EFFECT OF ECP ON THE STIMULATION OF LEUCOCYTE NUMBER AND PHAGOCYTOSIS OF JAPANESE EEL Anguilla japonica AGAINST Edwardseilla tarda NUF251

The extra cellular product (ECP) was rapidly induced leucocyte number compared to Edwardseilla tarda NUF251 in eel blood. The reason could be live cells need time to multiplied in eel body whereas the ECP composed of high protein therefore induce the leucocyte production in short time. The number of leucocyte in eel blood were not different between inactivated ECP and control eels. Although the leucocyte number increased gradually in live cells injected eel but have not correlation to increasing phagocytosis index (PI) in vivo phagocytosis. The PI and phagocytic rate (PR) of in vivo phagocytosis relatively constant from 24-96 h post injection.

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Corticotropin regulation of the synthesis of a specific rat adrenal cytosolic protein. Effects of hypophysectomy and actinomycin D

Biochemical Journal ◽

10.1042/bj1820717 ◽

1979 ◽

Vol 182 (3) ◽

pp. 717-725 ◽

Cited By ~ 7

Author(s):

Alice Dazord ◽

Dominique Gallet ◽

Helene Cohen ◽

Jose M. Saez

Keyword(s):

Protein Synthesis ◽

Cyclic Amp ◽

Total Protein ◽

Actinomycin D ◽

Polyacrylamide Gel Electrophoresis ◽

Cytosolic Protein ◽

And Control ◽

Corticotropin Stimulation ◽

Stimulation Of

The mechanism of corticotropin stimulation of the synthesis of a specific rat adrenal cytosolic protein was investigated. This protein (protein E) has a mol.wt. of approx. 30000. It is detected by polyacrylamide-gel electrophoresis of cytosol prepared from adrenal slices from rats treated with corticotropin in vivo and control rats, the slices being incubated with [3H]- and [14C]-leucine respectively. In rats 1–15 days after hypophysectomy, corticotropin, like dibutyryl cyclic AMP, induces an increase in protein E similar to that induced in control rats, even though both compounds no longer stimulate total protein synthesis. Corticotropin stimulation of protein E synthesis is mediated by cyclic AMP but not by corticosterone, since aminoglutethimide, a steroidogenic inhibitor, does not affect corticotropin stimulation, and dexamethasone alone has no effect. Actinomycin D, when injected in vivo 1h before or after corticotropin injection, prevents the effect of corticotropin on protein E synthesis, which is interpreted as evidence that mRNA synthesis is necessary for the stimulation of protein E synthesis. When injected more than 2h after corticotropin, actinomycin D does not prevent corticotropin stimulation of protein E synthesis, but completely blocks corticotropin stimulation of total protein synthesis. This is interpreted as meaning that, after stimulation of mRNA coding for protein E, corticotropin has no effect on the synthesis of protein E. On the other hand, corticotropin stimulation of protein E synthesis persists after hypophysectomy even though it no longer stimulates total protein synthesis. These data suggest that the factor(s) involved in the synthesis of protein E are more stable than those involved in total protein synthesis.

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Brown adipose tissue triacylglycerol synthesis in rats adapted to a high-protein, carbohydrate-free diet

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1999.276.4.r1003 ◽

1999 ◽

Vol 276 (4) ◽

pp. R1003-R1009 ◽

Cited By ~ 7

Author(s):

M. N. Brito ◽

N. A. Brito ◽

S. R. C. Brito ◽

M. A. F. Moura ◽

N. H. Kawashita ◽

...

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

High Protein ◽

Fourfold Increase ◽

Triacylglycerol Synthesis ◽

Brown Adipose ◽

Lipid Stores ◽

Glucose 6 Phosphate Dehydrogenase ◽

And Control

Adaptation of rats to a high-protein, carbohydrate-free (HP) diet induced a marked reduction of brown adipose tissue (BAT) fatty acid (FA) synthesis from both3H2O and [14C]glucose in vivo, with pronounced decreases in the activities of four enzymes associated with lipogenesis: glucose-6-phosphate dehydrogenase, malic enzyme, citrate lyase, and acetyl-CoA carboxylase. In both HP-adapted and control rats, in vivo incorporation of3H2O and [14C]glucose into BAT glyceride-glycerol was much higher than into FA. It could be estimated that most of the glycerol synthetized was used to esterify preformed FA. Glycerol synthesis from nonglucose sources (glyceroneogenesis) was increased in BAT from HP rats, as evidenced by an increased capacity of tissue fragments to incorporate [1-14C]pyruvate into glycerol and by a fourfold increase in the activity of phospho enolpyruvate carboxykinase activity, a key glyceroneogenic enzyme. The data suggest that high rates of glyceroneogenesis and of esterification of preformed FA in BAT from HP-adapted rats are essential for preservation of tissue lipid stores, necessary for heat generation when BAT is recruited in nonshivering thermogenesis.

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Identification of a retinoic acid-inducible gene I from Japanese eel (Anguilla japonica) and expression analysis in vivo and in vitro

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2016.05.036 ◽

2016 ◽

Vol 55 ◽

pp. 249-256 ◽

Cited By ~ 8

Author(s):

Jianjun Feng ◽

Songlin Guo ◽

Peng Lin ◽

Yilei Wang ◽

Ziping Zhang ◽

...

Keyword(s):

Retinoic Acid ◽

Expression Analysis ◽

Anguilla Japonica ◽

Japanese Eel ◽

Inducible Gene

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Using a Digital Twin of an Electrical Stimulation Device to Monitor and Control the Electrical Stimulation of Cells in vitro

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.765516 ◽

2021 ◽

Vol 9 ◽

Author(s):

Julius Zimmermann ◽

Kai Budde ◽

Nils Arbeiter ◽

Francia Molina ◽

Alexander Storch ◽

...

Keyword(s):

Electrical Stimulation ◽

Cellular Mechanisms ◽

Temperature Changes ◽

Digital Twin ◽

Stimulation Parameters ◽

Significant Temperature ◽

And Control ◽

Stimulation Of

Electrical stimulation for application in tissue engineering and regenerative medicine has received increasing attention in recent years. A variety of stimulation methods, waveforms and amplitudes have been studied. However, a clear choice of optimal stimulation parameters is still not available and is complicated by ambiguous reporting standards. In order to understand underlying cellular mechanisms affected by the electrical stimulation, the knowledge of the actual prevailing field strength or current density is required. Here, we present a comprehensive digital representation, a digital twin, of a basic electrical stimulation device for the electrical stimulation of cells in vitro. The effect of electrochemical processes at the electrode surface was experimentally characterised and integrated into a numerical model of the electrical stimulation. Uncertainty quantification techniques were used to identify the influence of model uncertainties on relevant observables. Different stimulation protocols were compared and it was assessed if the information contained in the monitored stimulation pulses could be related to the stimulation model. We found that our approach permits to model and simulate the recorded rectangular waveforms such that local electric field strengths become accessible. Moreover, we could predict stimulation voltages and currents reliably. This enabled us to define a controlled stimulation setting and to identify significant temperature changes of the cell culture in the monitored voltage data. Eventually, we give an outlook on how the presented methods can be applied in more complex situations such as the stimulation of hydrogels or tissue in vivo.

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Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166117 ◽

1996 ◽

Vol 54 ◽

pp. 730-731

Author(s):

E. D. Salmon ◽

J. C. Waters ◽

C. Waterman-Storer

Keyword(s):

Imaging System ◽

Ccd Camera ◽

Transmitted Light ◽

Microtubule Assembly ◽

Live Cells ◽

Optical Efficiency ◽

Multiple Band ◽

Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

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Genetic positioning of aquabirnavirus isolates from cultured Japanese eel Anguilla japonica in Korea

Diseases of Aquatic Organisms ◽

10.3354/dao02724 ◽

2014 ◽

Vol 109 (1) ◽

pp. 9-14 ◽

Cited By ~ 4

Author(s):

WS Kim ◽

MJ Oh

Keyword(s):

Anguilla Japonica ◽

Japanese Eel

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Stimulation of ovarian progesterone secretion by oxytocin in vivo

Acta Endocrinologica ◽

10.1530/acta.0.117s199-a ◽

1988 ◽

Vol 117 (4_Suppl) ◽

pp. S199-S200

Author(s):

E. DIETRICH ◽

K. RENTELMANN ◽

W. WUTTKE

Keyword(s):

Progesterone Secretion ◽

Stimulation Of

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CHROMATOGRAPHY OF THE 17-KETOGENIC STEROIDS IN THE DIAGNOSIS AND CONTROL OF CONGENITAL ADRENAL HYPERPLASIA

Acta Endocrinologica ◽

10.1530/acta.0.xxxiii0230 ◽

1960 ◽

Vol XXXIII (II) ◽

pp. 230-250 ◽

Cited By ~ 18

Author(s):

Eileen E. Hill

Keyword(s):

Congenital Adrenal Hyperplasia ◽

List Type ◽

Adrenal Hyperplasia ◽

Normal Children ◽

Paediatric Practice ◽

The Mean ◽

Cortisone Therapy ◽

And Control ◽

Clinical Problems ◽

Stimulation Of

ABSTRACT A method for the fractionation of the urinary 17-ketogenic steroids with no oxygen grouping at C11 and those oxygenated at C11, is applied to the clinical problems of congenital adrenal hyperplasia. In normal children the mean ratio of the non-oxygenated to oxygenated steroids is 0.24. In childrern with congenital adrenal hyperplasia the ratio is 2.3. The reason for this difference in ratio is discussed. The changes in ratio found under stimulation of the adrenal gland with exogenous or endogenous corticotrophin and the suppression with cortisone therapy are studied. This test can be applied to isolated samples of urine, a major advantage in paediatric practice, and can be carried out in routine laboratories. It is found to be reliable in the diagnosis and sensitive in the control of congenital adrenal hyperplasia.

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Experimental Study of the Impact of Alisma plantago-aquatica Secretions on Pathogenic Bacteria

Agricultural science and practice ◽

10.15407/agrisp1.03.003 ◽

2014 ◽

Vol 1 (3) ◽

pp. 3-7

Author(s):

O. Zhukorskyy ◽

O. Hulay

Keyword(s):

Pathogenic Bacteria ◽

Initial Density ◽

Biologically Active ◽

Erysipelothrix Rhusiopathiae ◽

Natural Focus ◽

Test Species ◽

Popula Tions ◽

And Control ◽

The Impact

Aim. To estimate the impact of in vivo secretions of water plantain (Alisma plantago-aquatica) on the popula- tions of pathogenic bacteria Erysipelothrix rhusiopathiae. Methods. The plants were isolated from their natural conditions, the roots were washed from the substrate residues and cultivated in laboratory conditions for 10 days to heal the damage. Then the water was changed; seven days later the selected samples were sterilized using fi lters with 0.2 μm pore diameter. The dilution of water plantain root diffusates in the experimental samples was 1:10–1:10,000. The initial density of E. rhusiopathiae bacteria populations was the same for both experimental and control samples. The estimation of the results was conducted 48 hours later. Results. When the dilution of root diffusates was 1:10, the density of erysipelothrixes in the experimental samples was 11.26 times higher than that of the control, on average, the dilution of 1:100 − 6.16 times higher, 1:1000 – 3.22 times higher, 1:10,000 – 1.81 times higher, respectively. Conclusions. The plants of A. plantago-aquatica species are capable of affecting the populations of E. rhusiopathiae pathogenic bacteria via the secretion of biologically active substances into the environment. The consequences of this interaction are positive for the abovementioned bacteria, which is demon- strated by the increase in the density of their populations in the experiment compared to the control. The intensity of the stimulating effect on the populations of E. rhusiopathiae in the root diffusates of A. plantago-aquatica is re- ciprocally dependent on the degree of their dilution. The investigated impact of water plantain on erysipelothrixes should be related to the topical type of biocenotic connections, the formation of which between the test species in the ecosystems might promote maintaining the potential of natural focus of rabies. Keywords: Alisma plantago-aquatica, in vivo secretions, Erysipelothrix rhusiopathiae, population density, topical type of connections.

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