scholarly journals Antimicrobial Effect of Bacteriocin produced Pediococcus pentosaceus on some clinical isolates

2017 ◽  
Vol 27 (5) ◽  
pp. 26 ◽  
Author(s):  
Nehad A. Taher
Keyword(s):  
Antimicrobial Activity ◽  
Protein Concentration ◽  
Antimicrobial Effect ◽  
Clinical Isolates ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Pediococcus Pentosaceus ◽  
Gram Positive Bacteria ◽  
Agar Plug ◽  
Made In

About 10 isolates of Pediococcus sp were isolated from different cheese made in Iraq, These isolates were identified morphologically and biochemically and Api20 kit, thus there was only 6 isolate were identified as Pediococcus pentosaceus (60%).In this study, we investigate, the effect of crude Bacteriocin from Pediococcus pentosaceus on 30 clinical isolates (5 E.coli, 5 Klepsiella pneumoniae, 5 Staphylococcus aureus, 5 Pseudomonas aeroginosa, 5 Bacillus subtilis, 5 Candida albicans). The protein concentration of this Bacteriocin was measured 67mg\ml by Bradford method and used as (1:2) by vol during the measuring the antimicrobial activity against the above clinical isolates by two methods wells and  agar plug assay. The results showed that  the inhibitory activity of this Bacteriocin was higher by wells method than agar pluq assay against Gram–positive bacteria or Gram-negative bacteria and yeast under this study.

scholarly journals Comparative antimicrobial potential of Tribhuvana-Mishrana and its ingredients against clinical bacteria

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Vashisht Kiran ◽  
Thakur Vivek ◽  
Kumar Deepak ◽  
Sharma Khemchand
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Single Drug ◽  
Microbial Infections ◽  
Agar Well Diffusion Method

There are many single drug and compound formulations prescribed for Jwara (~ fever) in Ayurvedic classics. Tribhuvanakirti Rasa (TKR), Godanti Bhasma (GB) and Sudarshana Ghana Vati (SGV) are widely used compound formulations that are separately indicated for the treatment of Jwara (~fever) in different dosage schedules. In Ayurvedic Formulary of India Tribhuvana-Mishrana (TM) has been chiefly indicated in treatment of Sarva Jwara (~ fever). Therefore, in this study comparative antimicrobial activity of TM and its individual ingredients viz. TKR, GB and SGV were evaluated against four fever-causing microbes such as gram-positive bacteria Staphylococcus aureus and the gram-negative bacteria Salmonella typhii, Escherichia coli and Pseudomonas aeruginosa. The aqueous extract of all the four samples (formulation as whole and the three individual ingredients separately) in a dose 125mcg/ml were tested for their antimicrobial activity against four different microbes by agar well diffusion method. The study shows that TM and its ingredients independently show significant antimicrobial activity. S. aureus was found to be most sensitive to SGV and moderately sensitive to TKR and TM while S. typhii was found to be most sensitive to TM as compared to others. TKR was found to be most effective against E.coli while SGV and TM both showed significant antimicrobial effect against E. coli. TM could provide an alternative to synthetic antibiotics against human microbial infections.

scholarly journals Enaminone-Derived Pyrazoles with Antimicrobial Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Mashooq Ahmad Bhat ◽  
Mohamed A. Al-Omar ◽  
Ahmed M. Naglah ◽  
Abdul Arif Khan
Keyword(s):  
Antimicrobial Activity ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Initial Screening ◽  
Gram Positive ◽  
Gram Negative ◽  
Disc Diffusion Assay ◽  
Gram Positive Bacteria ◽  
Diffusion Assay

A series of pyrazoles derived from the substituted enaminones were synthesized and were evaluated for antimicrobial activity. All the compounds were characterized by the spectral data and elemental analysis. The synthesized compounds were initially screened for their antimicrobial activity against ATCC 6538, NCTC 10400, NCTC 10418, and ATCC 27853. During initial screening, compounds (P1, P6, and P11) presented significant antimicrobial activity through disc diffusion assay. These compounds were further evaluated for antimicrobial activity at different time points against Gram-positive and Gram-negative bacteria and presented significant activity for 6 hours. The activity was found to be greater against Gram-positive bacteria. In contrast at 24 hours, the activity was found only against Gram-positive bacteria except compound (P11), showing activity against both types of bacteria. Compound (P11) was found to have highest activity against both Gram-positive and Gram-negative bacteria.

Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Eti Nurwening Sholikhah ◽  
Maulina Diah ◽  
Mustofa ◽  
Masriani ◽  
Susi Iravati ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of  the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.

ANTIMICROBIAL ACTIVITY OF COPPER KAOLINITE AND SURFACTANT MODIFIED COPPER KAOLINITE AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

2016 ◽  
Vol 78 (3-2) ◽  
Author(s):  
Nor Syafawani Sarah Md Saad ◽  
Nik Ahmad Nizam Nik Malek ◽  
Chun Shiong Chong
Keyword(s):  
Antimicrobial Activity ◽  
Synergistic Effects ◽  
Wide Spectrum ◽  
Copper Nitrate ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray ◽  
Gram Positive Bacteria ◽  
Diffusion Technique

The aim of this research was to determine the antimicrobial activity of kaolinite modified with antimicrobial compounds against Gram positive and Gram negative bacteria. Copper kaolinite (Cu-kaolinite) was prepared by loading raw kaolinite with copper nitrate trihydrate (CuNO3) while surfactant modified Cu-kaolinite (SM-Cu-kaolinite) was prepared by adding cationic surfactants hexadecyltrimethyl ammonium (HDTMA) on Cu-Kaolinite. Samples was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analyzer. The antimicrobial activity of the samples was tested against Gram negative bacteria (Escherichia coli ATCC 11229 and Pseudomonas aeruginosa ATCC 15442), and Gram positive bacteria (Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212) through disc diffusion technique (DDT) and minimum inhibition concentration (MIC). The results showed that the antimicrobial activity of Cu-kaolinite increased after modified with HDTMA due to the synergistic effects of Cu ions and HDTMA molecules on the kaolinite. The antimicrobial activity for surfactant modified Cu-kaolinite was greater for Gram positive bacteria compared to Gram negative bacteria. In conclusion, the attachment of HDTMA on Cu-kaolinite contributed to the enhanced antimicrobial activity against wide spectrum of bacteria (Gram positive and Gram negative bacteria).

Antimicrobial Activity of Gentiana lutea L. Extracts

2009 ◽  
Vol 64 (5-6) ◽  
pp. 339-342 ◽  
Author(s):  
Katarina Šavikin ◽  
Nebojša Menković ◽  
Gordana Zdunić ◽  
Tatjana Stević ◽  
Dragoja Radanović ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Secondary Metabolites ◽  
Antimicrobial Effect ◽  
Gram Negative Bacteria ◽  
Synergistic Activity ◽  
Gram Negative ◽  
Gentiana Lutea ◽  
Methanolic Extracts ◽  
Pure Compounds

Methanolic extracts of flowers and leaves of Gentiana lutea L., together with the isolated compounds mangiferin, isogentisin and gentiopicrin, were used to investigate the antimicrobial activity of the plant. A variety of Gram-positive and Gram-negative bacteria as well as the yeast Candida albicans has been included in this study. Both extracts and isolated compounds showed antimicrobial activity with MIC values ranging from 0.12 - 0.31 mg/ml. Our study indicated that the synergistic activity of the pure compounds may be responsible for the good antimicrobial effect of the extracts. Quantification of the secondary metabolites was performed using HPLC

scholarly journals Increases in Hydrophilicity and Charge on the Polar Face of Alyteserin 1c Helix Change its Selectivity towards Gram-Positive Bacteria

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 238 ◽  
Author(s):  
Yamil Liscano ◽  
Constain H. Salamanca ◽  
Lina Vargas ◽  
Stefania Cantor ◽  
Valentina Laverde-Rojas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Solid Phase ◽  
Structural Characteristics ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Mutant Derivative ◽  
Conventional Antibiotics ◽  
Carboxy Terminal

Recently, resistance of pathogens towards conventional antibiotics has increased, representing a threat to public health globally. As part of the fight against this, studies on alternative antibiotics such as antimicrobial peptides have been performed, and it has been shown that their sequence and structure are closely related to their antimicrobial activity. Against this background, we here evaluated the antibacterial activity of two peptides developed by solid-phase synthesis, Alyteserin 1c (WT) and its mutant derivative (ΔM), which shows increased net charge and reduced hydrophobicity. These structural characteristics were modified as a result of amino acid substitutions on the polar face of the WT helix. The minimum inhibitory concentration (MIC) of both peptides was obtained in Gram-positive and Gram-negative bacteria. The results showed that the rational substitutions of the amino acids increased the activity in Gram-positive bacteria, especially against Staphylococcus aureus, for which the MIC was one-third of that for the WT analog. In contrast to the case for Gram-positive bacteria, these substitutions decreased activity against Gram-negative bacteria, especially in Escherichia coli, for which the MIC was eight-fold higher than that exhibited by the WT peptide. To understand this, models of the peptide behavior upon interacting with membranes of E. coli and S. aureus created using molecular dynamics were studied and it was determined that the helical stability of the peptide is indispensable for antimicrobial activity. The hydrogen bonds between the His20 of the peptides and the phospholipids of the membranes should modulate the selectivity associated with structural stability at the carboxy-terminal region of the peptides.

scholarly journals Amidoalkylation of heterocyclic amines by N-[5-(alkylsulfanyl)-1,3,4-thiadiazol-2-yl]- 2'-chloroacetamide and antimicrobial activity of derivatives

2021 ◽  
Vol 10 (4) ◽  
pp. 427-434 ◽  
Author(s):  
Turdibek Toshmurodov ◽  
Abdukhakim Ziyaev ◽  
Sobirdjan Sasmakov ◽  
Jaloliddin Abdurakhmanov ◽  
Mavluda Ziyaeva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Inhibition Zone ◽  
Heterocyclic Amines ◽  
Gram Negative Bacteria ◽  
In Vitro Screening ◽  
Gram Negative ◽  
Inhibition Zone Diameter ◽  
Gram Positive Bacteria ◽  
New Compounds

Amidoalkylation of secondary heterocyclic amines by N-[5-(alkylsulfanyl)-1,3,4-thiadiazol-2-yl]-2'-chloroacetamide resulted the new compounds 5-10 that contain 1,3,4-thiadiazole-5-thione moiety alongside pyperidine, morpholine, and cytisine fragments. In vitro screening of antimicrobial activity of synthesized compounds showed that N-[5-(amylsulfanyl)-1,3,4-thiadiazol-2-yl]-2'-morpholinacetamide exhibited an appreciable antibacterial activity against gram-negative bacteria of Escherichia coli (inhibition zone diameter of 16 mm) and gram-positive bacteria of Staphylococcus aureus and Bacillus subtilis (10-13 mm).

scholarly journals Novel 4-Thiazolidinone Derivatives as Anti-Infective Agents: Synthesis, Characterization, and Antimicrobial Evaluation

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Amit Gupta ◽  
Rajendra Singh ◽  
Pankaj K. Sonar ◽  
Shailendra K. Saraf
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Pseudomonas Fluorescens ◽  
Broad Spectrum ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Spectral Techniques ◽  
Fungal Strains ◽  
Gram Positive Bacteria ◽  
Antimicrobial Evaluation

A series of new 4-thiazolidinone derivatives was synthesized, characterized by spectral techniques, and screened for antimicrobial activity. All the compounds were evaluated against five Gram-positive bacteria, two Gram-negative bacteria, and two fungi, at concentrations of 50, 100, 200, 400, 800, and 1600 µg/mL, respectively. Minimum inhibitory concentrations of all the compounds were also determined and were found to be in the range of 100–400 µg/mL. All the compounds showed moderate-to-good antimicrobial activity. Compounds4a[2-(4-fluoro-phenyl)-3-(4-methyl-5,6,7,8-tetrahydro-quinazolin-2-yl)-thiazolidin-4-one] and4e[3-(4,6-dimethyl-pyrimidin-2-yl)-2-(2-methoxy-phenyl)-thiazolidin-4-one] were the most potent compounds of the series, exhibiting marked antimicrobial activity againstPseudomonas fluorescens,Staphylococcus aureus,and the fungal strains. Thus, on the basis of results obtained, it may be concluded that synthesized compounds exhibit a broad spectrum of antimicrobial activity.

Composition and Antimicrobial Activity of the Essential Oil of a Wild Kiwi

2011 ◽  
Vol 322 ◽  
pp. 160-163
Author(s):  
Yin Lu ◽  
Hong Chen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Fungal Species ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Leaf Oil ◽  
First Time

A medicinal wild kiwi in China, Actinidia valvata Dunn, has been well known for its activities against leprosy and cancers. The compositions and the antimicrobial activity of its leaf oil were reported for the first time. The oil obtained by hydrodistillation and analyzed by GC and GC-MS, was characterized by the high content of monoterpenes. Linalool (48.14%) is the major component identified, followed by 1,2-dimethyl-lindoline (7.94%), linolenic acid methylester (6.57%) and (E)-phytol (5.29%). The antimicrobial activity of the oil was evaluated against four bacterial and three fungal species. The results showed that it exhibited a mild antibacterial activity against two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), a significant activity against Gram-negative bacteria (Escherichia coli), and no activity on Pseudomonas aeruginosa. The test fungi were more sensitive to the oil, with a MIC range of 0.78~1.56 μL/mL than bacteria in the range which were significantly higher from 0.78 to 25.50 μL/mL.

Preliminary investigation of antimicrobial activity of the skin secretions of frogs from Bangladesh

2019 ◽  
Vol 40 (4) ◽  
pp. 531-536
Author(s):  
Md. Sirajul Islam ◽  
Md. Mokhlesur Rahman ◽  
Md. Mizanur Rahaman
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Preliminary Investigation ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Hoplobatrachus Tigerinus ◽  
Skin Secretions ◽  
Polypedates Leucomystax

Abstract Amphibians, like some animals and plants, defend themselves against various pathogenic organisms by producing and secreting various peptides and small molecules from granular skin glands. In this study, we evaluated for the first time, the antibiotic activity of the skin secretions of 8 different frog species (Euphlyctis cyanophlyctis, E. hexadactylus, Fejervarya teraiensis, F. asmati, F. syhadrensis, Hoplobatrachus tigerinus, Microhyla ornata and Polypedates leucomystax) from Bangladesh. These secretions were collected by a nonlethal approach through chemical stimulation and the antibacterial activity was evaluated by broth macrodilution method against some Gram-positive and Gram-negative bacteria. Our study revealed that all the skin secretions (8 out of 8) from the selected frogs have antimicrobial activity against Gram-positive bacteria and 7 out of 8 skin secretions possess antibacterial activity against Gram-negative bacteria tested. Further analysis of data showed that these secretions are significantly more effective against Gram-positive bacteria than Gram-negative bacteria.

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