scholarly journals The Effect of Betel Quid Extract on Wound Healing Process in Male Wistar Rats (Rattus norvegicus L.)

2019 ◽  
Vol 7 (6) ◽  
Author(s):  
Siti Rusdiana Puspa Dewi ◽  
Adelina Fatonah
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Positive Control ◽  
Betel Quid ◽  
Wound Healing Process ◽  
Lower Lip ◽  
Proliferative Phase ◽  
Inflammatory Phase ◽  
Male Wistar Rats

Wound can be occured during dental treatment. In outline, there are several phases of wound healing; inflammatory phase, proliferative phase, and maturation phase. Various drugs in various forms are used to accelerate the healing process, but mostly they have side effects. Therefore, traditional medicine, such as betel quid consisted of betel leaf, areca nut, gambier, and calcium hydroxide, is developed. The aim of this study was to investigate the effect of wound healing process in male Wistar rats. A total of 30 male Wistar rats were taken and divided into 5 groups:  Group 1, 2, and 3 (K1,K2, K3)  were given 5%, 10% and 15% concentrations of betel quid extract oinments; Group 4 (K4) was positive control  (hyaluronic acid 0.2% oinment); Group 5 (K5) was negative control (placebo oinment). One mm- diameter of lower lip mucosal wounds on rats were created by using  cylinder diamond bur. The oinments were applied twice daily for 10 days. The number of netrophils on first and third day were measured and the thickness of ephitelium on 10 days were determined. All groups of  betel quid extracts exhibited the reduction of the number of neutrophils on inflammatory phase. Group 3 shown as the highest effect and had no significant different with positive control. Betel quid extracts in all groups also improved epithelial thickness on proliferative phase, in which group II and group III had no significant different with positive control. Betel quid extract had effect on wound healing process in male Wistar rats due to its ability in supressing inflamation and in increasing reepithelization.

scholarly journals Esktrak ikan haruan (Channa striata) menurunkan jumlah makrofag pada fase inflamasi proses penyembuhan luka (Extract of haruan (Channa striata) decreases macrophages count in inflammation phase of wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 6
Author(s):  
Putri Sri Hartini ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Wistar Rats ◽  
Healing Process ◽  
Positive Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Post Test ◽  
Channa Striata

Haruan is a kind of freshwater fishes commonly found in South Kalimantan. Haruan extract contains nutritional substancessuch as albumin, Zn, Fe, Cu, and unsaturated fatty acid which can help accelerate wound healing. The aim of this studywas to histopathologically assess the effect of 100%, 50%, and 25%haruan extract compared to aquadest and ibuprofenon macrophages count in inflammation phase of wistar rats’ buccal mucosa wound healing. This research was a trueexperimental with post test-only with control design. Samples used were 30 wistar rats divided into 5 groups, 100%, 50%,25% haruan extract treatment groups, ibuprofen treatment group as positive control, and aquadest treatment group asnegative control. Mean macrophages counted on day 3 of 100%, 50%, 25% haruan extract treatment groups aquadest,and ibuprofenl were 2.05, 4.4, 3.9, 3.3, 2.4 respectively. In conclusion, haruan extract had a significant effect in decreasingmacrophages count in inflammation phase of wound healing.

scholarly journals Ekstrakharuan(Channa striata)secara efektif menurunkan jumlah limfosit fase inflamasi dalam penyembuhan luka (Extract of haruan (Channa striata) decreases lymphocyte count in inflammatory phase of wound healing process effectively)

2014 ◽  
Vol 13 (3) ◽  
pp. 176
Author(s):  
Arisa Izzaty ◽  
Nurdiana Dewi ◽  
Dewi Indah Noviana Pratiwi
Keyword(s):  
Wound Healing ◽  
Lymphocyte Count ◽  
Healing Process ◽  
Simple Random Sampling ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Inflammatory Phase ◽  
Channa Striata

Haruan is river-water fish usually consumed by South Kalimantan people. Haruan extract contains albumin, Zn, Fe,Cu and unsaturated fatty acid which can act as antiinflammatory substances and accelarate wound healing. The aim ofthis study is to examine effectiveness of haruan extract on lymphocyte count in infammatory phase of wound healingprocess and compare it to ibuprofen. This study used true experimental method with post-test only control group designand simple random sampling. Samples were divided into 5 treatment groups:which were given the extract for 7 sevendays: 25% haruan extract group, 50% haruan extract group, 100% haruan extract group, negative control K(-) usingaquadest, positive control K(+) given ibuprofen suspension with dosage of 20mg/kg weight/day. Lymphocyte cells wereobserved using microscope and counted per field of view. Lymphocyte count reached its peak on day 5. Lymphocytecount means in each group were 4,2 ± 0,566; 1± 0,000; 3,8 ± 2,546; 7,1 ± 0,141; dan 7,5 ± 3,818 respectively. Two way ANOVA and post hoc LSD tests showed that lymphocyte count significantly decreased in 50% and 100% treatmentgroups (p<0,05) and has equal effect with ibuprofen in decreasing lymphocyte count. This study concluded that haruanextract significantly decreased lymphocyte count in wound healing process.

scholarly journals Effectivity of Camellia sinensis Extract with Nanochitosan to Fibroblast Amounts of Wistar Rats Gingival Wound Healing Process

2021 ◽  
Vol 16 (Supp. 1) ◽  
pp. 25-32
Author(s):  
Indra Bramanti ◽  
Annisa Hidaratri Uningojati ◽  
Dilla Asriyani ◽  
Urfa Tabtila ◽  
Fathul Muin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Wistar Rats ◽  
Drug Carrier ◽  
Healing Process ◽  
Green Tea Extract ◽  
Wound Healing Process ◽  
Proliferative Phase ◽  
Tea Extract

Green tea (Camellia sinensis) has high level of flavonoids which are proven to have anti-inflammatory activity. Effect of flavonoids can be enhanced by nano-chitosan capsulation as drug carrier. Chitosan is polysaccharide derived from crustacean shells that mostly used as matrix of various drugs and plant extracts. The aim of this study was to determine the effectivity of flavonoids in green tea extract in nanochitosan capsulation towards the number of fibroblasts on proliferative phase of gingival wound healing process. Green tea was extracted, encapsulated with nano-chitosan and then made into gel. Gingiva labial of 24 male white 3-month-old Wistar rats were wounded by punch biopsy (2 mm diameter), then were treated two times a day, and were divided randomly into four groups of topical gel applications: green tea extract gel encapsulated nano-chitosan, green tea extract gel, base gel as negative control, and NSAIDs gel as positive control, starting at 0 day until 7th day. At 5th and 7th day, three rats from each group were decapitated and the mandibular gingiva was taken in order to make histology slides with hematoxylin eosin staining. Under microscope, the number of fibroblasts were examined. The data were analysed using ANOVA test with 95% confidence level. The results showed that the number of fibroblasts on proliferative phase was significantly higher than control negative (p < 0.05) and has no significant differences (p > 0.05) with control positive. In conclusion, topical application of green tea extract gel encapsulated nano-chitosan was effective to accelerate rats gingival wound healing process by increasing the fibroblasts.

scholarly journals Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in wistar rats

2016 ◽  
Vol 49 (3) ◽  
pp. 125
Author(s):  
Gunawan Oentaryo ◽  
Istiati Istiati ◽  
Pratiwi Soesilawati
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Tissue Growth ◽  
Control Group ◽  
Wound Healing Process ◽  
Lower Lip ◽  
Treatment Groups ◽  
Collagen Secretion ◽  
Channa Striata

Background: Wound healing is a biological process associated with tissue growth and regeneration. Wound healing process, is important to repair damaged tissue. Wound healing process consists of coagulation and hemostasis, inflammation, proliferation, as well as remodeling phases. The process can be accelerated by taking synthetic or non synthetic drugs. One of them is Channa striata extract. The extract contains albumin, copper, and zinc, which can be assumed to increase inflammatory cell infiltration, fibroblast proliferation, and collagen secretion. Purpose: This study aimed to reveal the effects of Channa striata extracts on fibroblast number and FGF-2 expression in mucosal wound healing process of the Wistar rats’ lower lip. Method: This research was a true laboratory experimental research with randomized post test only control group design. Samples of experiment were devided to experiment and control group that consist five samples each. Experimental group was treted with Channa striata extract and ethanol at concentration of 25%, 50%, and 100%. The fibroblast number and FGF-2 expresion were examined. Result: The number of fibroblasts in the treatment groups receiving Channa striata extract at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest number of fibroblasts was found on day 3 at the concentration of 100% (p<0.05). Similarly, FGF-2 expression in the treatment groups receiving Channa striata at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest expression of FGF-2 was found on day 3 at the concentration of 50% (p<0.05). Conclusion: Channa striata extract increased fibroblast number and FGF-2 expression in mucosa wound healing process.

scholarly journals Neutrophil count in the gingival wound healing process after apitoxin gel application (gingival wound healing model on Wistar rats)

2021 ◽  
Vol 6 (2) ◽  
pp. 65
Author(s):  
Urfa Tabtila ◽  
Swastiana Eka Yunita ◽  
Muhammad Nabil Pratama ◽  
Juni Handajani
Keyword(s):  
Wound Healing ◽  
Periodontal Disease ◽  
Neutrophil Count ◽  
Wistar Rats ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Punch Biopsy ◽  
Bee Venom ◽  
Wound Healing Process ◽  
Male Wistar Rats

Periodontal disease has a high prevalence in Indonesia. Gingival disease is a periodontal disease if it is severe, requires surgical therapy called a gingivectomy. Surgical periodontal procedures lead to a gingival wound that is usually treatedusing a periodontal pack. Periodontal pack has no curative property in general but assists in a wound healing process by protecting tissues. Neutrophils are inflammatory cells that act as a host’s first defense against infectious pathogens and they are important in wound healing. Wound healing can be accelerated by apitoxin gel from honeybee Apis mellifera species. The aim of this study was to determine the effectiveness of apitoxin gel on neutrophil count on gingival woundhealing process. Medical bee venom powder (Umea, Sweden) was mixed with double-distilled water to prepare a concentration of 0.01% bee venom. The solution was mixed with 10% propylene glycol, 0.01% methylparaben, and3% CMC-sodium. Thirty six gingiva of 3 month-old male Wistar rats (weighing 200-250 gram) were injured using punch biopsy (3 mm in diameter). They were treated in 4 groups: apitoxin gel, apitoxin gel with a periodontal pack, NSAIDsFlamar® gel with periodontal pack, and a periodontal pack. Three Wistar rats from each group were euthanized and their jaws were taken after 48 hours treatment to make histology slides with Hematoxylin Eosin (HE) staining. Neutrophil counts were observed. The data were analyzed using Anova test with 95% confidence level. The results showed there were significan defferebces among 4 groups (p<0.05). The highest neutrophil count on the 2nd day was found in the apitoxin gel induced the 2 day of gingival wound healing process.

scholarly journals Anti Inflammatory Effect Of Toman Fish (Channa micropeltes) Extract In Wound Healing Process

2018 ◽  
Vol 14 (2) ◽  
pp. 83
Author(s):  
Maharani Laillyza Apriasari ◽  
Dewi Puspitasari ◽  
Amy Nindia Carabelly
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Healing Process ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Wound Healing Process ◽  
Inflammatory Phase ◽  
Anti Inflammatory ◽  
Inflammatory Effect

 Abstract: One phase of wound healing is the inflammatory phase. Haruan fish extract has shown to accelerate the healing process up because it has anti-inflammatory effects. Toman fish originates from the same genus as the Haruan fish, but its anti-inflammatory effect remains unknown. It was done to analyzed anti-inflammatory effects of Toman fish extract. This study was true experimental design with posttest-only control design. Twenty-seven male mice of Balb-C strain were divided into 3 groups. Incision wound of 1 cm was made along the back of the mice. Nine mice in each group were sacrificed on 3rd days, then histopathology examination was conducted with Haematoxylin eosin. There were significant differences between treatment group given Toman fish extract and positive control group given Haruan fish extract, and also compared to the negative control group in PMN cell examination. There were significant differences between treatment group given Toman fish extract and positive control group given Haruan fish extract compared to negative controls on the number of macrophage cells. Toman fish extract could lower the number of PMN cells and was able to increase the number of macrophages on the 3rd days. Toman fish has anti-inflammatory effects on the wound healing process. Keywords: Anti-inflammatory effects, haruan fish extract, macrophages, PMN cells, toman fish extract

scholarly journals FORMULATION OF GEL FROM GYNURA SEGETUM EXTRACT AND ITS ACTIVITY ON BURN WOUND HEALING

2021 ◽  
pp. 269-271
Author(s):  
ARIF BUDIMAN ◽  
MUHARAM ◽  
ANIDA CRISTI MAULIDA ◽  
DIAH LIA AULIFA
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Physical Stability ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Hot Plate ◽  
Topical Preparation ◽  
Burn Wound Healing ◽  
Male Wistar Rats

Objective: The aims of this study were to formulate gel from Gynura segetum (GS) extract and evaluate its burn-healing activity. Methods: GS extract was formulated using carbomer and carboxymethylcellulose (CMC) as a stabilizer with various concentrations. Furthermore, the gel of GS extract was evaluated, including organoleptic, pH and viscosity. A burn-healing evaluation was conducted by making a wound with a hot plate on male Wistar rats, and 600 mg of the gel was applied. Subsequently, the presence of erythema and width contraction of the burns were observed for 15 d. Results: The result showed that the formulation containing 1 % of carbomer and 1.2 % of GS extract has the best physical stability. The gel also increased the rate of the healing process with decreased burn wound contraction (5.67 mm after 15 d) and the erythema than the control (8.50 mm after 15 d). The significance value was less than 0.05, indicating that the rate of the healing process was significantly different between the GS extract gel and the control. Conclusion: This finding demonstrated that the gel of GS extract can significantly improve the burns wound healing process and may also be safely used for topical preparation.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Production of vegetable oil blends and structured lipids and their effect on wound healing

2015 ◽  
Vol 51 (2) ◽  
pp. 415-427 ◽  
Author(s):  
Juliana Neves Rodrigues Ract ◽  
Fabiana Andreia Schäfer De Martini Soares ◽  
Hosana Gomes Rodrigues ◽  
José Ricardo Bortolon ◽  
Gilson Masahiro Murata ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Structured Lipids ◽  
Physiological Saline ◽  
Structured Lipid ◽  
Control Group ◽  
Wound Healing Process ◽  
Oil Blends ◽  
Inflammatory Phase ◽  
Canola Oils

<p>Two oil blends (sunflower/canola oils 85/15 (BL1) and canola/linseed oils 70/30 (BL2)), were prepared and enzymatically interesterified to be applied to surgically-induced wounds in rats. Following surgery, the animals were submitted to the Treatment with Physiological Saline (TPS) (control group), Blends (TBL), and Structured Lipids (TSL). The control group (TPS) received physiological saline solution for 15 days. In TBL, BL1 was administered during the inflammation phase (days 0-3) and BL2 in the tissue formation and remodeling phase (days 4-15). In TSL, Structured Lipid 1 (SL1) and Structured Lipid 2 (SL2) were used instead of BL1 and BL2, respectively. The aim of this study was to compare wound closure evolution among rats treated with the blends or structured lipids versus control rats treated with physiological saline. The wound healing process was evaluated by measuring the wound areas along the treatments and the concentrations of cytokines. An increase in the areas of wounds treated with the blends and structured lipids in the inflammatory phase was observed, followed by a steeper closure curve compared to wounds treated with physiological saline. The changes observed during the inflammatory phase suggest a potential therapeutic application in cutaneous wound healing which should be further investigated.</p>

scholarly journals Efektivitas Gel Ekstrak Bawang Putih terhadap Proses Penyembuhan Luka Fase Inflamasi

2018 ◽  
Vol 4 (2) ◽  
pp. 109
Author(s):  
Mufimah Mufimah ◽  
Uti Rusdian Hidayat ◽  
Ichsan Budiharto
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
Garlic Extract ◽  
Group Method ◽  
Control Group ◽  
Wound Healing Process ◽  
Protection Mechanism ◽  
Inflammatory Phase ◽  
Post Test

Abstract: Efectiveness Gel Extract Of White On The Process Of Healing Inflamation Phase Heating. The inflammatory phase is a favorable body response as a protection mechanism. In the process of wound healing becomes a very important phase. Management of inflammation that is often used Non-Steroid Anti-Inflammatory class of salicylates on the skin that have side effects. The content of allicin in garlic can be used for problems that begin with the inflammatory phase. The use of gel from garlic extract is also easier to use and easier to clean. The study aim to determine the effectiveness of garlic extract gel to process wound inflammatory phase healing. This research is an experimental research with pre and post test with control group method with 24 samples. Conducted injury to the back area of rat length of wound 1 cm, depth to dermis. Conducted wound care, given gel extract of garlic concentration of 20%, 40%, 80% of the control using 0.9% NaCl compress. Using Kruskal Wallis test and Anova oneway showed concentration of 20%, 40%, and 80% of sig <0,05 ie 0.00. It was concluded that 20%, 40%, 80% garlic extract gel was effective against inflammatory wound healing process. The use of garlic extract gel is more effective in the wound inflammatory wound healing process.Abstrak: Efektivitas Gel Ekstrak Bawang Putih  terhadap Proses Penyembuhan Luka Fase Inflamasi.  Fase inflamasi merupakan respon tubuh yang menguntungkan sebagai mekanisme perlindungan. Pada proses penyembuhan luka menjadi fase yang sangat penting. Penatalaksanaan inflamasi yang sering digunakan Anti-Inflamasi Non Steroid golongan salisilat pada kulit yang memiliki efek samping. Kandungan zat allicin pada bawang putih dapat dimanfaatkan untuk masalah yang diawali dengan fase inflamasi. Pemanfaatan gel dari ekstrak bawang putih pun dalam penggunaannya lebih mudah diabsorsi dan mudah dibersihkan. Penelitian ini bertujuan untuk mengetahui efektifitas gel ekstrak bawang putih terhadap proses penyembuhan luka fase inflamasi. Penelitian ini merupakan penelitian eksperiment dengan metode pre and post test with control grup dengan jumlah sampel sebanyak 24 ekor tikus. Dilakukan perlukaan pada daerah punggung tikus panjang luka 1 cm, kedalaman sampai dermis. Dilakukan perawatan luka, diberi gel ekstrak bawang putih konsentrasi berbeda yaitu 20%, 40%, 80%  kontrol menggunakan kompres NaCl 0,9%. Hasil uji Kruskal Wallis dan Anova oneway menunjukkan konsentrasi 20%, 40%, dan 80%  nilai sig <0,05 yaitu 0,00. Disimpulkan bahwa 20%, 40%, 80% gel ekstrak bawang putih efektif terhadap proses penyembuhan luka inflamasi. Penggunaan gel ekstrak bawang putih lebih efektif dalam proses penyembuhan luka inflamasi luka.   Disimpulkan bahwa 20%, 40%, 80% gel ekstrak bawang putih efektif terhadap proses penyembuhan luka inflamasi. Penggunaan gel ekstrak bawang putih lebih efektif dalam proses penyembuhan luka inflamasi luka.

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