THE DEVELOPMENT OF THE METHOD TO IDENTIFY HONEY ADULTERATION

2020 ◽  
Author(s):  
Y.A. Balji ◽  
◽  
R.H. Mustafina ◽  
G.T. Ismagulova ◽  
◽  
...  
Keyword(s):  
Botanical Origin ◽  
New Methods ◽  
Sugar Syrup ◽  
Nectar Flow

The new methods for identification of honey adulteration is presented in the article. The method differs in reliability, speed and ease of application. There have been described the methods for evaluation of honey naturalness implemented in laboratories of veterinary and sanitary examination with their shortcomings. The method to identify honey adulteration has been developed, using the honey of different botanical origin (acacia, sunflower, rapeseed, fruticous, espartes, nectar flow, alpine, buckwheat, polyfloral). In total we used 72 samples of honey and conducted 162 laboratory researches. The diastatic number of honey was defined in accordance with All-Union Standard 19792-2001. The composition of artificial honey included sugar syrup and artificially inverted sugar

scholarly journals Antioxidant Activity as Biomarker of Honey Variety

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2069 ◽  
Author(s):  
Małgorzata Dżugan ◽  
Monika Tomczyk ◽  
Patrycja Sowa ◽  
Dorota Grabek-Lejko
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Laboratory Method ◽  
Botanical Origin ◽  
Hydrophobic Properties ◽  
Multivariate Statistical ◽  
Simple Method ◽  
Phenolics Content ◽  
Nectar Flow ◽  
Melissopalynological Analysis

Honey variety is commonly defined by beekeepers based on nectar flow availability and the only laboratory method to provide verification is the melissopalynological analysis. Therefore, a quick and simple method for accurate assessment of honey variety is still being researched. The aim of the study was to evaluate the antioxidant activity of honey as an indicator of variety through the use of multivariate statistical analysis. Materials for the study consisted of 90 samples of varietal Polish honeys (rape-12, tilia-10, goldenrod-11, dandelion-5, buckwheat-6, multifloral-17, nectar-honeydew-8 and coniferous honeydew-16 and leafy honeydew-5) obtained directly from apiaries. Honeys were investigated in aspect of antioxidant capacity by photochemiluminescence (PCL) methods using standard ACW and ACL kits. As the reference FRAP and DPPH methods were used. The total phenolics content (TPC) was determined through the Folin-Ciocalteu method. The strongest antioxidant activity was found for buckwheat, while the weakest was found for rape honeys regardless of the used method. Results of the used methods were positively correlated (r = 0.42 to 0.94). Analysis conducted by PCL method confirmed that the minor fraction of honey antioxidants exhibits hydrophobic properties. Clear separation of honey varieties using PCA and Clustering method indicate that antioxidant activity can be a useful parameter for determining the botanical origin of honey.

scholarly journals THE IMPACT OF TECHNOLOGICAL FACTORS OF HONEY ON ITS ADDITIONAL QUALITATIVE INDICATORS

2021 ◽  
pp. 42-50
Author(s):  
Е.А. МУРАШОВА ◽  
О.А. ФЕДОСОВА ◽  
О.В. СЕРЕБРЯКОВА
Keyword(s):  
Electrical Conductivity ◽  
Ph Value ◽  
Raw Materials ◽  
Federal State ◽  
Acidic Ph ◽  
Botanical Origin ◽  
Technological Factors ◽  
Sugar Syrup ◽  
Acacia Honey ◽  
The Impact

Проблема и цель. Целью работы стало исследование воздействия технологических факторов меда на показатель (рН) и электропроводность. Методология. Исследования проводили в испытательной лаборатории ФГБНУ «ФНЦ пчеловодства». Для определения рН и электропроводности у медов разного ботанического происхождения заготавливали пробы и исследовали их. Для проведения сравнительного анализа исследуемых показателей у натурального и фальсифицированных проб меда использовали образцы фальсификатов, которые были получены при скармливании пчелам сахарного сиропа, инвертного сиропа, крахмальной патоки, свекловичной патоки. Контрольные и опытные образцы заготавливали в десятикратной повторности. Результаты. На основании проведенных исследований было выявлено, что самое низкое (кислое) значение водородного показателя (в среднем 2,8 ед.рН) у акациевого меда. Каштановый мед имел значение 4,7 ед. рН. Мед из падевого сырья характеризовался менее кислой средой, чем остальные, в среднем 5,3 ед.рН. Наивысшей электропроводностью обладал падевый мед (0,7 мСм/см) и мед с каштана посевного (0,6 мСм/см). Самым кислым показателем рН среди фальсификатов характеризовался мед из сахарного сиропа (2,1 ед.рН). Щелочным значением рН обладал мед из свекловичной патоки 7,9 ед.рН. Наименьшая электропроводность выявлена у меда из сахарного сиропа 0,1 мСм/см. Наивысшей электропроводностью обладал фальсификат на основе крахмала – 0,8 мСм/см. Заключение. В ходе исследования достоверно установлено, что показатель концентрации водородных ионов в меде имеет зависимость от ботанического источника сырья меда, то есть от вида меда. Электропроводность имеет линейную зависимость от источника медосбора. Водородный показатель и электропроводность натурального меда и фальсификатов отличаются по значениям. Определение показателей рН и электропроводности может отражать фальсификацию медов. Problem and purpose. The aim of the work was to study the impact of technological factors of honey on pH and electrical conductivity. Methodology. The research was carried out in the testing laboratory of Federal State Budgetary Scientific Institution "Federal Research Center of Beekeeping". To determine pH and electrical conductivity of honey of different botanical origin, samples were collected and examined. To carry out a comparative analysis of the studied indicators in natural and falsified honey samples, which were obtained when feeding bees with sugar syrup, invert syrup, starch syrup, beet syrup. Control and experimental samples were harvested in tenfold repetition. Results. Based on the studies, it was revealed that the lowest (acidic) pH value was found in acacia honey, which averaged 2.8 pH units. Chestnut honey had a value of 4.7 pH units. Honey from honeydew raw materials was characterized by a less acidic environment than the rest, having on average 5.3 pH units. The highest electrical conductivity was possessed by honeydew honey (0.7 mS/cm) and chestnut honey (0.6 mS/cm). Honey from sugar syrup had the most acidic pH among counterfeits (2.1 pH units). Alkaline pH was found in honey from beet syrup (7.9 pH units). The lowest electrical conductivity was found in honey from sugar syrup (0.1 mS/cm). The starch-based counterfeit had the highest electrical conductivity (0.8 mS/cm). Conclusion. In the course of the study, it was reliably established that the indicator of the concentration of hydrogen ions in honey was dependent on the botanical origin of honey, or the type of honey. Electrical conductivity had a linear dependence on the origin of honey. The hydrogen index and electrical conductivity of natural honey and counterfeit products differed in values. PH and conductivity measurements can reflect honey falsifying.

scholarly journals Identification, characterization and evaluation of honeybee floras in Kafa, Sheka and Benchi Maji Zones of Southern Nations Nationalities and Peoples Region (SNNPR), Ethiopia

2021 ◽  
pp. 310-326
Author(s):  
Shegaw Tesfu ◽  
Giorgis Dawit Habte
Keyword(s):  
Focus Group ◽  
Adult Population ◽  
Field Observations ◽  
Botanical Origin ◽  
Focus Group Discussions ◽  
Group Discussions ◽  
Seasonal Performance ◽  
Source Plant ◽  
Croton Macrostachyus ◽  
Nectar Flow

The study was aimed with identifying and characterizing honeybee floras and their flowering seasons in south western parts of the country. Relevant data were collected through conducting questionnaire survey, focus group discussions, field observations and lab analysis of pollen and honey samples. In addition, seasonal performance of colonies in terms of brood area, adult population, pollen and nectar stores were evaluated. The obtained data were analyzed using SPSS-ver.20 and MsExcels. Accordingly, a total of 200floral species categorized under 77families identified. Of which, 26(13%) grouped under 16 families characterized as major bee floras. Seasons, January to February, July to August were considered as Dearth periods; March, September to October were brooding seasons, April, November to December were major nectar flow seasons. March to April and November to December were major honey flow seasons being the first for high land and mid land areas and the later for lowland areas whose major botanical origin was Schefflera abyssinica and Guizetia scabra respectively. However, in some areas with better forest covers, Manilkara butij become a major honey source plant. Whereas, January, June to July, March (in low lands) were considered as minor/mini harvesting seasons whose major botanical origin was Vernonia species, Croton macrostachyus and Combritum species respectively. It was noted that there was a significant correlation among brood area, adult population, pollen store and nectar stores at p<0.01.

Replication of Wet Objects and Cells

1974 ◽  
Vol 32 ◽  
pp. 102-103
Author(s):  
S. Basu ◽  
D. F. Parsons
Keyword(s):  
Water Vapor Pressure ◽  
High Vacuum ◽  
Polystyrene Latex ◽  
Vacuum System ◽  
Saturated Water Vapor ◽  
Replication Method ◽  
New Methods ◽  
Saturated Water ◽  
Water Vapors ◽  
Intermediate Chamber

We are approaching the invasiveness of cancer cells from the studies of their wet surface morphology which should distinguish them from their normal counterparts. In this report attempts have been made to provide physical basis and background work to a wet replication method with a differentially pumped hydration chamber (Fig. 1) (1,2), to apply this knowledge for obtaining replica of some specimens of known features (e.g. polystyrene latex) and finally to realize more specific problems and to improvize new methods and instrumentation for their rectification. In principle, the evaporant molecules penetrate through a pair of apertures (250, 350μ), through water vapors and is, then, deposited on the specimen. An intermediate chamber between the apertures is pumped independently of the high vacuum system. The size of the apertures is sufficiently small so that full saturated water vapor pressure is maintained near the specimen.

A Freeze Shadow Technique for the Preparation of Soft Paint Latexes for Electron Microscopy

1977 ◽  
Vol 35 ◽  
pp. 314-315
Author(s):  
Earl R. Walter ◽  
Glen H. Bryant
Keyword(s):  
Sample Preparation ◽  
High Vacuum ◽  
Vacuum System ◽  
Latex Particles ◽  
New Methods ◽  
Diffusion Pump ◽  
Film Forming ◽  
Routine Basis ◽  
Distribution Studies ◽  
Preparation Techniques

With the development of soft, film forming latexes for use in paints and other coatings applications, it became desirable to develop new methods of sample preparation for latex particle size distribution studies with the electron microscope. Conventional latex sample preparation techniques were inadequate due to the pronounced tendency of these new soft latex particles to distort, flatten and fuse on the substrate when they dried. In order to avoid these complications and obtain electron micrographs of undistorted latex particles of soft resins, a freeze-dry, cold shadowing technique was developed. The method has now been used in our laboratory on a routine basis for several years.The cold shadowing is done in a specially constructed vacuum system, having a conventional mechanical fore pump and oil diffusion pump supplying vacuum. The system incorporates bellows type high vacuum valves to permit a prepump cycle and opening of the shadowing chamber without shutting down the oil diffusion pump. A baffeled sorption trap isolates the shadowing chamber from the pumps.

Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

1990 ◽  
Vol 48 (3) ◽  
pp. 718-719
Author(s):  
Dai Dalin ◽  
Guo Jianmin
Keyword(s):  
Electron Microscope ◽  
Polyethylene Glycol ◽  
Chemical Reaction ◽  
Traditional Method ◽  
Osmium Tetroxide ◽  
Unsaturated Lipid ◽  
Embedding Method ◽  
Long Period ◽  
New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

Confronting the grey zone after severe brain injury

2019 ◽  
Vol 3 (6) ◽  
pp. 707-711 ◽  
Author(s):  
Andrew Peterson ◽  
Adrian M. Owen
Keyword(s):  
Brain Injury ◽  
Ethical Issues ◽  
Vegetative State ◽  
Clinical Care ◽  
Grey Zone ◽  
Severe Brain Injury ◽  
New Methods ◽  
Legal Decision Making ◽  
Technological Developments ◽  
The Brain

In recent years, rapid technological developments in the field of neuroimaging have provided several new methods for revealing thoughts, actions and intentions based solely on the pattern of activity that is observed in the brain. In specialized centres, these methods are now being employed routinely to assess residual cognition, detect consciousness and even communicate with some behaviorally non-responsive patients who clinically appear to be comatose or in a vegetative state. In this article, we consider some of the ethical issues raised by these developments and the profound implications they have for clinical care, diagnosis, prognosis and medical-legal decision-making after severe brain injury.

F. New Methods of Disinfection and Sterilization

2000 ◽  
Vol 12 (4) ◽  
pp. 380-380
Author(s):  
Kazuei Ogoshi ◽  
Taiji Akamatsu ◽  
Hiroyasu Iishi ◽  
Daizo Saito ◽  
Nobuhiro Sakaki ◽  
...  
Keyword(s):  
New Methods

New Methods Aim to Predict CKD Progression

2011 ◽  
Vol 44 (8) ◽  
pp. 14-15
Author(s):  
MARY ANN MOON
Keyword(s):  
Ckd Progression ◽  
New Methods

Perceptions of a Peer Suicide Prevention Program by Inmates and Professionals Working in Prisons

Crisis ◽  
2013 ◽  
Vol 34 (4) ◽  
pp. 289-292 ◽  
Author(s):  
Laurent Auzoult ◽  
Sid Abdellaoui
Keyword(s):  
Suicide Prevention ◽  
Prevention Program ◽  
Suicide Risk ◽  
Traditional Approach ◽  
Prevention Programs ◽  
New Methods ◽  
Penal Institutions ◽  
Peer Suicide ◽  
Suicide Prevention Programs ◽  
Methods Of Suicide

Background: Suicide prevention is a major challenge for penal institutions in many countries. The traditional approach relies on the expertise of health professionals and is supplemented by the intervention of other professionals and the inmates themselves. New methods of suicide prevention based on peer support have been developed in recent years. Peer prevention programs rely on the ability of inmates to identify suicide risk. Aims: This study examines perceived suicide risk among inmates and explores possible explanations. Method: 54 inmates and 17 professionals working in prisons responded to a questionnaire. Results: The peer prevention program was found to change inmates’ expectations of support in the event of a suicide crisis. The study also found that the inmates involved in the program tended to underestimate the risk of suicide. The perception of the prevention program and the level of self-consciousness were found to account for the underestimation of suicide risk. Conclusions: Support for inmates involved in suicide prevention programs must take into account their isolation in prison. The training provided to inmates must also consider the biases affecting the assessment of risk.

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