scholarly journals The influence of fullerenol on antioxidative enzyme activity in irradiated human erythroleukemic cell line (K562)

2007 ◽  
Vol 61 (3) ◽  
pp. 164-166
Author(s):  
Visnja Bogdanovic ◽  
Karmen Stankov ◽  
Aleksandra Nikolic ◽  
Ivana Icevic ◽  
Slavica Solajic ◽  
...  
Keyword(s):  
Cell Culture ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
X Rays ◽  
Control Groups ◽  
Sod Activity ◽  
Applied Dose ◽  
Erythroleukemic Cell ◽  
Erythroleukemic Cell Line ◽  
Total Superoxide Dismutase

Cell culture K562 samples were treated with fullerenol (C6o(OH)24) at a concentration of 10 nmol/mL and thereafter irradiated with X-rays (24Gy). The activity of gamma-glutamyltransfrease (?-GT), total superoxide-dismutase (SOD) and glutathion-peroxidase (GSH-Px) was determined 1, 24 and 48 hours after irradiation. Irradiation induces an increase in the activity of all the investigated enzymes. Fullerenol in the applied dose decreased the ?-GT activity 24 and 48 h after irradiation. The total SOD activity is increased in both pretreated groups except in the iradiated group at the 48th hour. Treatment with fullerenol before irradiation increased GSH-Px activity in irradiated groups and decreased it in the control groups.

10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Kiptiyah Kiptiyah ◽  
Widodo Widodo ◽  
Gatot Ciptadi ◽  
Aulanni’am Aulanni’Am ◽  
Mohammad A. Widodo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Culture ◽  
Superoxide Dismutase ◽  
In Silico ◽  
Cumulus Cell ◽  
Cumulus Cells ◽  
Sod Activity ◽  
In Silico Studies ◽  
Incubation Periods

AbstractBackgroundWe investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells.MethodsFor the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20 µM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentrations, with incubation periods of 24, 48, 72, and 96 h. The obtained results were confirmed by in-silico studies.ResultsThe in-vitro data revealed that SOD activity and MDA concentration increased with increasing incubation periods: SOD activity at 0 µM (1.39 ± 0.24i), 12 µM (16.42 ± 0.35ab), 16 µM (17.28 ± 0.55ab), 20 µM (17.81 ± 0.12a), with a contribution of 71.1%. MDA concentration at 0 µM (17.82 ± 1.39 l), 12 µM (72.99 ± 0.31c), 16 µM (79.77 ± 4.19b), 20 µM (85.07 ± 2.57a), with a contribution of 73.1%. Based on this, the in-silico data uncovered that 10˗gingerol induces oxidative stress in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.Conclusions10-gingerol induces oxidative stress in cumulus cells through enhancing SOD activity and MDA concentration by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.

scholarly journals THE CORRELATION BETWEEN FAT INTAKE AND PLASMA SUPEROXIDE DISMUTASE ACTIVITY WITH TELOMERE LENGTH OF MINANGKABAU ETHNICITY MEN, IN WEST SUMATERA, INDONESIA

2019 ◽  
pp. 305-309
Author(s):  
AFRIWARDI AFRIWARDI ◽  
DELMI SULASTRI ◽  
YUNIAR LESTARI ◽  
DESMAWATI DESMAWATI ◽  
PRIMA MINERVA
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Fatty Acid ◽  
Telomere Length ◽  
Saturated Fatty Acids ◽  
Cross Sectional Study ◽  
Fat Intake ◽  
Sod Activity ◽  
Cross Sectional ◽  
Total Fat

Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia. Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity. Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p>0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037). Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.

scholarly journals The influence of fullerenol on the cell number, cell area and colony forming unit ability in irradiated human erythroleukemic cell line

2007 ◽  
Vol 61 (3) ◽  
pp. 167-170 ◽  
Author(s):  
Ivana Icevic ◽  
Visnja Bogdanovic ◽  
Dragan Zikic ◽  
Slavica Solajic ◽  
Gordana Bogdanovic ◽  
...  
Keyword(s):  
Colony Formation ◽  
K562 Cells ◽  
Cell Number ◽  
Cell Area ◽  
X Rays ◽  
Colony Forming Unit ◽  
Erythroleukemic Cell ◽  
Analysis Of The Images ◽  
Erythroleukemic Cell Line ◽  
Cell Colony

DET (dye exclusion test) cell count and cell area by computer analysis of the images were determined in cell lines of human eritroleukemia (K562), which were irradiated with X-rays in one dose of 24 Gy and pretreated with 10 nmol/mL fullerenol (Cgo(OH)24). Cell samples obtained using a citocentrifuge and May-Gr?nvald Giemsi (MGG) during, were analyzed. The cell colony formation ability was monitored using quantative CFU (colony forming unit) test. Irradiation decreases the number of K562 cells, but fullerenol significantly increases cell number on 24th and 48th hour of the experiment. Cell area is larger, and the number of formed cell colonies after irradiation is significantly smaller compared to pretreated groups during the whole experiment. Pretreatment with fullerenol maintains a smaller cell area, and the number of colony formed units was larger compared to the irradiated cells.

Copper (II) Accumulation And Superoxide Dismutase Activity During Growth Of Aspergillus Niger B-77

2002 ◽  
Vol 57 (3-4) ◽  
pp. 319-322 ◽  
Author(s):  
Kolishka Tsekova ◽  
Dessislava Todorova
Keyword(s):  
Heavy Metal ◽  
Cell Culture ◽  
Superoxide Dismutase ◽  
Aspergillus Niger ◽  
Heavy Metal Ions ◽  
Microbial Growth ◽  
Biological Activities ◽  
Sod Activity ◽  
Growing Cell ◽  
A Cell

The influence of copper (II) ions on the growth, accumulation properties and superoxide dismutase (SOD) activity of a growing culture of Aspergillus niger B-77 were studied. Microbial growth, the level of copper (II) accumulation and SOD activity depended on the initial copper (II) concentration. Aspergillus niger is able to accumulate large amounts of copper (II) from the nutrient medium with 200 mg.l-1 copper (II) ions without loosing its biological activities. Addition of copper (II) ions increased the SOD activity in the growing cell cultures. The changes in enzyme activity induced by heavy metal ions might be used as an indicator of intracellular oxy-intermediate generation in a cell culture growing under stress conditions

Effects of Nonylphenol on the Enzyme Activity of SOD in Shrimp (Neocaridina heteropoda)

2014 ◽  
Vol 1073-1076 ◽  
pp. 256-258
Author(s):  
Hong Wei Wang ◽  
Qing Chao Xing ◽  
Jie Zhang ◽  
Rui Yin ◽  
Duan Bo Cai ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Sod Activity ◽  
Physiological Indicators ◽  
Relationship Of ◽  
The Relationship

The effects of different concentrations of nonylphenol on Neocaridina heteropoda were studied through testing the Superoxide Dismutase (SOD) activity in the muscle of N. heteropoda under the nonylphenol stress, then explored the relationship of the time that was N. heteropoda exposed nonylphenol and the in vivo physiological indicators. Results showed the following conclusion: in a certain range, higher concentrations of nonylphenol had heavier influence on SOD activity of shrimps; shrimps were infected significantly when they were exposed to the same concentration of nonylphenol in 2 days; the influence was reduced to the shrimp with the prolonging of time.

scholarly journals   Antioxidative mechanisms on selenium accumulation in Pteris vittata L., a potential selenium phytoremediation plant

2012 ◽  
Vol 58 (No. 3) ◽  
pp. 105-110 ◽  
Author(s):  
R.W. Feng ◽  
C.Y. Wei
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Pteris Vittata ◽  
Sod Activity ◽  
Antioxidant Responses ◽  
Industrial Activities ◽  
Chinese Brake Fern ◽  
Selenium Accumulation ◽  
Pteris Vittata L

Selenium (Se) contamination due to industrial activities has received increasing concerns. Phytoremediation has been suggested to be an efficient and feasible way to remove Se from Se-contaminated environment. Recently, an arsenic (As) hyperaccumulator Pteris vittata L. (Chinese Brake fern) was found to be a Se accumulator. This study was carried out to investigate Se accumulation mechanisms concentrating on antioxidant responses of this plant to six levels of selenite (0, 1, 2, 5, 10, and 20 mg/L). The results showed that Chinese Brake fern can accumulate a large amount of Se without any visible toxic symptoms and significant decreases in its biomass. However, the root took up more Se than the fronds. The highest concentration of Se in the roots and fronds was 1.536 mg/kg and 242 mg/kg, respectively, demonstrating a typical accumulation character to Se. Addition of 2 mg/L Se decreased, but ≥ 5 mg/L Se enhanced the production of malondialdehyde (MDA), suggesting an antioxidant role of low dosages of Se. The enzymes of catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD) contributed their anti-oxidative functions only under low dosages of Se, as shown by their increased activities at Se levels ≤ 5 mg/L and lowered activities at Se levels > 5 mg/L. The concentration of glutathione (GSH) and enzyme activity of glutathione reductase (GR) were stimulated by ≥ 5 mg/L Se. Superoxide dismutase (SOD) activity was also enhanced by 20 mg/L Se. Our results suggest that SOD, GSH and GR were likely responsible for, but enzymes of POD, APX, and CAT have limited roles in Se accumulation in Chinese Brake fern.  

scholarly journals THE CORRELATION BETWEEN FAT INTAKE AND PLASMA SUPEROXIDE DISMUTASE ACTIVITY WITH TELOMERE LENGTH OF MINANGKABAU ETHNICITY MEN, IN WEST SUMATERA, INDONESIA

2019 ◽  
Author(s):  
Afriwadi ◽  
Delmi Sulastri ◽  
Yuniar Lestari ◽  
Prima Minerva
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Fatty Acid ◽  
Telomere Length ◽  
Saturated Fatty Acids ◽  
Cross Sectional Study ◽  
Fat Intake ◽  
Sod Activity ◽  
Cross Sectional ◽  
Total Fat

Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia.Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity.Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p>0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037).Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.

Manganese superoxide dismutase in Meloidogyne incognita isolates selected for virulence on Mi-1-carrying tomato: gene expression and enzyme activity

Nematology ◽  
2014 ◽  
Vol 16 (3) ◽  
pp. 249-257
Author(s):  
Sergio Molinari ◽  
Sergio Molinari ◽  
Laura Rosso
Keyword(s):  
Gene Expression ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Meloidogyne Incognita ◽  
Manganese Superoxide Dismutase ◽  
Sod Activity ◽  
Root Knot Nematodes ◽  
Adult Females ◽  
Manganese Superoxide ◽  
Wide Range

Root knot nematodes (Meloidogyne spp.) are important pests of a wide range of crops, including tomato. Resistance of tomato to root-knot nematodes is conferred by the single dominant gene Mi-1, which currently is present in all commercially available resistant tomato cultivars. However, several resistance-breaking populations are being collected worldwide. Two isolates coming from the same standard population of Meloidogyne incognita, one selected for virulence against Mi-1 (SM1) and the other left avirulent (avr1), have been used in this study. qRT-PCR was used to detect transcript levels of the manganese superoxide dismutase (Mn-SOD) gene from (a)virulent pre-parasitic second-stage juveniles (J2). Over-expression of the Mn-SOD gene was found in the virulent isolate compared with the avirulent counterpart. The enzyme activity of membrane-bound mitochondrial Mn-SOD was assayed in J2 and adult females as the fraction of total SOD activity insensitive to hydrogen peroxide (H2O2). J2 from SM1 showed about a two-fold higher enzyme activity than J2 from avr1; conversely, no difference was found when adult females were tested. Proteins of J2 extracts were separated by n-PAGE on special mini-gels and stained for SOD. One slow migrating and three fast migrating bands were stained. SOD activity of the slow migrating band was H2O2-insensitive and enriched by treatment with the detergent Triton X-100. J2 survival was monitored in suspensions provided or not provided with the cell oxygen radical generator paraquat, at high concentration. Virulent J2 responded to paraquat treatment by increasing life extension with respect to control conditions; by contrast, avirulent J2 suffered major mortality in the presence of paraquat. Mn-SOD gene expression is discussed in relation to nematode fitness in oxidative stresses and vir phenotype.

scholarly journals Age-related changes of superoxide dismutase activity in patients with schizophrenia

2017 ◽  
Vol 74 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Vladimir Djordjevic ◽  
Dusan Lazarevic ◽  
Vladan Cosic ◽  
Marinela Knezevic ◽  
Vidosava Djordjevic
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
First Generation ◽  
Age Of Onset ◽  
First Episode ◽  
Psychotic Episode ◽  
Sod Activity ◽  
Age Related ◽  
Significant Difference ◽  
Schizophrenic Patients

Background/Aim: Superoxide dismutase (SOD) is the critical enzyme in the detoxification of superoxide radicals because those are the first species produced in the majority of biological free radical producing reactions. Inconsistent data are present about SOD activity in patients with schizophrenia. Numerous studies have shown that SOD has been elevated in chronic schizophrenic patients. However, decreased SOD activity was found in neuroleptic na?ve, first episode schizophrenic patients, in chronic-medicated patients and in chronic-unmedicated patients. The aim of this study was to examine which of the following factors including age, gender, the onset of the disease, the duration, the number of episodes, heredity, psychopathologic symptoms and drug treatment could affect erythrocyte SOD activity in patients with schizophrenia. Methods: This study included 68 consecutive patients with schizophrenia (29 males and 39 females) ranging in age from 18 to 61 years, divided into two age groups (<34 years and >34 years). SOD activity was measured in erythrocyte hemolyzates by Ransod commercially available test. Results: In the group of patients younger than 34 years SOD levels were significantly higher (1381?273 U/gHb, p=0.038) compared to the levels of the older group (1231?206 U/gHb). Gender and heredity did not induce any significant difference in SOD activity between younger and older subgroups. A significant difference in enzyme activity was found between the younger and older subgroups having the onset of the disease after 24 years of age (1408?217 U/gHb vs. 1252?213 U/gHb, p=0.031). The patients of the younger group who had more than one psychotic episode had significantly higher SOD activity (1492?298 U/gHb; p=0.009) than those who had only one episode (1256?177 U/gHb), as well as than the older subgroup with more than one episode (1253?231 U/gHb; p=0.014). Although the duration of the disease did not induce any significant difference in enzyme activity between younger and older subgroups, a significant negative correlation was obtained between SOD activity and the duration of the disease (r=-0.511, p<0.01). No significant differences were found in SOD activity between the subgroups with different PANSS scores. First generation antipsychotics were associated with elevated enzyme activity in both groups. Simultaneous treatment of patients with first generation antipsychotics and second generation antipsychotics induced a significant decrease in SOD activity in the younger group. Conclusion: Our results show that erythrocyte SOD activity is increased in the early phase of schizophrenia and that depends on age of onset of the disease, the number of psychotic episodes, the duration of the disease and medical treatment.

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