scholarly journals Chlorophyll and carotenoid content in wheat cultivars as a function of mineral nutrition

2005 ◽  
Vol 57 (4) ◽  
pp. 283-290 ◽  
Author(s):  
Biljana Bojovic ◽  
J. Stojanovic
Keyword(s):  
Indirect Method ◽  
Mineral Elements ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Wheat Cultivars ◽  
Chl A ◽  
The One ◽  
Uppermost Internode

Determination of chlorophyll content as an indirect method of estimating the productivity of vegetation represents a good way to gain an understanding of the photosynthetic regime of plants. Physiological investigations of chlorophyll and carotenoid content in the uppermost internode of several wheat cultivars were carried out at the outset of the flowering phase. The dependence of chlorophyll and carotenoid content on the fertilization variant was established at that time. The tested wheat cultivars were grown under conditions of five fertilization variants. The content of chlorophyll a, chlorophyll b, and total chlorophyll (Chl a+b) was measured and carotenoid content was determined on each variant. The results indicate that chlorophyll and carotenoid content depended on the presence and ratio of mineral elements in the substrate. This is demonstrated by the variant with unfertilized soil, where chlorophyll and carotenoid content in all cultivars was lowest. The variant of fertilization with N and P turned out to be most favorable. The next most favorable variant was the one with nitrogen alone, and it was followed by the variant with N and K.

scholarly journals The Qualitative Differences for Photosynthetic Content of Common Bean (Phaseolus vulgaris L.) Populations  in Kosovo

2014 ◽  
Vol 6 (3) ◽  
pp. 331-334
Author(s):  
Sali Ali ALIU ◽  
Imer RUSINOVCI ◽  
Shukri FETAHU ◽  
Bekim GASHI
Keyword(s):  
Common Bean ◽  
Chlorophyll A ◽  
Room Temperature ◽  
Genetic Material ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Breeding Programs ◽  
Chl A ◽  
Acetone Water

Genetic diversity analysis of common bean populations is useful for breeding programs, as it helps to select genetic material to be used for further crossings. Twenty (20) common bean populations were analyzed using qualitative traits, chlorophyll “a” (Chl ‘a’), chlorophyll “b” (Chl ‘b’), total chlorophyll “a+b” (Total Chl) and carotenoides. The design of the experiment was conducted with leaves of common bean collected from different regions of Kosovo. The experiment was completely randomly with four repetitions. Pigments were extracted by grinding 80-100 mg freshly sampled leaves in 80% (v/v) acetone/water containing MgCO3, at room temperature, preserved in the dark for 24 hours. Concentration of chlorophyll and carotenoid content was measured by spectrophotometer using absorbance recorded at 663 nm, 644 nm and 452.3 nm for maximum absorption of Chl ‘a’, Chl ‘b’, and carotenoids respectively. According to our data the differences between populations for Chl ‘a’, and Chl ‘b’ was significantly higher at level of probability LSDp=0.01. The average values for Chl ‘a’, was 1.67 mg.g-1, while for Chl‘b’was 0.74 mg.g-1. In addition, the results for carotenoids content between populations were with high differences.

scholarly journals EVALUATION OF SALINITY STRESS EFFECTS ON CHANGES IN PHOTOSYNTHETIC PIGMENTS, HYDROGEN PEROXIDE AND OSMOLYTES IN SENSITIVE AND TOLERANT CULTIVARS OF WHEAT CROP

2020 ◽  
Vol 21 (supplement 1) ◽  
Author(s):  
Rasoul Khodavirdivand Keshtiban ◽  
Hassan Soltanloo ◽  
Seyedeh Sanaz Ramazanpour ◽  
Vahid Shariati ◽  
Vahid Shariati
Keyword(s):  
Hydrogen Peroxide ◽  
Chlorophyll A ◽  
Salinity Stress ◽  
Chinese Spring ◽  
Sampling Time ◽  
Wheat Crop ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Wheat Cultivars ◽  
Total Carbohydrates

Investigation of wheat response to salinity stress can help to better understand the effective defense mechanisms of salinity stress tolerance. For this purpose, biochemical and physiological traits related to salinity tolerance in wheat cultivars were evaluated at Gorgan University of Agricultural Sciences and Natural Resources in 2019. Experimental factors, included two wheat crop cultivars (Sarc and Chinese spring as tolerant and susceptible wheat cultivars, respectively) and sampling time series (zero or control, 24, 48, 72, and 96 h) were examined in a factorial experiment based on a completely randomized design with three replications. Salinity stress was applied with sodium chloride at a concentration of 250 mM to uniform 10-day-old seedlings at the two-leaf stage, followed by sampling of shoot tissue. The studied traits were hydrogen peroxide (H2O2 ), chlorophyll a, chlorophyll b, total chlorophyll, chlorophyllase, carotenoids, proline, and total carbohydrates. Results of analysis of variance (ANOVA) indicated significant effects of genotype, time, and interaction of genotype × time (except H2O2 and total carbohydrates) on all the studied traits. Results of interaction of genotype × time showed although the trend of changes in the studied traits, depending on the type of cultivar and the sampling time were different, but generally, the susceptible Chinese spring cultivar contained higher levels of chlorophyllase and carotenoids than the control time at the end of sampling time and also higher H2O2 levels than the Sarc tolerant cultivar, while the Sarc tolerant cultivar, on the other hand, contained higher levels of chlorophyll a, chlorophyll b, total chlorophyll, and proline than the control time at the end of sampling time and also greater total carbohydrates than the susceptible Chinese spring cultivar. The results confirm the higher capacity of the antioxidant defense system of Sarc tolerant cultivar than the susceptible Chinese spring cultivar. Therefore

scholarly journals Effect of organic and inorganic fertilizer on phytochemical constituents in sunflower

2017 ◽  
Vol 1 (4) ◽  
pp. 18
Author(s):  
S. Manikandan ◽  
P. Thamizhiniyan
Keyword(s):  
Beneficial Effect ◽  
Chlorophyll A ◽  
Organic Fertilizer ◽  
Inorganic Fertilizer ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Phytochemical Constituents ◽  
Organic And Inorganic Fertilizers ◽  
Phytochemical Constituent

The present study was conducted to characterize the effect of organic and inorganic fertilizer on the phytochemical constituents of sun flower. The phytochemical constituent such as chlorophyll a, chlorophyll b, total chlorophyll and carotenoid content were analyzed. From the result it was observed that both the treatments had significantly improved the phytochemical constituents of sunflower. Among the treatments, organic fertilizer is found to be more efficient than inorganic fertilizer over control. The present work indicates towards the beneficial effect or organic and inorganic fertilizers for the improvement of sunflower.

scholarly journals EFFECT OF LEAVES EXTRACT OF IPOMOEA CAIRICA ON CHLOROPHYLL AND CAROTENOID IN PARTHENIUM HYSTEROPHOROUS L.

2016 ◽  
Vol 4 (4) ◽  
pp. 103-107
Author(s):  
Deepa Srivastava ◽  
K. Shukla
Keyword(s):  
Chlorophyll A ◽  
Foliar Application ◽  
Carotenoid Content ◽  
Distilled Water ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Leaf Powder ◽  
Chlorophyll A And B ◽  
Noxious Weed ◽  
And Control

Photosynthetic effects of the leaves extracts from Ipomoea cairica on Partheniumhysterophorus L were studied. Ipomoea cairica dried leaf powder (20g) was dissolved in distilled water (200ml) for foliar application. The seedlings were maintained under natural condition in Insect free net house. The experiment was carried out to analyze the effects of Ipomoea cairica leaves extract by measuring chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll and carotenoid contents in Parthenium. The experimental plants were sprayed 20 days after emergence and control plants were maintained by a simultaneous spray of distilled water. Leaves of Partheniumhysterophorus were collected at 5 days interval following the foliar application of Ipomoea cairica leaves extract up to 15th day. Results showed that Chlorophyll (total chlorophyll, chlorophyll a and b) and carotenoid content was adversely affected as the duration of Ipomoea cairica leaves extract increased. This potential of Ipomoea cairica can be used as herbicide to control noxious weed Partheniumhysterophorous.

scholarly journals III. Volumetric estimation of sugar by an ammoniated cupric test giving reduction without precipitation

1879 ◽  
Vol 28 (190-195) ◽  
pp. 260-265
Keyword(s):  
Quantitative Analysis ◽  
Quantitative Determination ◽  
Indirect Method ◽  
Metallic Oxides ◽  
Volumetric Estimation ◽  
General Experience ◽  
The One

To be able to effect the quantitative determination of a body with accuracy and facility is an important matter looked at in relation to the study of its bearings. In the case of sugar there are no reliable means of precipitating and weighing it, either alone or in combination, and thus in the chemical estimation of this principle an indirect method has to be resorted to. The only property upon which dependence can be placed, for the purpose of chemical quantitative analysis, is its reducing action, under the influence of heat, upon certain metallic oxides, and that of copper is the one which general experience shows to answer best.

scholarly journals Interactive Impact of Biochar and Arbuscular Mycorrhizal on Root Morphology, Physiological Properties of Fenugreek (Trigonella foenum-graecum L.) and Soil Enzymatic Activities

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2341
Author(s):  
Dilfuza Jabborova ◽  
Kannepalli Annapurna ◽  
Ravish Choudhary ◽  
Subrata Nath Bhowmik ◽  
Said E. Desouky ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Root Length ◽  
Root Volume ◽  
Trigonella Foenum Graecum ◽  
Total Chlorophyll Content ◽  
Chlorophyll A Content

Arbuscular mycorrhizal fungi (AMF) inoculation and biochar amendment has been reported to improve the growth of several crop plant; however, their role in stress amelioration individually as well as in combination has not been worked out. Limited information is available about the synergistic use of biochar and Arbuscular Mycorrhizal Fungi (AMF). Here, we investigated the synergistic effect of biochar and AMF on plant development, root architecture, the physiological performance of fenugreek (Trigonella foenum-graecum), and soil enzymatic activities. Biochar and AMF were shown to have a considerable effect on plant height, according to the data (53.3 and 66.6%, respectively), leaf number (22.5 and 45.1%), total root length (19.8 and 40.1%), root volume (32.1 and 71.4%), chlorophyll a content (26.0 and 17.8%), chlorophyll b content (50.0 and 28.9%), total chlorophyll content (30.0 and 18.1%), and carotenoid content (60.0 and 48.0%) over the control treatment. There was a considerable increase in plant height when biochar and AMF were combined together by 80.9%, total root length by 68.9%, projected area by 48.7%, root surface area by 34.4%, root volume by 78.5%, chlorophyll a content by 34.2%, chlorophyll b content by 68.4%, total chlorophyll content by 44.5%, and carotenoid content by 84.0% compared to the control. Our results recommend that the combination of biochar and AMF is advantageous in fenugreek growth, microbial biomass, and soil enzyme activities.

Effects of Phosphorus Stress on Photosynthetic Pigments in Sagittaria sagittifolia

2014 ◽  
Vol 1073-1076 ◽  
pp. 118-121
Author(s):  
Qing Qing He ◽  
Zhou Li Liu ◽  
Wei Chen ◽  
Lin Hao ◽  
Jiao Jiao Qin ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Phosphorus Stress ◽  
Sagittaria Sagittifolia ◽  
Chlorophyll A Content

The photosynthetic pigments of Sagittaria sagittifolia, including total chlorophyll content, chlorophyll a content, chlorophyll b content and carotenoid content, were investigated after 0, 7, 14 and 21 d exposure to 3 mg L-1 P. After 7 d P-exposure, total chlorophyll content, chlorophyll a content and chlorophyll b content increased significantly compared with the control, and then decreased with time extended. Carotenoid content increased significantly after 14 d P-exposure compared with the control, and then decreased slightly after 21 d P-exposure.

Two-Stage Procedure for the Quantitative Determination of Autoprothrombin III Concentration and Some Applications

1967 ◽  
Vol 18 (01/02) ◽  
pp. 198-210 ◽  
Author(s):  
Ronald S Reno ◽  
Walter H Seegers
Keyword(s):  
Prothrombin Time ◽  
Factor Vii ◽  
Two Stage ◽  
Pronounced Increase ◽  
One Stage ◽  
Assay Procedure ◽  
Bovine Plasma ◽  
The One ◽  
Autoprothrombin C

SummaryA two-stage assay procedure was developed for the determination of the autoprothrombin C titre which can be developed from prothrombin or autoprothrombin III containing solutions. The proenzyme is activated by Russell’s viper venom and the autoprothrombin C activity that appears is measured by its ability to shorten the partial thromboplastin time of bovine plasma.Using the assay, the autoprothrombin C titre was determined in the plasma of several species, as well as the percentage of it remaining in the serum from blood clotted in glass test tubes. Much autoprothrombin III remains in human serum. With sufficient thromboplastin it was completely utilized. Plasma from selected patients with coagulation disorders was assayed and only Stuart plasma was abnormal. In so-called factor VII, IX, and P.T.A. deficiency the autoprothrombin C titre and thrombin titre that could be developed was normal. In one case (prethrombin irregularity) practically no thrombin titre developed but the amount of autoprothrombin C which generated was in the normal range.Dogs were treated with Dicumarol and the autoprothrombin C titre that could be developed from their plasmas decreased until only traces could be detected. This coincided with a lowering of the thrombin titre that could be developed and a prolongation of the one-stage prothrombin time. While the Dicumarol was acting, the dogs were given an infusion of purified bovine prothrombin and the levels of autoprothrombin C, thrombin and one-stage prothrombin time were followed for several hours. The tests became normal immediately after the infusion and then went back to preinfusion levels over a period of 24 hrs.In other dogs the effect of Dicumarol was reversed by giving vitamin K1 intravenously. The effect of the vitamin was noticed as early as 20 min after administration.In response to vitamin K the most pronounced increase was with that portion of the prothrombin molecule which yields thrombin. The proportion of that protein with respect to the precursor of autoprothrombin C increased during the first hour and then started to go down and after 3 hrs was equal to the proportion normally found in plasma.

SIMPLIFIED DEXAMETHASONE SUPPRESSION TEST

1969 ◽  
Vol 61 (2) ◽  
pp. 219-231 ◽  
Author(s):  
V. H. Asfeldt
Keyword(s):  
Cushing’S Syndrome ◽  
Normal Subjects ◽  
Cushing's Syndrome ◽  
Dexamethasone Suppression Test ◽  
Clinical Value ◽  
Suppression Test ◽  
The One ◽  
Steroid Excretion ◽  
Dexamethasone Suppression

ABSTRACT This is an investigation of the practical clinical value of the one mg dexamethasone suppression test of Nugent et al. (1963). The results, evaluated from the decrease in fluorimetrically determined plasma corticosteroids in normal subjects, as well as in cases of exogenous obesity, hirsutism and in Cushing's syndrome, confirm the findings reported in previous studies. Plasma corticosteroid reduction after one mg of dexamethasone in cases of stable diabetes was not significantly different from that observed in control subjects, but in one third of the insulin-treated diabetics only a partial response was observed, indicating a slight hypercorticism in these patients. An insufficient decrease in plasma corticosteroids was observed in certain other conditions (anorexia nervosa, pituitary adenoma, patients receiving contraceptive or anticonvulsive treatment) with no hypercorticism. The physiological significance of these findings is discussed. It is concluded that the test, together with a determination of the basal urinary 17-ketogenic steroid excretion, is suitable as the first diagnostic test in patients in whom Cushing's syndrome is suspected. In cases of insufficient suppression of plasma corticosteroids, further studies, including the suppression test of Liddle (1960), must be carried out.

A Validated 2D-LC-UV Method for Simultaneous Determination of Imatinib and N-demethylimatinib in Plasma and Its Clinical Application for Therapeutic Drug Monitoring with GIST Patients

2020 ◽  
Vol 17 ◽  
Author(s):  
Houli Li ◽  
Di Zhang ◽  
Xiaoliang Cheng ◽  
Qiaowei Zheng ◽  
Kai Cheng ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
One Dimension ◽  
Therapeutic Drug ◽  
Plasma Samples ◽  
Column Temperature ◽  
Injection Volume ◽  
Target Analytes ◽  
The One ◽  
Middle Column

Background: The trough concentration (Cmin) of Imatinib (IM) is closely related to the treatment outcomes and adverse reactions of patients with gastrointestinal stromal tumors (GIST). However, the drug plasma level has great interand intra-individual variability, and therapeutic drug monitoring (TDM) is highly recommended. Objective: To develop a novel, simple, and economical two-dimensional liquid chromatography method with ultraviolet detector (2D-LC-UV) for simultaneous determination of IM and its major active metabolite, N-demethyl imatinib (NDIM) in human plasma, and then apply the method for TDM of the drug. Method: Sample was processed by simple protein precipitation. Two target analytes were separated on the one-dimension column, captured on the middle column, and then transferred to the two-dimension column for further analysis. The detection was performed at 264 nm. The column temperature was maintained at 40˚C and the injection volume was 500 μL. Totally 32 plasma samples were obtained from patients with GIST who were receiving IM. Method: Sample was processed by simple protein precipitation. Two target analytes were separated on the one-dimension column, captured on the middle column, and then transferred to the two-dimension column for further analysis. The detection was performed at 264 nm. The column temperature was maintained at 40˚C and the injection volume was 500 μL. Totally 32 plasma samples were obtained from patients with GIST who were receiving IM. Conclusion: The novel 2D-LC-UV method is simple, stable, highly automated and independent of specialized technicians, which greatly increases the real-time capability of routine TDM for IM in hospital.

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