scholarly journals Antibacterial activity test of Simargaolgaol (Aglaonema modestum Schott ex Engl) leaves extract against Escherichia coli and Salmonella typhi bacteria

2021 ◽  
Vol 13 (2) ◽  
pp. 151-158
Author(s):  
Tiurma Solomasi Zega ◽  
◽  
Putri Mandaoni Pakpahan ◽  
Rahmayani Siregar ◽  
Givinda Sitompul ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Activity Test ◽  
Inhibitory Power ◽  
Ethanol Extracts

The Simargaolgaol (Aglaonema modestum Schott ex Engl) plant is a plant that grows wild in Barus District, Central Tapanuli, North Sumatra which is used by the community as a medicinal plant. This study aims to determine the antibacterial activity of n-hexane, ethyl acetate and ethanol extracts from Simargaolgaol leaves against Escherichia coli and Salmonella typhi bacteria. The antibacterial activity test of Simargaolgaol leaves was carried out using the disc diffusion method. The results of antibacterial activity showed that the highest inhibitory power of Simargaolgaol leaf extract was ethanol extract (polar), ethyl acetate extract (semi polar) and n-hexane extract (non polar). The inhibitory power of ethanol extract, ethyl acetate, and n-hexane against Escherichia coli bacteria was 13.1 mm (strong); 9.7 mm (medium); 8.0 mm (medium) and for Salmonella typhi bacteria respectively 11.2 mm (strong); 10.7 mm (strong) and 9.3 mm (medium). With the concentration of the extract in the diameter is 10%. Based on this, it can be concluded that Simargaolgaol leaf extract has potential as an antibacterial. Keywords: Aglaonema modestum Schott ex Engl, Antibacterial activity, Escherichia coli, Salmonella typhi

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK n-HEKSAN, ETIL ASETAT DAN ETANOL KULIT BATANG KAYU EBONI (Diospyros celebica Bakh.) MENGGUNAKAN METODE DIFUSI

KOVALEN ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 233-239
Author(s):  
Ni Luh Sumitriasih ◽  
Ahmad Ridhay ◽  
Indriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Tree Bark ◽  
Diffusion Method ◽  
Well Test ◽  
Activity Test ◽  
Ethanol Extracts

A study was conducted on the antibacterial activity test on n-hexane, ethyl acetate, and ethanol extracts of ebony bark (Diospyros celebica Bakh.) using a diffusion method. The purpose of this research is to know the antibacterial activity of ebony bark against Staphylococcus aureus and Escherichia coli bacteria using n-hexane, ethyl acetate dan ethanol. The method of extraction used in this research was maceration method with three polarity levels of solvent. The antibacterial activity test was performed by diffusion-well test. The results showed that ethyl acetate extract gave the highest inhibition of 26.14 mm in gram-positive bacteria (Staphylococcus aureus) and ethanol extract of 25.97 mm in gram-negative bacteria (Escherichia coli). Keywords: Ebony (Diospyros celebica. Bakh), Tree Bark, Inhibitory, Antibacterial

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DADAP SEREP (Erythrina lithosperma Miq.) TERHADAP PERTUMBUHAN Escherichia coli

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Asep Abdul Rahman ◽  
Ridwan Firmansyah ◽  
Lulu Setyabudi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Abdominal Pain ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Activity Test ◽  
Paper Disc

Dadap Serep (Erythrina lithosperma Miq.) is one of the plants containing secondary metabolites in the form of alkaloids, flavonoids and tannins. Empirically dadap serep can be used to treat fever, abdominal pain, breast milk, prevent miscarriage, inflammation and cough. The aim of this research is to know the activity of ethanol extract in dadap serep (Erythrina lithosperma Miq.) in inhibiting growth of Escherichia coli ATCC 8939. The dadap serep leaves were extracted by maceration method using 96% ethanol solvent. The leaf thickness extract of dadap serep with concentration of 20%, 40%, 60% and 80% tested its antibacterial activity against growth of Escherichia coli ATCC 8939 using paper disc diffusion method. The result of concentration of leaf extract of dadap serep, obtained rendemen as much as 23,53%. Then the result of antibacterial activity test showed that leaf extract of dadap serep with concentration of 20% had no inhibition to growth of Escherichia coli ATCC 8939, while at concentration extract 40%, 60%, and 80% had inhibitory to growth of Escherichia coli ATCC 8939 with category weak resistor response.Keywords: Dadap serep (Erythrina lithosperma Miq.), Antibacterial activity, Escherichia coli ATCC 8939

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

scholarly journals The Inhibitory Power Extract Of Balsamica Plant (Impatiens balsamica L) on Aeromonas hydrophila Bacteria Growth

2014 ◽  
Vol 2 (2) ◽  
pp. 40
Author(s):  
Galih Arif Kusuma ◽  
Sammy N. J. Longdong ◽  
Reiny A. Tumbol
Keyword(s):  
Antibacterial Activity ◽  
Aeromonas Hydrophila ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Bacteria Growth ◽  
Activity Test ◽  
Inhibitory Power

This research aimed to assess the potential use of extracts of leaves, flowers, and stems of the balsamica plant increase inhibitory effect on the growth of Aeromonas hydrophila. The content of the plant contains a compound balsamica naphthoquinone, coumarin derivatives, tannins, flavonoids, and steroids. The active compounds have the ability as an antimicrobial. A hidrophyla bacteria, including opportunistic pathogens are almost always found in water and often cause disease when the fish in adverse conditions. Antibacterial activity test in this research using the spread plate method. Data obtained in the form of the extract, the results of bacterial inoculation, and the inhibition test results, will be analyzed, displayed with pictures and described descriptively. The result of the process of maceration extraction using ethanol 70% was obtained three extracts are concentrated leaf extract: 28.75 g, flower: 12.82 g, and rods: 29.48 g. The result of antibacterial extracts of leaves, stems, and flowers balsamica plant showed inhibitory activity on the A hydrophila bacteria that seems to be indicated by a clear zone around the paper disc. Based on the classification of leaf extract and extract of the stem can be classified in the class of strong, because the inhibition zone formed by the leaf extract is equal to 11.2 mm, and extract stem with 13.7 mm inhibition zone. Ability flower extract in inhibiting the growth of A hidrophila bacteria with 21.4 mm inhibition zone showed that the extract was included in a very strong class. Keywords: The ethanol extract balsamica, Impatiens balsamica L, Aeromonas hydrophila, antibacterial activity test.

scholarly journals SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Vera Nurviana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Water Fraction ◽  
Tetracycline Hcl

<p class="Default"><em>Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl.<br /> <br /> <strong></strong></em></p><p class="Default"><strong><em>Keywords: </em></strong><em>Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.</em></p>

scholarly journals Antibacterial Activity of Liverworts of Dumortiera hirsute (Sw.) Nees Ethyl Acetate Extract Against Pathogenic Bacteria

2021 ◽  
Vol 9 (2) ◽  
pp. 75
Author(s):  
Luthfiah Luthfiah ◽  
Dwi Setyati ◽  
Sattya Arimurti
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Well Diffusion Method

Dumortiera hirsuta is one of the liverworts that can be used as a medicinal to prevent infection by pathogenic bacteria. The content of secondary metabolites of D. hirsuta has potential as antibacterial properties includes flavonoids, alkaloids and steroids. This research is to analyze the antibacterial activity of moss D. hirsuta against pathogenic bacteria that will be beneficial to humans. Liverworts of D. hirsuta were extracted using ethyl acetate solvent and tested against three types of pathogenic bacteria using the agar well-diffusion method. The results of this study indicated that the ethyl acetate extract of D. hirsuta at 100% concentration could inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria. The range of antibacterial activity categories of the ethyl acetate extract of D. hirsuta to E. coli, S. aureus, and S. typhi between weak to moderate.

scholarly journals Antibacterial Activity of Solanum surattense Burm. F

1970 ◽  
Vol 6 (1) ◽  
pp. 1-4 ◽  
Author(s):  
E Sheeba
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Salmonella Typhi ◽  
Shigella Dysenteriae ◽  
Leaf Extracts ◽  
Solanum Surattense ◽  
Engineering And Technology ◽  
Ethanol Extracts

Ethanol extracts of Solanum surattense used in traditional medicine for treatmentof various infections. The antibacterial activity detected was againstStaphylococcus aureus, Streptococcus sp.; Bacillus subtilis, Escherichia coli,Pseudomonas aeruginosa, Salmonella typhi, Shigella dysenteriae and Vibriocholerae. The highest antibacterial activity was observed in 500μg concentrationof leaf extracts of all bacteria screened except Shigella dysenteriae. The minimumzone of inhibition observed in 25μg concentration of leaf extract exceptPseudomonas aeruginosa and Shigella dysenteriae. These results indicate that theextracts were bacteriostatic at higher concentrations.Keywords: Antibacterial activity; Solanum surattense; pathogenicmicro organisms.DOI: 10.3126/kuset.v6i1.3278Kathmandu University Journal of Science, Engineering and Technology Vol.6(1) 2010, pp 1-4

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine americana Merr) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella typhi

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 525
Author(s):  
Rafi’a Adinda Putri ◽  
Herny E.I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Paper Disk ◽  
Eleutherine Americana

ABSTRACTDayak onions (Eleutherine americana Merr) has been used as a traditional plant which has  benefits  for  many  diseases,  one  of  them  is  antibacterial. This study aims to determine the growth inhibition of ethanol extract of Dayak onions leaves on the bacterium S. aureus, E. coli and  S. typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby-Bauer diffusion). The results showed that ethanol extract of bawang dayak has the potential as an antibacterial and has antibacterial strength against S. aureus, E. coli and S. typhi. At concentrations of 20%, 40% and 60% forming inhibition zone diameters (16.23 mm, 19.18 mm, 21.25 mm) S. aureus, (10.7 mm, 13.98 mm, 15.87 mm) E. coli, and  (14.43 mm, 14.6 mm, 17.2 mm) S. typhi. At concentration of 20%, 40% and 60% included strong groups to inhibit E. coli and  S. typhi, while at concentration of 60%, it is included in a very strong group to inhibit the S. aureus bacteria. Keywords: Antibacterial activity, Bawang Dayak , Kirby-Bauer diffusion method   ABSTRAK Bawang Dayak (Eleutherine americana Merr) telah digunakan sebagai tanaman tradisional yang bermanfaat bagi banyak penyakit salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ekstrak etanol bawang dayak terhadap bakteri S. aureus, E.coli dan S. typhi menggunakan tiga kosentrasi yaitu 20%, 40% dan 60%. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan  menggunkan metode difusi dengan cakram kertas (difusi Kirby-Bauer). Hasil penelitian menunjukkan bahwa ekstrak etanol bawang dayak berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap S. aureus, E. coli dan S. typhi. Pada konsentrasi 20%, 40% dan 60% membentuk diameter zona hambat (16.23 mm, 19.18 mm, 21.25 mm) pada S. aureus, (10.7 mm, 13.98 mm, 15,87 mm) pada E. coli, (14.43 mm, 14.6 mm, 17.2 mm) pada S. typhi. Pada konsentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri E. coli dan S. typhi, sedangkan pada konsentrasi 60% termasuk dalam golongan sangat kuat untuk menghambat bakteri S. aureus. Kata kunci: Aktivitas antibakteri, Bawang Dayak, metode difusi Kirby-Bauer

scholarly journals Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 42
Author(s):  
Florentin Natalia Melsadalam ◽  
Dewa Gede Katja ◽  
Meiske S. Sangi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder  dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri  dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah  estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri  Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

scholarly journals UJI DAYA HAMBAT EKSTRAK ETANOL DAUN KACAPIRING (Gardenia augusta) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella thypi

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 268
Author(s):  
Serly D. S. Toding ◽  
Herny E. I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Inhibitory Power ◽  
Paper Disc

ABSTRACT One of the potential medicinal plants as traditional medicine is gardenia plant (Gardenia augusta). Gardenia leaf extract contains alkaloid compounds, flavonoids, tannins, saponins and triterpenoids, which are known to have antibacterial abilities..The aim of this research was to determine the antibacterial activity of gardenia leaf extract on the growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi. The examination on the inhibitory power using the Kirby-Bauer method. The Kirby-Bauer method is referring to the diffusion method using paper disc with five treatments, namely the extract of gardenia leaves with a substance concentration of 20%, 40%, and 60% as well positive control (Ciprofloxacin) and negative control (aquades). Based on the research results obtained, it can be concluded that gardenia leaf extract can inhibit growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi at a concentration of 20%, 40% and 60% and classified as strong and medium category. Keywords  :    Gardenia augusta, antibacterial, of Staphylococcus aureus, Escherichia coli, Salmonella thypiABSTRAK  Salah satu tanaman obat dengan potensi sebagai obat tradisional adalah tanaman kacapiring (Garddenia augusta). Daun kacapiring mengandung senyawa alkaloid, flavonoid, tanin, saponin dan triterpenoid, yang berpotensi sebagai antibakteri sehingga digunakan dalam penelitian ini.Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kacapiring terhadap pertumbuhan Staphylococcus aureus, Escherichia coli dan Salmonella thypi. Pengujian daya hambat menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas dengan 5 perlakuan yaitu ekstrak daun kacapiring dengan konsentrasi 20%, 40%, dan 60% serta kontrol positif (ciprofloxacin) dan kontrol negatif (akuades). Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak daun kacapiring dapat menghambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Salmonella thypi pada konsentrasi 20%, 40% dan 60% dan tergolong dalam kategori kuat dan sedang. Kata Kunci  : Kacapiring, Antibakteri, Staphylococcus aureus, Escherichia coli, Salmonella thypi

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