scholarly journals Effects of tissue neurotransplantation on sceletal muscle tone restoration after experimental mechanical injury of the cerebellum

2013 ◽  
Vol 1 (1) ◽  
pp. 81-86
Author(s):  
V. Tsymbalyuk ◽  
V. Medvediev ◽  
Yu. Senchyk
Keyword(s):  
Muscle Tone ◽  
Study Groups ◽  
Cerebellar Hemisphere ◽  
Control Group ◽  
Fast Recovery ◽  
Slow Recovery ◽  
Adult Rats ◽  
Slow Increase ◽  
Walking Test ◽  
Muscle Hypotonia

This work aimed to conduct a comparative study of the restorative effects of transplantation of fetal neural tissue (FNT), olfactory bulb tissue (OBT) and fetal kidney (FK) on the dynamics of muscle hypotonia after cerebellar hemisphere injury in the adult rats. Beam walking test (BWT) allowed detect at least three degrees of hypotonia which correspond to 2, 3, and 4 points. The authors selected animals with function index (FI) by BWT scale strictly lesser than 4 points on the 3rd day after injury. Moderate hypotonia was associated with FI 3 points, severe – 2 points, and mild-4 points. Major differences in the dynamics of the restorative process across study groups were detected at the first month of study: slow recovery of statics and coordination (control); fast recovery (during first 9 days, FK, OBT and FNT groups) that underwent changes by its slow increase during 9th–33rd day. Mild hypotonia in the control group showed itself by the end of the 1st month and on the 9th day in the FK, OBT and FNT groups. Normotony was observed on the 21st (group FNT) and 30th day (groups FK and OBT). These data suggest that neurotransplantation has a significant effect on muscle tone improvement after cerebellar injury, depending on the type of graft.

scholarly journals SUN-076 Somatic and Neurodevelopmental Outcome and Muscle Tone in 5 to 9 Year Old Children Born After Intracytoplasmic Sperm Injection

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Marta Snajderova ◽  
Josef Kraus ◽  
Daniela Zemkova ◽  
Tonko Mardesic
Keyword(s):  
Motor Development ◽  
Intracytoplasmic Sperm Injection ◽  
Muscle Tone ◽  
Neurodevelopmental Outcome ◽  
Control Group ◽  
Special Focus ◽  
Proximal Muscle ◽  
Neurological Development ◽  
Muscle Hypotonia ◽  
Girdle Muscle

Abstract Introduction: Assessing the development and health status of children born after assisted reproductive techniques is very important. This also applies to somatic and neurological development. Little is known on the development of muscle tone in children. Aim of our study was to evaluate the somatic and neurological development in children born after intracytoplasmic sperm injection (ICSI) with special focus on proximal muscle tone. Material and methods: A group of 82 singletons (ICSI) aged 5–9 years (42 M, 40 F) and a control group of 82 singletons spontaneously conceived (SC), all with low morbidity, were compared by age and sex. Comprehensive assessment by endocrinologist, clinical anthropologist and pediatric neurologist was performed. Results: Both ICSI and SC children had normal somatic development. In the standard neurological testing, motor development did not differ significantly in ICSI children compared with the general population. Nevertheless, some coordination abnormalities tested by diadochokinesis and by the finger-nose test, were found in all but 7 ICSI children (ICSI in 91 % versus SC in 9 %; p<0.001). A prominent hypotonia of upper girdle muscles tested by the scarf sign was found in all but 4 ICSI children (ICSI in 95 % versus SC in 61 %; p<0.001). In the contrary, no difference was found for lower girdle muscle tone in ICSI versus SC children. Any of the factors tested for possible relationship to upper girdle muscle hypotonia was not found to be significant. Conclusions: As far as we know, this study is the first evaluation of proximal muscle tone in ICSI children aged 5 - 9 years. Subtle changes in the neurological status were revealed comparing ICSI and SC children, i. e. the prominent upper girdle muscle hypotonia and the coordination changes. The hypotonia can be explained by a slight change in the muscle tone maturation and movement coordination. The ICSI method very likely does not have any negative effect on the neurodevelopmental outcome of children. Nevertheless, the development of muscle tone and coordination in ICSI children should be monitored. Early diagnosis of these abnormalities helps to early initiation of appropriate therapy and thus avoids possible complications.

COGNITIVE DISABILITY IN ESTROGENECTOMITED AND OLD RATS WITH DEVELOPMENT OF DIABETES MELLITUS

2019 ◽  
Vol 72 (8) ◽  
pp. 1517-1522
Author(s):  
Inga R. Tymofiychuk ◽  
Ihor K. Churpiy ◽  
Tetiana P. Savchuk ◽  
Lilia D. Boreyko ◽  
Xenia V. Slobodian
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Spatial Memory ◽  
Study Groups ◽  
Control Group ◽  
Cognitive Disability ◽  
Adult Rats ◽  
Experimental Type ◽  
Old Rats

Introduction: In recent years, there have been many works devoted to the study of the effects of sex hormones on cognitive function. Clinical studies have found that in menopausal women, the tendency to develop type 2 diabetes will increase, the spatial and short-term working memory worsens, and there is a tendency to develop depression. The findings suggest that estrogens are involved in the mechanisms of insulin resistance in tissues, in the synthesis of mediators in the catecholaminergic systems of the brain, but many questions remain unresolved. The aim: Therefore, the aim of our study was to establish the effect of estrogens on the indices of spatial memory in ovariectomized and old rats against the background of the development of experimental diabetes. Materials and methods: The study was conducted on 30 adult rats females 4-5 months and 20 months. The study groups were ovariectomized and reproduced experimental type 2 diabetes mellitus with protamine sulfate. The study of spatial memory was carried out in an eight-sleeved radial labyrinth. Results and conclusions: Ovariectomy caused the deterioration of spatial memory relative to the living control group, and diabetes mellitus aggravated pathological changes. The changes that occur after ovariectomy suggest estrogen involvement in the regulation of cognitive functions.

scholarly journals Efficacy of Intratracheal Budesonide-Surfactant Combined Therapy in Surfactant-Insufficient Rat Lungs with Lipopolysaccharide Insult

2021 ◽  
Author(s):  
Pei-Chen Tsao ◽  
Chih-Hsueh Lin ◽  
Yu-Sheng Lee ◽  
Wei-Yu Chen ◽  
Mei-Jy Jeng ◽  
...  
Keyword(s):  
Lung Injury ◽  
Combined Therapy ◽  
Study Groups ◽  
Control Group ◽  
Post Injury ◽  
Adult Rats ◽  
Male Adult ◽  
Full Strength ◽  
Rat Lungs ◽  
And Control

Objectives: Intratracheal steroid therapy for lipopolysaccharide (LPS)-induced acute lung injury (ALI) remains challenging particularly in surfactant-deficiency lungs, a common problem of preterm infants. Surfactant has been used as a vehicle for intratracheal steroid in the treatment of other types of ALI. This study investigated the efficacy of intratracheal budesonide (BUD) delivered by two concentrations of surfactant in the treatment of LPS-induced ALI in surfactant-insufficiency rat lungs. Methods: Male adult rats were anesthetized and ventilated. Our ALI model was established by repeated saline lavage to produce surfactant insufficiency, followed by intratracheal LPS instillation. Five study groups (n=5 for each) with different intratracheal treatments following ALI were used: Control (no treatment), BUD (IT-NS-BUD; BUD in saline); IT-DS-BUD (BUD in diluted surfactant); IT-FS-BUD (BUD in full-strength surfactant); IT-FS (full-strength surfactant). Cardiopulmonary variables were monitored 4 h post injury. Histological and immunohistochemical assessments of the lungs were performed. Results: The IT-FS-BUD and IT-FS groups presented better gas exchange, less metabolic acidosis, less oxygen index, and more stable hemodynamic changes than the IT-DS-BUD, IT-NS-BUD, and Control groups. The total lung injury scores assessed by histological examination were ordered as follows: IT-FS-BUD < IT-DS-BUD or IT-FS < IT-NS-BUD < Control. The immunostaining intensities of lung myeloperoxidase showed the following order: IT-NS-BUD, IT-DS-BUD, or IT-FS-BUD < Control or IT-FS. Only the IT-FS-BUD group displayed a smaller immunostaining intensity of lung TNF-α than the control group. Conclusion: Among our therapeutic strategies, intratracheal BUD delivered by full-strength surfactant confers an optimal protection against LPS-induced ALI in surfactant-insufficiency rat lungs.

Sucrose and Facilitated Tucking for Pain Among Neonates Receiving Vaccination, in Puducherry

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Sujatha S. ◽  
Rebecca Samson ◽  
Christopher Amalraj ◽  
Sundaresan Sundaresan
Keyword(s):  
Sucrose Solution ◽  
Study Groups ◽  
Primary Outcome Measure ◽  
Intradermal Injection ◽  
Control Group ◽  
Intradermal Vaccination ◽  
Term Neonates ◽  
Bcg Vaccination ◽  
Pharmacologic Agents ◽  
Study Intervention

Neglected pain in neonates leads to various ill effects and it can be prevented by using simple and safe non-pharmacological pain relieving measures. Pharmacologic agents are not recommended in neonates for acute pain due toinvasive procedures however, administration of 24% oralsucrose solutionis found to be effective. The objective of this study was to assess the efficacy of 24%oral sucrose in combination with Facilitated tucking during BCG Vaccination through intradermalroute in term neonates which is not done elsewhere. Fifty five healthy term neonates who fulfilled the inclusion criteria such as gestational age above 37 weeks, within 24 hoursof birth age, and neonates delivered only through spontaneous vaginal delivery were included in the study. The study intervention consists of administration of 2 ml of oral 24% sucrose 2 minutes before BCG Vaccination through intradermal route and Facilitated tuckingat the time of vaccination. The primary outcome measure of cumulative NIPS score at 0, 3,5 minuteswas not significant in both the study groups. Whereas there was significant reduction in the level of pain and mean cry time in the neonates of sucrose group. Heart rateand oxygen saturation after intradermal injection also showed significant (p less than 0.001) differenceamong the neonates, who received 24% of oral sucroseand Facilitated tucking than for neonates of control group. Thus oral (24%)sucrose solution given 2 minutes before injection was effective in reducing level of neonatal pain following Intradermal Vaccination. It is a simple, safe and fast acting analgesic and should be considered for minor invasive procedures in term neonates which last for 5-7minutes.

Employing Gamma Ray Irradiated Giardia lamblia as Trialed Vaccine in Experimental Animal

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Amal Kamil Abdul Sada ◽  
Amany Mohamed Al-Kaysi
Keyword(s):  
Giardia Lamblia ◽  
Gamma Ray ◽  
Age Groups ◽  
Active Phase ◽  
Study Groups ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Histopathological Changes ◽  
Gamma Irradiated

This is an experimental trial to prepare a vaccine from gamma-irradiated Giardia lamblia which is evaluated in experimental animals. The study was conducted from December 2015 to April 2016. The field survey of the parasite was conducted from those patients attending the laboratories of the Alawi Children's Hospital in Rusafa and the Al-Yarmouk Teaching Hospital in Karkh, through which 1250 stool samples of different age groups were examined. Five groups of mice were used in the study; the first was injected with normal saline and considered as a negative control group, the second was injected with cystic form of non-irradiated Giardia lamblia and considered as a positive control group, whereas the other three groups were injected with gamma irradiated Giardia lamblia at three different doses 10, 15 and 25 rad respectively. Giardia lamblia was primarily cultivated in liver infusion agar for ten days to obtain the active phase. On the sixth day, the cystic phase was purified and standardized to be used in the infection of mice with or without the exposure of gamma rays. Mice showed high sensitivity to parasitic infestation, in the gamma non-irradiated and the irradiated with gamma 10 rad, and 15 rad irradiated groups which was 100%. The results expressed an excystation process of the depleted phases and the release of the feeder phases. The results of the three irradiated groups consisted of histopathological changes of the small, and the rectum by dissection after two weeks of infection, with intestine amputation lesions, as well as ulceration and inflammation of the inflammatory cells represented in small numbers of neutrophil, lymphocytes, and eosinophils. The presence of ulceration and fall of epithelial cells in the intestinal cavity has been shown, and different forms of the parasite have been observed. Mice which was injected with irradiated G lamblia at high dose (25 rad), not show and sensitivity to the challenge infection and no excystation of thy parasite had been done. After 2 wreaks, a comparison was achieved between all study groups in which no histopathological changes were noticed in the mice irradiated with dose of25 rad. After another two weeks, a challenge dose was given (un-attenuated G lamblia) and mice were dissected after another two weeks, no changes on the level of histopathology of intestinal tissue were noticed the results suggested that mice acquire an immunity against the parasite infection.

A STUDY ON THE VARIATION IN CA 72-4 LEVELS OF THE GASTRIC CANCER’S PATIENTS BEFORE AND AFTER SURGERY TREATMENT

2011 ◽  
pp. 81-87
Author(s):  
Thi Thu Huong Hoang ◽  
Minh Vuong Nguyen
Keyword(s):  
Gastric Cancer ◽  
Study Groups ◽  
University Hospital ◽  
Control Group ◽  
Central Hospital ◽  
Before And After

Objectives: Studying on the variation in CA 72-4 levels of the gastric cancer’s patients before and after 10 days and 30 days surgery treatment. Materials and methods: The studying group included 42 gastric cancer’s patients who were examinated and treated in cancerology service of Hue University Hospital and gastroenterology service of Hue Central Hospital. The control group included 30 healthy normal examinated at Hue University Hospital. The study groups were clinical, endoscopic anatopathologic examination diagnosed with gastric cancer and quantitative levels of CA 72-4 in three times points: before surgerying, after surgerying 10 days and 30 days postoperatively. Rerults: The concentration of CA 72-4 in gastric cancer’s patients was 10.06 ± 16.49 U/ml. Clearly higher than the control group 1.2 ± 0.4 U/ml(p <0.01). The rate increased levels of CA 72-4 in gastric cancer’s patients before surgerying was 27.5% and the control group was 0%. After 10 days of surgery, CA 72-4 level was 5.56 ± 8.55 U/ml; 82.5% of patients have reduced levels of CA 72-4 and 17.5% no changes; there are 0% increased cases. After 30 days of surgery, CA 72-4 level was 3.79 ± 6,52 U/ml. CA 72-4 level 10 days after surgering have decreased significantly compared to before surgery (p < 0.05) and 30 days after surgery have decreased significantly compared to after 10 days (p < 0.05). 30 days postoperatively, 90% patients had reduced levels of CA 72-4, 10% no changes, no patient had increased levels of CA 72-4 and no patient be relapsed after 30 days of treatment. Conclusions: CA 72-4 concentrations before surgerying increased 27.5%, after surgery 10 days and 30 days reduced step by step, no case have increased CA 72-4 levels, no case relapsed after 30 days.

Results of Applying the Method of Visual Fatigue Prevention Using Low-Intensity Laser Radiation

2020 ◽  
pp. 56-59
Author(s):  
NYu Mal’kova ◽  
MD Petrova
Keyword(s):  
Laser Radiation ◽  
Work Experience ◽  
Study Groups ◽  
Light Sensitivity ◽  
Control Group ◽  
Sensitivity Threshold ◽  
Visual Fatigue ◽  
Strain Relief ◽  
Visual Stress ◽  
Stress At Work

Summary. Introduction: Visual fatigue is caused by changes in the muscular apparatus and retina of the eye and is characterized by deterioration in their functional activity. Along with an increase in work experience and age, workplace visual stress promotes the loss of performance. Known methods of visual fatigue prevention and eye strain relief are ineffective. In this regard, a technique of relieving visual fatigue using scattered low-level red laser radiation was developed and patented. The purpose of our study was a retrospective evaluation of effectiveness of the visual fatigue reduction technique. Materials and methods: We examined two groups of women (PC operators and jewellers) aged 43–57 years experiencing visual stress at work and practicing the method under study over the previous 20 years. The state of daylight vision was assessed by adaptation time. Results: A five-day testing of the method of visual fatigue relief showed stabilization of the light sensitivity threshold throughout the work shift. Over the 20-year period, the female workers had had no complaints of eye strain, burning or lacrimation. Objective studies of the functional state of the organ of sight showed that the light sensitivity threshold during the working day in two study groups was significantly lower than that in the control group with no preventive measures taken. Conclusions: We established that using the technique of eye strain relief based on a two-minute binocular exposure to red laser radiation with the power density of 2×10–7 W/cm2, a 5-day course every six months over the period of 20 years, helped retain retinal activity in terms of light sensitivity, thus preventing visual fatigue.

Effects Of 10,000 Steps Walking Program In Cardiorespiratory Endurance Of Obese Adolescent

2017 ◽  
Vol 6 (02) ◽  
pp. 35
Author(s):  
William Jakatama ◽  
Sri Wahyudati
Keyword(s):  
High School ◽  
High School Students ◽  
Human Life ◽  
Intervention Group ◽  
Control Group ◽  
Obese Adolescent ◽  
Walking Test ◽  
Walking Program ◽  
Walking Activity ◽  
Cardiorespiratory Endurance

Background: Sedentary life style lead to obesity which related into deterioration of cardiac function, anddeconditioning on musculoskeletal system, particularly on walking function. Walking is an important activityin human life, that automatically repeated in daily activity. Doing exercise by walking activity is a simple andsafe exercise. However, in walking exercise prescription, we need to establish what is the objective to achieve.The ten thousand steps of Walking Program (10,000 SWP) is pedometer-based walking program begin in Japanfor adult and elderly, thus the effects in obesity adolescent remains unclear. The aimed of this study was to findthe effect of 10,000 SWP in Cardiorespiratory Endurance (CE) on obese adolescent.Methods:This study was pre and post randomly experimental design with control, in 24 high school obeseadolescent. Subjects divided into two groups, the intervention group that received 10,000 steps walking program5 days in a week for 6 weeks, and the control group that number of step walking recorded by a pedometerwithout daily target. The cardiorespiratory endurance (VO2 max) was evaluated by the six minute walking test(6MWT), that measured before and after intervention.Results: The Intervention and control groups each contain 12 obese high school students, that equal in age,body mass index, and the mean number of walking steps per day. There was no differences between VO2max1 (12.45 ml/Kg ) and VO2max 2 (12.38 ml/Kg ) (p=0.852) in control group, while there was the differencesbetween VO2max 1 (12.44 ml/kg) and VO2max 2 (17.06 ml/kg) (p=0.002) in treatment group.Conclusion: The 10,000 SWP has proven increasing the Cardiorespiratory Endurance of Obese Adolescent.Keywords: 10,000 Steps Walking Program, 6 Minute Walking Test, Cardiorespiratory Endurance, Obese Adolescent.

Success Rate of Obturation of Root Canals by Different Techniques in Primary Molars: A Comparative Clinical Study

2020 ◽  
Vol 2 (1) ◽  
pp. 36-44
Author(s):  
Satyawan G. Damle ◽  
Ritika Bansal ◽  
Dhanashree D. Sakhare
Keyword(s):  
Success Rate ◽  
Study Groups ◽  
Primary Molars ◽  
Control Group ◽  
Second Primary ◽  
Chi Square ◽  
Exact Test ◽  
Root Canals ◽  
Significant Difference ◽  
Comparative Clinical Study

Objective: To compare the success rate of different obturation procedures in primary mandibular second molars clinically and also by digital radiovisiography. Methods: A total of 40 children aged between 4-8 years with deeply carious mandibular second primary molars indicated for single session pulpectomy were selected. Canals were obturated with Metapex. The 3 study groups (Endodontic plugger, Handheld lentulospiral, Navi Tip syringe) were compared with the control group (reamer) both clinically and radiovisiographically. The data collected were statistically analyzed using Pearson’s Chi-square and Fisher’s exact test. Results: The use of Navi tip syringe led to the least number of voids followed by Endodontic plugger and Reamer and the highest number of voids was reported with Lentulospiral. Navitip presented maximum number of optimally filled cases followed by Endodontic plugger and Lentulospiral and least number of optimally filled cases with reamer. However, there was no statistically significant difference (p>0.05) in any of the groups with clinical (pain and tenderness to percussion) and radiographic parameters (presence or absence of voids and length of obturation). Conclusion: Within the limitations of the present study, though the clinical outcome was statistically insignificant, Navitip syringe exhibited encouraging results and is a promising option for obturation in primary teeth.

scholarly journals Moderate Consumption of Beer (with and without Ethanol) and Menopausal Symptoms: Results from a Parallel Clinical Trial in Postmenopausal Women

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2278
Author(s):  
Marta Trius-Soler ◽  
María Marhuenda-Muñoz ◽  
Emily P. Laveriano-Santos ◽  
Miriam Martínez-Huélamo ◽  
Gemma Sasot ◽  
...  
Keyword(s):  
Postmenopausal Women ◽  
Study Groups ◽  
Menopausal Symptoms ◽  
Control Group ◽  
P Value ◽  
Intervention Trial ◽  
Hormone Profile ◽  
Perimenopausal Symptoms ◽  
Main Food Source

The menopausal transition can be a challenging period for women’s health and a trigger of uncomfortable symptoms. Beer is the main food source of isoxanthohumol, a precursor of 8-prenylnaringenin, the strongest phytoestrogen identified to date. As phytoestrogens are reported to reduce perimenopausal symptoms, we evaluated if a daily moderate consumption of beer with (AB) and without alcohol (NAB) could improve menopausal symptoms and modify cardiovascular risk factors. A total of 37 postmenopausal women were enrolled in a parallel controlled intervention trial and assigned to three study groups: 16 were administered AB (330 mL/day), 7 NAB (660 mL/day), and 14 were in the control group. After a 6-month follow-up of the 34 participants who finished the trial, both interventions (AB and NAB) significantly reduced the severity of the menopause-related symptoms (p-value AB vs. Control: 0.009; p-value NAB vs. Control: 0.033). Moreover, AB had a beneficial net effect on psychological menopausal discomforts compared to the control group. As the sex hormone profile did not differ significantly between the study groups, the effects of both types of beers (AB and NAB) are attributed to the non-alcoholic fraction of beer. Furthermore, moderate NAB consumption improved the lipid profile and decreased blood pressure in postmenopausal women.

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