Cetuximab alone has a dose-dependent antitumor effect in oral cavity cancer cells: an in vitro study

Aim: This research was to investigate the effects and mechanisms of HSYA in vascular endothelial injury by vitro study. Methods: Dividing HUVECs as Normal Control (NC), Model (LPS treated) group, HSYA-L, HSYA-M and HSYA-H groups. Cells in the HSYA treatment groups were treated with LPS, followed by 40 mg/ml, 80 mg/ml, and 120 mg/ml HSYA intervention (HSYA-L, HSYA-M, and HSYA -H groups), respectively. Measuring the cell proliferation, apoptosis, relative proteins and mRNA (TLR4, MyD88 and NF-κB(p65)) expressions by MTT, Flow cytometry, WB and RT-qPCR assay. Using cellular immunofluorescence to evaluate NF-κB(p65) nuclear volume of difference groups. Results: With HSYA supplement, the cell proliferation rates were significantly up-regulation with cell apoptosis significantly down-regulation with TLR4 relatived mRNA and proteins and NF-κB(p65) nuclear significantly depressed with dose-dependent (P <0.05, respectively). Conclusion: HSYA improved vascular endothelial injury induced by LPS via TLR4 pathway In Vitro.

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Targeted delivery of mesoporous silica nanoparticles loaded monastrol into cancer cells: an in vitro study

Applied Nanoscience ◽

10.1007/s13204-017-0593-8 ◽

2017 ◽

Vol 7 (8) ◽

pp. 549-555 ◽

Cited By ~ 5

Author(s):

Huzaifa Hanif ◽

Samina Nazir ◽

Kehkashan Mazhar ◽

Muhammad Waseem ◽

Shazia Bano ◽

...

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Cancer Cells ◽

Targeted Delivery ◽

Mesoporous Silica Nanoparticles ◽

In Vitro Study ◽

Vitro Study

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Effect of Vitamin D3on nonmelanoma skin cancer cells: A comparative in vitro study

International Journal of Growth Factors and Stem Cells in Dentistry ◽

10.4103/gfsc.gfsc_2_20 ◽

2020 ◽

Vol 3 (1) ◽

pp. 12

Author(s):

Shahram Ghanaati ◽

Eva Dohle ◽

Pasinee Vorakulpipat ◽

Sarah Al-Maawi ◽

Rita Schröder ◽

...

Keyword(s):

Skin Cancer ◽

Cancer Cells ◽

In Vitro Study ◽

Nonmelanoma Skin Cancer ◽

Vitro Study

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In Vitro Study of Calcium Microsecond Electroporation of Prostate Adenocarcinoma Cells

Molecules ◽

10.3390/molecules25225406 ◽

2020 ◽

Vol 25 (22) ◽

pp. 5406 ◽

Cited By ~ 1

Author(s):

Aleksander Kiełbik ◽

Wojciech Szlasa ◽

Olga Michel ◽

Anna Szewczyk ◽

Mounir Tarek ◽

...

Keyword(s):

Prostate Cancer ◽

Cancer Cells ◽

Calcium Binding ◽

In Vitro Study ◽

Vitro Study ◽

Dynamics Simulation ◽

Confocal Laser ◽

Prostate Cancer Cells ◽

Voltage Pulses

Electroporation, applied as a non-thermal ablation method has proven to be effective for focal prostate treatment. In this study, we performed pre-clinical research, which aims at exploring the specific impact of this so-called calcium electroporation on prostate cancer. First, in an in-vitro study of DU 145 cell lines, microsecond electroporation (μsEP) parameters were optimized. We determined hence the voltage that provides both high permeability and viability of these prostate cancer cells. Subsequently, we compared the effect of μsEP on cells’ viability with and without calcium administration. For high-voltage pulses, the cell death’s mechanism was evaluated using flow-cytometry and confocal laser microscopy. For lower-voltage pulses, the influence of electroporation on prostate cancer cell mobility was studied using scratch assays. Additionally, we applied calcium-binding fluorescence dye (Fluo-8) to observe the calcium uptake dynamic with the fluorescence microscopy. Moreover, the molecular dynamics simulation visualized the process of calcium ions inflow during μsEP. According to our results calcium electroporation significantly decreases the cells viability by promoting apoptosis. Furthermore, our data shows that the application of pulsed electric fields disassembles the actin cytoskeleton and influences the prostate cancer cells’ mobility.

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