scholarly journals Effectivity of Camellia sinensis Extract with Nanochitosan to Fibroblast Amounts of Wistar Rats Gingival Wound Healing Process

2021 ◽  
Vol 16 (Supp. 1) ◽  
pp. 25-32
Author(s):  
Indra Bramanti ◽  
Annisa Hidaratri Uningojati ◽  
Dilla Asriyani ◽  
Urfa Tabtila ◽  
Fathul Muin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Wistar Rats ◽  
Drug Carrier ◽  
Healing Process ◽  
Green Tea Extract ◽  
Wound Healing Process ◽  
Proliferative Phase ◽  
Tea Extract

Green tea (Camellia sinensis) has high level of flavonoids which are proven to have anti-inflammatory activity. Effect of flavonoids can be enhanced by nano-chitosan capsulation as drug carrier. Chitosan is polysaccharide derived from crustacean shells that mostly used as matrix of various drugs and plant extracts. The aim of this study was to determine the effectivity of flavonoids in green tea extract in nanochitosan capsulation towards the number of fibroblasts on proliferative phase of gingival wound healing process. Green tea was extracted, encapsulated with nano-chitosan and then made into gel. Gingiva labial of 24 male white 3-month-old Wistar rats were wounded by punch biopsy (2 mm diameter), then were treated two times a day, and were divided randomly into four groups of topical gel applications: green tea extract gel encapsulated nano-chitosan, green tea extract gel, base gel as negative control, and NSAIDs gel as positive control, starting at 0 day until 7th day. At 5th and 7th day, three rats from each group were decapitated and the mandibular gingiva was taken in order to make histology slides with hematoxylin eosin staining. Under microscope, the number of fibroblasts were examined. The data were analysed using ANOVA test with 95% confidence level. The results showed that the number of fibroblasts on proliferative phase was significantly higher than control negative (p < 0.05) and has no significant differences (p > 0.05) with control positive. In conclusion, topical application of green tea extract gel encapsulated nano-chitosan was effective to accelerate rats gingival wound healing process by increasing the fibroblasts.

scholarly journals Effect of green tea extract (Camellia sinensis) on the spermatic parameters of Wistar rats submitted or not to testicular heat shock

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Joane Isis Travassos Vieira ◽  
Taylane Alves da Silva ◽  
Williane Maria Pereira Barbosa ◽  
Gabriela Lima de Azevêdo ◽  
Lúcia Cristina Pereira Arruda ◽  
...  
Keyword(s):  
Heat Shock ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Wistar Rats ◽  
Green Tea Extract ◽  
Tea Extract

scholarly journals Circulating Hypoxia Responsive microRNAs (HRMs) and Wound Healing Potentials of Green Tea in Diabetic and Nondiabetic Rat Models

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Hadeel A. Al-Rawaf ◽  
Sami A. Gabr ◽  
Ahmad H. Alghadir
Keyword(s):  
Wound Healing ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Green Tea Extract ◽  
Pcr Analysis ◽  
Diabetic Wound ◽  
Diabetic Wound Healing ◽  
Tea Extract

Green tea (Camellia sinensis) has many biological activities and may promote diabetic wound healing by regulation of circulating hypoxia responsive microRNAs (HRMs) which triggers the wound repairing process in diabetic and nondiabetic wounds. Thus, in this study, the potential effects of green tea extract (GTE) on the expression of miRNAs; miR-424, miR-199a, miR-210, miR-21, and fibrogenitic markers; hydroxyproline (HPX), fibronectin (FN), and nitric oxide (NO) were evaluated in wounds of diabetic and nondiabetic rats. The animals were topically treated with vaseline, 0.6% GTE, and 5%w/w povidone iodine (standard control). HPX, FN, and NO levels and microRNAs, miR-424, miR-210, miR-199a, and miR-21, were estimated in wound tissues using colorimetric, immunoassay, and molecular PCR analysis. In vitro analysis was performed to estimate active constituents and their antioxidant activities in methanolic green teat extract (GTE). Wounds treated with green tea, a dose of 0.6, healed significantly earlier than those treated with standard vehicle and vaseline treated diabetic wounds. Higher expressions of HRMs, miR-199a, and miR-21, and lower expression of HRMs, miR-424 and miR-210, were significantly reported in tissues following treatment with green tea extract compared to standard control vehicle. The tissues also contained more collagen expressed as measures of HPX, FN, and NO and more angiogenesis, compared to wounds treated with standard control vehicle. Diabetic and nondiabetic wounds treated with green tea (0.6%) for three weeks had lesser scar width and greater re-epithelialization in shorter periods when compared to standard control vehicle. Expression of HRMs, miR-199a, miR-21, and HRMs and miR-424 and miR-210 correlated positively with HPX, fibronectin, NO, better scar formation, and tensile strength and negatively with diabetes. In addition to antidiabetic and antioxidant activities of green tea components, GTE showed angiogenesis promoting activity in diabetic wound healing. In conclusion, Camellia sinensis extracts in a dose of 0.6% significantly promote more collagen and fibronectin deposition with higher expression of NO, promoting angiogenesis process via molecular controlling of circulating hypoxia responsive microRNAs: miR-424, miR-210, miR-199a, and miR-21 in diabetic and nondiabetic wounds. Our results support a functional role of circulating hypoxia responsive microRNAs: miR-424, miR-210, miR-199a, and miR-21 as potential therapeutic targets in angiogenesis and vascular remodeling in diabetic wound healing.

scholarly journals The Effect of Betel Quid Extract on Wound Healing Process in Male Wistar Rats (Rattus norvegicus L.)

2019 ◽  
Vol 7 (6) ◽  
Author(s):  
Siti Rusdiana Puspa Dewi ◽  
Adelina Fatonah
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Positive Control ◽  
Betel Quid ◽  
Wound Healing Process ◽  
Lower Lip ◽  
Proliferative Phase ◽  
Inflammatory Phase ◽  
Male Wistar Rats

Wound can be occured during dental treatment. In outline, there are several phases of wound healing; inflammatory phase, proliferative phase, and maturation phase. Various drugs in various forms are used to accelerate the healing process, but mostly they have side effects. Therefore, traditional medicine, such as betel quid consisted of betel leaf, areca nut, gambier, and calcium hydroxide, is developed. The aim of this study was to investigate the effect of wound healing process in male Wistar rats. A total of 30 male Wistar rats were taken and divided into 5 groups:  Group 1, 2, and 3 (K1,K2, K3)  were given 5%, 10% and 15% concentrations of betel quid extract oinments; Group 4 (K4) was positive control  (hyaluronic acid 0.2% oinment); Group 5 (K5) was negative control (placebo oinment). One mm- diameter of lower lip mucosal wounds on rats were created by using  cylinder diamond bur. The oinments were applied twice daily for 10 days. The number of netrophils on first and third day were measured and the thickness of ephitelium on 10 days were determined. All groups of  betel quid extracts exhibited the reduction of the number of neutrophils on inflammatory phase. Group 3 shown as the highest effect and had no significant different with positive control. Betel quid extracts in all groups also improved epithelial thickness on proliferative phase, in which group II and group III had no significant different with positive control. Betel quid extract had effect on wound healing process in male Wistar rats due to its ability in supressing inflamation and in increasing reepithelization.

scholarly journals Streptococcus Alpha growth in gingivitis patient’s dental plaque after rinsing with green tea extract (Camellia Sinensis)

2007 ◽  
Vol 19 (1) ◽  
Author(s):  
Isti Endah Kurniwati ◽  
Juni Handajani ◽  
Regina TC. Tandelilin
Keyword(s):  
Treatment Group ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Dental Plaque ◽  
Health Benefit ◽  
Green Tea Extract ◽  
Control Group ◽  
Research Subjects ◽  
Significant Difference ◽  
Tea Extract

Green tea (Camellia sinensis) has been widely known as a healthy drink since long time ago. One of the substances in green tea which can give health benefit is catechin, an antibacterial substance. The purpose of this research is to know the efficacy of rinsing with green tea extract to Streptococcus alpha growth on gingivitis patient’s dental plaque. The research subjects include 30 mild gingivitis patients, and these subjects are divided into two groups: treatment group (20 patients) and control group (10 patients). Ten patients of treatment group rinse their mouth with 0.25% green tea extract and 10 other patients with 0.5% green tea extract. Meanwhile, control group use Bactidol (0.1% Hexetidine). Rinsing the mouth is performed every morning and night for five days. Sampling is conducted on the first and sixth day. Before rinsing data is analyzed using ANOVA and the result shows a significant difference. ANAVA testing then is done using proportion value. ANAVA Testing result shows that there is no significant difference among the patients in the treatment group. This result indicates that the effect of rinsing with 0.25% and 0.5% green tea extract is equivalent to the control (0.1% Hexetidine) to inhibit S. alpha growth on mild gingivitis patient’s dental plaque. From these two concentrations, it has not been known which one is the most effective concentration to inhibit S. alpha growth on mild gingivitis patient.

scholarly journals Effect of camellia sinensis extract on the expression level of transcription factors and cytochrome p450 genes coding phase i drug-metabolizing enzymes

2013 ◽  
Vol 59 (4) ◽  
pp. 45-59
Author(s):  
Anna Bogacz ◽  
Monika Karasiewicz ◽  
Joanna Bartkowiak-Wieczorek ◽  
Marcin Ożarowski ◽  
Agnieszka Seremak-Mrozikiewicz ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Mrna Level ◽  
Green Tea Extract ◽  
Expression Level ◽  
Gene Transcript ◽  
Mrna Levels ◽  
Clinically Significant ◽  
Tea Extract

Abstract Green tea (Camellia sinensis) is widely used as a popular beverage and dietary supplement that can significantly reduce the risk of many diseases. Despite the widespread use of green tea, the data regarding the safety as well as herb-drug interactions are limited. Therefore, the aim of our study was to assess the influence of standardized green tea extract (GTE) containing 61% catechins and 0.1% caffeine on the expression level of rat CYP genes and the corresponding transcription factors expression by realtime PCR. The findings showed that GTE resulted in a significant decrease of CYP2C6 expression level by 68% (p<0.001). In case of CYP3A1 and CYP3A2, the mRNA levels were also reduced by extract but in a lesser degree compared to CYP2C6. Simultaneously the significant increase in the mRNA level of CAR, RXR and GR factors was observed by 54% (p<0.05), 79% (p<0.001) and 23% (p<0.05), respectively after 10 days of green tea extract administration. In addition, there was noted a small increase of CYP1A1 expression level by 21% (p>0.05) was noted. No statistically significant differences were observed for CYP1A2 and CYP2D1/2. In the same study we observed an increase in amount of ARNT gene transcript by 27% (p<0.05) in the long-term use. However, green tea extract showed the ability to stimulate HNF-1α both after 3 and 10 days of treatment by 30% (p<0.05) and 80% (p<0.001), respectively. In contrast, no change was observed in the concentration of HNF-4α cDNA. These results suggest that GTE may change the expression of CYP enzymes, especially CYP2C6 (homologue to human CYP2C9) and may participate in clinically significant interactions with drugs metabolized by these enzymes.

scholarly journals Investigation of Wound Healing Potential of Azadirachta indica Seed Extract using Wistar Rats

2020 ◽  
Vol 45 (6) ◽  
Author(s):  
I. A. Ajayi ◽  
O. B. Omolere
Keyword(s):  
Wound Healing ◽  
Azadirachta Indica ◽  
Wistar Rats ◽  
Wound Closure ◽  
Healing Process ◽  
Wound Healing Process ◽  
Wound Treatment ◽  
Phytochemical Screening ◽  
Wound Area ◽  
Seed Extracts

This study investigated the wound healing potential of hexane and methanolic seed extracts of Azadirachta indica using 35 wistar rats that were divided into 5 groups of 7 rats each. Phytochemical screening and antimicrobial activity of the extracts were carried out while the wound healing potential was evaluated by treating the test rats with 5 % and 10 % hexane and methanol extracts in an experiment that lasted for 21 days. Wound area and percentage of wound closure of the rats were noted at four-day intervals and at 21 days, the blood and organs of the rats were subjected to haematological and histopathological analyses respectively. The extracts were found to contain tannins, glycosides and phenols and they inhibited the growth of tested organisms. All the test rats displayed better and faster healing than the control ones but there were no significant differences between their haematological and histophatological results. The seed extracts quickened the wound healing process of the rats and might therefore be useful in wound treatment.

scholarly journals Esktrak ikan haruan (Channa striata) menurunkan jumlah makrofag pada fase inflamasi proses penyembuhan luka (Extract of haruan (Channa striata) decreases macrophages count in inflammation phase of wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 6
Author(s):  
Putri Sri Hartini ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Wistar Rats ◽  
Healing Process ◽  
Positive Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Post Test ◽  
Channa Striata

Haruan is a kind of freshwater fishes commonly found in South Kalimantan. Haruan extract contains nutritional substancessuch as albumin, Zn, Fe, Cu, and unsaturated fatty acid which can help accelerate wound healing. The aim of this studywas to histopathologically assess the effect of 100%, 50%, and 25%haruan extract compared to aquadest and ibuprofenon macrophages count in inflammation phase of wistar rats’ buccal mucosa wound healing. This research was a trueexperimental with post test-only with control design. Samples used were 30 wistar rats divided into 5 groups, 100%, 50%,25% haruan extract treatment groups, ibuprofen treatment group as positive control, and aquadest treatment group asnegative control. Mean macrophages counted on day 3 of 100%, 50%, 25% haruan extract treatment groups aquadest,and ibuprofenl were 2.05, 4.4, 3.9, 3.3, 2.4 respectively. In conclusion, haruan extract had a significant effect in decreasingmacrophages count in inflammation phase of wound healing.

scholarly journals PENGARUH PENGGUNAAN EKSTRAK DAUN TEH HIJAU (Camellia sinensis) PADA PROSES PEMBUATAN NAGET DAGING SAPI TERHADAP SIFAT KIMIA

2020 ◽  
Vol 1 (1) ◽  
pp. 34
Author(s):  
Muhammad Fakhri Maulana ◽  
Lilis Suryaningsih ◽  
Andry Pratama
Keyword(s):  
Moisture Content ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Design Method ◽  
Chemical Properties ◽  
Fat Content ◽  
Green Tea Extract ◽  
Polyphenol Content ◽  
Randomized Design ◽  
Tea Extract

Green tea is known as a plant who has many purpose as well as an antioxidant sources. The purpose of this research is to find out the effect of adding green tea (Camellia sinensis) extract in beef nuggets on chemical properties (moisture content, fat content, and polyphenol content). This research is an experimental research using completely randomized design method with 4 treatments and 5 repetition. The treatment that given was P1= beef nugget with no added green tea extract, P2= beef nugget + 0,5% green tea extract, P3= beef nugget + 1% green tea extract, and P4= beef nugget + 1,5% green tea extract with 5 repetition. Chemical analysis that used in this research is moisture content analysis, fat content, and polyphenols content. The obtained results then tested with anova, duncan, and polynomial test. The results showed that the treatment had a significantly real effect on polyphenols content but not on the moisture content and fat content. The addition of 1,5% green tea (Camellia sinensis) extract increase the polyphenol content of 305,96 mg/kg and influence 80,79% of the polyphenol content.

scholarly journals The effect of papaya leaf extract (Carica papaya L) on healing process of buccal traumatic ulcer in wistar rats

2019 ◽  
Vol 1 (1) ◽  
pp. 15
Author(s):  
Afryla Femilian ◽  
Dewi Agustina ◽  
Goeno Subagyo
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Buccal Mucosa ◽  
Wistar Rats ◽  
Carica Papaya ◽  
Healing Process ◽  
Herbal Medicines ◽  
Ethanolic Extract ◽  
Control Group ◽  
Wound Healing Process

Indonesians have been using herbal medicines for a long time to cure some illnesses. Carica papaya L is an example of an herb that contains papain enzymes, saponins, lysozymes, lipases, flavonoids, polyphenols and vitamin C. These  ingredients are believed to be beneficial for the wound healing process. The purpose of this study was to determine the effect of topical application of ethanolic extract of Carica papaya L to the healing process of rat mouth ulcer. Subjects were 32 Wistar rats divided into 2 groups (control group and treatment group), each group containing 16 rats. All subjects were made to suffer from ulcers using glacial acetyl acid applied for 40 seconds in the buccal mucosa. Treatment group was treated with papaya leaf ethanolic extract on ulcers twice a day using microbrush, while the ulcer in the control group was not treated with the extract. The ulcerated tissue was biopsied and stained with H&E. Observations were performed on the day 0, 3rd, 7th and 12th on HE slides. Data were observed by looking at three indicators of wound healing i.e. macrophage, angiogenesis and re-epithelisation. Number of macrophages and angiogenesis were analyzed using two-way ANOVA. Data of epithelial thickness were analyzed using Kruskal-Wallis test. The post hoc test in the treatment group and the control group on day 0 showed results of p = 1.00. On day 3, day 7 and day 12, the result of p was <0.05. In the treatment group on day 0 compared to the treatment group on the 3rd, 7th, 12th day, the results were p<0.05. In the treatment group on the 3rd day compared to the 7th and 12th days the results were p>0.05. The treatment group on the 7 day compared to the treatment group on the 12 day had p>0.05. In the control group, on day 0 compared to day 3, the results of p>0.05, while the control group day 0 with day 7, 12 had a result of p<0.05. The control group on the 3rd day was compared with the 7th day. The 12th also had a result of p<0.05. The comparison between the 7 day control groups showed p of >0.05. These data suggest that the papaya leaf ethanolic extract could accelerate the healing of oral ulcer on the buccal mucosa of wistar rats.

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