scholarly journals Production and Characterization of Scaffold Made of Hydroxyapatite and Pectin From Green Cincau Leaf (Premna Oblongifolia Merr)

INSIST ◽  
2018 ◽  
Vol 3 (1) ◽  
pp. 119
Author(s):  
Sudirman Habibie ◽  
Yesi Tristiyanti ◽  
Dwi Gustiono ◽  
Dachyar Effendi ◽  
Sri Yadial Chalid ◽  
...  
Keyword(s):  
Citric Acid ◽  
Cell Growth ◽  
Pore Size ◽  
Growth Medium ◽  
Bone Cell ◽  
Natural Polymers ◽  
3 Dimensional ◽  
Dimensional Matrix ◽  
Freeze Dryer

Scaffold is a 3-dimensional matrix created as a new bone cell growth medium made from natural polymers and bioceramics. The extracted pectin from green Cincau leaves (Premna oblongifolia Merr) and hydroxyapatite (HA) are used in the manufacture of scaffolds. Pectin was extracted using citric acid with variation concentration of 0, 0.1, 0.2 and 0.3% (w / v). The 3% (w / w) HA-pectin mixture, dried freeze using a freeze dryer. The characterization of extracted pectin and HA-pectin scaffold was then performed. The results showed that pectin of green Cincau leaves had low methoxyl content, which was 1.364 to 5.022%. The resulting scaffold has a pore size ranging from 8.25 to 115 µm while the scaffold resistance to the load, ie 0.03 to 0.15 MPa. The scaffold porosity that has been made is 15.33 to 40.97% while the density is 0.69 to 1.02 g/cm3.

Production and Characterization of Scaffold made of Hydroxyapatite and Pectin of Green Cincau Leaf (Premna Oblongifolia Merr)

2019 ◽  
Vol 1 (1) ◽  
pp. 13
Author(s):  
S Habibie ◽  
Y Tristiyanti ◽  
D Gustiono ◽  
M E Harahap ◽  
S Y Chalid ◽  
...  
Keyword(s):  
Citric Acid ◽  
Cell Growth ◽  
Pore Size ◽  
Growth Medium ◽  
Bone Cell ◽  
Natural Polymers ◽  
3 Dimensional ◽  
Dimensional Matrix

Scaffold is a 3-dimensional matrix created as a new bone cell growth medium madefrom natural polymers and bioceramics. The extracted pectin from green Cincau leaves(Premna oblongifolia Merr) and hydroxyapatite (HA) are used in the manufacture ofscaffolds. Pectin was extracted using citric acid with variation concentration of 0, 0.1,0.2 and 0.3% (w / v). The 3% (w / w) HA-pectin mixture, dried freeze using a freezedryer. The characterization of extracted pectin and HA-pectin scaffold was thenperformed. The results showed that pectin of green Cincau leaves had low methoxylcontent, which was 1.364 to 5.022%. The resulting scaffold has a pore size rangingfrom 8.25 to 115 µm while the scaffold resistance to the load, ie 0.03 to 0.15 MPa. Thescaffold porosity that has been made is 15.33 to 40.97% while the density is 0.69 to1.02 g/cm3Keywords: Green Cincau Leaf, Hydroxyapatite, Pectin, Scaffold

Determining Pore Structure and Growth Mechanisms in Templated Nanoporous Low-k Films

2005 ◽  
Vol 863 ◽  
Author(s):  
Hua-Gen Peng ◽  
Richard S. Vallery ◽  
Ming Liu ◽  
William E. Frieze ◽  
David W. Gidley ◽  
...  
Keyword(s):  
Pore Structure ◽  
Pore Size ◽  
Host Matrix ◽  
3 Dimensional ◽  
Nanoporous Films ◽  
Size Growth ◽  
Positronium Annihilation Lifetime Spectroscopy ◽  
The Relationship ◽  
Low K

AbstractTemplating is one of the most popular methods for generating nanocomposite and nanoporous films and the resultant pore size and pore interconnection length depend strongly on porogen concentration/porosity among other factors. Positronium Annihilation Lifetime Spectroscopy (PALS) analysis has been performed on a series of films produced using increasing concentrations of a type of cyclodextrin (CD) porogen in a modified silsesquioxane host matrix. PALS reveals the relationship between the resulting pore structure (both size and interconnection length) and porosity, which can be used to deduce pore shape. At low porogen concentration, isolated pores are resolved, but the pore size is consistent with a cluster of two or three CD molecules, rather than an individual one. As the porosity increases, the aggregation of the porogen domains appears to be more 3-dimensional (pseudo-random) with gradual increase in pore size. Computer simulations using a random pore growth model show consistent trends for pore size growth, but the agreement is poor for interconnection length. It is a key demonstration of the usefulness of PALS in untangling the fundamental pore structure and its evolution in porosity. PALS characterization of porosity provides novel feedback in the understanding and design of nanoporous materials.

CHARACTERIZATION OF PORE SIZE DISTRIBUTIONS USING NON-NEWTONIAN FLUIDS

2020 ◽  
Author(s):  
Scott C. Hauswirth ◽  
◽  
Majdi Abou Najm ◽  
Christelle Basset
Keyword(s):  
Pore Size ◽  
Newtonian Fluids ◽  
Size Distributions ◽  
Pore Size Distributions

scholarly journals Characterization of Phosphate Coatings: Influence of the Acid Pickling Conditions

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 1048
Author(s):  
Belén Díaz ◽  
X. Ramón Nóvoa ◽  
Carmen Pérez ◽  
Sheila Silva-Fernández
Keyword(s):  
Citric Acid ◽  
High Strength ◽  
Acid Pickling ◽  
Acid Cleaning ◽  
Inorganic Acids ◽  
Phosphate Coatings ◽  
Acid Bath ◽  
Coating Morphology ◽  
Coating Weight

This research emphasizes the importance of the acid cleaning prior to the phosphate development on high-strength steel rods. It compares the phosphate properties achieved after different acid-pickling conditions. The most common inorganic acids were considered in this study. Additionally, taking into account the environmental and safety concerns of these acids, the assessment of a less harmful organic acid is presented. This study revealed significant differences in the coating morphology and chemical composition whereas no great changes were found in terms of the coating weight or porosity. Thus, hydrochloric and sulfuric acid promote the growth of a Fe-enriched phosphate layer with a less conductive character that is not developed after the pickling with phosphoric acid. The phosphate developed after the citric acid pickling is comparable to that developed after the inorganic acids although with a porosity slightly higher. The temperature of the citric acid bath is an important parameter that affects to the phosphate appearance, composition, and porosity.

scholarly journals Structural Characterization of a Polysaccharide from Gastrodia elata and Its Bioactivity on Gut Microbiota

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4443
Author(s):  
Jiangyan Huo ◽  
Min Lei ◽  
Feifei Li ◽  
Jinjun Hou ◽  
Zijia Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Citric Acid ◽  
Gut Microbiota ◽  
Structural Characterization ◽  
Hot Water ◽  
Nmr Analysis ◽  
Gastrodia Elata ◽  
Hot Water Extraction ◽  
Hydroxybenzyl Alcohol

A novel homogeneous polysaccharide named GEP-1 was isolated and purified from Gastrodia elata (G. elata) by hot-water extraction, ethanol precipitation, and membrane separator. GEP-1, which has a molecular weight of 20.1 kDa, contains a polysaccharide framework comprised of only glucose. Methylation and NMR analysis showed that GEP-1 contained 1,3,6-linked-α-Glcp, 1,4-linked-α-Glcp, 1,4-linked-β-Glcp and 1,4,6-linked-α-Glcp. Interestingly, GEP-1 contained citric acid and repeating p-hydroxybenzyl alcohol as one branch. Furthermore, a bioactivity test showed that GEP-1 could significantly promote the growth of Akkermansia muciniphila (A. muciniphila) and Lacticaseibacillus paracasei (L.paracasei) strains. These results implied that GEP-1 might be useful for human by modulating gut microbiota.

scholarly journals Characterization of Polysaccharidic Associations for Cosmetic Use: Rheology and Texture Analysis

Cosmetics ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 62
Author(s):  
Giovanni Tafuro ◽  
Alessia Costantini ◽  
Giovanni Baratto ◽  
Stefano Francescato ◽  
Laura Busata ◽  
...  
Keyword(s):  
Raw Materials ◽  
Immersion Test ◽  
High Viscosity ◽  
Natural Polymers ◽  
Public Attention ◽  
First Choice ◽  
Wide Range ◽  
Textural Parameters ◽  
Complementary Techniques

As public attention on sustainability is increasing, the use of polysaccharides as rheological modifiers in skin-care products is becoming the first choice. Polysaccharide associations can be used to increase the spreading properties of products and to optimize their sensorial profile. Since the choice of natural raw materials for cosmetics is wide, instrumental methodologies are useful for formulators to easily characterize the materials and to create mixtures with specific applicative properties. In this work, we performed rheological and texture analyses on samples formulated with binary and ternary associations of polysaccharides to investigate their structural and mechanical features as a function of the concentration ratios. The rheological measurements were conducted under continuous and oscillatory flow conditions using a rotational rheometer. An immersion/de-immersion test conducted with a texture analyzer allowed us to measure some textural parameters. Sclerotium gum and iota-carrageenan imparted high viscosity, elasticity, and firmness in the system; carob gum and pectin influenced the viscoelastic properties and determined high adhesiveness and cohesiveness. The results indicated that these natural polymers combined in appropriate ratios can provide a wide range of different textures and that the use of these two complementary techniques represents a valid pre-screening tool for the formulation of green products.

scholarly journals Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1160
Author(s):  
Atul Pradhan ◽  
Nagaraja Mukkayyan ◽  
Kishor Jakkala ◽  
Parthasarathi Ajitkumar
Keyword(s):  
Cell Growth ◽  
Growth Medium ◽  
Differential Susceptibility ◽  
Physiological Significance ◽  
Glycerol Concentration ◽  
Diadenosine Tetraphosphate ◽  
Qpcr Data ◽  
Daughter Cells ◽  
Glycerol Level ◽  
Phase Population

Twenty to thirty percent of the septating mycobacterial cells of the mid-log phase population showed highly deviated asymmetric constriction during division (ACD), while the remaining underwent symmetric constriction during division (SCD). The ACD produced short-sized cells (SCs) and normal/long-sized cells (NCs) as the sister–daughter cells, but with significant differential susceptibility to antibiotic/oxidative/nitrite stress. Here we report that, at 0.2% glycerol, formulated in the Middlebrook 7H9 medium, a significantly high proportion of the cells were divided by SCD. When the glycerol concentration decreased to 0.1% due to cell-growth/division, the ACD proportion gradually increased until the ACD:SCD ratio reached ~50:50. With further decrease in the glycerol levels, the SCD proportion increased with concomitant decrease in the ACD proportion. Maintenance of glycerol at 0.1%, through replenishment, held the ACD:SCD proportion at ~50:50. Transfer of the cells from one culture with a specific glycerol level to the supernatant from another culture, with a different glycerol level, made the cells change the ACD:SCD proportion to that of the culture from which the supernatant was taken. RT-qPCR data showed the possibility of diadenosine tetraphosphate phosphorylase (MSMEG_2932), phosphatidylinositol synthase (MSMEG_2933), and a Nudix family hydrolase (MSMEG_2936) involved in the ACD:SCD proportion-change in response to glycerol levels. We also discussed its physiological significance.

Simulation and characterization of a dual-type 3-dimensional silicon detector

2012 ◽  
Vol 60 (11) ◽  
pp. 1862-1867
Author(s):  
Jihoon Choi ◽  
Kyungmin Lee ◽  
Eunil Won
Keyword(s):  
Silicon Detector ◽  
3 Dimensional ◽  
Type 3
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