scholarly journals ANALYSIS OF IMMUNOHISTOCHEMICAL EXPRESSION OF P16INK4a IN NEOPLASTIC SQUAMOUS CELL LESIONS OF CERVIX

2016 ◽  
Vol 1 (02) ◽  
Author(s):  
Dr. K.R. Mohan ◽  
Dr. S. Sasikala ◽  
Dr. S. Ravi ◽  
Dr. P. Sagunthala, ◽  
Dr. I.Vijay Sathish Kumar,
Keyword(s):  
Squamous Cell ◽  
Immunohistochemical Analysis ◽  
Cost Effective ◽  
Large Cell ◽  
Hpv Infection ◽  
Invasive Squamous Cell Carcinoma ◽  
Strong Reaction ◽  
Tissue Samples ◽  
Reaction Intensity ◽  
P16 Expression

Introduction: Cervical cancer is caused by persistent infection by high risk Human papilloma virus (HPV).Increasing expression of HPV viral oncogenes might be reflected by increased expression of p16. Hence immunohistochemical detection of p16INK4a is an easy and cost effective method than molecular detection of HPV. Aims: The purpose of this study were to evaluate the results of expression of p16INK4a in neoplastic squamous cell lesions of cervix in order to assess the association of HPV infection in those lesions and to study the pattern of expression of p16 and also to compare p16 expression in various histological types of cervical neoplastic squamous cell lesions by immunohistochemistry. Methods: Immunohistochemical analysis of p16 expression was performed on 26 paraffin embedded tissue samples, obtained from cervical biopsy including 2 early invasive squamous cell carcinoma (SCC), 6 large cell keratinizing SCC, 16 large cell non- keratinizing SCC and 2 cases small cell non-keratinizing SCC by using commercially available mouse monoclonal antibody to p16 (clone G175 – 405). Two parameters were evaluated in p16 expression: Percentage of p16 positive cells and reaction intensity of p16 immunostaining. The p16 expression was graded as negative; Grade 1, 2, 3 and its reaction intensity was graded as negative, weak, moderate and strong. Results: In the present study out of 26 cases, the incidence of large cell non- keratinizing SCC constituted majority of the neoplastic lesions of cervix (61.5%). Most of the SCC (96.15%) showed grade 3 scoring for p16 positivity except one case which showed grade 2 scoring. Majority of SCC cases (96%) showed strong reaction intensity for p16 immunostaining. Conclusion: In this study of 26 neoplastic sqaumous cell lesions, all patterns of cervical neoplasia showed p16 positivity. P16 may be useful as an adjunct in histological sections to detect HPV in those lesions.

Immunohistochemical analysis of p16 expression, HPV infection and its prognostic utility in oral squamous cell carcinoma

2013 ◽  
Vol 42 (9) ◽  
pp. 676-681 ◽  
Author(s):  
Alexander Gröbe ◽  
Henning Hanken ◽  
Lan Kluwe ◽  
Maximilian Schöllchen ◽  
Silke Tribius ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Immunohistochemical Analysis ◽  
Hpv Infection ◽  
Prognostic Utility ◽  
P16 Expression

scholarly journals Nonsmoking and Nondrinking Oral Squamous Cell Carcinoma Patients: A Different Entity

2021 ◽  
Vol 11 ◽  
Author(s):  
Zhan Yang ◽  
Wei Du ◽  
Xu Zhang ◽  
Defeng Chen ◽  
Qigen Fang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Educational Background ◽  
Hpv Infection ◽  
Locoregional Control ◽  
Disease Stage ◽  
The Difference ◽  
P16 Expression

ObjectiveOur goal was to analyze the demographic and pathologic characteristics as well as prognosis in nonsmoking and nondrinking (NSND) oral squamous cell carcinoma (SCC) patients compared with typical oral SCC patients.Patients and MethodsA total of 353 patients were retrospectively enrolled and divided into two groups: the NSND group and the current smoking/current drinking (CSCD) group. Demographic, pathologic, and molecular data were compared between the two groups. The main research endpoints were locoregional control (LRC) and disease-specific survival (DSS).ResultsIn the NSND group, 16.3%, 41.9%, and 53.5% of patients were aged no more than 40 years, were female, and had an educational background of high school or above compared to 3.7%, 6.0%, and 38.2% of patients in the CSCD group, respectively. A total of 15.1% of the NSND patients had SCC of the lower gingiva and floor of the mouth, which was lower than the 35.6% of patients in the CSCD group. CSCD patients were likely to have an advanced disease stage (48.7% vs 32.5%, p=0.042) and poorly differentiated cancer (26.6% vs 16.3%, p=0.042). The NSND patients had a mean Ki-67 index of 24.5%, which was lower than the mean of 35.7% in the CSCD patients. The two groups had no HPV infection and similar p16 expression (4.7% vs 10.1%, p=0.132), but there was higher expression of p53 (38.6% vs 17.4%, p<0.001) and p63 (59.9% vs 29.1%, p<0.001) in the CSCD group. The 5-year LRC rates for NSND patients and CSCD patients were 48% and 38%, respectively, and the difference was significant (p=0.048). The 5-year DSS rates for NSND patients and CSCD patients were 56% and 39%, respectively, and the difference was significant (p=0.047). Further, a Cox model confirmed the independence of smoking and drinking status for affecting LRC and DSS.ConclusionNSND oral SCC patients are a different entity. HPV infection has a limited role in carcinogenesis in NSND patients, and p16 expression is associated with worse locoregional control.

scholarly journals Histopathological characteristics and coexpression of p53 and p16INK4a proteins in renal cancer

2008 ◽  
Vol 65 (11) ◽  
pp. 820-824
Author(s):  
Sladjana Zivkovic ◽  
Milos Kostov ◽  
Svetlana Pavlovic ◽  
Zaklina Mijovic
Keyword(s):  
Significant Positive Correlation ◽  
Renal Carcinoma ◽  
Malignant Tumors ◽  
Immunohistochemical Analysis ◽  
P53 Mutation ◽  
Clinicopathological Parameters ◽  
Tumor Differentiation ◽  
Tissue Samples ◽  
Protein P53 ◽  
P16 Expression

Background/Aim. Renal carcinoma represents histologically heterogeneous group of malignant tumors, with various clinical aggressiveness. The frequency of p53 mutation in primal renal carcinoma is rare, although there are information about its heterogeneous accumulation. The loss of protein p16 expression in primal renal carcinoma is detected in 20-30% of the cases. The aim of this paper was to determine frequency of mutated protein p53 and expression of protein p16INK4a in renal carcinoma, to analyze their correlative relation and relation with the examined clinicopathological parameters. Methods. The examination included 12 patients (66.7% men, 33.3% women), with patohistologically verified renal carcinoma. Expression of mutated form of protein p53 and protein p16 was determined in tissue samples, by immunohistochemical analysis using of mice monoclonical antibodies produced by DAKO, Denmark. Results. In 9 (75%) of the cases was detected mutated protein p53, of whom 66.6% had higher histological gradus of tumor (G3-4) and higher pathological stadium of the disease (pT3a-b) at the same time. In 7 (58.3%) and 5 (41.7%) of the cases expression of protein p16, the loss of expression of protein p16 were detected respectively. A statistically significant positive correlation was determined between pathological stadium of disease (TNM) and the degree of tumor differentiation (G) (? = 0.834; p < 0.001), as well as between TNM and mitotic index (? = 0.622; p = 0.031). Conclusion. A mutated form of protein p53 exists in 75% of the cases with the renal carcinoma and 66.6% of then have higher histological gradus of tumor and higher stadium of tumor disease at the same time. Coexpression of mutated protein p53 and protein p16INK4a in renal carcinoma is not statistically significant and it is not in correlation with clinicopathological parameters. Immunohistochemical analysis of mutated protein p53 in renal carcinoma can have predictive significance.

EGFR expression in invasive anal carcinoma.

2011 ◽  
Vol 29 (4_suppl) ◽  
pp. 412-412 ◽  
Author(s):  
A. A. Paliga ◽  
R. Onerheim ◽  
A. Gologan ◽  
A. Spatz ◽  
T. Vuong
Keyword(s):  
Head And Neck ◽  
Anal Cancer ◽  
Squamous Cell ◽  
Anal Carcinoma ◽  
Growth Factor Receptor ◽  
Staining Intensity ◽  
Hpv Infection ◽  
Epithelial Tumor ◽  
Tissue Samples ◽  
Egfr Expression

412 Background: Squamous cell anal carcinoma (SCAC) treatment remains unchanged since the institution of chemoradiation over 4 decades ago. Epidermal growth factor receptor (EGFR) is a protein often expressed in aggressive cancers and that is the target of the monoclonal antibody: cetuximab. Concurrent cetuximab and radiation has been particularly effective treating squamous cell carcinoma of the head and neck. Like head and neck cancer, anal cancer is an epithelial tumor of the alimentary tract that is radioresponsive and is associated with HPV infection. The rarity of this cancer limits its evaluation for biological markers. This study set out to thoroughly characterize EGFR expression by immunohistochemistry in 101 invasive SCAC tissue samples. Methods: One hundred and one pretreatment paraffin embedded invasive SCAC biopsies, obtained from the Montreal area between 1999 and 2009, were tested for EGFR expression by immunohistochemistry. All samples were confirmed to harbor invasive anal carcinoma on H&E slide preparations. Corresponding cancerous areas were identified on paraffin tissue blocks and cut out for tissue microarray analysis. Samples were immunostained with an EGFR antibody (clone SPM 341) on the Discovery XT Autostainer (Ventana), and staining was assessed by light microscopy by two pathologists. A semiquantitative combination score combining staining intensity with the percent of cells staining gave a final score: just detectable or weak (1+); moderate (2+); strong/intense (3+). Results: Of 101 patient biopsies, 82 samples had sufficient material for interpretation. Of these samples, 72/82 (90%) stained positive for EGFR, while 41/82 (50%) samples displayed at least moderate to strong staining. Conclusions: This is the largest cohort of SCAC tissue samples tested to date for EGFR expression and it confirms that the vast majority of invasive SCAC overexpress EGFR. EGFR likely plays a role in anal cancer tumor-genesis and progression. Testing of EGFR inhibitors in this patient population is justified. No significant financial relationships to disclose.

scholarly journals IMP3 and p16 expression in squamous cell carcinoma of the head and neck: A comparative immunohistochemical analysis

2017 ◽  
Vol 14 (2) ◽  
pp. 1665-1670 ◽  
Author(s):  
Marc-Oliver Riener ◽  
Josef Hoegel ◽  
Heinrich Iro ◽  
Arndt Hartmann ◽  
Abbas Agaimy
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Immunohistochemical Analysis ◽  
P16 Expression

scholarly journals Expression of p16 and Galectin3 in Squamous Cell Carcinoma of Uterine Cervix, in Relation to the Histomorphological Variants: A Cross-sectional Analysis

2021 ◽  
Author(s):  
Sarbashis Hota ◽  
Tushar Kanti Das
Keyword(s):  
Squamous Cell Carcinoma ◽  
High Risk ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Tumour Microenvironment ◽  
Hpv Infection ◽  
Tumour Grade ◽  
Cross Sectional ◽  
P16 Expression

Introduction: Cancer of uterine cervix comprises a big chunk of cancer registration worldwide. Now-a-days the immunohistochemical marker p16 has emerged as the surrogate marker of high risk Human Papilloma Virus (HPV) infection in cervical tissue. Galectin3, a ubiquitous agent likely to modulate different pro-survival properties necessary for neoplastic cells, is recently emerging as the guardian of tumour microenvironment. Aim: To study the expression of p16 and galectin3 in different histomorphological variants of cervical Squamous Cell Carcinoma (SCC) and their association with grade and stage. Materials and Methods: An observational cross-sectional study was undertaken in the Department of Pathology in a tertiary care hospital in East India, from January, 2019 to June, 2020. Fifty three samples diagnosed as invasive Squamous Cell Carcinoma (SCC) of uterine cervix were taken by systematic random sampling. Immunohistochemical examination was done using monoclonal antibodies against p16 and galectin3 after obtaining thin sections from formalin fixed paraffin embedded blocks and retrieval of antigen. The data was interpreted by light microscopy using a semiquantitative method with respect to prefixed parameters and statistical analysis was done by chi-square test and Fisher’s exact test using SPSS version 25.0. Results: Fifty two out of fifty three cases (98.1%) of squamous cell carcinoma in this study showed almost 100% block posivity of p16 in the tumour cells -strongly corroborative with high risk HPV infection. The non-keratinizing and the basaloid variant showed the strongest intensity of staining (3+). Only one case showed complete negativity of p16 expression. In galectin3 positive cases, strong expression of this marker is found in the invasive tongues of the tumour cells at the junction of tumour stromal interface, consistent with our knowledge regarding the importance of galectin3 in regulating the tumour microenvironment. The strongest galectin3 positivity(3+) was found in the single case of Lymphoepithelioma like squamous cell carcinoma and showed almost 100% positivity among the neoplastic cell population; whereas the non-keratinizing and Basaloid variant showed almost negative expression. Significant association (p=0.00021) found between tumour grade and p16 intensity. Conclusion: The non-keratinizing and basaloid variants of squamous cell carcinoma have shown statistically significant association with highest intensity of p16 staining along with diminished expression of galectin3. Increased tumour grade is also significantly associated with strong staining intensity of p16 and decreased galectin3 expression. However, no significant association is found between galectin3 expression or intensity of p16 expression and the stage of tumour.

Clinical Analysis of Human Papillomavirus Infection Types Distribution in Tissues of Cervical Squamous Cell Carcinoma

2020 ◽  
Vol 12 (7) ◽  
pp. 921-927
Author(s):  
Xiumei Zhang ◽  
Xianhai Zhu ◽  
Xiaolan Xu ◽  
Chengfu Sun ◽  
Jianxiang Geng ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Infection Rate ◽  
Squamous Cell ◽  
Eastern China ◽  
Cervical Squamous Cell Carcinoma ◽  
Hpv Infection ◽  
Squamous Cell Carcinomas ◽  
Hpv 16 ◽  
Tissue Samples

In this article, we investigated prevalence and distributed types of human papillomavirus (HPV) among female cervical squamous cell carcinoma patients in eastern China (mainly in Jiangsu Province). Tissue samples of total 1021 cases with cervical squamous cell carcinoma were incurred from female patients passing through biopsy or surgery. HPV DNA and genotypes of all subjects were examined using polymerase chain reaction (PCR) and gene-chip. There were 937 positive cases among 1021 cervical squamous cell carcinoma tissue samples with the total HPV infection rate of 91.77%. The HPV infection rate of single type was 75.42%. The predominant types of single infection with HPV were HPV 16 (52.60%), HPV 18 (5.19%), HPV 58 (4.51%), HPV 52 (2.84%), HPV 31 (2.74%), HPV 33 (2.45%), HPV 59 (1.76%) and HPV 45 (0.88%). The HPV infection rate of multiple types was 16.35%. The predominant types of multiple infection with HPV were HPV 16+18 (19.16%), 16+58 (6.59%),16+52 (5.99%), 16+33 (5.39%), 11+16 (2.99%), 16+31 (2.99%), 16+45 (2.99%), 16+42 (2.40%) and 16+59 (2.40%). Cervical squamous cell carcinomas had a close relationship with HPV infection. HPV 16, 18, 58, 52, 31, 33, 59, 45 with high prevalence in the cervical squamous cell carcinoma deserve great attention in eastern China (mainly in Jiangsu Province). The protection spectrum of divalent vaccine and ninevalent vaccine were, respectively, 68.52% (642/937) and 90.82% (851/937) in the cases of cervical squamous cell carcinomas.

scholarly journals Human papillomavirus DNA and p16 expression in head and neck squamous cell carcinoma in young French patients

2021 ◽  
Vol 49 (7) ◽  
pp. 030006052110225
Author(s):  
Chloé Molimard ◽  
Virginie L’Huillier ◽  
Alexis Overs ◽  
Christine Soret ◽  
Marie-Paule Algros ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Hpv Infection ◽  
Neck Squamous Cell Carcinoma ◽  
University Hospital ◽  
Hpv Dna ◽  
P16 Expression

Objectives Human papillomavirus (HPV) is a risk factor for head and neck squamous cell carcinoma (HNSCC), which is currently increasing worldwide. We evaluated the prevalence of HPV DNA and p16 expression in HNSCC patients age <45 years compared with patients aged ≥45 years. Methods Thirty-nine patients aged <45 years who presented at Besançon University Hospital with HNSCC since 2005 were included in this retrospective study. HPV DNA was detected by HPV genotyping and p16 expression was determined by immunohistochemistry using paraffin-embedded tissues. A matched-group of 38 patients aged ≥45 years from Besançon University Hospital was included. Results The overall prevalence of HPV infection was 11.7%. HPV16 was the only genotype detected in 4/39 and 5/38 patients, and p16 was expressed in 6/39 and 4/38 patients aged <45 years and ≥45 years, respectively. Conclusions HPV-positivity and p16 expression were similar in both age groups. The results suggest that p16 immunohistochemistry may provide a prognosis biomarker for all HNSCCs, not only oropharyngeal cancers, and this should be addressed in large clinical trials.

scholarly journals Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma

2016 ◽  
Vol 61 (6) ◽  
pp. 275-279
Author(s):  
O. Yu. Dvoryaninova ◽  
E. G. Nikitina ◽  
V. A. Bychkov ◽  
N. V. Litviakov
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Hpv Infection ◽  
Detection Methods ◽  
Quantitative Detection ◽  
Tissue Samples ◽  
Hpv Dna ◽  
Advantages And Disadvantages

The article describes methods for the human papillomavirus (HPV) detection in tumor and adjacent (morphologically intact) tissues of patients with laryngeal squamous cell carcinoma (LSSC) in terms of viral pathogenesis. Comparative evaluation of the principles and techniques for HPV detection was performed. Advantages and disadvantages of the HPV detection methods are described. Approaches for DNA and HPV oncoproteins E6-E7 identification are substantiated. The results of our research into the qualitative and quantitative detection of HPV in the tumor and adjacent tissues of patients with Lssc are described. The research was conducted using commercial test systems Amplisens HPV HR screen-titre-FL and Amplisens HPV HR genotype-FL. Based on these results we developed the algorithm of HPV detection in samples of tumor tissue of patients with Lssc. The need for typing HPV-positive tissue samples with low concentration of HPV DNA was discussed.

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