scholarly journals Analisis E. Coli Pada Air Minum Dalam Kemasan Yang Beredar Di Kota Tasikmalaya

2019 ◽  
Vol 5 (2) ◽  
pp. 121-125
Author(s):  
Vita Meylani ◽  
Rinaldi Rizal Putra
Keyword(s):  
Drinking Water ◽  
Gamma Ray ◽  
Bottled Water ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Bottled Drinking Water ◽  
Living Things ◽  
Bacterial Content ◽  
Water Companies

Drinking water is the main need of humans as living things. Over time, humans are more practical so that for drinking needs they prefer to use bottled drinking water or refill drinking water. The high demand for bottled water raises the number of drinking water companies that issue bottled drinking water products. However, the quality of bottled water still needs to be assessed because it is not through pasteurization or other processing. So the microbiological content remains to be investigated. This study aimed to determine the bacteriological content in bottled drinking water. This study uses the Most Probable Number (MPN) method to test its bacterial content and gamma ray radiation to test its radiosensitivity. The sample in this study was bottled drinking water of various brands circulating in the City of Tasikmalaya. Based on the research results obtained from 13 samples there is 1 sample containing E. coli which is code B1 with a total bacterial content of 7 cells per 100 ml. So it can be concluded L samples are known to contain E. coli as much as 1.9 x 105 cells per ml.

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Bacteriological Quality of Drinking Water in Mardan, Khyber Pakhtunkhwa, Pakistan

2015 ◽  
Vol 21 ◽  
pp. 1-6
Author(s):  
Javid Ali ◽  
Said Hassan ◽  
Dr Ziaurahman ◽  
Inayat Ur Rahman ◽  
Sadhair Abbas ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Fecal Coliforms ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Khyber Pakhtunkhwa ◽  
E Coli ◽  
Micro Organisms

The present study was aimed to isolate and identify micro-organisms load of drinking water of Mardan city, Khyber Pakhtunkhwa Pakistan. A total of 27 samples of drinking water were collected from different locations of the study area. Total Plate Count was determined by pour plate method, while total coliforms, total fecal coliforms and E. coli were determined by multiple tube fermentation method. Of the total collected samples, 17 (62.96%) samples were contaminated with either one or more than one type of microorganisms. The results of most probable number test showed that 13 (48.15%) samples were unsatisfactory. It was concluded that the water should be treated before consumption for drinking purpose. Regular assessment of the water quality is recommended as regular monitoring of the water quality for improvement not only prevents disease and hazards but also checks the water resources from becoming further polluted. ECOPRINT 21: 1-6, 2014DOI: http://dx.doi.org/10.3126/eco.v21i0.11897

scholarly journals IDENTIFICATION OF DEFECTS OF PET AND HDPE-BASED DRINKING WATER PACKAGING PRODUCTS

2020 ◽  
Vol 8 (1) ◽  
pp. 48-56
Author(s):  
S Pupon ◽  
M Nugraha ◽  
Y Febryani ◽  
RS Tasnim ◽  
U Lestari ◽  
...  
Keyword(s):  
Drinking Water ◽  
Bottled Water ◽  
Molding Process ◽  
Profit Margins ◽  
The Third ◽  
Bottled Drinking Water ◽  
Water Companies ◽  
Increasing Demand ◽  
Short Shot ◽  
Manufacturing Stage

Increased community mobility has an impact on increasing demand for packaged beverage products. Data from Aspadin (the association of bottled water companies) there are more than 1500 brands of bottled water (AMDK) in Indonesia and it is estimated that trade will continue to increase every year. However, the profit margins of bottled drinking water products are not classified as high, so producers must do efficiency. One form of efficiency that can be done is to reduce the waste of packaging production, where most bottled water is made of plastic. This study aims to identify several product defects that often occur during the production process of bottled water based on PET. The process of making plastic-based drinking water packaging is divided into 3 stages. First is to make a bottle preform, the second is the blow molding process, which is the process of making a whole package from a preform, and the third is the process of making a lid. The machines used in these stages are different and in each of the stages successfully classified types of product defects. During the preform manufacturing stage, black specks or contaminants, short shots, bubbles, and flash are found. At the stage of making the whole bottle includes, off center base, failed blowing, arising bracelet, rough parting line. The last defect in the process of making the cap includes excess material flash Short shot, bridge or ring break, leak centergate.

scholarly journals Comparison of the Amount of Escherichia Coli in Refilled Drinking Water at the Depot with Bottled Drinking Water

2021 ◽  
Vol 11 (5) ◽  
pp. 401-409
Author(s):  
Frisca Ronauli Batubara ◽  
Dame Joyce Pohan ◽  
Dora Elysia Octavia Pasaribu
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Positive Sample ◽  
Bottled Water ◽  
Clean Water ◽  
Pseudomonas Sp ◽  
E Coli ◽  
Bottled Drinking Water ◽  
Agar Media

Water needed for daily use is viable water that is clean and healthy. Due to limited access to clean water, people buy bottled water and refillable drinking water. It is necessary to do clinical research at the laboratory to determine whether the water condition is feasible or not for consumption. The purpose of this study was to determine whether or not there is E.coli bacteria in the refillable drinking water and bottled water. This study uses an experimental method. Samples were taken from 15 refillable drinking water depots in Kecamatan Rawalumbu Bekasi and 15 bottled water samples. The bacterias identification was made by planting the samples in Blood Agar media and EMB. The results showed four positive samples of E. coli bacteria (26.7%) in the refillable drinking water depot at Kecamatan Rawalumbu Bekasi and one positive sample of E. coli bacteria (6.7%) in bottled water. It was also found that other types of bacteria such as Coliform (6.7%), Pseudomonas sp. (40%), Klebsiella sp. (13.3%), Staphylococcus sp. (6.7%), and Streptobacillus Gram + (13.4%). Key words: Refillable Drinking Water, Bottled Water, Escherichia coli.

scholarly journals Identifikasi Bakteri Escherichia coli dalam Air Minum Galon pada Kantin yang ada di Universitas Andalas Padang

2021 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Muhammad Rayhan Braja Gitawama ◽  
Netti Suharti ◽  
Nora Harminarti
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Data Analysis ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Drug Agency ◽  
Drinking Water Samples

The National Food and Drug Agency has controlled the refill drinking water in drinking water depots and also controlled food at school canteens ranging from elementary, junior high, to high school, but the National Food and Drug Agency never do food quality controlling in canteens at universities. Objectives: To identified the contamination by coliform and E. coli bacteria in drinking water at Andalas University canteen. Methods: This research was descriptive to identify coliform bacteria on  15 drinking water at the Andalas University faculty canteens.  Samples were taken directly using a sterile bottle, while data analysis using Most Probable Number  (MPN)  tables 5-1-1 and the presence of E. coli bacteria colonies from drinking water samples. Results: 9 of 15 water samples were contaminated by coliform bacteria with the highest MPN index of 240/100 ml that was found in 2 samples. From 9 samples containing coliform bacteria, all of them were found to contain E. coli bacteria. Conclusion: Most of the samples were contaminated by coliform and E. coli bacteria. Drinking water served using a kettle was more contaminated than drinking water served using gallons.Keywords: Coliform, Escherichia coli, MPN

scholarly journals Assessment of the Escherichia coli pollution in drinking water and water sources in Sistan, Iran

2017 ◽  
Vol 8 (3) ◽  
pp. 386-392
Author(s):  
Kambiz Nazemi ◽  
Saeed Salari ◽  
Majid Alipour Eskandani
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Potable Water ◽  
Water Sources ◽  
Most Probable Number ◽  
The West ◽  
Probable Number ◽  
Running Water ◽  
E Coli ◽  
West Part

Abstract Water should be free of dangerous agents and able to provide the nutritional needs of humans and animals. Adequate and reliable water supply is essential for healthy animal production. This study was carried out in Sistan region, Sistan and Baluchistan State, Iran, to evaluate the pollution rate of drinking water and water sources with fecal Escherichia coli via the most probable number (MPN) method. A total number of 100 water samples were collected and divided to drinking and non-potable water groups. The non-potable water was categorized according to flow type, irrigation usage, type of accessibility and geographic location. Mean number of MPN of fecal E. coli in non-potable and drinkable water were detected, 6.1 × 102 and 2.4 coliforms per 100 mL, respectively (p < 0.05). Running water was more contaminated than still and pipe water in the study area (p < 0.05). Significantly, the MPN of fecal coliforms, in non-potable water, collected from the east was higher than the west part of the study area (p < 0.05). Among potable water, in the study area, the west part was remarkably more polluted with fecal E. coli than the east part of the study area (p < 0.05). Results indicated the applicability of water for animal consumption and an alarming signal for human consumption and public health.

scholarly journals Kualitas Air Minum Yang Diproduksi Depot Air Minum Isi Ulang Di Kecamatan Bungus Padang Berdasarkan Persyaratan Mikrobiologi

2012 ◽  
Vol 1 (3) ◽  
Author(s):  
Rido Wandrivel ◽  
Netty Suharti ◽  
Yuniar Lestari
Keyword(s):  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Morbidity Rate ◽  
Probable Number ◽  
E Coli ◽  
Quality Of Water ◽  
Complete Test ◽  
Quality Water

Abstrak Latar belakang: Pendahuluan: Setiap depot air minum wajib melakukan pemeriksaan mutu produk sesuai dengan peraturan yang berlaku, namun tidak satupun dari sembilan depot air minum di Kecamatan Bungus melakukan hal tersebut. Diare adalah salah satu penyakit yang disebabkan oleh air minum yang tidak berkualitas. Morbiditas rate diare di Kecamatan Bungus tertinggi ke-2 di Kota Padang. Penelitian ini bertujuan untuk mengetahui kualitas air minum yang dihasilkan depot air minum di Kecamatan Bungus berdasarkan persyaratan mikrobiologi. Metode Penelitian: Populasi pada penelitian ini adalah air minum yang berasal dari depot air minum isi ulang di Kecamatan Bungus Padang dan sampel pada penelitian ini adalah seluruh populasi yang ada, yaitu sembilan sampel. Penelitian dilaksanakan dalam 2 tahap yaitu pengambilan sampel air dengan galon air sekaligus observasi faktor yang mempengaruhi kualitas air dan pemeriksaan mikrobiologis dengan Most Probable Number Test (MPN) terhadap sampel yang terdiri dari tiga tes, yaitu presumptive test, confirmative test, dan complete test. Hasil Penelitian: Lima dari sembilan sampel mengandung bakteri Coliform dan tiga dari lima sampel tersebut juga mengandung E. coli. Kesimpulan: Hal ini menunjukkan bahwa 55,6% depot air minum di Kecamatan Bungus menghasilkan air minum yang kualitasnya tidak memenuhi persyaratan mikrobiologi yang telah ditetapkan pemerintah. Beberapa faktor yang dapat mempengaruhi adalah air baku, kondisi depot, kebersihan operator, dan penanganan terhadap wadah pembeli. Kata kunci: Depot air minum isi ulang, pemeriksaan mikrobiologis Abstract Background: Every depot is obliged to do inspection of product quality prescribed by the regulations, but not one even also from nine drinking water depot in District of Bungus do the mentioned. Diarrhea is one of the disease that cause by bad quality water product. Diarrhea morbidity rate in the Bungus District is second highest in Padang City. This Research aim to to know the drinking water quality produced by drinking water refill depot in the Bungus District based on microbiological requirements. Methods: Population of this research is drinking water refill depots in the Bungus District of Padang city and sample of this research is all of existing population, that is nine drinking water depot. Research executed in 2 phase that is intake of water sample with gallon at the same time do observation of the factor that influencing the quality of water and mikrobiological test with Most Probable Number Test ( MPN) to the sampel which consist of three test, that is presumtive test, konfirmative test, and complete test. Result: Five from nine sample contain coliform bacteria and three from five the sampel also contain E. coli. Conclusion: This matter indicate that 55,6% drinking water refill depot in District of Bungus produce drinking water which its quality don’t fulfill microbiological requirements which have been specified by government. Some factor able to influence is standard water, condition of depot, hygiene of operator, and handling to place of buyer. Keywords: Drinking water refill depots, mikrobiological test

scholarly journals Escherichia Coli Content in Refill Drinking Water (AMIU) in Samatiga District, West Aceh Regency

2020 ◽  
Vol 7 (2) ◽  
pp. 6
Author(s):  
Azwar Azwar ◽  
Safrida Safrida ◽  
Yarmaliza Yarmaliza ◽  
Fitrah Reynaldi ◽  
Arfah Husna
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
World Health ◽  
Most Probable Number ◽  
Cross Sectional ◽  
Probable Number ◽  
E Coli ◽  
Escherichia Coli Bacteria

The quality of drinking water supply must be guaranteed. The WHO (World Health Organization) determines water quality by means of E. Coli bacteria. Based on the Minister of Health Regulation No. 492 / MENKES / PER / IV / 2010 The maximum permissible content of E. Coli in drinking water is 0 per 100 ml. The presence of E. coli in water is an indicator of human and animal fecal pollution that can cause diarrhea and even death. Selection of Refillable Drinking Water (AMIU) as an alternative to fulfill drinking water needs is a risk that can endanger health if the quality of AMIU is added if consumers do not pay attention to safety and hygiene. This study aims to see the content of E. coli in Refill Drinking Water (AMIU) in Samatiga District, West Aceh Regency. This research is experimental with cross sectional design using quantitative laboratory examination with the Most Probable Number (MPN) method. The population in this study were all refill drinking water depots that did not have a drinking certificate in Samatiga District, West Aceh Regency. The research sample used a total sampling method of 7 refill drinking water depots. The results of the analysis showed that from all samples as many as 6 samples (85.71%) tested positive for Escherichia coli bacteria and 1 sample (14.29%) tested negative for Escherichia coli bacteria. Based on the calculation of the number of colonies with the MPN / 100 ml table then comparing with the requirements of the Regulation of the Minister of Health of the Republic of Indonesia Number 492 / Menkes / Per / IV / 2010 concerning drinking water quality requirements that the 6 samples tested positive were not suitable for consumption.

Detection of Escherichia coli in Drinking Water Sources of Filter Units and Supply Water

2016 ◽  
Vol 19 (2) ◽  
pp. 206-210
Author(s):  
Arman Chowdhory ◽  
Nafisa Kabir ◽  
Md Mazharul Islam Chowdhury ◽  
Jakir Ahmed Chowdhury
Keyword(s):  
Drinking Water ◽  
Membrane Filtration ◽  
Tap Water ◽  
Pharmaceutical Journal ◽  
Water Sources ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Bacteriological Test ◽  
Positive Results

The identification of coliforms has been extensively used as an indicator of drinking water quality. The study was aimed to identify the presence or absence of E. coli from drinking water. Samples were collected from different filtration units and tap water sources of an institution in Dhaka city. All the samples were tested through presumptive, confirmed and completed bacteriological test through MPN (most probable number) using 3 sets of lactose broth (LB) media and MF (membrane filtration) technique using bacteriological filter and nutrient agar media to find out the CFU (colony forming unit). About 10% of samples showed positive results for E. coli in completed test, and also gave positive results in confirmed test and gram staining; having no more than 1100 MPN per 100 ml for samples of filter taps but the source water remained negative.Bangladesh Pharmaceutical Journal 19(2): 206-210, 2016

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