scholarly journals Optimizing the micropropagation protocol for Rosa canina L. elite genotype propagation in the Belgrade area

2021 ◽  
pp. 87-96
Author(s):  
Marija Markovic ◽  
Danijela Djunisijevic-Bojovic ◽  
Dragana Skocajic ◽  
Marijana Milutinovic ◽  
Katarina Buvac
Keyword(s):  
Optimal Time ◽  
Contamination Rate ◽  
Local Conditions ◽  
Iron Chelate ◽  
Rosa Canina ◽  
Planting Material ◽  
Mine Lands ◽  
Dog Rose ◽  
Number Of Shoots

Rosa canina L. (dog rose) is an important ornamental, edible and medicinal plant. It has been used as a rootstock for ornamental roses, grown in plantations for fruit harvesting and it is suitable for revegetation of abandoned mine lands. The propagation of native genotypes that are well adapted to local conditions can provide planting material for both revegetation and plantation purpose. Micropropagation is the most suitable method for a rapid vegatative propagation of selected wild genotypes, but an increased presence of pathogens as well as higher contamination rate during culture establishment were expected. An occurrence of a specific Fe-chlorosis during in vitro propagation of roses is also possible. Therefore, the optimal period and disinfection protocol for establishing sterile in vitro culture of selected genotypes of dog rose was investigated, as well as an effect of increasing the FeEDTA concentration in the MS medium during multiplication phase. The obtained results showed that the optimal time for taking initial explants corresponds to optimal time for taking green cuttings in traditional vegetative propagation by softwood cuttings, and the best results were achieved using shoots collected in the first week of May, when the flowers were open. The iron chelate concentration in the medium affected the mean number of shoots, and doubling of its concentration resulted in a considerably higher number of shoots per explant.

scholarly journals The potential of the wild dog rose (Rosa canina) to mitigate in vitro rumen methane production

2011 ◽  
Vol 20 (2) ◽  
pp. 285-299 ◽  
Author(s):  
M. Szumacher-Strabel ◽  
P. Zmora ◽  
E. Roj ◽  
A. Stochmal ◽  
E. Pers-Kamczyc ◽  
...  
Keyword(s):  
Methane Production ◽  
Rosa Canina ◽  
Wild Dog ◽  
Dog Rose

scholarly journals RESPON EKSPLAN PISANG KLUTUK (Musa paradisiaca L.) TERHADAP KONSENTRASI EKSTRAK BIJI PINANG MUDA DAN AIR KELAPA MUDA SECARA IN VITRO

2021 ◽  
Vol 35 (3) ◽  
pp. 135-142
Author(s):  
Zulkifli ◽  
Selvia Sutriana
Keyword(s):  
Tissue Culture ◽  
Culture Technique ◽  
Coconut Water ◽  
Areca Nut ◽  
Planting Material ◽  
Randomized Design ◽  
Significant Difference ◽  
Single Response ◽  
Number Of Shoots

Nurseries play an important role in banana development. The tissue culture technique is an alternative to produce quality seeds in large quantities, uniform and produced in a short time and free of pathogens. Areca nut extract is an organic material that contains tannin and can replace bicycling used as a disinfectant in tissue culture activities. Coconut water is a food reserve that contains vitamins and growth substances, so it can stimulate germination. The purpose of the study was to examine the interaction and single response of klutuk banana explants to the concentration of areca nut extract and young coconut water in vitro. This research was carried out at the Biotechnology Laboratory, Faculty of Agriculture, the Islamic University of Riau with the planting material used Pisang Klutuk weevil with the method used in a factorial Completely Randomized Design (CRD) with 4 x 4.  The first factor was seed extract Young areca nut and the second factor was Young Coconut Water. The parameters observed were the percentage of live explants, the percentage of contaminated explants, the age of shoot emergence, and the number of shoots. The observation results were analyzed statistically and continued with the further test of Honest Significant Difference (HSD) at the 5% level. It was found that the best treatment was P2K2 (20% young betel nut extract (200 ml + 800 ml distilled water) and young coconut water 20 ml/liter media), where the percentage of life was 100%, the percentage of contamination was 0%, the age of shoots appeared was 37.67 days, and the number of shoots was 5 strands.

IN VITRO MICROPROPAGATION OF DOG ROSE (ROSA CANINA L.)

2012 ◽  
pp. 911-913 ◽  
Author(s):  
M. Shirdel ◽  
A. Motallebi-Azar ◽  
N. Mahna
Keyword(s):  
Rosa Canina ◽  
In Vitro Micropropagation ◽  
Dog Rose

scholarly journals EFFECT OF PHYTOHORMONES AND THEIR DIVERSE CONCENTRATIONS ON REGENERATION OF ROSE (ROSA HYBRIDA L.)

2020 ◽  
Vol 23 (1) ◽  
pp. 46-51
Author(s):  
Memon Amjad Ali ◽  
Ghulam Mangrio
Keyword(s):  
Basal Medium ◽  
Rosa Hybrida ◽  
Shoot Length ◽  
Root Induction ◽  
Root Initiation ◽  
Planting Material ◽  
Number Of Leaves ◽  
Analysis Of Variation ◽  
Number Of Shoots

Roses most important regularly used for ornamental, medicinal and aromatic rationale in the world. The relevance of plant tissue culture technology to produce planting material of rose in masses depends on the availability of an effective regeneration protocol. The present experiment was done to scrutinize for appropriate basal medium of Murashige and Skoog (1962), phytohormones with their diverse concentrations influence for establish In vitro shoot and root induction of rose (Rosa hybrida L.). The statically analysis of variation explain that least days to initiation, number of shoots, length of shoot cm, number of leaves, days taken in root initiation and number of roots were significant @ 5% possibility. Increase evidence viewing that experimental conclusion exhibit that minimum days to initiation, utmost number of shoots bottle-1, shoot length bottle-1 and number of leaves bottle-1 be record within the concentration of MS + NAA 0.5 mgL-1 + BAP 2 mgL-1. Hence forward minimum days taken in root initiation, highest roots number recorded at 1/2MS + NAA 1.0mg/l + IBA 1.0 mg/l respectively. In vitro healthy and complete plantlets successfully were shifted in to different pot mixtures, supreme survival % recorded at Soil+sand+FYM (1:1:1).

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P< 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P< 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P< 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P< 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

Efficient reactivation of Xenopus erythrocyte nuclei in Xenopus egg extracts

1995 ◽  
Vol 108 (6) ◽  
pp. 2187-2196 ◽  
Author(s):  
L.J. Wangh ◽  
D. DeGrace ◽  
J.A. Sanchez ◽  
A. Gold ◽  
Y. Yeghiazarians ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Somatic Cell ◽  
Nuclear Transplantation ◽  
Optimal Time ◽  
Genome Replication ◽  
Cell Nuclei ◽  
Egg Extracts ◽  
Xenopus Egg ◽  
Xenopus Egg Extracts

Rapid genome replication is one of the hallmarks of the frog embryonic cell cycle. We report here that complete reactivation of quiescent somatic cell nuclei in Xenopus egg extracts depends on prior restructuring of the nuclear substrate and prior preparation of cytoplasmic extract with the highest capacity to initiate and sustain DNA synthesis. Nuclei from mature erythrocytes swell, replicate their DNA efficiently, and enter mitosis in frozen/thawed extracts prepared from activated Xenopus eggs, provided the nuclei are first treated with trypsin, heparin, and an extract prepared from unactivated, meiotically arrested, eggs. Optimal replicating extracts are prepared from large batches of unfertilized eggs that are synchronously activated into the cell cycle for 28 minutes (at 20 degrees C). Because the Xenopus cell cycle progresses so rapidly, extracts prepared just a few minutes before or after this time have substantially lower DNA synthetic capacities. At the optimal time and temperature, eggs have just reached the G1/S boundary of the first cell cycle. This fact was revealed by injecting and replicating an SV40 plasmid in intact unfertilized eggs as described previously. We estimate that under optimal conditions approximately 6.14 × 10(9) base pairs of DNA/per nucleus are synthesized in 30–40 minutes, a rate that rivals that observed in the zygotic nucleus. The findings reported here are one step in our long term effort to develop a new in vitro/in vivo approach to nuclear transplantation. Nuclear transplantation in amphibian embryos has been used to establish that the genomes of many types of differentiated somatic cells are pluripotent. But very few such nuclei have ever developed into advanced tadpoles or adult frogs, probably because somatic nuclei injected directly into activated eggs fail to reactivate quickly enough to avoid being damaged during first mitosis. We have already shown that unfertilized eggs can be injected prior to activation of the first cell cycle. Future experiments will reveal whether in vitro reactivated somatic cell nuclei transplanted into such eggs reliably reach advanced stages of development.

scholarly journals Biofertlizer Lumbrical improves the growth and ex vitro acclimatization of micropropagated pear plants

2021 ◽  
Vol 22 (1) ◽  
pp. 17-30
Author(s):  
Nataliya Dimitrova ◽  
Lilyana Nacheva ◽  
Małgorzata Berova ◽  
Danuta Kulpa
Keyword(s):  
Woody Species ◽  
Photosynthetic Photon Flux Density ◽  
Stem Length ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Ex Vitro ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Number Of Leaves

In vitro micropropagation of plants is highly useful for obtaining large quantities of planting material with valuable economic qualities. However, plantlets grow in vitro in a specific environment and the adaptation after the transfer to ex vitro conditions is difficult. Therefore, the acclimatization is a key step, which mostly determines the success of micropropagation. The aim of this investigation was to study the effect of the biofertlizer Lumbrical on ex vitro acclimatization of micropropagated pear rootstock OHF 333 (Pyrus communis L.). Micropropagated and rooted plantlets were potted in peat and perlite (2:1) mixture with or without Lumbrical. They were grown in a growth chamber at a temperature of 22&plusmn;2 &deg;C and photoperiod of 16/8 hours supplied by cool-white fluorescent lamps (150 &micro;mol m-2 s-1 Photosynthetic Photon Flux Density, PPFD). The plants were covered with transparent foil to maintain the high humidity, and ten days later, the humidity was gradually decreased. Biometric parameters, anatomic-morphological analyses, net photosynthetic rate and chlorophyll a fluorescence (JIP test) were measured 21 days after transplanting the plants to ex vitro conditions. The obtained results showed that the plants, acclimatized ex vitro in the substrate with Lumbrical, presented better growth (stem length, number of leaves, leaf area and fresh mass) and photosynthetic characteristics as compared to the control plants. This biostimulator could also be used to improve acclimatization in other woody species

scholarly journals In vitro propagation of six selected sugarcane mutant clones through leaf explants

2021 ◽  
Vol 883 (1) ◽  
pp. 012075
Author(s):  
R Purnamaningsih ◽  
D Sukmadjaja ◽  
S Suhesti ◽  
S Rahayu
Keyword(s):  
Callus Induction ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Medium Composition ◽  
Culture Techniques ◽  
High Productivity ◽  
Media Formulation ◽  
Number Of Shoots

Abstract Six mutant clones of sugarcane with high productivity have been produced through tissue culture techniques combined with mutations using gamma-ray irradiation and Ethyl Methane Sulfonate. The six mutant clones have been tested for stability in the field. They are proven to have high productivity and yields, so that they are very potential to be developed as superior varieties. To support the planting material sufficiency of these clones, an efficient propagation method was needed. Media formulations with different physical properties and composition of growth regulators were tested to obtain high seedling propagation rates. The media formulation for callus induction was Murashige dan Skoog (MS) + 3 mg/l 2,4-D + 3 g/l casein hydrolysate + 3% sucrose and for shoot regeneration was MS + 0,5 mg/l BA + 0,1 mg/l IBA + 100 mg/l PVP and 2% sucrose. Shoot proliferation was carried out on MS liquid (1, ½) + (0.3; 0.5 mg/l) BA + 0.1 mg/l IBA + 1 mg/l Kinetin + (0; 0.5 mg/l) GA3+ sucrose 2%. The results showed that callus induction, callus regeneration, and shoot proliferation of sugarcane mutant clones were influenced by the genotype and medium composition. The fastest callus induction was obtained from the MSP-4 clone (5.82 days), and the longest was MSB-7 (8.82 days). The largest callus diameter was obtained from MSB-6 clone on MS medium containing 1 mg/l BA, 100 mg/l PVP, and 2% sucrose. The highest number of shoots was obtained from the MSB-6 clone, while the least number of shoots conducted from the MSB-8 clone. The MSB-8 clones were more difficult to regenerate compared to the others. The best media formulation for shoot proliferation was ½ MS containing 0.5 mg/l BA, 1 mg/l Kinetin, and 0.1 mg/l IBA, while the best formulation for rooting was ½ MS.

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