ppm and 80 ppm. Vitamin C solutions as the positive control were prepared on similar various concentrations. The negative control was prepared using DPPH solutions dissolved in absolute ethanol. The results indicated that the ethanol extract of moringa leaves prepared by maceration method showed the antioxidant potency with an IC50 value of 22.1818 ppm, but the IC50 value of water extract of moringa leaves prepared by dekok was 57.5439 ppm. While, the IC50 value of Vitamin C was 8.8084 ppm. Based on the IC50 data it can be concluded that Vitamin C is a stronger antioxidant than moringa leaves extracts.

Isoprenylated Phenolic Compounds from Morus macroura as Potent Tyrosinase Inhibitors

Planta Medica ◽  
2017 ◽  
Vol 84 (05) ◽  
pp. 336-343 ◽  
Author(s):  
Yifan Wang ◽  
Liangjin Xu ◽  
Wen Gao ◽  
Lixin Niu ◽  
Chunyue Huang ◽  
...  
Keyword(s):  
Kojic Acid ◽  
Positive Control ◽  
Diels Alder ◽  
Extensive Analysis ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Tyrosinase Inhibitors ◽  
Inhibitory Activities ◽  
New Compounds

AbstractThree new Diels-Alder adducts, macrourins E – G (1–3), one new 2-arylbenzofuran, macrourin H (4), and eight known Diels-Alder adducts (5–12) were isolated from Morus macroura. Their structures were elucidated through extensive analysis of spectroscopic data. The 1H NMR and ECD trends in the determination of the configurations of these Diels-Alder adducts were summarized. The tyrosinase inhibitory activities of all compounds isolated were evaluated, and the new compounds (1–4) as well as the eight known compounds (5–12) were found to be potent with IC50 values ranging from 0.39 to 4.54 µM. Among them, 1 showed the best tyrosinase inhibitory activity with an IC50 value of 0.39 µM, approximately 50 times stronger than the positive control, kojic acid.

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Daun Alpukat Menggunakan Metode DPPH

2021 ◽  
Vol 11 (2) ◽  
pp. 104-110
Author(s):  
Alfin Surya ◽  
Zaiyar Nazir ◽  
Anggun Syazulfa
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Methanol Extract ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values

Free radicals are molecules that lose electrons in their outer orbitals so that the number of electrons becomes odd and unstable. Free radicals can damage various macromolecule cells including proteins, carbohydrates, fats. Antioxidants are compounds that can donate one or more electrons to free radicals. Avocado leaves contain high bioactive components that can be used as a source of natural antioxidants. Avocado leaves that have been dried, mashed and then shifted with methanol. The extracts were tested by phytochemical screening, including flavonpoid, phenolic, tannin and antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. This method is based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) against free radicals which causes a change from purple to yellow 2,2-diphenyl-1-picrylhydrazyl) (DPPH). From the research, the IC50 values ​​in avocado leaves were 118.8056 µg / mL and 7,276 µg / mL on ascorbic acid as a positive control. From the IC50 value obtained, it is known that the methanol extract of avocado leaves has strong antioxidant activity to fight free radicals.

scholarly journals Antioxidant Activity Test of Ethanol and Water Extracts of Celery (Apium graveolens L.)

2020 ◽  
Vol 9 (2) ◽  
pp. 93-101
Author(s):  
Nurmiati Nurmiati ◽  
Siti Nuryanti ◽  
Tahril Tahril
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Water Extract ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Apium Graveolens ◽  
Antioxidant Power ◽  
Water Extracts ◽  
Activity Test ◽  
Ic50 Value

This study aimed to determine the antioxidant activity of ethanol and water extracts of celery (Apium graveolens L.). Antioxidant activity test in this study used DPPH (2,2-diphenyl-1-picrylhydrazine) as a source of free radicals and vitamin C as a positive control. The antioxidant activity of celery extracts was analyzed using a UV-VIS spectrophotometer. Celery powder was extracted by firstly the maceration method using different solvents (ethanol and water) and secondly with the decoction method using water solvents. Celery extract was tested for antioxidant activity with various concentrations of 20, 40, 60, and 80 ppm. The results obtained that water extract (maceration) had a very strong antioxidant power with 23.713 ppm of IC50 values, for ethanol extract (maceration) had a strong antioxidant power with 59.492 ppm of IC50 value, water extract (dekok) had also a very strong antioxidant with 77.446 ppm of IC50 value, and vitamin C had a very strong antioxidant power with 15.631 ppm of an IC50 value. Based on the IC50 value obtained, the water extract by the maceration method was very good to be used as a source of natural antioxidants, because it had a very strong antioxidant activity value which was equivalent to vitamin C.

scholarly journals Phenolic Compounds from Carissa spinarum Are Characterized by Their Antioxidant, Anti-Inflammatory and Hepatoprotective Activities

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 652
Author(s):  
Ye Liu ◽  
Yongli Zhang ◽  
Felix Wambua Muema ◽  
Festus Kimutai ◽  
Guilin Chen ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Cell Injury ◽  
Positive Control ◽  
Root Bark ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ic50 Values ◽  
Cox 2 ◽  
Anti Inflammatory

Carissa spinarum has been traditionally used for the treatment of various diseases due to its different pharmacological activities. However, the active compounds responsible for its potentially specific activities have rarely been explored. To this end, the ethyl acetate (EA) fraction was screened out and selected for further phytochemical isolation because of its promising activities in preliminary 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and COX-2 inhibition assays. As a result, 10 compounds (1−10), including a new one (5), were isolated, with eight of these being identified as phenolic compounds, as expected. Compound 9 possessed an IC50 value of 16.5 ± 1.2 µM, which was lower than that of positive control (vitamin C, 25.5 ± 0.3 µM) in the DPPH assay, and compounds 2, 6, 7 and 9 showed better total antioxidant capacity than vitamin C in the FRAP assay. Meanwhile, compounds 1−6 and 9 also had IC50 values of less than 1.0 µM, which was even better than the positive control indomethacin in the COX-2 inhibition assay. In this context, compounds 2 and 9 were further evaluated to exhibit clear hepatoprotective activities by improving the L02 cell viability and reducing ROS production using a H2O2-induced L02 cell injury model. This study provides initial evidence revealing the most potent phenolic compounds from the root bark of C. spinarum responsible for its antioxidant, anti-inflammatory and hepatoprotective activities.

Inhibition of Protein Tyrosine Phosphatase 1B by Lutein Derivatives isolated from Mexican Oregano (Lippia graveolens)

2018 ◽  
Vol 17 (3) ◽  
pp. 134-139
Author(s):  
R.M. Perez-Gutierrez
Keyword(s):  
Protein Tyrosine Phosphatase ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Methanol Extract ◽  
Tyrosine Phosphatase ◽  
Positive Control ◽  
Protein Tyrosine Phosphatase 1B ◽  
Protein Tyrosine ◽  
Ic50 Value ◽  
Ic50 Values

Methanol extract from Lippia graveolens (Mexican oregano) was studied in order to identify inhibitory bioactives for protein tyrosine phosphatase 1B (PTP1B). Known flavone as lutein (1), and another flavone glycoside such as lutein-7-o-glucoside (2), 6-hydroxy-lutein-7-ohexoside (3) and lutein-7-o-ramnoide (4) were isolated from methanol extract of aerial parts of the Lippia graveolens. All isolates were identified based on extensive spectroscopic data analysis, including UV, IR, NMR, MS and compared with spectroscopic data previously reported. These flavones were evaluated for PTP1B inhibitory activity. Among them, compounds 1 and 3 displayed potential inhibitory activity against PTP1B with IC50 values of 7.01 ± 1.25 μg/ml and 18.4 μg/ml, respectively. In addition, compound 2 and 4 showed moderate inhibitory activity with an IC50 value of 23.8 ± 6.21 and 67.8 ± 5.80 μg/ml respectively. Among the four compounds, luteolin was found to be the most potent PTP1B inhibitor compared to the positive control ursolic acid, with an IC50 value of 8.12 ± 1.06 μg/ml. These results indicate that flavonoids constituents contained in Lippia graveolens can be considered as a natural source for the treatment of type 2 diabetes.

Sign in / Sign up

Export Citation Format

Share Document