scholarly journals Study of the Association Between Diabetes and Helicobacter Pylori Infection in a Tunisian Population

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Chaima Jemai ◽  
Rim Rachdi ◽  
Sonia Bellamine ◽  
Lamia Bouallegue ◽  
Faika Ben Mami
Keyword(s):  
Helicobacter Pylori ◽  
Medical Records ◽  
Tunisian Population ◽  
Control Group ◽  
Patients With Diabetes ◽  
And Gender ◽  
H Pylori ◽  
Control Study

Introduction: The association between diabetes and Helicobacter Pylori (H. Pylori) infection remains controversial in the literature. The aim of our study was to search an association between diabetes and H. Pylori infection. Methods: This is a case-control study carried out in 2017 over 3 months (September-October-November), collecting 120 patients with dyspepsia, matched for age and gender into two groups: a group of 77 patients with diabetes, and a group of control cases made of 43 non-diabetics. Diabetes was defined according to the American Diabetes Association (ADA) of 2017. Clinical, biological, endoscopic and anatomopathological data were collected from medical records. Results: The average age of the patients was 50±2,1 year. The sex ratio was 0.51. 34.2% (n=41) patients were male. Diabetes was type 2 in the majority of cases (88.31%) and type 1 in 11.68% only. H. Pylori infection was more prevalent in diabetics (19.48%, 11.63%, p=0.27). H. Pylori infection was more prevalent in type 1 diabetics (44.44%, 16.18%, p= 0.04). The frequency of upper endoscopic lesions in diabetics and controls was 70.13% and 74.42%, respectively. Chronic gastritis, gastric atrophy, and intestinal metaplasia were found in 61%, 3.9% and 2.6% of the cases in the group of diabetics and 62.79%, 6.98% and 4.65% respectively in the control group (p= not significant (NS)). Conclusion: Our study shows the absence of a significant association between diabetes and H. Pylori infection, as well as the absence of endoscopic and histological specificities of this infection in patients with diabetes.

The evaluation of nasal mucociliary clearance in patients with Helicobacter pylori infection

2019 ◽  
Vol 133 (03) ◽  
pp. 220-223
Author(s):  
S Üstün Bezgin ◽  
T Çakabay ◽  
K Irak ◽  
M Koçyiğit ◽  
B Serin Keskineğe ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Mucociliary Clearance ◽  
Endoscopic Examination ◽  
Control Group ◽  
Clearance Time ◽  
Saccharine Test ◽  
Significant Difference ◽  
And Gender ◽  
H Pylori ◽  
Nasal Mucociliary Clearance

AbstractObjectiveThis study aimed to examine nasal mucociliary clearance time in patients with Helicobacter pylori infection.MethodsFifty patients who were newly diagnosed with H pylori infection using gastric biopsy in the gastroenterology out-patient clinic, and 50 age- and gender-matched healthy adults who were admitted to the otorhinolaryngology out-patient clinic, were included in this study. After an otorhinolaryngological examination (anterior rhinoscopy and nasal endoscopic examination), the nasal mucociliary clearance time of each subject was calculated using the saccharine test.ResultsThe mean mucociliary clearance time was 06:29 ± 3:31 minutes (range, 00:55–15:19 minutes) in the control group and 10:12 ± 06:09 minutes (range, 01:28–32:00 minutes) in the study group. Comparisons of the two groups revealed a statistically significant difference (p = 0.002).ConclusionNasal mucociliary clearance time was significantly increased in patients with H pylori infection. The results suggest that H pylori infection may have an unfavourable effect on nasal mucociliary clearance.

scholarly journals Novel Functions for Glycosyltransferases Jhp0562 and GalT in Lewis Antigen Synthesis and Variation in Helicobacter pylori

2012 ◽  
Vol 80 (4) ◽  
pp. 1593-1605 ◽  
Author(s):  
Mary Ann Pohl ◽  
Sabine Kienesberger ◽  
Martin J. Blaser
Keyword(s):  
Helicobacter Pylori ◽  
Wild Type ◽  
Sequence Analyses ◽  
Upstream Gene ◽  
Content Type ◽  
Lewis Antigen ◽  
Mutant Strains ◽  
H Pylori

ABSTRACTLewis (Le) antigens are fucosylated oligosaccharides present in theHelicobacter pylorilipopolysaccharide. Expression of these antigens is believed to be important forH. pyloricolonization, since Le antigens also are expressed on the gastric epithelia in humans. A galactosyltransferase encoded by β-(1,3)galTis essential for production of type 1 (Leaand Leb) antigens. The upstream genejhp0562, which is present in many but not allH. pyloristrains, is homologous to β-(1,3)galTbut is of unknown function. BecauseH. pyloridemonstrates extensive intragenomic recombination, we hypothesized that these two genes could undergo DNA rearrangement. A PCR screen and subsequent sequence analyses revealed that the two genes can recombine at both the 5′ and 3′ ends. Chimeric β-(1,3)galT-like alleles can restore function in a β-(1,3)galTnull mutant, but neither native nor recombinantjhp0562can. Mutagenesis ofjhp0562revealed that it is essential for synthesis of both type 1 and type 2 Le antigens. Transcriptional analyses of both loci showed β-(1,3)galTexpression in all wild-type (WT) and mutant strains tested, whereasjhp0562was not expressed injhp0562null mutants, as expected. Sincejhp0562unexpectedly displayed functions in both type 1 and type 2 Le synthesis, we asked whethergalT, part of the type 2 synthesis pathway, had analogous functions in type 1 synthesis. Mutagenesis and complementation analysis confirmed thatgalTis essential for Lebproduction. In total, these results demonstrate thatgalTandjhp0562have functions that cross the expected Le synthesis pathways and thatjhp0562provides a substrate for intragenomic recombination to generate diverse Le synthesis enzymes.

scholarly journals Helicobacter pylori–binding nonacid glycosphingolipids in the human stomach

2018 ◽  
Vol 293 (44) ◽  
pp. 17248-17266 ◽  
Author(s):  
Chunsheng Jin ◽  
Angela Barone ◽  
Thomas Borén ◽  
Susann Teneberg
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Structural Complexity ◽  
Human Stomach ◽  
Carbohydrate Binding ◽  
Human Gastric Mucosa ◽  
H Pylori ◽  
Contrast Information

Helicobacter pylori has a number of well-characterized carbohydrate-binding adhesins (BabA, SabA, and LabA) that promote adhesion to the gastric mucosa. In contrast, information on the glycoconjugates present in the human stomach remains unavailable. Here, we used MS and binding of carbohydrate-recognizing ligands to characterize the glycosphingolipids of three human stomachs from individuals with different blood group phenotypes (O(Rh−)P, A(Rh+)P, and A(Rh+)p), focusing on compounds recognized by H. pylori. We observed a high degree of structural complexity, and the composition of glycosphingolipids differed among individuals with different blood groups. The type 2 chain was the dominating core chain of the complex glycosphingolipids in the human stomach, in contrast to the complex glycosphingolipids in the human small intestine, which have mainly a type 1 core. H. pylori did not bind to the O(Rh−)P stomach glycosphingolipids, whose major complex glycosphingolipids were neolactotetraosylceramide, the Lex, Lea, and H type 2 pentaosylceramides, and the Ley hexaosylceramide. Several H. pylori-binding compounds were present among the A(Rh+)P and A(Rh+)p stomach glycosphingolipids. Ligands for BabA-mediated binding of H. pylori were the Leb hexaosylceramide, the H type 1 pentaosylceramide, and the A type 1/ALeb heptaosylceramide. Additional H. pylori-binding glycosphingolipids recognized by BabA-deficient strains were lactosylceramide, lactotetraosylceramide, the x2 pentaosylceramide, and neolactohexaosylceramide. Our characterization of human gastric receptors required for H. pylori adhesion provides a basis for the development of specific compounds that inhibit the binding of this bacterium to the human gastric mucosa.

scholarly journals Helicobacter pylori infection among type 2 diabetics: a case control study

2020 ◽  
Vol 8 (3) ◽  
pp. 1047
Author(s):  
Hafiz Qazi Muhammad Amir ◽  
Omaila Ikram ◽  
Muhammad Tayyab Zia ◽  
Fareha Kashan Theba ◽  
Naila Ikram ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Case Control Study ◽  
Case Control ◽  
Helicobacter Pylori Infection ◽  
P Value ◽  
Type 2 Diabetics ◽  
Dyspeptic Symptoms ◽  
H Pylori ◽  
Control Study

Background: Helicobacter pylori infection has been associated with hyperglycemia among type 2 diabetics. The objective of this study was to compare the H. pylori infection frequency in diabetic and non-diabetic patients.Methods: This case-control study was done at Al-Tibri Medical College and Hospital from May 2019 to August 2019. After written and informed consent, patients between 18-75 years with epigastric burning, dyspepsia, regurgitation were included and with history of eradication therapy, antibiotic or NSAID use in the last 6 months or surgery of upper GI tract months were excluded. Type 2 diabetics were placed in one group and non-diabetic individuals in another. Both groups were compared for presence of H. pylori infection. Data was analysed using SPSS. Demographic variables included age, gender and status of H. pylori infection. Quantitative data was expressed as frequency and percentages. Chi-square test was applied to test for significance keeping p-value of <0.05 statistically significant.Results: From 480 patients, 355 patients showed positive H. pylori, among them 282 were diabetic and 73 non-diabetic (p-value <0.001). Amongst the 355 diabetics, 55% were male Among 73 non-diabetics, 64% were male. All the patients in the study had dyspeptic symptoms and complained of dyspepsia, epigastric burning and regurgitation.Conclusions: A substantial relationship between H. pylori infection among type 2 diabetes mellitus patients was observed compared to non-diabetics. As a result, diabetic patients having active dyspeptic symptoms should undergo further confirmatory tests for diagnosing H. pylori infection.

scholarly journals Predictors of Early Retinal Changes in Diabetes Mellitus

2020 ◽  
Vol 17 (1) ◽  
pp. 88-95
Author(s):  
V. S. Kulybysheva ◽  
I. A. Ronzina ◽  
A. A. Gamidov ◽  
V. G. Motalov ◽  
V. N. Nikolenko
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dynamic Control ◽  
Clinical Manifestations ◽  
Light Sensitivity ◽  
Control Group ◽  
Patients With Diabetes ◽  
Almost All

Purpose. Determining the functional state of the outer and inner retina’s layers in patients with diabetes mellitus (DM) type 1 and 2 before the clinical manifestations and in the early stages of diabetic retinopathy (DR) using the methods of multifocal electroretinography (mfERG) and microperimetry (MP).Patients and methods. 91 patients were examined (182 eyes). The patients were divided into 4 groups: 1st — 23 people (46 eyes) with diabetes without DR (the duration of the disease is up to 2 years); 2nd — 22 people (44 eyes) with diabetes without DR (diabetes from 2 to 8 years); 3rd — 24 people (48 eyes) with NPDR on the background of diabetes; 4th — 22 people (44 eyes) of the control group (healthy eyes). In addition to the standard ophthalmologic examination, all patients were registered mfERG (FOK1) on the diagnostic equipment EP-1000 Multifocal (Tomey, Germany) and carried out MP using the device “MAIA” (CenterVue, Italy).Results. According to mfERG, it has been established that the components of mfERG, the biopotential density and the amplitude of P1, are most sensitive to diabetic changes. In groups with type 1 and type 2 diabetes, there is a significant decrease in the density of P1 in comparison with the control group (p < 0.005, Mann-Whitney test), as well as a decrease in the amplitude of P1 on almost all tested rings (p < 0.005). In all groups, there is an increase in the latency of P1 in the central ring (0–2.3°). According to MP data, it was found that patients with type 1 and type 2 diabetes showed a decrease in the average light sensitivity in comparison with the control group, however, our data are within the reference values, regardless of the presence or absence of clinical manifestations of PD.Conclusion. As a result of the study, early functional and morphological disorders of the neurosensory apparatus of the eye in diabetes were identified. It is proved that mfER and MP allow to detect violations at the preclinical stage of DR and are necessary studies for the dynamic control of the progression of DR.

Đánh giá giá trị của nội soi NBI trong chẩn đoán nhiễm khuẩn Helicobacter pylori ở bệnh nhân viêm dạ dày mạn tính

2021 ◽  
Vol 16 (8) ◽  
Author(s):  
Thai Doan Ky ◽  
Hoang Kim Ngan ◽  
Pham Minh Ngoc Quang ◽  
Luu Tuan Thanh
Keyword(s):  
Helicobacter Pylori ◽  
Urease Test ◽  
H Pylori ◽  
Type 3

Mục tiêu: Đánh giá giá trị của nội soi dải tần hẹp (NBI) trong chẩn đoán nhiễm Helicobacter pylori (H. pylori). Đối tượng và phương pháp: Mô tả cắt ngang, hình ảnh nội soi NBI được phân loại thành 4 type dựa trên cấu trúc khe tuyến và mạch máu dưới biểu mô. Bệnh nhân đượclàm urease test và mô bệnh học để đối chiếu, qua đó đánh giá giá trị của nội soi NBI. Kết quả: 89 bệnh nhân được đưa vào nghiên cứu từ ngày 01/11/2020 đến ngày 30/05/2021, trong đó 32 bệnh nhân được xếp loại type 1 chiếm 36%, 34 bệnh nhân xếp loại type 2 (38,2%), 21 bệnh nhân xếp loại type 3 (23,6%) và 2 bệnh nhân được xếp loại type 4 (2,2%), 36 bệnh chẩn đoán dương tính chiếm 53,7%, 31 bệnh nhân âm tính chiếm 46,3%. Độ nhạy của type 2 + type 3 + type 4 trong chẩn đoán H. pylori là 97,22%, độ đặc hiệu là 77,42%, giá trị dự báo dương tính là 83,33%, giá trị dự báo âm tính là 96%. Kết luận: Nội soi NBI có giá trị cao trong chẩn đoán nhiễm H. pylori.

scholarly journals Helicobacter pylori outer membrane protein and virulence marker differences in expatriate patients

2016 ◽  
Vol 144 (10) ◽  
pp. 2200-2208 ◽  
Author(s):  
J. YAKOOB ◽  
Z. ABBAS ◽  
W. JAFRI ◽  
R. KHAN ◽  
S A. SALIM ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Virulence Marker ◽  
Virulence Markers ◽  
Local Residents ◽  
H Pylori

SUMMARYWe studied the prevalence of Helicobacter pylori virulence markers, e.g. cytotoxin associated gene (cagA), cagA promoter, vacuolating associated cytotoxin A (vacA) alleles induced by contact with epithelium (iceA type), and outer membrane protein Q (hopQ) in expatriates and compared them with those in local residents. Gastric biopsies were obtained at endoscopy for culture, histology and PCR for virulence marker and hopQ. Of 309 patients, 236 (76%) were males with a mean age of 45 years. A total of 102 patients were expatriates. hopQ type 1 was present in 98 (47%) local residents compared to 88 (86%) expatriates (P < 0·001), while hopQ type 2 was present in 176 (85%) local residents, compared to 60 (59%) expatriates (P < 0·001). H. pylori virulence marker cagA was positive in 97 (47%) local residents compared to 86 (84%) expatriates (P < 0·001) while cagA-P was positive in 72 (35%) local residents compared to 87 (85%) expatriates (P < 0·001). iceA type 1 was positive in 157 (76%) local residents compared to 45 (44%) expatriates (P < 0·001), while iceA type 2 was positive in 81 (39%) local residents compared to 86 (84%) expatriates (P < 0·001). Distribution of H. pylori cagA, cagA promoter, iceA and hopQ type in local residents and expatriates was different. H. pylori virulence markers were associated with severe pathology in expatriates.

scholarly journals Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide

2008 ◽  
Vol 57 (5) ◽  
pp. 585-591 ◽  
Author(s):  
Eleonora Altman ◽  
Heriberto Fernández ◽  
Vandana Chandan ◽  
Blair A. Harrison ◽  
Myra Wilson Schuster ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Blood Group ◽  
Western Blotting ◽  
Gel Electrophoresis ◽  
Indirect Elisa ◽  
Antigen Expression ◽  
Whole Cell ◽  
H Pylori

Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Lex) and Ley epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Lea and Leb structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Lex and/or Ley epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Leb blood group determinants, in addition to type 2 Lex and Ley structures. Six strains (16 %) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3 %).

scholarly journals Effect of Gender and Age on the Correlation between Helicobacter pylori and Colorectal Adenomatous Polyps in a Chinese Urban Population: A Single Center Study

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Xia-Xia Zhao ◽  
Ming-Hao Liu ◽  
Rui-Ling Wang ◽  
Tian Tian
Keyword(s):  
Helicobacter Pylori ◽  
Size Distribution ◽  
Control Group ◽  
Age And Gender ◽  
Gender And Age ◽  
Single Center Study ◽  
Healthy Control ◽  
And Gender ◽  
H Pylori ◽  
Beijing China

Objectives. To investigate whether Helicobacter pylori (H. pylori) infection increases the risk of colorectal adenomatous polyp (CAP) in the context of age and gender. Methods. A total of 563 study subjects (male/female, 368/195) from Beijing, China, with higher nursing level who underwent colonoscopy were retrospectively collected. H. pylori and CAP were detected by carbon-13 urea breath test and colorectal colonoscopy. The correlations between the number, size, distribution, and pathological grade of CAP and H. pylori infection were analyzed. The population was further stratified by age and gender in order to examine the risk of H. pylori and CAP in the context of these variables. The influence of H. pylori on the risk of CAP was assessed by logistic regression model. Results. 315 participants were diagnosed with CAP, and 207 participants were classified as healthy controls. The prevalence of H. pylori in the CAP group was significantly higher than that in the healthy control group (119/315, 37.8% versus 44/207, 21.3%) (p<0.001). The proportion of H. pylori positive plus CAP in participants <50 years old was significantly higher than that in participants >50 years old (87/250; 34.8% versus 32/65; 49.2%) (p=0.033). Furthermore, H. pylori infection was identified as one of the major risk factors of CAP (OR=2.679; 95% CI: 1.717-4.179, p<0.001), independent of age, sex, and body mass index and was correlated with size, distribution, and pathological grading of CAP (p<0.001). Conclusions. H. pylori is a major risk factor for CAP. Further studies are needed to assess the effects of H. pylori treatment or persistent infection on the occurrence or recurrence of CAP.

scholarly journals Rhesus monkey gastric mucins: oligomeric structure, glycoforms and Helicobacter pylori binding1

2004 ◽  
Vol 379 (3) ◽  
pp. 765-775 ◽  
Author(s):  
Sara LINDÉN ◽  
Thomas BORÉN ◽  
André DUBOIS ◽  
Ingemar CARLSTEDT
Keyword(s):  
Helicobacter Pylori ◽  
Rhesus Monkey ◽  
Blood Group ◽  
T Antigen ◽  
Blood Group Antigens ◽  
Human Beings ◽  
Sialyl Lex ◽  
H Pylori

Mucins isolated from the stomach of Rhesus monkey are oligomeric glycoproteins with a similar mass, density, glycoform profile and tissue localization as human MUC5AC and MUC6. Antibodies raised against the human mucins recognize those from monkey, which thus appear to be orthologous to those from human beings. Rhesus monkey muc5ac and muc6 are produced by the gastric-surface epithelium and glands respectively, and occur as three distinct glycoforms. The mucins are substituted with the histo blood-group antigens B, Lea (Lewis a), Leb, Lex, Ley, H-type-2, the Tn-antigen, the T-antigen, the sialyl-Lex and sialyl-Lea structures, and the expression of these determinants varies between individuals. At neutral pH, Helicobacter pylori strains expressing BabA (blood-group antigen-binding adhesin) bind Rhesus monkey gastric mucins via the Leb or H-type-1 structures, apparently on muc5ac, as well as on a smaller putative mucin, and binding is inhibited by Leb or H-type-1 conjugates. A SabA (sialic acid-binding adhesin)-positive H. pylori mutant binds to sialyl-Lex-positive mucins to a smaller extent compared with the BabA-positive strains. At acidic pH, the microbe binds to mucins substituted by sialylated structures such as sialyl-Lex and sialylated type-2 core, and this binding is inhibited by DNA and dextran sulphate. Thus mucin–H. pylori binding occurs via at least three different mechanisms: (1) BabA-dependent binding to Leb and related structures, (2) SabA-dependent binding to sialyl-Lex and (3) binding through a charge-mediated mechanism to sialylated structures at low pH values.

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