scholarly journals Isolation of Arcanobacterium haemolyticum from calves with pneumonia and proposal of a diagnostic protocol

2012 ◽  
Vol 66 (3-4) ◽  
pp. 165-174
Author(s):  
Ljiljana Suvajdzic ◽  
Jelena Asanin ◽  
Bjanka Lako ◽  
Aleksandar Potkonjak ◽  
Vladimir Sakac ◽  
...  
Keyword(s):  
Respiratory Infections ◽  
Isolation And Identification ◽  
Diagnostic Protocol ◽  
Beta Hemolytic Streptococcus ◽  
Routine Work ◽  
Biochemical Testing ◽  
Camp Test ◽  
Arcanobacterium Haemolyticum ◽  
Cultural Similarity ◽  
Arcanobacterium Pyogenes

The aims of this study are the isolation and identification of possible bacteriological agents in respiratory infections of calves and the optimization of a diagnostic protocol to identify Arcanobacterium haemolyticum. Lesions of lungs from calves with pneumonia were examined. Cultural, morphological and conventional biochemical testing were done. The investigation was complemented by the double CAMP test. Five strains of Arcanobacterium haemolyticum in pure culture were found. The presence of Arcanobacterium haemolyticum in the lungs of calves with pneumonia was established and, consequently, more attention should be paid to this species in everyday laboratory work. The cultural similarity of Arcanobacterium haemolyticum to common bacteria like beta-hemolytic Streptococcus spp. and Arcanobacterium pyogenes is probably responsible for rare reports on the isolation of Arcanobacterium haemolyticum in veterinary microbiology. Our results indicate that Arcanobacterium haemolyticum could be or is the etiological agent of pneumonia. Therefore, we suggest the diagnostic protocol available for routine work in most microbiological laboratories.

Three cases of Arcanobacterium pyogenes-associated soft tissue infection

2010 ◽  
Vol 59 (6) ◽  
pp. 736-739 ◽  
Author(s):  
Kannaiyan Kavitha ◽  
R. Latha ◽  
C. Udayashankar ◽  
K. Jayanthi ◽  
P. Oudeacoumar
Keyword(s):  
Wound Infections ◽  
Human Pathogen ◽  
Biochemical Tests ◽  
Closely Related Species ◽  
The World ◽  
Amoxicillin Clavulanic Acid ◽  
Camp Test ◽  
Arcanobacterium Haemolyticum ◽  
Arcanobacterium Pyogenes ◽  
Produce Acid

Arcanobacterium pyogenes is an established but often unrecognized human pathogen. A. pyogenes may also be misidentified as Arcanobacterium haemolyticum, which gives remarkably similar results in conventional biochemical tests. In this study, we have reported three cases of wound infections associated with A. pyogenes and also on the bacteriological characteristics which are relevant for identification of these isolates. The negative reverse CAMP test, the ability to produce acid from xylose and to hydrolyse gelatin and the positive β-glucuronidase test clearly differentiated A. pyogenes from other closely related species. All three isolates were uniformly susceptible to penicillin, ampicillin, amoxicillin–clavulanic acid, ceftriaxone and gentamicin, variably susceptible to tetracycline and erythromycin and uniformly resistant to cotrimoxazole. Only a few confirmed cases have been reported throughout the world and therefore the diagnostic evaluation of this organism is emphasized.

scholarly journals Urticaria caused by Arcanobacterium haemolyticum: Diagnostic and therapeutic failures

2006 ◽  
pp. 35-43
Author(s):  
Ljiljana Suvajdzic ◽  
Ekaterina Mrdja ◽  
Mirjana Bogavac ◽  
Ljubomir Dzambas
Keyword(s):  
Sore Throat ◽  
Streptococcus Pyogenes ◽  
Specific Pattern ◽  
Routine Laboratory ◽  
Laboratory Procedure ◽  
Penicillin Therapy ◽  
Cultural Traits ◽  
Camp Test ◽  
Arcanobacterium Haemolyticum ◽  
Probability Rate

We presented a case of seventeen-year-old girl exhibiting mild symptoms of sore throat, a marked urticarial rash and heavy desquamations of the skin on palms and soles. According to the antibiogram and diagnosis of Streptococcus non A non B group obtained in routine laboratory procedure, the patient was treated with penicillin; however, ineffectively. Escalation of urticaria and failure of the initial penicillin therapy shifted the diagnosis towards exanthema toxoalergicum and thus to treatment with corticosteroids and antihistamines, yet with no improvement. The culture was repeated with a new specimen. Diagnosis was made according to the specific pattern in a double CAMP test, and confirmed by the ApiCoryne diagnostic set (BioMerieux). With respect to cultural traits, the isolate mimicked Streptococcus pyogenes, yet developing specific pattern in a double CAMP test that directed our diagnosis towards coryneform microorganisms. The diagnosis of Arcanobacterium haemolyticum was confirmed with 99.9% probability rate and T = 0.75.

scholarly journals Deforming mandibular osteomyelitis in a cow caused by Trueperella pyogenes

2014 ◽  
Vol 44 (11) ◽  
pp. 2035-2038
Author(s):  
Karen Antas Caffaro ◽  
Carlos Alberto Hussni ◽  
Rafaela Mastrangelo Risseti ◽  
Daniel Queiroz França ◽  
Marília Masello Junqueira Franco ◽  
...  
Keyword(s):  
Bone Destruction ◽  
Radiographic Examination ◽  
Isolation And Identification ◽  
Antimicrobial Sensitivity ◽  
Caudal Portion ◽  
The Face ◽  
Mandibular Region ◽  
The Right ◽  
Arcanobacterium Pyogenes

This study reports an unusual case of deforming mandibular osteomyelitis in a cow caused by Trueperella (Arcanobacterium) pyogenes, on the face of the ventrolateral caudal portion of the right branch of the mandible. Fragment aspired of lesion by fine needle allowed cytological characterization, isolation and identification of T. pyogenes. Radiographic examination showed marked periosteal reaction in the right mandible, numerous lytic areas and cortical bone destruction. Despite of treatment based on in vitro antimicrobial sensitivity test, it was recommended the euthanasia due to progressive worsening of the cow's condition. Multiple abscesses were observed in the mandibular region at necropsy. Pyogranuloma was characterized in histological exam. Sampled material collected from the lesion after necropsy resulted in microbiological reisolation of T. pyogenes

Isolation and Identification of Listeria monocytogenes in Dairy Products

1988 ◽  
Vol 71 (3) ◽  
pp. 658-660 ◽  
Author(s):  
Joseph Lovett
Keyword(s):  
Listeria Monocytogenes ◽  
Nalidixic Acid ◽  
Incubation Period ◽  
Dairy Products ◽  
Isolation And Identification ◽  
Serological Tests ◽  
Isolation Medium ◽  
Enrichment Broth ◽  
Soft Cheese ◽  
Camp Test

Abstract After an outbreak of listeriosis in Massachusetts in 1983, the ability of Listeria monocytogenes to survive in raw and pasteurized milk was investigated. An enrichment broth (EB) containing acriflavine, nalidixic acid, and cycloheximide was used to eliminate overgrowth of the culture by competing organisms, and a modification of McBride's agar (MMA) was used as the isolation medium. The culture was incubated 24 h at 30°C. To isolate Listeria from soft cheese, the incubation period was lengthened to 1 week, and the EB culture was streaked to MMA at 1 and 7 days. Physical and biochemical patterns, the CAMP test, serological tests, and mouse pathogenicity studies were helpful in determining the identity of L. monocytogenes

ISOLATION AND IDENTIFICATION OF Vibrio parahaemolyticus FROM FLOWER CRAB (Portunus pelagicus) IN NEGERI SEMBILAN AND SELANGOR

2019 ◽  
Vol 7 (SI-TeMIC18) ◽  
Author(s):  
Nur Rabiatul Adawiah B Mohammad Noor ◽  
Sahilah Abd. Mutalib ◽  
Norrakiah Abdullah Sani
Keyword(s):  
Molecular Weight ◽  
Vibrio Parahaemolyticus ◽  
Agar Medium ◽  
Regulatory Gene ◽  
Virulence Gene ◽  
Testing Methods ◽  
Isolation And Identification ◽  
Portunus Pelagicus ◽  
Toxr Gene ◽  
Biochemical Testing

This study was conducted to determine the presence of Vibrio parahaemolyticus on 30 samples of flower crab (Portunus pelagicus) collected from Negeri Sembilan (n = 15) and Selangor (n = 15) from March to July 2014. A total of 193 isolates of Vibrio sp. were isolated from the thiosulphate citrate bile-salt (TCBS) agar medium. Of 79 Vibrio isolates were identified as V. parahaemolyticus through biochemical testing methods. In V. parahaemolyticus identification by detection of regulatory gene toxR, 51 (64.6%) of 79 isolates contained toxR gene while none of the isolates contained virulence gene of tdh dan trh. by targeting toxR gene which produced amplicons of 368 bp molecular weight. All V. parahaemolyticus isolates were negative for tdh and trh genes. Keywords: Vibrio parahaemolyicus, flower crab, toxR, trh, tdh

scholarly journals Isolation and Diagnosis of Pathogenic Bacteria from the Bowels of Local Chicken and Identifying Some of the Virulence Factors

2019 ◽  
Vol 12 (2) ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Virulence Factors ◽  
Pathogenic Bacteria ◽  
Klebsiella Pneumonia ◽  
Isolation And Identification ◽  
E Coli ◽  
Bacillus Spp ◽  
Local Chicken ◽  
Arcanobacterium Pyogenes

This study was conducted to the isolation and identification of bacteria from chicken intestine and livers in Mosul city. A total of 35 samples from intestine and 35 liver samples from local chicken were collected during a period from September 2018 to March 2019, the bacteria were diagnosed according to morphological, cultural and biochemical characteristics. The results showed (100%) positive to bacterial isolation for each samples of intestine and liver, (12) types of bacteria from (71) isolates for intestine, while (10) types from (36) isolates for liver. E. coli formed the highest percentage of intestinal isolates (28.16%), while Corynebacterium spp formed the highest percentage in liver isolates (33. 33%).The bacterial types were isolated from intestine included: E. coli (28.16%) Corynebacterium spp (25.35%), Enterococcus faecalis (15.49%), Klebsiella pneumonia (8.45%), Bacillus spp (7.04%), Proteus spp (5.63%), Staphylococcus aureus (4.22%), Lactobacillus spp (2.81%), Arcanobacterium pyogenes (1.4%), Citrobacter spp (1.4%). The bacterial types were isolated from liver included: Corynebacterium spp (33.33%), E.coli ( 19.44%), Staphylococcus aureus (16.6%), Bacillus spp (11.11%), Enterococcus faecalis (5.55%), Klebsiella pneumonia (5.55%), Pseudomonas aeruginosa (2.77%), Listeria monocytogenes (2.77%), Arcanobacterium pyogenes (2.77%). The bacterial types isolated from both intestine and liver were, E.coli, Corynebacterium spp, Staphylococcus aureus, Bacillus spp, Enterococcus faecalis ,Klebsiella pneumonia, Proteus spp and Arcanobacterium pyogenes. The virulence factors tests were used for some liver isolates which included, protease, licethinase, lipase, urease, coagulase and haemolysin.

scholarly journals Mycotic mastitis in sheep

2014 ◽  
Vol 13 (2) ◽  
pp. 1
Author(s):  
Biader Husain Hassan ◽  
Qassim Haleem Kshash
Keyword(s):  
Clinical Mastitis ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
California Mastitis Test ◽  
Candida Famata ◽  
Aspergillus Fumigates ◽  
Biochemical Testing ◽  
Yeasts And Molds ◽  
Penicillium Spp ◽  
Colony Shape

The study was aimed to investigate the mycotic mastitis in sheep during the period from October 2011 to May 2012 in different areas of Al-Diwaniya province. 253 ewes were examined, and from which 500 milk samples were collected (495 samples from apparently healthy ewes that examined by California Mastitis Test (CMT ) in addition to five samples from sheep infected with clinical mastitis) for isolation and identification of yeasts and molds adopted the method of culturing on Seaboard Dextrose and Corn meal agar in addition to Chrome agar and biochemical tests as well as specific yeast kits (Integral system yeast plus) for diagnosing the mycotic agents. Results indicated that the incidence of mastitis in ewes was 17.8%, while the percentage of mycotic mastitis was 9.4%, (0.4% and 9% of clinical and subclinical forms respectively). Yeasts were isolated and identified grossly by colony shape, size, and color, and by biochemical testing which represents 9.61% of the fungal causes, and the yeasts isolates were Candida famata and Rhodotorula rubra from the clinical cases of mastitis only. Molds 90.38% also were isolated, as a high isolate of Aspergillus niger 28.84%, Aspergillus flavus 23%, Aspergillus fumigates 17.30%, Penicillium spp 13.33%, Aspergillus terrus 5.76%, and the least percentage of isolation 3.84% was of the Fusarium spp.

scholarly journals Native nucleic acid electrophoresis as an efficient alternative for genotyping method of influenza virus.

2014 ◽  
Vol 61 (3) ◽  
Author(s):  
Beata Pajak ◽  
Krzysztof Lepek
Keyword(s):  
Influenza Virus ◽  
Respiratory Infections ◽  
Wide Spectrum ◽  
Genetic Material ◽  
Single Strand Conformation Polymorphism ◽  
Influenza Viruses ◽  
Nucleotide Polymorphisms ◽  
Virus Infections ◽  
Isolation And Identification ◽  
Number Of Patients

Influenza viruses are the worldwide major causative agents of human and animal acute respiratory infections. Some of the influenza subtypes have caused epidemics and pandemics among humans. The varieties of methods are available for the rapid isolation and identification of influenza viruses in clinical and environmental samples. Since nucleic acids amplification techniques such as RT-PCR have been adapted, fast and sensitive influenza type and subtype determination is possible. However, in some ambiguous cases other, more detailed assay might be desired. The genetic material of influenza virus is highly unstable and constantly mutates. It is known that single nucleotide polymorphisms (SNPs) results in resistance to commercially available anti-viral drugs. The genetic drift of the virus could also result in weakening of immune response to infection. Finally, in a substantial number of patients co-infection with various virus strains or types has been confirmed. Although the detection of co-infection or presence of minor genetic variants within flu-infected patients is not a routine procedure, a rapid and wide spectrum diagnostics of influenza virus infections could reveal an accurate picture of the disease and more importantly, is crucial for choosing the appropriate therapeutics and virus monitoring. Herein we present the evidences that native gel electrophoresis and MSSCP--a method based on multitemperature single strand conformation polymorphism could furnish a useful technique for minor variants, which escape discovery by conventional diagnostic assays.

A Simple Dichromatic Stain for Plastic Embedded Tissues

1970 ◽  
Vol 28 ◽  
pp. 296-297 ◽  
Author(s):  
G. R. Mackay ◽  
M. L. Mead
Keyword(s):  
Electron Microscopy ◽  
Toluidine Blue ◽  
Biological Material ◽  
Good Reproducibility ◽  
Routine Work ◽  
Staining Techniques

Color contrasting of 1 to 2 micron sections of plastic embedded biological material is an important adjunct to electron microscopy. The procedures in general use today are simple and rapid giving monochromatic results, e.g., toluidine blue. Although many di- and polychromatic histologic staining techniques have been modified to obtain a counterstaining effect with plasticembedded tissue, the methods are usually undesirable for routine work because they are time consuming, complicated and often defy good reproducibility.

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