scholarly journals THE CORRELATION BETWEEN FAT INTAKE AND PLASMA SUPEROXIDE DISMUTASE ACTIVITY WITH TELOMERE LENGTH OF MINANGKABAU ETHNICITY MEN, IN WEST SUMATERA, INDONESIA

2019 ◽  
Author(s):  
Afriwadi ◽  
Delmi Sulastri ◽  
Yuniar Lestari ◽  
Prima Minerva

Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia.Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity.Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p>0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037).Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.

Author(s):  
AFRIWARDI AFRIWARDI ◽  
DELMI SULASTRI ◽  
YUNIAR LESTARI ◽  
DESMAWATI DESMAWATI ◽  
PRIMA MINERVA

Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia. Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity. Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p>0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037). Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.


Author(s):  
Prima Minerva ◽  
Delmi Sulastri ◽  
Yuniar Lestari

Background: Non-communicable diseases (NCD) are the leading cause of death globally today. In West Sumatra there has been an increase in NCD which exceeds the national average percentage. Telomere shortening is associated with occurrence of NCD. Shortening of telomeres is influenced by diet. The purpose of this study was to examine the relationship between Saturated Fatty Acid (SAFA) intake and Superoxide Dismutase (SOD) activity with the telomere length of Minangkabau ethnic men.Methods: This study was a cross sectional study. The sample of this study were 69 civil servants of Minangkabau male at district office in the Padang City, aged 40-50 years and according to the inclusion and exclusion criteria. Data on SAFA intake was obtained using Semi Quantitative Food Frequency Questionnaire (SQFFQ), SOD activity was measured using the Calorimetric method and telomere length was measured using Real Time Polymerase Chain Reaction (RT-PCR) by O’Challaghan and Fennech method. Data analysis was performed using Pearson correlation test with significance level p <0.05.Results: The results showed an average SAFA intake 10.47+3.46% calories, SOD activity 5.93+0.81units/ml and telomere length 468.94+199.25bp. Correlation test between telomere length with SAFA intake showed (p=0.338), and the correlation test between SOD activity and telomere length showed (p=0.01).Conclusions: From this study it was concluded that there was no correlation between SAFA intake and telomere length and there was a significant positive correlation between SOD activity and the telomere length of Minangkabau ethnic men.


2019 ◽  
Author(s):  
Prima Minerva ◽  
Delmi Sulastri ◽  
Yuniar Lestari

Background: Non-communicable diseases (NCD) are the leading cause of death globally today. In West Sumatra, there has been an increase in NCD which exceeds the national average percentage. Telomere shortening is associated with occurrence of NCD. Shortening of telomeres is influenced by diet. The purpose of this study was to examine the relationship between Saturated Fatty Acid (SAFA) intake and Superoxide Dismutase (SOD) activity with the telomere length of Minangkabau ethnic men.Methods: This study was a cross sectional study. The sample of this study were 69 civil servants of Minangkabau male at district office in the Padang City, aged 40-50 years and according to the inclusion and exclusion criteria. Data on SAFA intake was obtained using Semi Quantitative Food Frequency Questionnaire (SQFFQ), SOD activity was measured using the Calorimetric method and telomere length was measured using Real Time Polymerase Chain Reaction (RT-PCR) by O’Challaghan and Fennech method. Data analysis was performed using Pearson correlation test with significance level p &lt;0.05.Results: The results showed an average SAFA intake 10.47+3.46% calories, SOD activity 5.93+0.81units/ml and telomere length 468.94+199.25bp. Correlation test between telomere length with SAFA intake showed (p=0.338), and the correlation test between SOD activity and telomere length showed (p=0.01).Conclusions: From this study it was concluded that there was no correlation between SAFA intake and telomere length and there was a significant positive correlation between SOD activity and the telomere length of Minangkabau ethnic men.


2015 ◽  
Vol 113 (7) ◽  
pp. 1086-1095 ◽  
Author(s):  
Riitta Freese ◽  
Liisa Korkalo ◽  
Bengt Vessby ◽  
Siv Tengblad ◽  
Elina M. Vaara ◽  
...  

Many African diets are low in fat but are currently changing because of nutrition transition. We studied fat and fatty acid (FA) intake and the essential fatty acid (EFA) status of adolescent girls (aged 14–19 years, n 262) in Zambezia Province, central Mozambique. A cross-sectional study was carried out in a city as well as in the towns and rural villages of a coastal and an inland district. Dietary intake and FA sources were studied in a 24 h dietary recall. FA compositions of cholesteryl esters and phospholipids of non-fasting serum samples were analysed by GLC. Fat intake was low (13–18 % of energy) in all areas. Coconut and palm oil were the main sources of fat, and soyabean oil and maize were the main sources of PUFA. Compared to Food and Agriculture Organization/WHO 2010 recommendations, intake of linoleic acid (LA, 18 : 2n-6) was inadequate in the coastal district, and intakes of n-3 PUFA were inadequate in all areas. FA compositions of serum lipids differed between areas. The proportions of LA tended to be highest in the city and lowest in the rural areas. The phospholipid mead (20 : 3n-9):arachidonic acid (20 : 4n-6) ratio did not indicate EFA insufficiency. LA proportions in phospholipids were low, but those of long-chain n-6 and n-3 PUFA were high in comparison with Western adolescents. To conclude, fat sources, FA intake and EFA status differed between adolescent girls living in different types of communities. Fat intake was low, but EFA insufficiency was not indicated.


PEDIATRICS ◽  
1990 ◽  
Vol 86 (4) ◽  
pp. 520-526 ◽  
Author(s):  
R. Sue McPherson ◽  
Milton Z. Nichaman ◽  
Harold W. Kohl ◽  
Debra B. Reed ◽  
Darwin R. Labarthe

To investigate the nutrient intake and food use patterns among schoolchildren, diet was assessed among 138 children and adolescents in grades 5 through 12 using three random, nonconsecutive, 1-day food records. Mean intake of total fat, saturated fat, and polyunsaturated fat as percent of calories was 35.6%, 13.4%, and 6.6%, respectively. Among all subjects, 17% consumed diets containing &lt;30% of calories from fat, 34% consumed3≥8% of calories from fat, 7% consumed &lt;10% of calories from saturated fatty acids, and &gt;97% ate &lt;300 mg of cholesterol per day. While intake of calories, sodium, and β-carotene per 1000 kcal was higher in subjects consuming higher fat diets, intake of other micronutrients was either higher among those eating low-fat diets or did not differ by level of fat intake. Differences were seen in the amount of saturated fat and cholesterol that individual food sources contributed to the diets of subjects eating high and low fat diets. These cross-sectional data show that a substantial proportion of children and adolescents in this population are consuming diets low in fat and cholesterol without systematic differences in intake of other nutrients, suggesting that current dietary guidelines regarding fat intake are attainable within the current food use pattern of healthy, school-aged children and adolescents.


2001 ◽  
Vol 4 (2) ◽  
pp. 227-232 ◽  
Author(s):  
Stephanie Harper ◽  
Ingrid HE Rutishauser

AbstractObjectivesTo assess the fat intake and knowledge about the fat content of foods consumed by a sample of self reported users of reduced-fat dairy products.DesignCross-sectional study of a population-based sample of women shoppers.SettingA small, rural town (population approximately 6000) in central Victoria, Australia.SubjectsSeventy-eight women aged 25–50 years, who regularly used at least one reduced-fat dairy product.ResultsMean reported intake of total fat was lower while intake of dairy fat was similar to that of a national sample of women of the same age both in the whole sample and when under-reporters were excluded. The ability to identify major sources of fat in the diet as reported appeared to be limited. Less than half of the subjects were able to correctly estimate the fat content of reduced-fat dairy products relative to regular products and about one quarter of subjects reported replacing one kind of oil or fat with another as a strategy to reduce fat intake. Subjects were generally aware of the need to ‘eat less fat’ but few could articulate specific recommendations. A number of subjects reported using low fat diets to control their weight but few subjects appeared to understand the connection between fat intake and energy intake.ConclusionsThe findings of this study raise important questions about how nutrition advice is understood and implemented by consumers, particularly the message to reduce fat intake and the role of energy balance in weight management. They also highlight the difficulty of interpreting information on food intake, in subjects who have modified their diet by reducing intake of specific foods.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yasaman Aali ◽  
Farideh Shiraseb ◽  
Faezeh Abaj ◽  
Fariba koohdani ◽  
Khadijeh Mirzaei

Abstract Background It has been reported that dietary fats and genetic factors in individuals are associated with the pattern of fat distribution. This study aimed to evaluate the interaction between dietary fats intake and Caveolin1 (CAV-1) rs 3807s992 polymorphism with fat distribution in overweight and obese women. Methods A total of 221 participants were included in the current cross-sectional study. Body composition, biochemical parameters were evaluated by body composition analyzer and Pars Azmoon kits and genotypes determination was performed by PCR–RFLP, dietary fats were measured using a validated semi-quantitative food frequency questionnaire (FAQ). Results The frequency of GG, AA and AG genotypes were 53.1, 24.6, and 22.3%, respectively, and the mean intake of total dietary fat intake was 97.47 ± 36.87 g. There was positive significant interaction between total fat intake and AA genotype on visceral fat level (p = 0.001), trunk fat (p = 0.01) and waist circumference (p = 0.05), positive significant interaction between total fat intake and AG genotype on the waist to hip ratio (WHR) (p = 0.02) and visceral fat level (p = 0.05), positive borderline significant interaction between saturated fatty acid and AA genotype on the trunk fat (p = 0.06), and between trans-fatty acids and AG genotype on WHR (p = 0.04), visceral fat level (p = 0.01), and between monounsaturated fatty acid and AG genotype on WHR (p = 0.04), and a borderline interaction between polyunsaturated fatty acid and AA genotypes on visceral fat level (p = 0.06), negative significant interaction between AG genotypes and linolenic acid on WHR (p = 0.04), borderline significant interaction between ALA and AG genotype on WHR (p = 0.06). Conclusions Our findings showed that CAV-1 rs 3807992 polymorphism and dietary fats were associated with fat distributions in individuals.


Nutrients ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1371 ◽  
Author(s):  
Sahar Yammine ◽  
Farah Naja ◽  
Hani Tamim ◽  
Mona Nasrallah ◽  
Carine Biessy ◽  
...  

There have been increases in the incidence of obesity in Lebanon over the past few decades. Fatty acid intake and metabolism have been postulated to influence obesity, but few epidemiological studies have been conducted. The aim of this study was to investigate the correlation between serum fatty acid levels and indicators of obesity in a cross-sectional study nested within a cohort of 501 Lebanese adults residing in Greater Beirut. A total of 395 available serum samples (129 men, 266 women) were profiled for phospholipid fatty acid composition. Spearman correlation coefficients adjusted for relevant confounders and corrected for multiple testing were calculated between serum fatty acids, desaturation indices, and indicators of adiposity (body mass index (BMI) and waist). BMI was significantly positively correlated with saturated fatty acids in men (r = 0.40, p < 0.0001, q < 0.0001) and women (r = 0.33, p < 0.0001, q < 0.0001). BMI was significantly positively correlated with monounsaturated fatty acid palmitoleic acid in women (r = 0.15, p = 0.01, q = 0.03). This study suggests that high blood levels of some saturated fatty acids and the monounsaturated fatty acid palmitoleic acid, likely derived from both dietary intakes of saturated fatty acids and endogenous lipogenesis, may have been associated with adiposity in the Lebanese population. The causality of these associations needs to be explored in experimental settings.


Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 2032
Author(s):  
Judit Companys ◽  
Maria José Gosalbes ◽  
Laura Pla-Pagà ◽  
Lorena Calderón-Pérez ◽  
Elisabet Llauradó ◽  
...  

We aimed to differentiate gut microbiota composition of overweight/obese and lean subjects and to determine its association with clinical variables and dietary intake. A cross-sectional study was performed with 96 overweight/obese subjects and 32 lean subjects. Anthropometric parameters were positively associated with Collinsella aerofaciens, Dorea formicigenerans and Dorea longicatena, which had higher abundance the overweight/obese subjects. Moreover, different genera of Lachnospiraceae were negatively associated with body fat, LDL and total cholesterol. Saturated fatty acids (SFAs) were negatively associated with the genus Intestinimonas, a biomarker of the overweight/obese group, whereas SFAs were positively associated with Roseburia, a biomarker for the lean group. In conclusion, Dorea formicigenerans, Dorea longicatena and Collinsella aerofaciens could be considered obesity biomarkers, Lachnospiraceae is associated with lipid cardiovascular risk factors. SFAs exhibited opposite association profiles with butyrate-producing bacteria depending on the BMI. Thus, the relationship between diet and microbiota opens new tools for the management of obesity.


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