The Un-Natural State

Author(s):  
BROCK THOMPSON
Keyword(s):  
Author(s):  
R. J. Narconis ◽  
G. L. Johnson

Analysis of the constituents of renal and biliary calculi may be of help in the management of patients with calculous disease. Several methods of analysis are available for identifying these constituents. Most common are chemical methods, optical crystallography, x-ray diffraction, and infrared spectroscopy. The application of a SEM with x-ray analysis capabilities should be considered as an additional alternative.A scanning electron microscope equipped with an x-ray “mapping” attachment offers an additional dimension in its ability to locate elemental constituents geographically, and thus, provide a clue in determination of possible metabolic etiology in calculus formation. The ability of this method to give an undisturbed view of adjacent layers of elements in their natural state is of advantage in determining the sequence of formation of subsequent layers of chemical constituents.


Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.


Author(s):  
Zhang zhaohua ◽  
Luo Dong ◽  
Guo Yisong

Since early 1970's the use of cold stage on SEM for observation of hydrated samples in their natural state has become more and more popular despite its high cost. Experiences gained from earlier experiments indicate that a successful design should incorporate thefollowing features:1. The specimen temperature should be below −135°C (the recrystallization point of water), lower the temperature, better the results.2. The frozen specimen, the cold block in the specimen preparation chamber, as well as the cold stage should be kept under vacuum at all times to keep them frost free.3. Different specimen preparation processes such as fracturing, coating and sublimation should be possible in one compact preparation chamber .


Author(s):  
O.S. Bezuglova ◽  

Rostov Region belongs to the highly protected natural territories characterized by the continuous plowing. There territories are the only reserves with the soils preserved in their natural state. However, these areas often lack detailed information about the soils quality and composition. Surveying soils on these territories is crucial for determination of their basic physical and chemical properties. The resulted compilation of soil maps could lay a foundation for creating the Red Book of Soils and the formation of a section in the soil-geographical database of the Russian Federation. Subsequently, such information can be used as a background data for the main types of soils in the region. It will be also valuable during monitoring and justification of conservation measures.


2010 ◽  
Vol 161 (8) ◽  
pp. 286-290
Author(s):  
Adrian Borgula

Where nature protection is concerned, the expert assessment of an environment poses questions about its natural and cultural heritage and its potential, about how representative it may be, about biodiversity in all its facets and about its development or potential threats to its existence. Although the forest was the dominant habitat in the original natural European landscape, for a long time nature protection has devoted too little attention to it. There are diverse threats and deficiencies. However, in the Swiss forests positive developments are discernible. Thus nature protection is one of the basic functions of the forest, nature reserves are being created, the area of the forest is increasing, as are reserves of deadwood, and greater importance is being given to regeneration suited to the site. Nevertheless much remains to be done to really achieve the goal of biodiversity and sus-tainability. For this purpose segregative and integrative measures are required. Since diversity needs surface area, in the first place it must be possible to set aside as forest reserves a quarter of the total forest area, spread over the whole country and preferably as natural forest reserves, in order to start the process of a long-term restoration to the natural state. Here the conservation of natural self-regulating processes supersedes classical nature protection. With a series of further integrative measures, biodiversity must also be promoted over the whole remaining forest area and in the areas of transition to other habitats. An abundance of varied structures is the key to diversity of species. A more courageous attitude to wilderness and “untidiness” when dealing with the forest is to be recommended. Nature experiences in wilderness areas give the population the possibility of discovering diversity and natural processes and encourage respect for the innumerable other species and habitats.


2021 ◽  
pp. 105678952199119
Author(s):  
Kai Yang ◽  
Qixiang Yan ◽  
Chuan Zhang ◽  
Wang Wu ◽  
Fei Wan

To explore the mechanical properties and damage evolution characteristics of carbonaceous shale with different confining pressures and water-bearing conditions, triaxial compression tests accompanied by simultaneous acoustic emission (AE) monitoring were conducted on carbonaceous shale rock specimens. The AE characteristics of carbonaceous shale were investigated, a damage assessment method based on Shannon entropy of AE was further proposed. The results suggest that the mechanical properties of carbonaceous shale intensify with increasing confining pressure and degrade with increasing water content. Moisture in rocks does not only weaken the cohesion but also reduce the internal friction angle of carbonaceous shale. It is observed that AE activities mainly occur in the post-peak stage and the strong AE activities of saturated carbonaceous shale specimens appear at a lower normalized stress level than that of natural-state specimens. The maximum AE counts and AE energy increase with water content while decrease with confining pressure. Both confining pressure and water content induce changes in the proportions of AE dominant frequency bands, but the changes caused by confining pressure are more significant than those caused by water content. The results also indicate that AE entropy can serve as an applicable index for rock damage assessment. The damage evolution process of carbonaceous shale can be divided into two main stages, including the stable damage development stage and the damage acceleration stage. The damage variable increases slowly accompanied by a few AE activities at the first stage, which is followed by a rapid growth along with intense acoustic emission activities at the damage acceleration stage. Moreover, there is a sharp rise in the damage evolution curve for the natural-state specimen at the damage acceleration stage, while the damage variable develops slowly for the saturated-state specimen.


Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 724
Author(s):  
Alberto Cesarani ◽  
Giuseppe Pulina

The concept of welfare applied to farm animals has undergone a remarkable evolution. The growing awareness of citizens pushes farmers to guarantee the highest possible level of welfare to their animals. New perspectives could be opened for animal welfare reasoning around the concept of domestic, especially farm, animals as partial human artifacts. Therefore, it is important to understand how much a particular behavior of a farm animal is far from the natural one of its ancestors. This paper is a contribution to better understand the role of genetics of the farm animals on their behavior. This means that the naïve approach to animal welfare regarding returning animals to their natural state should be challenged and that welfare assessment should be considered.


2021 ◽  
Vol 13 (9) ◽  
pp. 4928
Author(s):  
Alicia Vanessa Jeffary ◽  
Osumanu Haruna Ahmed ◽  
Roland Kueh Jui Heng ◽  
Liza Nuriati Lim Kim Choo ◽  
Latifah Omar ◽  
...  

Farming systems on peat soils are novel, considering the complexities of these organic soil. Since peat soils effectively capture greenhouse gases in their natural state, cultivating peat soils with annual or perennial crops such as pineapples necessitates the monitoring of nitrous oxide (N2O) emissions, especially from cultivated peat lands, due to a lack of data on N2O emissions. An on-farm experiment was carried out to determine the movement of N2O in pineapple production on peat soil. Additionally, the experiment was carried out to determine if the peat soil temperature and the N2O emissions were related. The chamber method was used to capture the N2O fluxes daily (for dry and wet seasons) after which gas chromatography was used to determine N2O followed by expressing the emission of this gas in t ha−1 yr−1. The movement of N2O horizontally (832 t N2O ha−1 yr−1) during the dry period was higher than in the wet period (599 t N2O ha−1 yr−1) because of C and N substrate in the peat soil, in addition to the fertilizer used in fertilizing the pineapple plants. The vertical movement of N2O (44 t N2O ha−1 yr−1) was higher in the dry season relative to N2O emission (38 t N2O ha−1 yr−1) during the wet season because of nitrification and denitrification of N fertilizer. The peat soil temperature did not affect the direction (horizontal and vertical) of the N2O emission, suggesting that these factors are not related. Therefore, it can be concluded that N2O movement in peat soils under pineapple cultivation on peat lands occurs horizontally and vertically, regardless of season, and there is a need to ensure minimum tilling of the cultivated peat soils to prevent them from being an N2O source instead of an N2O sink.


Author(s):  
Rasidnie Razin Wong ◽  
Zheng Syuen Lim ◽  
Noor Azmi Shaharuddin ◽  
Azham Zulkharnain ◽  
Claudio Gomez-Fuentes ◽  
...  

Diesel acts as a main energy source to complement human activities in Antarctica. However, the increased expedition in Antarctica has threatened the environment as well as its living organisms. While more efforts on the use of renewable energy are being done, most activities in Antarctica still depend heavily on the use of diesel. Diesel contaminants in their natural state are known to be persistent, complex and toxic. The low temperature in Antarctica worsens these issues, making pollutants more significantly toxic to their environment and indigenous organisms. A bibliometric analysis had demonstrated a gradual increase in the number of studies on the microbial hydrocarbon remediation in Antarctica over the year. It was also found that these studies were dominated by those that used bacteria as remediating agents, whereas very little focus was given on fungi and microalgae. This review presents a summary of the collective and past understanding to the current findings of Antarctic microbial enzymatic degradation of hydrocarbons as well as its genotypic adaptation to the extreme low temperature.


2021 ◽  
Vol 11 (6) ◽  
pp. 2605
Author(s):  
Giacomo Ferretti ◽  
Giulio Galamini ◽  
Evi Deltedesco ◽  
Markus Gorfer ◽  
Jennifer Fritz ◽  
...  

Using zeolite-rich tuffs for improving soil properties and crop N-use efficiency is becoming popular. However, the mechanistic understanding of their influence on soil N-processes is still poor. This paper aims to shed new light on how natural and NH4+-enriched chabazite zeolites alter short-term N-ammonification and nitrification rates with and without the use of nitrification inhibitor (DMPP). We employed the 15N pool dilution technique to determine short-term gross rates of ammonification and nitrification in a silty-clay soil amended with two typologies of chabazite-rich tuff: (1) at natural state and (2) enriched with NH4+-N from an animal slurry. Archaeal and bacterial amoA, nirS and nosZ genes, N2O-N and CO2-C emissions were also evaluated. The results showed modest short-term effects of chabazite at natural state only on nitrate production rates, which was slightly delayed compared to the unamended soil. On the other hand, the addition of NH4+-enriched chabazite stimulated NH4+-N production, N2O-N emissions, but reduced NO3−-N production and abundance of nirS-nosZ genes. DMPP efficiency in reducing nitrification rates was dependent on N addition but not affected by the two typologies of zeolites tested. The outcomes of this study indicated the good compatibility of both natural and NH4+-enriched chabazite zeolite with DMPP. In particular, the application of NH4+-enriched zeolites with DMPP is recommended to mitigate short-term N losses.


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