Glenoid Fossa Responses to Mandibular Lateral Shift in Growing Rats

Abstract Objective: To evaluate the morphological and histological responses of the glenoid fossa to mandibular lateral shift in growing rats. Materials and Methods: A resin plate was placed on the upper incisors of 4-week-old rats in the experimental groups to displace the mandible to the left during closure. The rats were killed after 2, 4, 8, and 12 weeks. The morphometric measurements were performed on dry skulls, and tissue blocks were processed for periodic acid and Schiff's reagent (PAS) staining to examine the new bone formation. Results: Gross measurements showed asymmetry in both the position and size of the fossae between the two sides after 4 weeks of lateral shift. The glenoid fossa on the ipsilateral side was repositioned relatively backward, outward and upward compared with the contralateral side and control group, whereas the fossa on the contralateral side was relocated relatively forward and downward compared with the control group. The length of the fossa was smaller on the ipsilateral side than on contralateral side and control group. At 2 weeks, the amount of newly formed bone in the posterior region of the fossa was higher in the experimental group than the control group. Conclusion: It is suggested that the mandibular lateral shift causes asymmetry in the position and size of the glenoid fossa and that this phenomenon can be related to different bilateral directional new bone formation in the posterior region.

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Effects of a Mandibular Lateral Shift on the Condyle and Mandibular Bone in Growing Rats

The Angle Orthodontist ◽

10.2319/091806-377 ◽

2007 ◽

Vol 77 (5) ◽

pp. 787-793 ◽

Cited By ~ 19

Author(s):

Chang Liu ◽

Sawa Kaneko ◽

Kunimichi Soma

Keyword(s):

Periodic Acid ◽

Growth Direction ◽

Contralateral Side ◽

Lateral Shift ◽

Mechanical Stimuli ◽

Ipsilateral Side ◽

Mandibular Bone ◽

Growing Rats ◽

Mandibular Asymmetry ◽

Condylar Head

Abstract Objective: To examine the effects of mandibular lateral shift on the growth and morphology of the condyle and mandibular bone in growing rats. Materials and Methods: A maxillary resin plate was designed to displace 4-week-old rat mandibles 2 mm to the left during closure. The plate was cemented, and the rats were killed after 2, 4, 8, or 12 weeks. Gross dimensions of the condylar head were measured. Radiographic films of the mandibles were exposed, and selected measurements were made. The newly formed bone in the condyle was evaluated with periodic acid and Schiff's reagent (PAS) staining. Results: The length of the condylar head was greater on the ipsilateral side compared with the contralateral side. The experimental rats developed an asymmetric mandible, shorter in horizontal dimension but longer in the vertical dimension on the ipsilateral side. The mandibular growth direction was also affected, with the mandible on the ipsilateral side growing in a more anterior and superior direction. The amount of newly formed bone was greater in the superior subchondral region but lower in the posterior subchondral region on the ipsilateral side compared with the contralateral side. Conclusions: Mechanical stimuli delivered by a functional shift produce a series of morphological and histological responses in the condyle and lead to condylar and mandibular asymmetry in rats.

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Neutralized Dicalcium Phosphate and Hydroxyapatite Biphasic Bioceramics Promote Bone Regeneration in Critical Peri-Implant Bone Defects

Materials ◽

10.3390/ma13040823 ◽

2020 ◽

Vol 13 (4) ◽

pp. 823 ◽

Cited By ~ 2

Author(s):

Hao-Hueng Chang ◽

Chun-Liang Yeh ◽

Yin-Lin Wang ◽

Kang-Kuei Fu ◽

Shang-Jye Tsai ◽

...

Keyword(s):

Bone Formation ◽

Bone Regeneration ◽

Bone Defects ◽

Dicalcium Phosphate ◽

Control Group ◽

New Bone Formation ◽

Control Groups ◽

Fast Bone ◽

And Control ◽

Bone Coverage

The aim of this study was to evaluate the efficacy of bone regeneration in developed bioceramics composed of dicalcium phosphate and hydroxyapatite (DCP/HA). Critical bony defects were prepared in mandibles of beagles. Defects were grafted using DCP/HA or collagen-enhanced particulate biphasic calcium phosphate (TCP/HA/Col), in addition to a control group without grafting. To assess the efficacy of new bone formation, implant stability quotient (ISQ) values, serial bone labeling, and radiographic and histological percentage of marginal bone coverage (PMBC) were carefully evaluated four, eight, and 12 weeks after surgery. Statistically significant differences among the groups were observed in the histological PMBC after four weeks. The DCP/HA group consistently exhibited significantly higher ISQ values and radiographic and histological PMCB eight and 12 weeks after surgery. At 12 weeks, the histological PMBC of DCP/HA (72.25% ± 2.99%) was higher than that in the TCP/HA/Col (62.61% ± 1.52%) and control groups (30.64% ± 2.57%). After rigorously evaluating the healing of biphasic DCP/HA bioceramics with a critical size peri-implant model with serial bone labeling, we confirmed that neutralized bioceramics exhibiting optimal compression strength and biphasic properties show promising efficacy in fast bone formation and high marginal bone coverage in peri-implant bone defects.

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The retrospective study of 93 patients with transmigration of mandibular canine and a comparative analysis with a control group

European Journal of Orthodontics ◽

10.1093/ejo/cjy067 ◽

2018 ◽

Vol 41 (4) ◽

pp. 390-396 ◽

Cited By ~ 4

Author(s):

Paweł Plakwicz ◽

Joanna Abramczyk ◽

Julita Wojtaszek-Lis ◽

Jolanta Sajkowska ◽

Barbara Warych ◽

...

Keyword(s):

Contralateral Side ◽

Permanent Teeth ◽

Control Group ◽

Study Group ◽

Affected Side ◽

Significant Difference ◽

Mandibular Canine ◽

And Control ◽

Primary Canine

Summary Objectives The aim of this study was to evaluate characteristics of patients with unilateral transmigration of a mandibular canine in the largest study group presented until now. Materials and methods The study group consisted of 93 patients with unilateral transmigration of mandibular canine; the control group included 85 non-affected patients. Type of transmigration, status of deciduous and permanent canines, prevalence of missing teeth, class of occlusion, and space conditions were assessed to draw comparisons between groups. Results In this study, 64.5 per cent patients presented type 1 of transmigration; types 2, 3, 4, and 5 were present in, respectively, 23.7, 5.4, 4.3, and 2.1 per cent patients. There was a clear, statistically significant difference (P < 0.0001) between the mean crown and apex migration and angulation for the three groups of canines (transmigrated, contralateral, and control), whereas no differences were observed for the total number of permanent teeth present. In the study group, 73.1 per cent patients retained their primary canine on the affected side and 18.3 per cent on the contralateral side; in the control group, 22.3 per cent subjects had at least one primary canine. There was a statistically significant difference in the distribution of types of malocclusion between the study and the control groups. Conclusions Transmigration of mandibular canine was associated with the presence of retained primary canine on the affected side, higher mesial tilting of contralateral mandibular canine when compared to the canines in the control group. Additionally, higher prevalence of Angle’s Class I occlusion in patients with canine transmigration was recorded.

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Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model

Materials ◽

10.3390/ma12101613 ◽

2019 ◽

Vol 12 (10) ◽

pp. 1613

Author(s):

Eunhye Jang ◽

Ja-Youn Lee ◽

Eun-Young Lee ◽

Hyun Seok

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Bone Regeneration ◽

Bone Morphogenetic Protein ◽

Human Bone ◽

Control Group ◽

New Bone Formation ◽

Morphogenetic Protein ◽

Regeneration Effect ◽

Recombinant Human Bone

The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (p = 0.004) and control groups (p = 0.000) and higher TbTh than the control group (p = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (p = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (p = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.

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Effect of functional lateral shift of the mandible on hyaluronic acid metabolism related to lubrication of temporomandibular joint in growing rats

European Journal of Orthodontics ◽

10.1093/ejo/cjaa012 ◽

2020 ◽

Vol 42 (6) ◽

pp. 658-663

Author(s):

Xiyuan Guo ◽

Ippei Watari ◽

Yuhei Ikeda ◽

Wu Yang ◽

Takashi Ono

Keyword(s):

Hyaluronic Acid ◽

Articular Cartilage ◽

Temporomandibular Joint ◽

Experimental Period ◽

Lateral Shift ◽

Control Group ◽

Condylar Cartilage ◽

Growing Rats ◽

Shift Group ◽

Recovery Group

Summary Background Hyaluronic acid (HA) is a major molecular component of the articular cartilage of the temporomandibular joint (TMJ) influencing joint lubrication. Functional lateral shift of the mandible (FLSM) can lead to malocclusion. This study investigated the effects of FLSM on HA metabolism and lubrication of the TMJ in growing rats. Methods Thirty 5-week-old male Wistar rats were divided into shift, recovery, and control groups. Rats in the shift and recovery groups were fitted with guiding plates to produce a 2-mm FLSM which were removed from the rats in the recovery group 14 days later. Animals were sacrificed at 14 and 28 days after the appliances were attached. Immunohistochemistry of HA-binding protein (HABP), hyaluronan synthase (HAS), and hyaluronoglucosaminidases (HYALs) was examined. Results The thickness of HABP-positively stained areas in the lateral regions in the bilateral condyle was reduced during the experimental period in the shift group compared with that in the control group. The proportion of HAS2-stained areas was bilaterally decreased in different regions of condylar cartilage during the experimental period in the shift group. The reduction of the HYAL2-stained area proportion in the condylar cartilage was more significant than that of HYAL1 at 14 days after appliance attachment in the shift group. HAS2 staining was not recovered in the recovery group. Limitations This research was based on animal experiments with a limited experimental period. Conclusion FLSM altered lubrication related HA metabolism in the articular cartilage of the TMJ in growing rats.

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Histological Comparison of New Bone Formation Using Amnion Membrane Graft Versus Resorbable Collagen Membrane: An Animal Study

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-16-00120 ◽

2018 ◽

Vol 44 (5) ◽

pp. 335-340 ◽

Cited By ~ 4

Author(s):

Soheil Koushaei ◽

Mohammad Hassan Samandari ◽

Sayed Mohammad Razavi ◽

Ahad Khoshzaban ◽

Shahriar Adibi ◽

...

Keyword(s):

Bone Formation ◽

Amniotic Membrane ◽

Clinical Impact ◽

Control Group ◽

Collagen Membrane ◽

New Bone Formation ◽

Histomorphometric Study ◽

Collagen Group ◽

Group 12 ◽

Amnion Membrane

The purpose of this article was to evaluate the bone induction effects of an amnion membrane–protected graft compared with a collagen membrane–protected graft in the repair of tibial bony defects in dogs. This study was performed using the tibial bone of dogs. After the removal of periosteum, similar holes were made with a 16-mm trephine drill (38 holes in total). For the study group, 10 holes were covered by absorbable collagen and 16 holes by amniotic membrane. In the control group, 12 holes were made and covered by the overlying soft tissue. Tibial bones were exposed after 6 and 12 weeks, and the samples were harvested and histologically processed. New bone formation was evaluated by histomorphometric study. Four Iranian mixed dogs older than 1.5 years were included in this study. The new bone formation was less in the control group when compared with the collagen group (P = .863). The collagen group showed less bone formation than the amnion group (P = .194), but this difference was not significant. However, bone formation in the amnion group was significantly more than in the control group (P = .050). Using the amniotic membrane appears to accelerate bone formation in guided bone regeneration. However, further studies should investigate its clinical impact on bone healing.

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Collagenated Synthetic Bone Substitute Material for Sinus Floor Elevation at Sites with a Perforated Schneiderian Membrane

Journal of Clinical Medicine ◽

10.3390/jcm9113764 ◽

2020 ◽

Vol 9 (11) ◽

pp. 3764

Author(s):

Sangyup Kim ◽

Jong-Hyuk Chung ◽

Seung-Yun Shin ◽

Seung-Il Shin ◽

Ji-Youn Hong ◽

...

Keyword(s):

Bone Formation ◽

Dimensional Stability ◽

Bone Substitute ◽

Control Group ◽

New Bone Formation ◽

Bone Substitute Material ◽

Sinus Floor Elevation ◽

Schneiderian Membrane ◽

Sinus Floor ◽

Substitute Material

Schneiderian membrane perforation (SMP) is the most common complication during sinus floor elevation (SFE). Conventional methods to repair SMP, such as using a collagen barrier, may be clinically demanding. The aim of the present study was to compare the effects of collagenated bone substitute materials with and without a collagen barrier to repair SMP during SFE in terms of new bone formation and dimensional stability. In 12 rabbits, intentional SMP was made during bilateral SFE. The rabbits were randomly assigned under two groups: the control group, in which the sinus was repaired with a collagen barrier, and the test group, in which the sinus was repaired without a collagen barrier. Collagenated bone substitute material was grafted in both groups. Healing periods of 2 weeks and 4 weeks were provided in both groups. There were no adverse clinical events. Histology revealed that the Schneiderian membrane had atrophied with loss of cilia and serous glands in both groups at 4 weeks. Histomorphometry revealed that the newly formed bone (test: 0.42 ± 0.17 mm2, control: 0.36 ± 0.18 mm2 at 2 weeks; test: 1.21 ± 0.36 mm2, control: 1.23 ± 0.55 mm2 at 4 weeks) or total augmented area did not significantly differ between the two groups at either time points (p > 0.05). In conclusion, collagenated bone substitute material without a collagen barrier demonstrated similar new bone formation and dimensional stability as that with a collagen barrier in repairing SMP.

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Influence of Homemade and Commercial Fibrin Sealant on Alveolar Bone Healing - an Experimental Study.

Blood ◽

10.1182/blood.v104.11.4110.4110 ◽

2004 ◽

Vol 104 (11) ◽

pp. 4110-4110

Author(s):

Maria Elvira P. Correa ◽

Oslei P. Almeida ◽

Danyel Peres ◽

Marcelo C. Alves ◽

Fernando F. Costa ◽

...

Keyword(s):

Bone Formation ◽

Bone Healing ◽

Fibrin Sealant ◽

Factor Xiii ◽

Alveolar Bone ◽

Healing Process ◽

Control Group ◽

Image Analysis System ◽

New Bone Formation ◽

Fibrin Sealants

Abstract The beneficial aspects of fibrin sealants for soft tissues are well documented, but studies of their direct influence on bone healing and their effectiveness in augmenting bone graft healing have produced conflicting results. The aim of this study was to evaluate the influence of fibrin sealants (FS) in the alveolar bone healing process during a period of up to 28 days. Seventy-five Wistar rats were submitted to a superior incisor extraction after intramuscular anesthesia (kentamine chloridrate 10% – 10mg/kg and tiazina chloridrate-5mg/kg). The rats were divided into tree different groups and the sealants were introduced into the alveolar bone. The first group (25) received human homemade fibrin glue, the second group (25) FS associated to factor XIII and aprotinin (Beriplast; Aventis-Beringher) and the third group (25) was the control. Animals were sacrificed by prolonged diethyl inhalation on days 7, 14 and 28 after surgery. Animal craniums were dissected and submitted to a decalcification, and preparated for H&E light microscopy. The morphometric study was performed by means of an interactive computerized image analysis system KS400 (Zeiss, Jena). New bone formation was carefully delimited in four different alveolar regions (apical, two middle areas and cervical) of each specimen. The data were statistically analyzed using multiple regression, ANOVA and Tukey test. Results showed that the amount of alveolar bone formation (μm) in the control group and commercial sealant was statistically similar. However, alveolus receiving homemade sealant presented less amount of new bone formation comparing to commercial sealant and control group (p=0.0034) (figure 1). The present study demonstrated that homemade fibrin sealant delays osteogenic formation. Commercial sealant did not improve alveolar repair however, the amount of new bone formation was slightly higher comparing to the control, probably due to the factor XIII present in this sealant. Figure Figure

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BAG S53P4 putty as bone graft substitute – a rabbit model

Biomedical glasses ◽

10.1515/bglass-2017-0003 ◽

2017 ◽

Vol 3 (1) ◽

Cited By ~ 1

Author(s):

Ilkka Saarenpää ◽

Patricia Stoor ◽

Janek Frantzén

Keyword(s):

Bone Formation ◽

Bone Regeneration ◽

Proximal Tibia ◽

Rabbit Model ◽

Bone Graft Substitute ◽

Control Group ◽

The Novel ◽

Bone Augmentation ◽

New Bone Formation ◽

New Zealand White Rabbits

AbstractBioactive glass (BAG) S53P4 granules represent a bone augmentation biomaterial for the surgical treatment of bony defects, even in challenging conditions such as osteomyelitis. The aim of this eight-week rabbit implantation study was to evaluate the biocompatibility and bone regeneration performance of a BAG S53P4 putty formulation following its implantation into the proximal tibia bone of twenty-eight New Zealand white rabbits. BAG S53P4 putty was compared to BAG S53P4 granules (0.5-0.8 mm) to evaluate whether the synthetic putty binder influences the bone regeneration of the osteostimulative granules. The putty formulation facilitates clinical use because of its mouldability, injectability and ease of mixing with autograft. Implantation of putty and granules into proximal tibia defects resulted in good osseointegration of the two groups. Both biomaterials were biocompatible, showed high new bone formation, high vascularization and periosteal growth. No signs of disturbed bone formation were observed due to the PEG-glycerol binder in the BAG S53P4 putty. Instead, intramedullary ossification and stromal cell reaction were more advanced in the putty group compared to the control group (p = 0.001 and p < 0.001). In conclusion, the novel mouldable BAG S53P4 putty showed reliable bone regeneration in bony defects without adverse tissue or cell reactions.

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Porous Titanium Membranes Combined with Various Graft Materials Induce Exophytic Bone Formation in Rabbit Calvaria

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.427 ◽

2006 ◽

Vol 309-311 ◽

pp. 427-432 ◽

Cited By ~ 3

Author(s):

Y. Kim ◽

Y.H. Kown ◽

J.B. Park ◽

J.H. Chung ◽

H.N. Lim ◽

...

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Porous Titanium ◽

Control Group ◽

Bovine Bone ◽

Graft Material ◽

New Bone Formation ◽

Freeze Dried ◽

Custom Made ◽

Human Cancellous Bone

The purpose of this study was to examine if the application of custom-made porous titanium membranes combined with bone graft materials promotes exophytic bone formation in rabbit calvaria. For this purpose, round decorticated calvaria sites were created using a round carbide bur. In the control group, rectangular parallelepiped-shaped porous titanium membranes (RPTMs) were placed on the decorticated sites and fixed with metal pins. In the experimental groups, RPTMs were filled with one of the following bone graft materials prior to fixing with metal pins: bovine bone mineral (BBM), demineralized freeze-dried human cortical bone (DFDB) or freeze-dried human cancellous bone (FDB). Animals were sacrificed at 8 and 12 weeks after surgery, and new bone formation was assessed by histomorphometric as well as statistical analysis. The results indicate that at 8 and 12 weeks, all the experimental groups demonstrated exophytic bone formation. At 12 weeks, DFDB group revealed the most new bone formation (p<0.05) and resorption of grafted materials (p<0.05). On the basis of these findings, we conclude that RPTMs may be used as an augmentation membrane for guided bone regeneration and DFDB as an effective bone-inducing graft material.

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