scholarly journals Determination of specific antibodies titre to salmonella enteritidis by elisa technique in several selected flocks of laying hens

2004 ◽  
Vol 58 (3-4) ◽  
pp. 319-326 ◽  
Author(s):  
Maja Velhner ◽  
Dusan Orlic ◽  
Dubravka Potkonjak ◽  
Milos Kapetanov ◽  
Sava Lazic
Keyword(s):  
Antibody Titre ◽  
Salmonella Enteritidis ◽  
Laying Hens ◽  
Elisa Method ◽  
Specific Antibodies ◽  
Elisa Technique ◽  
High Level

In this paper, the antibody titre to Salmonella enteritidis (SE) was examined by the ELISA method in two flocks of laying hens, where during routine bacteriological investigations Salmonellae was never isolated, and in one flock where Colysepticemia was diagnosed and Salmonella isolated accidentally. In the flocks were Salmonellae were not isolated, a titre with a high level of specific antibodies to SE was discovered (15 and 45%), while the flock with accidental findings of SE was poorly positive (5%). These results point to the necessity of introducing serological monitoring to SE so that the infection of salmonella may be discovered early and the prevalence in the flock determined, and also for the purpose of applying adequate measures that could reduce the possibility of secretion of SE through eggs.

scholarly journals First serological record of Coxiella burnetii infection in the equine population of Slovakia

Biologia ◽  
2021 ◽  
Author(s):  
Monika Drážovská ◽  
Marián Prokeš ◽  
Boris Vojtek ◽  
Jana Mojžišová ◽  
Anna Ondrejková ◽  
...  
Keyword(s):  
16S Rrna ◽  
Coxiella Burnetii ◽  
Animal Species ◽  
Q Fever ◽  
Statistical Significance ◽  
Elisa Method ◽  
Zoonotic Pathogen ◽  
Specific Antibodies

AbstractCoxiella burnetii is a worldwide zoonotic pathogen causing Q fever in various animal species and humans. In Slovakia, cases of C. burnetii infection in both animals and humans are confirmed every year. The role of horses in the epidemiology of this neglected disease is still unclear. In our study, we focused on a serosurvey of C. burnetii in the equine population in Slovakia by the ELISA method. Subsequently, a nested PCR was performed to detect the 16S rRNA fragment of the genus Coxiella. Among 184 horse sera, the presence of specific antibodies to C. burnetii was detected in four samples, representing a 2.17% seropositivity. All the positive horses were mares; two originated from Central Slovakia and two from Eastern Slovakia. Although the number of positive samples was too small for a determination of statistical significance, our results provide the first confirmation of antibodies to C. burnetii in horses from Slovakia. Although no positive PCR result was obtained, these serological findings may help to clarify the circulation of the pathogen in the environment.

Determining the reproductive potential of repair heifers

2021 ◽  
pp. 43-47
Author(s):  
S. V. Fedotov ◽  
E. E. Oleinikova ◽  
S. G. Yakovlev ◽  
E. A. Mukha
Keyword(s):  
Blood Serum ◽  
Reproductive Potential ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Early Assessment ◽  
Hormonal Stimulation ◽  
Low Concentrations ◽  
High Level

Reproduction and culling of productive animals are the main factors that affect the number of cows in a herd. Timely determination of fertility in replacement heifers with subsequent culling of problem heifers will help reduce direct economic losses in breeding dairy cattle. When making a diagnosis, veterinarians should exclude all forms of temporary or permanent infertility, including Congenital anomalies (infantilism, freemartinism, deformities, etc.).In order to establish the hormonal status of heifers-freemartin in the blood serum, the concentration of sex hormones (progesterone, estradiol-17ᵦ, and testosterone) and anti-Müllerian hormone (AMH) was determined.Studies of the level of AMH using an enzyme-linked immunosorbent assay in blood serum have proven the possibility of early assessment of fertility in heifers. This method will allow early culling of Freemartin heifers from a productive herd. The study of AMH levels showed that its amount directly correlates with fertility in cattle, regardless of the period of the estrous cycle. When the concentration of AMH in the blood serum is below 100 pg / ml, heifers are not capable of fruitful insemination, while a high level of anti-Müllerian hormone (more than 380 ng / ml) makes it possible to successfully fertilize animals on the first attempt. All clinically healthy replacements obtained during a single pregnancy were successfully inseminated. At low concentrations of anti-Müllerian hormone, Freemartin heifers were sterile, even after hormonal stimulation (Co-Synx) and sperm doses were administered without clinical manifestation of sexual desire.The introduction of testing for the level of AMH by the ELISA method in the mandatory gynecological clinical examination of cattle in livestock enterprises is expedient and effective.

scholarly journals ELISA METHOD TO DETECT ABO BLOOD GROUP IN EXTERNAL SECRETION FLUIDS

2019 ◽  
Vol 2 (1) ◽  
pp. 31-38
Author(s):  
Abdul Halim Sadikin ◽  
Yefta Moenadjat ◽  
Novi Sylvia Hardiany
Keyword(s):  
Blood Group ◽  
Positive Reaction ◽  
Enzymatic Reaction ◽  
Abo Blood Group ◽  
Elisa Method ◽  
Blue Colour ◽  
Label Antibody ◽  
Elisa Technique ◽  
Blood Group Substances

Background: Usually it takes a large number of volume sample to determine blood group from external secretion fluids. But, in certain condition, samples are only available in very small amount. The objective of this study is to detect the presence of ABO blood group substances in mucosal fluid using ELISA technique, thus only requires small amount of samples.Objective: To develop an ELISA technique using the current anti-ABO antibodies for determination of blood group by hemagglutination technique and second peroxidase label antibody specific for mouse IgG, originally used for another ELISA technique.Methods: 100 μl of diluted human intestinal mucosal fluid were incubated overnight in 4oC in ELISA microplate wells, followed by addition anti-ABO antibodies. Then after incubation, a second revealing antibody anti mouse IgG labeled with peroxidase was added. After a brief incubation, substrate H2O2 and chromogenic TMB were added.Results: Positive reaction is marked by development of blue colour, which, on termination enzymatic reaction by addition 100 μl H2SO4 change to yellow.Conclusion: An ELISA method for detecting ABO substance in mucosal fluid can be developed from antibodies not specifically made for this technique, but specific only for the target.

Immediate implantation in a patient with chronic generalized parodontitis and apical granuloma

2019 ◽  
Vol 24 (2) ◽  
pp. 145-149
Author(s):  
A. I. Musienko ◽  
K. I. Nesterova
Keyword(s):  
Tooth Extraction ◽  
Tooth Root ◽  
Duration Of Treatment ◽  
Promising Direction ◽  
Aesthetic Result ◽  
Bone Atrophy ◽  
Alveolar Tissue ◽  
High Level ◽  
Immediate Implantation

Relevance. Rehabilitation of patients with moderate to severe generalized periodontitis is a leading problem in periodontology. It was the determination of the prospects for immediate implantation in patients with chronic periodontitis, combined with the pathology of the tooth root and maxillary sinus.Materials and methods. A group of 94 people with periodontitis and chronic odontogenic rhinosinus was observed who underwent sinus surgical treatment, tooth extraction and one-stage implantation with FRP growth factor according to the author's technology.Results. The method showed high efciency on the basis of assessing the clinical, aesthetic result and restoration of bone density after surgery.Conclusions. The developed technology is a promising direction, it allows to combine a high level of sanation of alveolar tissue with the advantages of immediate implantation, prevents bone atrophy, helps reduce the duration of treatment and the number of surgical and orthopedic interventions.

Methodical approaches to managing economic safety of tourism enterprises

2020 ◽  
pp. 160-164
Author(s):  
Leonid Tsubov ◽  
Oresta Shcherban
Keyword(s):  
Safety Management ◽  
Integrated Approach ◽  
Small Enterprise ◽  
Complex Nature ◽  
Efficient Management ◽  
Tourism Enterprises ◽  
High Level ◽  
Functional Components ◽  
Stimulation Control

The set of scientific-methodological tools to secure the mechanism of economic safety management of tourism entrepreneurship is examined as an aggregate of methods, tools, and conceptual activities directed at maintaining the high level of economic safety of tourism entrepreneurship. The features of managing the tourism enterprise and economic safety are analyzed. The basic valuation principles of the reliability and efficiency of the economic safety of the tourism enterprise are determined. The basic tasks of ensuring the economic safety of a small enterprise are outlined. The need to use the integrated approach that secures more opportunities to avoid threats and limits the danger of their emergence is emphasized. The most important principles for securing the economic safety of the tourism enterprise on the microeconomic level are described. Possible practical methods of risk management for the implementation of adopted decisions are proposed. The paper proves the fact that the complex nature of the management of the economic safety of the tourism enterprise and securing the sufficiently efficient management system of detecting and eliminating the threats are provided by the establishment of the management of the economic safety system of the tourism enterprise and its functional components. Research of the methodical approaches to the management of the tourism enterprises’ economic safety allows building and describing the functional structure of the mechanism of management of the tourist enterprise’s economic safety (it is formalized and described by 5 functions: determination of aims; planning; organization and adjusting; motivation and stimulation; control and monitoring).

scholarly journals Study of Well Waters from High-Level Natural Radiation Areas in Northern Vietnam

2021 ◽  
Vol 18 (2) ◽  
pp. 469
Author(s):  
Van-Hao Duong ◽  
Thanh-Duong Nguyen ◽  
Miklos Hegedus ◽  
Erika Kocsis ◽  
Tibor Kovacs
Keyword(s):  
Natural Radionuclide ◽  
World Health ◽  
Natural Radiation ◽  
Lifetime Cancer Risk ◽  
Northern Vietnam ◽  
Health Organization ◽  
High Level ◽  
Radiological Hazard Indices ◽  
Well Waters

The determination of natural radionuclide concentrations plays an important role for assuring public health and in the estimation of the radiological hazards. This is especially true for high level radiation areas. In this study, 226Ra, 228Ra and 238U concentrations were measured in well waters surrounding eight of the high-level natural radiation areas in northern Vietnam. The 226Ra, 228Ra and 238U activity concentrations vary from <1.2 × 10−3–2.7 (0.46), <2.6 × 10−3–0.43 (0.07) and <38 × 10−3–5.32 Bq/L (0.50 of median), respectively. 226Ra and 238U isotopes in most areas are in equilibrium, except for the DT-Thai Nguyen area. The calculated radiological hazard indices are generally higher than WHO (World Health Organization) recommendations. Average annual effective dose and excess lifetime cancer risk values due to drinking well water range from to 130 to 540 μSv/year and 7.4 × 10−6 to 3.1 × 10−5, respectively.

Colorimetric Determination of Aklomide (2-Chloro-4-Nitrobenzamide) in Poultry Feed

1969 ◽  
Vol 52 (3) ◽  
pp. 438-441
Author(s):  
Glenn M George ◽  
A C Daftsios ◽  
Joseph L Morrison
Keyword(s):  
Nitro Group ◽  
Poultry Feed ◽  
Colorimetric Determination ◽  
Average Recovery ◽  
Low Level ◽  
Color Development ◽  
Titanium Trichloride ◽  
High Level

Abstract The coccidiostat aklomide is extracted from feed with methanol and assayed colorimetrically by reduction of the nitro group to anamine with titanium trichloride and subsequent color development with t he Bratton-Marshall reaction. Thirteen laboratories studied the method collaboratively on two levels of medicated feed. Overall average recovery was 106.5% of the oretical for the low level and 104.5% of the oretical for the high level. The method is recommended for adoption as official first action

scholarly journals Effects of Essential Oils-Based Supplement and Salmonella Infection on Gene Expression, Blood Parameters, Cecal Microbiome, and Egg Production in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 360
Author(s):  
Georgi Yu. Laptev ◽  
Elena A. Yildirim ◽  
Larisa A. Ilina ◽  
Valentina A. Filippova ◽  
Ivan I. Kochish ◽  
...  
Keyword(s):  
Egg Production ◽  
Salmonella Enteritidis ◽  
Laying Hens ◽  
Expression Profiles ◽  
Blood Parameters ◽  
Post Inoculation ◽  
Production Traits ◽  
Immunological Parameters ◽  
Specific Expression ◽  
Blood Biochemical

One of the main roles in poultry resistance to infections caused by Salmonella is attributed to host immunity and intestinal microbiota. We conducted an experiment that involved challenging Lohmann White laying hens with Salmonella Enteritidis (SE), feeding them a diet supplemented with an EOs-based phytobiotic Intebio®. At 1 and 7 days post-inoculation, the expression profiles of eight genes related to immunity, transport of nutrients in the intestine, and metabolism were examined. Cecal microbiome composition and blood biochemical/immunological indices were also explored and egg production traits recorded. As a result, the SE challenge of laying hens and Intebio® administration had either a suppressive or activating effect on the expression level of the studied genes (e.g., IL6 and BPIFB3), the latter echoing mammalian/human tissue-specific expression. There were also effects of the pathogen challenge and phytobiotic intake on the cecal microbiome profiles and blood biochemical/immunological parameters, including those reflecting the activity of the birds’ immune systems (e.g., serum bactericidal activity, β-lysine content, and immunoglobulin levels). Significant differences between control and experimental subgroups in egg performance traits (i.e., egg weight/number/mass) were also found. The phytobiotic administration suggested a positive effect on the welfare and productivity of poultry.

scholarly journals Revealing the constituents of Egypt’s oldest beer using infrared and mass spectrometry

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mohamed A. Farag ◽  
Moamen M. Elmassry ◽  
Masahiro Baba ◽  
Renée Friedman
Keyword(s):  
Large Scale ◽  
Beer Brewing ◽  
Chemical Signatures ◽  
Chemical Determination ◽  
Materials Used ◽  
High Level ◽  
Beer Production ◽  
First Time ◽  
Detailed Chemical

Abstract Previous studies have shown that the Ancient Egyptians used malted wheat and barley as the main ingredients in beer brewing, but the chemical determination of the exact recipe is still lacking. To investigate the constituents of ancient beer, we conducted a detailed IR and GC-MS based metabolite analyses targeting volatile and non-volatile metabolites on the residues recovered from the interior of vats in what is currently the world’s oldest (c. 3600 BCE) installation for large-scale beer production located at the major pre-pharaonic political center at Hierakonpolis, Egypt. In addition to distinguishing the chemical signatures of various flavoring agents, such as dates, a significant result of our analysis is the finding, for the first time, of phosphoric acid in high level probably used as a preservative much like in modern beverages. This suggests that the early brewers had acquired the knowledge needed to efficiently produce and preserve large quantities of beer. This study provides the most detailed chemical profile of an ancient beer using modern spectrometric techniques and providing evidence for the likely starting materials used in beer brewing.

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