scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Semangka (Citrullus lanatus)

2018 ◽  
Vol 7 (2) ◽  
pp. 96
Author(s):  
Sri Mariani ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Citrullus Lanatus ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Control Measurement ◽  
Ic50 Value ◽  
Ic50 Values

The aim of this study is determine the IC50 extracts values of white peel and flesh of red watermelon, white peel and flesh of yellow watermelon as antioxidants. To determine antioxidant activities DPPH reagent was used as well as UV-Vis Spectrophotometry was used to measure vitamin C as a positive control measurement. The samples mixed with ethanol in various concentration of 5, 15, 25 and 35 mg/L. The results showed that the IC50 values for each extract of red watermelon flesh, yellow watermelon flesh, white peel of red watermelon, and white peel of yellow watermelon were 16.619 mg/L, 16.575 mg/L, 14.729 mg/L, and 16.782 mg/L, respectively. Vitamin C as the positive control has IC50 value of 9.526 mg/L. These IC50 values showed that vitamin C had higher antioxidant activity than watermelon extracts, but the extracts of watermelon still categorized in strong natural antioxidants. This study concluded that watermelon is good to be consumed because it is a very strong antioxidant.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Semangka (Citrullus lanatus)

2018 ◽  
Vol 7 (3) ◽  
pp. 107
Author(s):  
Sri Mariani ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Citrullus Lanatus ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Control Measurement ◽  
Ic50 Value ◽  
Ic50 Values

The aim of this study is to determine the IC50 extracts values of white peel and flesh of red watermelon, white peel and flesh of yellow watermelon as antioxidants. To determine antioxidant activities DPPH reagent was used and UV-Vis spectrophotometry was used to measure Vitamin C as a positive control measurement. The samples mixed with ethanol in a various concentration of 5, 15, 25 and 35 mg/L. The results showed that the IC50 values for each extract of red watermelon flesh, yellow watermelon flesh, white peel of red watermelon, and white peel of yellow watermelon were 16.619, 16.575, 14.729, and 16.782 mg/L, respectively. Vitamin C as positive control has an IC50 value of 9.526 mg/L. These IC50 values showed that vitamin C had higher antioxidant activity than watermelon extracts, but the extracts of watermelon still categorized in strong natural antioxidants. This study concluded that watermelon is good to be consumed because it is a very strong antioxidant.

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Daun Alpukat Menggunakan Metode DPPH

2021 ◽  
Vol 11 (2) ◽  
pp. 104-110
Author(s):  
Alfin Surya ◽  
Zaiyar Nazir ◽  
Anggun Syazulfa
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Methanol Extract ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values

Free radicals are molecules that lose electrons in their outer orbitals so that the number of electrons becomes odd and unstable. Free radicals can damage various macromolecule cells including proteins, carbohydrates, fats. Antioxidants are compounds that can donate one or more electrons to free radicals. Avocado leaves contain high bioactive components that can be used as a source of natural antioxidants. Avocado leaves that have been dried, mashed and then shifted with methanol. The extracts were tested by phytochemical screening, including flavonpoid, phenolic, tannin and antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. This method is based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) against free radicals which causes a change from purple to yellow 2,2-diphenyl-1-picrylhydrazyl) (DPPH). From the research, the IC50 values ​​in avocado leaves were 118.8056 µg / mL and 7,276 µg / mL on ascorbic acid as a positive control. From the IC50 value obtained, it is known that the methanol extract of avocado leaves has strong antioxidant activity to fight free radicals.

scholarly journals Phytochemistry and Antioxidant Activities of the Methanolic Leaf Extract of Clerodendrum splendens (Lamiaceae)

2018 ◽  
pp. 12-20
Author(s):  
Nganso Ditchou Yves Oscar ◽  
Amang A Ngoung GA ◽  
Soh Desire ◽  
Simo Nemg Fredy Brice ◽  
Nyasse Barthelemy
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Maximum Activity ◽  
Hepatic Lipids ◽  
Ic50 Value ◽  
Ic50 Values

This paper aimed at studying the antioxidant efficacy of the methanolic leaf extract of Clerodendrum splendens, a plant of the Lamiaceae family. Phytochemical tests carried out on extracts of Clerodendrum splendens leaves have been able to detect the presence of secondary metabolites such as Flavonoids, Tannins, Saponins and Terpenoids. The results of the antioxidant activity have shown that CSF2, CSF3 fractions and CSB, CSG fractions similarly inhibited hepatic lipids but significantly less than vitamin C. Compared to all fractions, the CSB fraction shows the best inhibitor on the peroxidation of hepatic lipids because at 150 μg/mL, there is a maximum activity (2.5 μg/mL of protein). However, it is found that CSF3, CSF2 and CSG have higher IC50 values than vitamin C (5.613±0.117) while CSEB, CSB and CSC fractions showed lower IC50 values than vitamin C, which is used as the reference reducing compound. The lower the IC50 value compared to vitamin C, the greater the antioxidant capacity of the plant extract. The results of this study suggest that Clerodendrum splendens represents an untapped source of compounds with potential antioxidant activity that could be explored in the development of new therapeutic natural products.

scholarly journals Antioxidant Activity Tests of Extract of Principle Crafts (Phaleria macrocarpa)

2020 ◽  
Vol 8 (3) ◽  
pp. 125-129
Author(s):  
Tri Putri ◽  
Anang W. M. Diah ◽  
Afadil Afadil
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Positive Control ◽  
Degenerative Diseases ◽  
Fruit Extract ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Phaleria Macrocarpa ◽  
Central Sulawesi

Phaleria macrocarpa contains secondary metabolite compounds such as alkaloids, terpenoids, saponins, polyphenols, tannins, sterols, and coumarins. Those metabolites are antioxidants and can be efficacious in the healing of various degenerative diseases such as cervical cancer and diabetes. This plant is widely grown in Tindaki Village, Parigi Mautong, Central Sulawesi. The aim of this study was to determine the antioxidant power of those phaleria macrocarpa fruit extract. This study was conducted by using maceration extraction technique with ethanol as solvent, and compound 2,2-difenil-1-picrilhidrazil (DPPH) as source of free radical, and vitamin C as the positive control. The UV-Vis spectrophotometer was used to measure the absorbance of the extract. Various concentrations of the extract used were 5 ppm, 15 ppm, 25 ppm, and 35 ppm. The results showed that the IC50 value of the fruit extract was 28.242 ppm, while the IC50 value of vitamin C was 19.302 ppm. These IC50 values show that vitamin C was a stronger antioxidant activity than the fruit extract, but the fruit extract was still as a very strong natural antioxidant category.

scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN KRIM EKSTRAK ETANOL DAUN MURBEI (Morus alba L.) MENGGUNAKAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 349
Author(s):  
Eklesia Pogaga ◽  
Paulina V. Y. Yamlean ◽  
Julianri S. Lebang
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Traditional Medicine ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Mulberry Leaves ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Ethanol Extracts

ABSTRACTMulberry is one of the plants growing in Indonesian and widely used as traditional medicine. The active compounds found in Mulberry leaves are known have an antioxidant activity. The aim of this study is to formulate cream preparations from ethanol extracts of Mulberry leaves and to determine its antioxidant activities. Mulberry leaves was macerated using ethanol 96%. Antioxidant activity of mulberry leaves cream then evaluated against DPPH as free radical and measured at 517 nm wavelength using a UV-Vis spectrophotometer. The results showed that the cream of ethanol extract from Mulberry leaves had a very strong antioxidant activity with IC50 values in a row that is 1.7831 ppm, 0.8215 ppm and 0.7668 ppm. This can be compared with a Vitamin C solution, which has a very strong antioxidant activity with IC50 value was 1.1113 ppm. This study concludes that the cream from Mulberry leaves ethanol extract has a very strong antioxidant activity.Keywords: Mulberry leaves, Cream, Antioxidant, DPPHABSTRAK Murbei merupakan salah satu tanaman yang tumbuh di Indonesia dan  banyak digunakan dalam pengobatan secara tradisional. Kandungan senyawa aktif yang terdapat pada daun Murbei dikenal mempunyai peranan sebagai antioksidan. Tujuan dari penelitian ini ialah untuk membuat sediaan krim dari ekstrak etanol daun Murbei dan untuk menguji apakah adanya aktivitas antioksidan. Daun murbei diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Sediaan krim diuji terhadap DPPH sebagai radikal bebas dan diukur pada panjang gelombang 517 nm  dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa krim ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat  dengan  nilai IC50 berturut-turut yaitu 1,7831 ppm, 0,8215 ppm dan 0,7668 ppm. Hal ini dapat dibandingkan dengan larutan Vitamin C yang mempunyai aktivitas antioksidan yang sangat kuat, yaitu 1.1113 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa krim dari ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Daun Murbei, Krim, Antioksidan, DPPH

scholarly journals Uji Aktivitas Antioksidan Ekstrak Etanol Biji Buah Merah (Pandanus Conoideus De Vriese) Asal Kabupaten Poso Sulawesi Tengah

2018 ◽  
Vol 6 (3) ◽  
pp. 170
Author(s):  
Siti Sulaeha ◽  
Minarni R. Jura ◽  
Nurdin Rahman
Keyword(s):  
Vitamin C ◽  
Scientific Information ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Ic50 Values

Red fruit seeds have not been studied and known benefits, therefore it needed scientific information about the content and benefits of red fruit seeds. This study aims to analyze the antioxidant power red fruit seed extract as well determining the IC50 value of red fruit seed extract which has the ability of natural antioxidants. Determination of free radical DPPH (1,1-diphenyl-2-picrylhidrazyl) after the addition of red fruit seeds extract is determined using UV-Vis spectrophotometer. Variations in the concentration of the red fruit seed extract used is 20 ppm, 40 ppm, 60 ppm, 80 ppm and 100 ppm.The positive control used is vitamin C with the same concentration. While the negative control using DPPH solution dissolved in absolute ethanol. The results showed IC50 values obtained for the red fruit seed extract and vitamin C are 223.8 ppm and 1.73 ppm. Based on the IC50 data can be known that vitamin C antioxidants more powerful than red fruit seed extract which belongs to natural antioxidants are very weak.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN BEBERAPA EKSTRAK ALGA COKLAT Sargassum polycystum DAN Turbinaria deccurens ASAL PULAU DUTUNGAN SULAWESI SELATAN TERHADAP RADIKAL DPPH

2019 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Fitriyanti Jumaetri Sami ◽  
Nunuk Hariani Soekamto ◽  
Firdaus Firdaus ◽  
Jalifah Latip
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Vitamin ◽  
Activity Assay ◽  
Immersion Method ◽  
Ic50 Value ◽  
Measured Absorption ◽  
Hexane Extracts

This study used S. polycystum and T. deccurens were extracted with various solvents (n-hexane, ethyl acetate and ethanol) to determine phytochemical screening and antioxidant activities. Extraction of samples by maceration method, antioxidant activity assay using the immersion method of DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 515 nm and compared with the control of antioxidant vitamin C. The results showed that antioxidant activity of the methanol, ethyl acetate, n-hexane extract of S. polycystum have low antioxidant activity with the value IC50 340,06, 180,54, and 502,25 mg/mL. Methanol, ethyl acetate, n-hexane extracts of  T. deccurens have low antioxidant activity with value IC50 491,02, 411,80, and 502,7 mg/mL. This potential is lower than vitamin C IC50 value of 1,72 mg/mL. 

scholarly journals ANTIOXIDANT ACTIVITIES OF HYDROLYSATES ORIGINATED FROM SOYBEAN AND SOY MILK RESIDUE

2018 ◽  
Vol 55 (5A) ◽  
pp. 134
Author(s):  
Ngo Minh Ngoc
Keyword(s):  
Antioxidant Activity ◽  
Protein Content ◽  
Manufacturing Industry ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging ◽  
Soy Milk ◽  
Dpph Radical ◽  
Ic50 Value ◽  
Ic50 Values

Soybean (Glycine max) and soy milk residue (okara) are protein-rich materials. Soybean possesses the highest protein content among different types of beans (protein content of soybean varies from 40–42 %). Soy milk residue, a by-product of the soy milk manufacturing industry, contains approximately 27 % protein (by dry weight). A number of recent studies have investigated the improvement of functional properties of protein contained in soybean and okara by fermentation or by the use of proteolytic enzymes. The aim of this study was to evaluate the antioxidant activities of soybean and okara hydrolysates obtained by the fermentation with Aspergillus oryzae or by using proteolytic enzymes (neutrase and flavourzyme). DPPH radical scavenging assay was used to determine the antioxidant activities of hydrolysates. The concentration of peptides required to scavenge DPPH radical by 50 % (IC50 value) was used to evaluate the antioxidant activity of peptides produced obtained from hydrolysates. The results showed that when fermented with A. oryzae, the okara hydrolysate had higher antioxidant activity than the soybean hydrolysate, with IC50 values of 0.447 mg/ml and 3.95 mg/ml, respectively. The hydrolyzed okara obtained from hydrolysis using Neutrase had higher antioxidant activity than the one obtained from hydrolysis using Flavourzyme, with IC50 values of0.200 mg/ml and 0.407 mg/ml, respectively. Different peptide fractions obtained from the hydrolysates using cut-off membrane (10 kDa, 3 kDa and 1 kDa) possessed different antioxidant activities. The < 1 kDa peptide fraction exhibited the highest antioxidant activity with an IC50 value of 0.158 mg/ml.

scholarly journals Uji Aktivitas Antioksidan Hidrolisat Protein Kerang Pasir (Semele cordiformis) dengan Metode DPPH (Antioxidant Activity of Protein Hydrolisate from Semele cordiformis Using DPPH Methode)

MEDULA ◽  
2020 ◽  
Vol 7 (2) ◽  
Author(s):  
Ummi Kalsum ◽  
Indria Hafizah ◽  
Pranita Aritrina ◽  
Sulastrianah Sulastrianah
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Protein Hydrolysate ◽  
Protein S ◽  
Positive Control ◽  
Control Group ◽  
Control Group Design ◽  
Ic50 Value ◽  
Dpph Method ◽  
Post Test

Background: Sand shell (Semele cordiformis) is commonly found in South East Sulawesi marine area. This species traditionally used for treatment of several disease. Nevertheless, there is less publication about bioactivity of S. cordiformis as asource of drugs. Purpose: The aims of this research is to determine the activity of protein hydrolysate of S. cordiformis as an antioxidant. Methods: This research was conducted by pre-experimetal study with post test only control group design. Antioxidant activity was measure by DPPH method and spectrophotometry. The sample was hydrolyzed by using papain enzyme. The sample was made in to concentration 200 ppm, 400 ppm, 600 ppm, 800 ppm, and 1000 ppm and vitamin C was used as positive control. The IC50 was used to measure antioxidant activity. Results: Concentration of 200 ppm showed 36,68% of inhibition percentage, 400 ppm showed 47,69%, 600 ppm showed 60,19%, 800 ppm showed 65,63%, and 1000 ppm showed 72,69 %. The IC50 value of was obtained for 453,777 ppm. Conclusion: The protein hydrolysate of S. cordiformis have an antioxidant activity and the hydrolisat concentration is directly proportional with inhibition activity.Keyword: antioxidant, protein hydrolysate, Semele cordiformis ABSTRAKPendahuluan: Kerang pasir atau Semele ccordiformis merupakan jenis kerang yang banyak ditemukan di perairan Sulawesi Tenggara. Kerang ini merupakah salah satu jenis kerang yang telah dimanfaatkan secara turun-temurun oleh masyarakat lokal untuk pengobatan. Meskipun demikian, belum ditemukan publikasi ilmiah mengenai bioaktifitas S. ccordiformis dalam bidang kesehatan. Tujuan: Penelitian ini bertujuan untuk mengetahui aktifitas antioksidan hidrolisat protein S. cordiformis. Metode: Penelitian menggunakan desain pra-eksperimental dengan pendekatan post-test only control group. Uji aktivitas antioksidan menggunakan metode DPPH dengan prinsip spektrofotometri. Sampel dari penelitian ini adalah S. cordiformis yang diperoleh dari perairan Pulau Bokori, Kabupaten Konawe, Provinsi Sulawesi Tenggara yang kemudian dihidrolisis menggunakan enzim papain. Sampel dibuat menjadi beberapa konsentrasi yaitu 200 ppm, 400 ppm, 600 ppm, 800 ppm, and 1000 ppm dan vitamin C digunakan sebagai kontrol positif. Nilai IC50 digunakan untuk mengetahui aktifitas antioksidan. Hasil: Konsentrasi 200 ppm menghasilkan persen inhibisi sebesar 36,68%, 400 ppm sebesar 47,69%, 600 ppm sebesar 60,19%, 800 ppm sebesar 65,63%, dan 1000 ppm sebesar 72,69 %. Nilai IC50 yang diperoleh adalah 453,777 ppm. Simpulan: hidrolisat protein S. cordiformis memiliki aktivitas antioksidan dengan konsentrasi hidrolisat berbanding lurus terhadap aktifitas inhibisi.Kata Kunci: antioksidan, hidrolisat protein, Semele cordiformis

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