scholarly journals The Inhibitory Power Extract Of Balsamica Plant (Impatiens balsamica L) on Aeromonas hydrophila Bacteria Growth

2014 ◽  
Vol 2 (2) ◽  
pp. 40
Author(s):  
Galih Arif Kusuma ◽  
Sammy N. J. Longdong ◽  
Reiny A. Tumbol
Keyword(s):  
Antibacterial Activity ◽  
Aeromonas Hydrophila ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Bacteria Growth ◽  
Activity Test ◽  
Inhibitory Power

This research aimed to assess the potential use of extracts of leaves, flowers, and stems of the balsamica plant increase inhibitory effect on the growth of Aeromonas hydrophila. The content of the plant contains a compound balsamica naphthoquinone, coumarin derivatives, tannins, flavonoids, and steroids. The active compounds have the ability as an antimicrobial. A hidrophyla bacteria, including opportunistic pathogens are almost always found in water and often cause disease when the fish in adverse conditions. Antibacterial activity test in this research using the spread plate method. Data obtained in the form of the extract, the results of bacterial inoculation, and the inhibition test results, will be analyzed, displayed with pictures and described descriptively. The result of the process of maceration extraction using ethanol 70% was obtained three extracts are concentrated leaf extract: 28.75 g, flower: 12.82 g, and rods: 29.48 g. The result of antibacterial extracts of leaves, stems, and flowers balsamica plant showed inhibitory activity on the A hydrophila bacteria that seems to be indicated by a clear zone around the paper disc. Based on the classification of leaf extract and extract of the stem can be classified in the class of strong, because the inhibition zone formed by the leaf extract is equal to 11.2 mm, and extract stem with 13.7 mm inhibition zone. Ability flower extract in inhibiting the growth of A hidrophila bacteria with 21.4 mm inhibition zone showed that the extract was included in a very strong class. Keywords: The ethanol extract balsamica, Impatiens balsamica L, Aeromonas hydrophila, antibacterial activity test.

scholarly journals Uji Antibakteri Sediaan Masker Peel Off Ekstrak Etanol Daun Alpukat (Persea ameicana Mill.) terhadap Bakteri Staphylococcus epidermidis sebagai Antijerawat

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 101
Author(s):  
Esterlina Aldora Puluh ◽  
Hosea Jaya Edy ◽  
Jainer Pasca Siampa
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Persea Americana ◽  
Inhibition Zone Diameter ◽  
Inhibitory Ability ◽  
Activity Test

Daun Alpukat memiliki kandungan antara lain saponin, alkaloid, flavonoid, polifenol, quersetin yang bersifat sebagai antibakteri.  Penelitian ini bertujuan untuk mendapatkan konsentrasi terbaik dari ekstrak etanol daun alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off berdasarkan uji aktivitas antibakteri terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode ekperimental laboratorium. Formula sediaan masker gel peel off ekstrak daun Alpukat dibuat dengan variasi konsentrasi 0.1%; 0.15%; 0.2%; 0.25% dan 0.3%. Ekstrak tanaman daun alpukat diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Pengujian antibakteri dengan metode sumuran diameter zona hambat yang paling besar yaitu pada konsentrasi 0,3% dengan diameter zona hambat 3 mm ± 0,32 dan kemampuan daya hambat dikategorikan lemah. Dapat disimpulkan bahwa konsentrasi terbaik dari ekstrak etanol daun Alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off yaitu pada konsentrasi 0.3% dan dikategorikan kemampuan daya hambat lemahAvocado leaves contains saponins, alkaloids, flavonoids, polyphenols, quercetin which are as antibacterial properties. This study aims to obtain the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) which is formulated as a gel peel off mask preparation based on antibacterial activity test against Staphylococcus epidermidis bacteria. This study uses a laboratory experimental method. Avocado leaf extract peel off mask gel formula is made with a concentration variation of 0.1%; 0.15%; 0.2%; 0.25% and 0.3%. Avocado leaf extract is obtained by maceration using 96% ethanol solvent. Antibacterial testing with the largest inhibition zone diameter wells method is at a concentration of 0.3% with a diameter of inhibition zone 3 mm ± 0.32 and the ability of inhibition is categorized as weak. It can be concluded that the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) Which is formulated as a gel peel off mask preparation is at a concentration of 0.3% and categorized as weak inhibitory ability

scholarly journals Antibacterial activity test of Simargaolgaol (Aglaonema modestum Schott ex Engl) leaves extract against Escherichia coli and Salmonella typhi bacteria

2021 ◽  
Vol 13 (2) ◽  
pp. 151-158
Author(s):  
Tiurma Solomasi Zega ◽  
◽  
Putri Mandaoni Pakpahan ◽  
Rahmayani Siregar ◽  
Givinda Sitompul ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Activity Test ◽  
Inhibitory Power ◽  
Ethanol Extracts

The Simargaolgaol (Aglaonema modestum Schott ex Engl) plant is a plant that grows wild in Barus District, Central Tapanuli, North Sumatra which is used by the community as a medicinal plant. This study aims to determine the antibacterial activity of n-hexane, ethyl acetate and ethanol extracts from Simargaolgaol leaves against Escherichia coli and Salmonella typhi bacteria. The antibacterial activity test of Simargaolgaol leaves was carried out using the disc diffusion method. The results of antibacterial activity showed that the highest inhibitory power of Simargaolgaol leaf extract was ethanol extract (polar), ethyl acetate extract (semi polar) and n-hexane extract (non polar). The inhibitory power of ethanol extract, ethyl acetate, and n-hexane against Escherichia coli bacteria was 13.1 mm (strong); 9.7 mm (medium); 8.0 mm (medium) and for Salmonella typhi bacteria respectively 11.2 mm (strong); 10.7 mm (strong) and 9.3 mm (medium). With the concentration of the extract in the diameter is 10%. Based on this, it can be concluded that Simargaolgaol leaf extract has potential as an antibacterial. Keywords: Aglaonema modestum Schott ex Engl, Antibacterial activity, Escherichia coli, Salmonella typhi

scholarly journals Formulation and evaluation of the nanosilver gel using bioreductor ethanol extract of katuk leaf (sauropus androgynus (l) merr) as antibacterial

2021 ◽  
Vol 10 (1) ◽  
pp. e2910111315
Author(s):  
. Nurfadilah ◽  
Yunahara Farida ◽  
. Faizatun
Keyword(s):  
Particle Size ◽  
Silver Nitrate ◽  
Antibacterial Agent ◽  
Leaf Extract ◽  
Synthesis Method ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Extract Concentration ◽  
Gel Preparation

Background: The current type of nanoparticle that attracts a lot of attention in the pharmaceutical field is Nanosilver because it can act as an antimicrobial. Objective: The aim of this study was to obtain the Nanosilver gel preparation from katuk leaf extract, which is effective as an antibacterial agent against acne. Methods: Katuk leaf extract remains as a reducing agent in the synthesis of Nanosilver using a green synthesis method. Nanosilver characterization was performed using PSA, FTIR and TEM. Results: Particle size of Nanosilver (Katuk leaf extract concentration 0.16% with a silver nitrate volume of 10 mM is 1: 9) of 164.40 nm, Nanosilver (Katuk leaf extract Concentration 0.32% with silver nitrate volume 10 mM is 1: 9) of 176.47 nm and Nanosilver (0.48% katuk leaf extract concentration with a volume of 10 mM silver nitrate of 1: 9) of 194.95 nm. Antibacterial activity using the Well method was shown that the nanosilver gel from Katuk leaf extract had an inhibitory effect on P. acnes bacteria with an inhibition zone of 26 mm  classified as very strong, and 12 mm was strongly categorized against S. aureus bacteria, namely in Formula 1. Conclusion: A variation in the concentration of katuk leaf extract influences the size of the particles formed. the Nanosilver katuk leaf extract has an antibacterial cause for acne with a very strong category.

scholarly journals Test Activities Antibakteriekstrak Ethanol Young And Old Leaves Of The Soursop(Annonamuricata L.) Against Staphylococcus aureus

2018 ◽  
Vol 1 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Dwi Latifah Sari ◽  
Sudarmi Sudarmi ◽  
Popi Patilaya
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Young Leaves

Soursop (Annona muricata L.) has been used for generations by some communities in Indonesia to treat diseases. Soursop leaves are used as an alternative treatment for cancer, by boiling them in the water and consuming it. In addition to the treatment of cancer, soursop plant is also used for the treatment of skin diseases such as boils. This study aimed to determine the antibacterial activity of ethanol extract of soursop leaves of young and old against Staphylococcus aureus, determine the content of secondary metabolites, and find out the diameter of inhibitory young leaves and old leaves of soursop in inhibiting Staphylococcus aureus. Soursop leaves were extracted by maceration using ethanol 96%. The extract obtained was then carried out to phytochemical screening. Antibacterial activity test was conducted using disc the Kirby-Bauerdiffusion. This study used 6 concentrations of 500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 80 mg/mL, 60 mg/mL, 40 mg/mL, 20 mg/mL, 10 mg/mL and 1 mg/mL. A positive control used soursop leaf extract of young and old while a negative one used DMSO 10%. Based on phytochemical screening, ethanol extract of soursop leaves contained alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids and glycosides. The antibacterial activity of ethanol extract of leaves of young soursop was higher in inhibitory diameter than the ethanol extract of old soursop leaves. It could be seen that a concentration of 500 mg/mL was equal to 10.87 mm and 8.68 mm; a concentration of 400 mg/mL was equal to 9.15 mm and 7.3 mm; a concentration of 300 mg/mL was equal to 8.34 mm and 6.30 mm; a concentration of 200 mg/mL was equal to 8.23 mm and 7.08 mm; a concentration of 100 mg/mL was equal to 6.32 mm and 6.18 mm, and a concentration of 80 mg/mL did not leave a diameter inhibitory to bacteria Staphylococcus aureus. Young soursop leaf ethanol extract was more effective than the old soursop one against Staphylococcus aureus.

scholarly journals Efek Antibakteri Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) dan Sediaan Masker Gel ¬Peel-Off Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 112
Author(s):  
Theresia H. Tunas ◽  
Hosea Jaya Edy ◽  
Jainer Pasca Siampa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Moringa Oleifera Leaves ◽  
Inhibitory Power ◽  
Moringa Oleifera Lam

Daun Kelor (Moringa oleifera Lam.) bermanfaat untuk menghambat aktivitas pertumbuhan bakteri karena adanya kandungan senyawa flavonoid , alkaloid, dan fenol. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari ekstrak etanol daun Kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental laboratorium, ekstrak etanol daun kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor dibuat dengan konsentrasi 1, 3, 5, 7, dan 9%. Ekstrak daun Kelor diperoleh dengan cara maserasi dengan menggunakan pelarut etanol 96%. Pada pengujian antibakteri menggunakan metode sumuran. Diameter zona hambat paling besar yang ditimbulkan oleh ekstrak etanol daun Kelor yaitu pada konsentrasi 7% dengan diameter zona hambat sebesar 5,75 mm, sedangkan pada sediaan masker gel peel-off tidak menunjukkan adanya daya hambat. Dapat disimpulkan bahwa ekstrak etanol daun Kelor memiliki aktivitas antibakteri yang termasuk dalam kategori sedang.Moringa oleifera Lam. (Moringa oleifera) leaves are useful for inhibiting bacterial growth activity due to the presence of flavonoid, alkaloid, and phenol compounds. This study aims to test the antibacterial activity of the Moringa leaf ethanol extract and the gel peel-off mask preparation of Moringa leaf ethanol extract against Staphylococcus aureus bacteria. This study uses laboratory experimental methods, Moringa leaf ethanol extract and gel mask peel-off preparation for Moringa leaf ethanol extract made with concentrations of 1, 3, 5, 7, and 9%. Moringa leaf extract was obtained by maceration using 96% ethanol solvent. In antibacterial testing using the well method. The biggest inhibition zone diameter caused by ethanol extract of Moringa leaves is at a concentration of 7% with a inhibition zone diameter of 5.75 mm, whereas the peel-off gel mask preparation did not show any inhibitory power. It can be concluded that the ethanol extract of Moringa leaves has antibacterial activity which is included in the medium category.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine americana Merr) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella typhi

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 525
Author(s):  
Rafi’a Adinda Putri ◽  
Herny E.I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Paper Disk ◽  
Eleutherine Americana

ABSTRACTDayak onions (Eleutherine americana Merr) has been used as a traditional plant which has  benefits  for  many  diseases,  one  of  them  is  antibacterial. This study aims to determine the growth inhibition of ethanol extract of Dayak onions leaves on the bacterium S. aureus, E. coli and  S. typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby-Bauer diffusion). The results showed that ethanol extract of bawang dayak has the potential as an antibacterial and has antibacterial strength against S. aureus, E. coli and S. typhi. At concentrations of 20%, 40% and 60% forming inhibition zone diameters (16.23 mm, 19.18 mm, 21.25 mm) S. aureus, (10.7 mm, 13.98 mm, 15.87 mm) E. coli, and  (14.43 mm, 14.6 mm, 17.2 mm) S. typhi. At concentration of 20%, 40% and 60% included strong groups to inhibit E. coli and  S. typhi, while at concentration of 60%, it is included in a very strong group to inhibit the S. aureus bacteria. Keywords: Antibacterial activity, Bawang Dayak , Kirby-Bauer diffusion method   ABSTRAK Bawang Dayak (Eleutherine americana Merr) telah digunakan sebagai tanaman tradisional yang bermanfaat bagi banyak penyakit salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ekstrak etanol bawang dayak terhadap bakteri S. aureus, E.coli dan S. typhi menggunakan tiga kosentrasi yaitu 20%, 40% dan 60%. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan  menggunkan metode difusi dengan cakram kertas (difusi Kirby-Bauer). Hasil penelitian menunjukkan bahwa ekstrak etanol bawang dayak berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap S. aureus, E. coli dan S. typhi. Pada konsentrasi 20%, 40% dan 60% membentuk diameter zona hambat (16.23 mm, 19.18 mm, 21.25 mm) pada S. aureus, (10.7 mm, 13.98 mm, 15,87 mm) pada E. coli, (14.43 mm, 14.6 mm, 17.2 mm) pada S. typhi. Pada konsentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri E. coli dan S. typhi, sedangkan pada konsentrasi 60% termasuk dalam golongan sangat kuat untuk menghambat bakteri S. aureus. Kata kunci: Aktivitas antibakteri, Bawang Dayak, metode difusi Kirby-Bauer

scholarly journals Evaluasi Aktivitas Antibakteri Sediaan Plester Ekstrak Daun Petai Cina (Leucaena Leucocephala) Terhadap Bakteri Staphylococcus AureusAttc 25923 Pk/5

2021 ◽  
Vol 1 ◽  
pp. 665-670
Author(s):  
Eka Sri Rahayu ◽  
W Wirasti ◽  
S Slamet ◽  
Dwi Bagus Pambudi
Keyword(s):  
Antibacterial Activity ◽  
Leucaena Leucocephala ◽  
Leaf Extract ◽  
Dosage Form ◽  
Inhibition Zone ◽  
Test Method ◽  
Inhibitory Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Inhibitory Power

AbstractChinese petai plants contain alkaloids that have antibacterial abilities including Staphylococcus aureusATTC 25923 PK/5 bacteria. Plaster is a dosage form whose active ingredients need to be developed from natural ingredients, especially Chinese petai leaves. The purpose of this study was to optimize the plaster of Chinese petai leaf extract and antibacterial Staphylococcus aureusATTC 25923 PK/5and to determine the antibacterial activity of the extract plaster with various doses. The test method for antibacterial is the well method. The data obtained is the diameter of the inhibitory power of the extract in the plaster. Data analysis to determine antibacterial activity using spss. The results of the phytochemical test showed that the Chinese petai leaf extract contained active compounds, namely tannins, saponins, alkaloids, flavonoids and steroids. Chinese petai leaf extract was also able to inhibit Staphylococcus aureusATTC 25923 PK/5bacteria in the extract obtained an inhibition zone of 5.1 mm at a concentration of 25% and plaster obtained an inhibition zone of 3.46 mm, a concentration of 50% obtained an inhibitory zone diameter of 5.63 mm and at plaster obtained an inhibition zone diameter of 4.5 mm, a concentration of 75% obtained an extract inhibition zone diameter of 6.56 mm on plaster obtained an inhibition zone diameter of 5.53 mm, a concentration of 100% obtained an extract inhibition zone diameter of 7 mm and on plaster The diameter of the inhibition zone was 6.46 mm. This shows that the Chinese petai leaf extract has potential as an antibacterial against the growth of Staphylococcus aureusATTC 25923 PK/5bacteria.Keywords: Chinese Petai, Extract, Plaster, Antibacterial AbstrakTanaman petai cina mengandung alkaloida yang memiliki kemampuan sebagai antibakteri diantaranya bakteri Staphylococcus aureusATTC 25923 PK/5. Plester merupakan bentuk sediaan yang bahan aktifnya perlu dikembangkan dari bahan alam terutama daun petai cina. Tujuan penelitian ini melakukan optimasi plester ekstrak daun petai cina dan antibakteri Staphylococcus aureusATTC 25923 PK/5dan mengetahui aktivitas antibakteri plester ekstrak dengan berbagai dosis. Metode uji terhadap antibakteri adalah metode sumuran. Data yang didapatkan adalah diameter daya hambat ekstrak dalam plester. Analisis data untuk mengetahui aktivitas antibakteri menggunakan ANOVA. Hasil uji fitokimia menunjukkan bahwa ekstrak daun petai cina memiliki kandungan senyawa aktif, yakni tanin, saponin, alkaloid, flavonoid dan steroid. Ekstrak daun petai cina juga mampu menghambat bakteri Staphylococcus aureusATTC 25923 PK/5pada ekstrak didapatkan zona hambat sebesar 5,1 mm pada konsentrasi 25% dan plester didapatkan zona hambat sebesar 3,46 mm, konsentrasi 50%didapatkan diameter zona hambat ekstrak sebesar 5,63 mm dan pada plester didapatkan diameter zona hambat sebesar 4,5 mm, konsentrasi 75% didapatkan diameter zona hambat ekstrak sebesar 6,56 mm pada plester didapatkan diameter zona hambat sebesar 5,53 mm, konsentrasi 100% didapatkan diameter zona hambat ekstrak sebesar 7 mm dan pada plester didapatkan diameter zona hambat sebesar 6,46 mm. Hal ini menunjukkan bahwa ekstrak daun petai cina memiliki potensi sebagai antibakteri terhadap pertumbuhan bakteri Staphylococcus aureusATTC 25923 PK/5.Kata kunci:Petai Cina, Ekstrak, Plester, Antibakteri

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Etanol Daun Ketapang (Terminalia catappa L.) Warna Hijau dan Warna Merah serta Kombinasinya

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Munira Munira Munira ◽  
Rasidah Rasidah Rasidah ◽  
Eva Melani Melani ◽  
Noni Zakiah Zakiah ◽  
Muhammad Nasir Nasir
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
Green Leaf ◽  
Diffusion Method ◽  
Bacteria Growth ◽  
Terminalia Catappa

ABSTRAKDaun ketapang (Terminalia catappa L.) adalah tanaman yang sering dimanfaatkan oleh masyarakat sebagai tanaman obat tradisional. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak etanol daun ketapang warna hijau, warna merah, kombinasi daun warna hijau dan daun warna merah terhadap pertumbuhan bakteri Staphylococcus aureus. Penelitian ini bersifat eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan yaitu P0 (aquadest sebagai kontrol negatif), P1 (ekstrak etanol daun hijau), P2 (ekstrak etanol daun merah), dan P3 (kombinasi ekstrak etanol daun ketapang berwarna hijau dan merah) dan masing-masing 3 kali ulangan. Uji mikrobiologi menggunakan metode difusi. Berdasarkan hasil uji Anova menunjukkan bahwa ekstrak daun ketapang sangat berpengaruh terhadap pertumbuhan Staphylococcus aureus (P=0,000).Berdasarkan hasil uji lanjut Duncan rata-rata diameter zona hambat yang terbentuk menunjukkan bahwa kombinasi ekstrak daun ketapang warna hijau dan warna merah memiliki zona hambat yang lebih besar (30,92 mm) dan berbeda nyata dengan ekstrak daun ketapang berwarna hijau (21,92 mm), akan tetapi tidak berbeda nyata dengan ekstrak daun ketapang warna merah (26,42 mm).Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak daun ketapang dapat menghambat pertumbuhan bakteri Staphylococcus aureus.Kata kunci: Terminalia catappa L., diameter zona hambat, Staphylococcus aureus Ketapang leaf (Terminalia catappa L.) is a plant that is often used by the community as a traditional medicinal plant. This research was conducted to determine the effect of ethanol extract of green ketapang leaves, red color, combination of green leaf and red leaf color to Staphylococcus aureus bacteria growth. This research was experimental by using Completely Randomized Design (RAL) with 4 treatments which is  P0 (aquadest as negative control), P1 (green leaf ethanol extract), P2 (red leaf ethanol extract), and P3 (combination of green leaf ketapang ethanol extract and red) and each of them repeated 3 times. Microbiology test using diffusion method. Based on the results of Anova test showed that ketapang leaf extract greatly affect the growth of Staphylococcus aureus (P=0.000). Based on the results of further test Duncan average diameter of the inhibition zone that was formed showed that the combination of green leaf and red colored leaf extract has a larger inhibition zone (30.92 mm) and significantly different with green leaf ketapang leaf extract (21.92 mm), but not significantly different with the extract of red leaf ketapang (26.42 mm). From the results of this study can be concluded that ketapang leaf extract can inhibit the growth of Staphylococcus aureus.Keywords: Terminalia catappa L., inhibition zone diameter, Staphylococcus aureus 

scholarly journals Aktivitas Antibakteri Ekstrak Daun Widuri Terhadap Escherichia Coli Dan Staphylococcus Aureus

2020 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Siti Zamilatul Azkiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
The Body ◽  
And Staphylococcus Aureus

Antibiotics have a very important role in fighting bacterial infections in the body. However, the results given so far have not been optimal in overcoming the problem of bacterial infection. An alternative solution is to develop and study plants that have medicinal potential, for example thistle leaves (Calotrophis gigantean). The purpose of this study was to determine the antibacterial activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus. This research is a laboratory-scale experimental research that is to test the activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus including sample identification, extraction, activity testing by observing the inhibition zone of the two tested bacteria. Based on the results of the study stated that the ethanol extract of 70% Widuri leaves (Calotrophis gigantea) had antibacterial activity and could inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria. The active compounds that are thought to have antibacterial properties in the ethanol extract of Widuri leaves (Calotrophis gigantea) are flavonoids, alkaloids and terpenoids.   Keywords: Widuri leaves, Escherichia coli, Staphylococcus aureus, Antibacterial ABSTRAK   Pentingnya penanggulangan infeksi bakteri menggunakan antibiotik adalah hal yang lazim dalam terapi farmakologi. Dampak dari pemberian antibiotik masih belum maksimal dalam penanggulangannya terhadap infeksi bakteri. Pemilihan alternatif bahan alam dengan kandungan kimia yang memiliki potensi sebagai antibiotik dapat menjadi pilihan lain dalam mengurangi penggunaan antibiotik sintetik, misalnya tanaman widuri (Calotrophis gigantean). Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri ekstrak daun widuri terhadap Escherichia coli dan Staphylococcus aureus. Desain penelitian meliputi identifikasi sampel, ekstraksi, hingga uji aktivitas dengan mengamati zona hambat dari kedua bakteri uji. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% daun Widuri memiliki aktivitas antibakteri dan dapat menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Senyawa aktif yang diduga bersifat antibakteri pada ekstrak etanol daun Widuri adalah senyawa golongan flavonoid, alkaloid dan terpenoid. Kata Kunci: Daun Widuri, Escherichia coli, Staphylococcus aureus, Antibakteri

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