scholarly journals Ameliorated Impact of Fenugreek Seed Extract on Some Blood Cellular and Biochemical Parameters in Female Albino Rats Exposed to Lead Acetate

Author(s):  
Rozhya Abduljalil Naser ◽  
Rabar Yousif Mameik ◽  
Nazar M. Shareef Mahmood ◽  
Kurdo Bapir Chato

Lead acetate (LA) is considered as the common hazardous compund present in our habitat. It has a several diverse-organ toxicant effect in various healthy issues included diseases of the hepatic, renal, and other body systems. Fenugreek seeds (Trigonella foenum graecum L.) were used in curing medicine and as food supplement since ancient times. After that, their antioxidant and hepatoprotective properties have been studied. Therefore the presented was based on their biological properties of this plant and conducted to examine the protective impact of fenugreek seed extract upon LA toxicity on some blood cellular and biochemical parameters of female albino rats (Rattus norvegicus). Twenty eight female rats were randomly and equally divided into four groups each one contain seven rats as following; first group received basal diet and tap water ad libitum. Second group received basal diet and LA 80 mg/L with water ad libitum. Third group received basal diet contained 2.5% fenugreek seed extract and LA 80 mg/L with water ad libitum. Fourth group received basal diet contained 2.5% fenugreek seed extract with water ad libitum. Rat’s administration was continued daily for two weeks respectively. The administration of rats with LA produced significant reduction in liver weight, food intake, while it increased triglyceride (TG), alkaline phosphatase (ALP), aspartate aminotransferase (AST), uric acid (UA) and creatinie (CR). While supplemented diet with 2.5% Fenugreek seed extract improved body weight, organ weight, lipid peroxidation, TG, ALP, and AST. Our results plead for the profiteering of fenugreek seeds as a dietary supplement, because it showed protective effect of their content in polyphenolic flavonoids, antioxidant and membrane-protective effects, against exposure to the LA compound to protect injurious risk.

2019 ◽  
Vol 29 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Lucie Widowati ◽  
Ani Isnawati ◽  
Sukmayati Alegantina ◽  
Fifi Retiaty

Abstract The proportion of stunting in children under 5 years old in Indonesia was approximately 30,8% in 2018. One of the things that is presumably related to the occurrence of stunting is lack of nutrition that can be attributed to exclusive breastfeeding. Fenugreek seeds have properties as laktogoga and moringa leaves in addition to having efficacy as lactagoga, also have advantage in nutritional content, particularly high protein content, groups of minerals and vitamins. The aim of the study was to assess the potential of the formula fenugreek seed extract (Trigonella foenum-graecum L) and moringa leaves (Moringa oleifera Lamk.) as a supplement to release milk while at the same having nutrient content. Methods using the test weighting method in pregnant female rats by measuring the volume of milk taken by baby rats. Test were carried out on five groups of mother rats namely groups than were given : fenugreek and moringa extract (1: 1) with different doses I, II and III; comparison group (moloco) and control group (distilled water). Each mother fed five rats, provision of extract was carried out starting the first day after the mother gave birth, and measurement were take on 6th, 11th, 16th to 21st day The volume of milk consumed by five rats was counted as difference of body weight after and before breastfeeding. Moringa nutritional assessment is carried out using standard methods on, iron, calcium and potassium content; vitamin A and vitamin C content and protein level. Fenugreek-moringa extract (1: 1) dose of 30 mg/200g bb, can increase the volume of breast milk of female rat larger than the untreated group (p≤0.05). The cumulative weight gain of rat infants was greater than the control group (p≤0.05), according to the nutrients obtained from the fenugreek-moringa extract. Moringa leaves as a component of extract, have high nutritional value of minerals, vitamins and proteins and have advantages as lactogogum. Conclusion: The administration of moringa leaf and fenugreek seed extract (1: 1) dose of 30 mg / 200 g bb in breastfeeding female rats has a 2.4 times greater potential in increasing milk expenditure compared to breastfeeding female rats who did not get the same extract. Fenugreek-moringa extract has superior potential as laktagogum and at the same time a high nutritional supplement. Abstrak Proporsi kejadian stunting pada anak usia di bawah 5 tahun (balita) di Indonesia tercatat sekitar 30,8% pada tahun 2018. Salah satu hal yang diduga berkaitan dengan terjadinya stunting adalah gizi kurang yang dapat dikaitkan dengan pemberian air susu ibu (ASI) eksklusif. Biji klabet memiliki khasiat sebagai laktagoga dan daun kelor selain mempunyai khasiat sebagai laktagoga, juga mempunyai keunggulan pada kandungan gizinya, terutama kandungan protein yang tinggi, golongan mineral dan vitamin. Penelitian bertujuan untuk menilai potensi formula ekstrak biji klabet (Trigonella foenum-graecum L.) dan daun kelor (Moringa oleifera Lamk.) sebagai suplemen pelancar pengeluaran air susu sekaligus memiliki kandungan nutrisi yang tinggi. Metode menggunakan test weighting method pada tikus betina hamil, dengan cara mengukur volume air susu yang diminum oleh bayi tikus. Dilakukan uji pada lima kelompok induk tikus yaitu: kelompok yang diberi ekstrak klabet dan kelor (1:1) dengan dosis I, II dan III yang berbeda; kelompok pembanding (moloco) dan kelompok kontrol (akuades). Tiap induk tikus menyusui lima ekor anak tikus. Pemberian bahan uji dilakukan mulai hari pertama setelah induk tikus melahirkan, dan pengukuran dilakukan pada hari ke-6, 11, 16 sampai 21. Volume air susu yang diminum lima ekor anak tikus dihitung sebagai selisih berat badan sesudah dan sebelum disusui induknya. Penilaian gizi kelor dilakukan menggunakan metoda standar, terhadap kandungan mineral besi, kalsium dan kalium; kandungan vitamin A dan vitamin C, serta kadar protein. Hasil penelitian menunjukkan ekstrak klabet-kelor (1:1) dosis 30 mg/200g bb, dapat memperbanyak pengeluaran volume air susu induk tikus lebih besar dari kelompok yang tidak diberi perlakuan (p≤0,05). Pertambahan berat kumulatif bayi tikus, lebih besar dibandingkan kelompok kontrol (p≤0,05), sesuai dengan gizi yang didapat dari ekstrak klabet-kelor. Daun kelor sebagai komponen ekstrak, mempunyai nilai gizi mineral, vitamin, dan protein yang tinggi serta memiliki keunggulan sebagai laktogogum. Kesimpulan penelitian adalah pemberian ekstrak biji klabet-daun kelor (1:1) dosis 30 mg/200g bb pada tikus betina menyusui mempunyai potensi 2,4 kali lebih besar dalam meningkatkan pengeluaran air susu dibandingkan kelompok tikus betina menyusui yang tidak mendapatkan ekstrak yang sama. Ekstrak biji klabet-kelor mempunyai potensi unggul sebagai laktagogum dan sekaligus suplemen dengan gizi tinggi.


2012 ◽  
Vol 6 (38) ◽  
pp. 5119-5127 ◽  
Author(s):  
Dinakaran Vasudevan ◽  
Sridharan Subhashree ◽  
Periyasamy Latha ◽  
Sudha Rani Sankaramoorthy

2011 ◽  
Vol 19 (1) ◽  
pp. 70-80 ◽  
Author(s):  
Saber A. Sakr ◽  
Salama M. El-shenawy ◽  
Ahmed M. Al-Shabka

2011 ◽  
Vol 61 (3) ◽  
pp. 303-317 ◽  
Author(s):  
Saber A. Sakr ◽  
Ezz M. El-Gamal

AbstractAdriamycin (ADR) is an anticancer drug used in treatment of a variety of neoplastic lesions. Its use is limited due to diverse toxicities including cardiotoxicity, hepatotoxicity and nephrotoxicity. The present work was conducted to study the effect of fenugreek seed extract on nephrotoxicity induced by ADR in albino rats. Administrating animals with a single dose of ADR (10 mg/kg body weight) induced histopathological, immunohistochemical and biochemical alterations. Kidneys of ADR-treated rats showed many histopathological alterations. The renal tubules were degenerated and the glomeruli were atrophied. The intertubular spaces were infiltrated by inflammatory leucocytic cells. An increase in expression of alfa-SMA was recorded. ADR caused marked elevation in serum creatinine and blood urea nitrogen. Moreover, ADR administration to animals significantly increased the concentration of malondialdehyde and decreased the activity of superoxide dismutase (SOD) in kidney tissues. These changes were time-dependent. Treating animals with ADR and aqueous seed extract of fenugreek (0.4 g/kg) led to an improvement in the histological structure of the kidney together with a decrease in expression of α-SMA. Urea and creatinine were significantly decreased. Moreover, fenugreek treatment reduced the concentration of malondialdehyde (lipid peroxidation marker) and increased the activity of antioxidant enzyme, SOD. In conclusion, the results of the present work indicated that fenugreek seeds had ameliorative effect on kidney damage induced by ADR and this may be mediated by its potent antioxidant effects


2015 ◽  
Vol 95 (4) ◽  
pp. 561-568 ◽  
Author(s):  
M. M. Hossain ◽  
M. Begum ◽  
C. M. Nyachoti ◽  
J. D. Hancock ◽  
I. H. Kim

Hossain, M. M., Begum, M., Nyachoti, C. M., Hancock, J. D. and Kim, I. H. 2015. Dietary fenugreek seed extract improves performance and reduces fecal E. coli counts and fecal gas emission in lactating sows and suckling piglets. Can. J. Anim. Sci. 95: 561–568. This study evaluated the efficacy of fenugreek seed extract (FSE) in sows and suckling piglets. The trial was conducted with 78 multiparous sows and their litters in a 28-d feeding trial, and one of three diets: CON (control, basal diet); FSE1 (basal diet+0.1% FSE), and FSE2 (basal diet+0.2% FSE). Piglets suckling sows fed the FSE1 and FSE2 diets had higher average daily gain compared with those suckling sows fed the CON from week 2 to weaning (linear, P<0.05). At weaning, sows fed the FSE1 and FSE2 diets had higher immunoglobulin G and lower low-density lipoprotein–cholesterol concentrations compared with those fed the CON diet (linear, P<0.05). Sows fed the FSE2 diet linearly reduced total cholesterol concentrations at weaning (P<0.05). Piglets suckling sows fed the FSE2 diet had higher immunoglobulin G and red blood cell concentrations compared with those suckling sows fed the CON diet (linear, P<0.05). Sows fed the FSE2 diet had higher apparent total tract digestibility of N and gross energy and reduced fecal E. coli counts and ammonia gas emission compared with those fed the CON diet at weaning (linear, P<0.05). In conclusion, results indicate that feeding sows a diet supplemented with FSE in late gestation and lactation improved performance in sows and suckling piglets.


Author(s):  
Madhulika Singh ◽  
G. N. Verma

Aims: The present study was undertaken to assess the antispermatogenic and antifertility efficacy of ethanolic extract of Trigonella foenum-greacum seeds. Methodology: Aqueous solution of the extract (250 mg/kg b.wt/day) when administered orally for 45 days to adult male albino rat (Duckray strain). Results: Seeds extract caused inhibition of spermatogenesis as well as inability to mate with normal untreated female rats of proven fertility. There occurred a significant decrease in absolute and relative weights of testis and seminal vesicle and increase in epididymal weight, whereas of ventral prostate and coagulating gland remained unchanged. Sperm concentration and motility in the cauda epididymis was also decreased. Increased incidence of separation of head and tail pieces of spermatozoa was seen in the epididymal smear. Histologically, testis of experimental animals showed arrest of spermatogenesis at the secondary spermatocytes stage and there was deposition of cellular debris in semineferous tubular lumen. The spermatids were not properly developed and interstitial cells were very sparse and degenerated. However, there was no evidence of damage to the spermatogonia. Oral treatment of extract did not affect the body weight of animals. Quantitative estimation of marker testicular enzymes, e.g., sorbitol dehydrogenase showed a significant decrease whereas lactate dehydrogenase and gamma-glutamyl transpeptidase levels were significantly increased. In conclusion: These results suggest that T. foenum-greacum, may induce male infertility in rats, therefore, should be considered further as a potential male antifertility agent.


2021 ◽  
Vol 9 (3) ◽  
pp. 353-361
Author(s):  
Revathi C ◽  
◽  
Vadivelu J ◽  

Physalis peruviana has been widely used as a medicinal herb for treating various diseases since ancient times. This study aimed to examine the hepatoprotective potential of the ethanolic extract of P. peruviana fruit (EPPF) against lead acetate (LA) intoxicated male albino rats. The experimental rats were divided into ten groups of 4 animals. Animal of Group I served as normal, Group II animals were administered orally 15 mg/Kg body weight of LA, Group III to V animals received EPPF 150mg/kg, 300 mg/kg and 600 mg/kg respectively, Group VI animals received standard silymarin 50 mg/kg, and Group VII to X were treated with LA (15 mg/kg) with EPPF 150, 300, 600 and std 50 mg/kg, orally for 32 days respectively. The degree of protection was measured by estimating hematological parameters such as Hb, RBC, WBC, PCV, platelets, MCV, MCHC, and ESR and biochemical parameters such as urea, creatinine, Cholesterol, HDL, LDL, VLDL, bilirubin, SGOT, SGBT, ALP, GGT, protein, albumin and globulin. Lead acetate induced alterations of hematological and biochemical parameters were observed in group II animals and these levels brought back to normal in the animals treated with EPPF at the concentrations of 150, 300 and 600 mg/kg which was significantly similar to silymarin treated animals. The data of the results obtained depicted that the fruit extract of P. peruviana was found to have promising protective efficacy against lead acetate induced liver toxicity.


1993 ◽  
Vol 7 (2) ◽  
pp. 134-138 ◽  
Author(s):  
R. Kamal ◽  
R. Yadav ◽  
J. D. Sharma

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