PEMANFAATAN SERBUK KAYU JATI (Tectona grandis L.) YANG DIRENDAM DALAM AIR DINGIN SEBAGAI MEDIA TUMBUH JAMUR TIRAM (Pleurotus comunicipae)

PERENNIAL ◽  
2006 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
. Baharuddin ◽  
Muh. Taufik Arfah ◽  
. Syahidah
Keyword(s):  
Mycelial Growth ◽  
Cold Water ◽  
Tectona Grandis ◽  
Dry Weight ◽  
Good Growth ◽  
Fruiting Bodies ◽  
Control Measurement ◽  
Time Periods ◽  
Time Required ◽  
Growth Ability

The growth responds of Pleurotus comunicipae to the use of cold water-soaked teakwood sawdust as a growth medium was investigated. Teakwood sawdust was soaked in cold water during the time periods of 1, 3, 5 and 7 days) with ten replications. Unsoaked teakwood sawdust was also prepared and used as a control. Measurement variabels consisted of time required for a completely covering mycelial growth, number of fruitbody, and fungal mass (green and dry weight). Results showed that P. comunicipae placed on soaked teakwood sawdust medium had a better growth than control (without soaking) medium. The medium with increased soaking period produced a good growth ability of P.comunicipae. At teakwood sawdust soaked for 7 days, the average of mycelial growth to cover teakwood sawdust medium, number of fruiting bodies, green weight and dry weight at the first harvesting were 52.6 days, 8.6 units, 100.5 grams and 13.4 grams, respectively. Keywords: teakwoods sawdust, Pleurotus comunicipae, cold water soaking, growth ability

scholarly journals Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1610
Author(s):  
Branka Vinterhalter ◽  
Nevena Banjac ◽  
Dragan Vinterhalter ◽  
Dijana Krstić-Milošević
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Bubble Column ◽  
Growth Parameters ◽  
Growth Index ◽  
Dry Weight ◽  
Growth Conditions ◽  
Good Growth ◽  
Gentiana Dinarica ◽  
Hairy Root Clones

The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

Development and quantitative measurement of microsclerotia of Verticillium dahliae

1972 ◽  
Vol 50 (11) ◽  
pp. 2097-2102 ◽  
Author(s):  
R. Hall ◽  
H. Ly
Keyword(s):  
Verticillium Dahliae ◽  
Mycelial Growth ◽  
Quantitative Measurement ◽  
Dry Weight ◽  
Pure Cultures ◽  
Carbon Nitrogen Ratio ◽  
Low Glucose ◽  
Nitrogen Ratio ◽  
Total Dry Weight ◽  
Hyaline Material

The development of microsclerotia of Verticillium dahliae from a few swollen hyaline cells on a hypha to a multicellular, pigmented "mature" structure is described and illustrated. A method for quantitatively estimating the amount of pigmented microsclerotial material in pure cultures was developed to study quantitative relations between mycelial growth and production of microsclerotial material in media containing different concentrations of glucose. At low glucose concentrations (0.6 to 10 mg/ml) microsclerotial material continued to increase after total dry weight of the cultures had reached a maximum, suggesting conversion of hyaline to pigmented material. At high glucose concentrations (20 to 60 mg/ml) the patterns of increase in total dry weight, microsclerotial material, and hyaline material were similar over a 4-week incubation period. Maximum production of both pigmented and hyaline materials occurred at a glucose concentration of 30 mg/ml (carbon/nitrogen ratio of 50/1).

scholarly journals Control of lettuce bottom rot by isolates of Trichoderma spp

2014 ◽  
Vol 40 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Zayame Vegette Pinto ◽  
Matheus Aparecido Pereira Cipriano ◽  
Amaury da Silva dos Santos ◽  
Ludwig Heinrich Pfenning ◽  
Flávia Rodrigues Alves Patrício
Keyword(s):  
Mycelial Growth ◽  
High Capacity ◽  
Dry Weight ◽  
Second Step ◽  
Trichoderma Spp ◽  
In Vivo Experiments ◽  
Bottom Rot ◽  
Trichoderma Isolates

Bottom rot, caused by Rhizoctonia solani AG 1-IB, is an important disease affecting lettuce in Brazil, where its biological control with Trichoderma was not developed yet. The present study was carried out with the aim of selecting Trichoderma isolates to be used in the control of lettuce bottom rot. Forty-six Trichoderma isolates, obtained with baits containing mycelia of the pathogen, were evaluated in experiments carried out in vitro and in vivo in a greenhouse in two steps. In the laboratory, the isolates were evaluated for their capabilities of parasitizing and producing toxic metabolic substances that could inhibit the pathogen mycelial growth. In the first step of the in vivo experiments, the number and the dry weight of lettuce seedlings of the cultivar White Boston were evaluated. In the second step, 12 isolates that were efficient in the first step and showed rapid growth and abundant sporulation in the laboratory were tested for their capability of controlling bottom rot in two repeated experiments, and had their species identified. The majority of the isolates of Trichoderma spp. (76%) showed high capacity for parasitism and 50% of them produced toxic metabolites capable of inhibiting 60-100% of R. solani AG1-IB mycelial growth. Twenty-four isolates increased the number and 23 isolates increased the dry weight of lettuce seedlings inoculated with the pathogen in the first step of the in vivo experiments.In both experiments of the second step, two isolates of T. virens, IBLF 04 and IBLF 50, reduced the severity of bottom rot and increased the number and the dry weight of lettuce seedlings inoculated with R. solani AG1-IB. These isolates had shown a high capacity for parasitism and production of toxic metabolic substances, indicating that the in vitro and in vivo steps employed in the present study were efficient in selecting antagonists to be used for the control of lettuce bottom rot.

scholarly journals Maintenance culture medium and inoculum based on peach palm leaves for Pleurotus spp. production

2016 ◽  
Vol 83 (0) ◽  
Author(s):  
Elisabeth Wisbeck ◽  
Endi Pricila Alves ◽  
Styfanie Gonçalves de Lima ◽  
Regina Maria Miranda Gern ◽  
Marcia Luciane Lange Silveira ◽  
...  
Keyword(s):  
Radial Velocity ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Agricultural Waste ◽  
Production Costs ◽  
Fruiting Bodies ◽  
Wheat Grains ◽  
Peach Palm ◽  
Maintenance Medium ◽  
Production Parameters

ABSTRACT: This study evaluated the use of immersion water from peach palm leaves (PPLDA) as a component of the culture medium for the maintenance of Pleurotus spp. and the use of agricultural waste, peach palm leaves, as inoculum support for the fungi. The performance of the inoculum based on peach palm leaves (PPL) for the production of Pleurotus spp. fruiting bodies was compared with that using wheat grains (WG) as inoculum support. PPLDA culture medium (immersion water of peach palm leaves, dextrose, and agar) showed a lower radial velocity of mycelial growth for both fungi than that obtained with the culture medium WDA (wheat extract, dextrose and agar), commonly used as maintenance medium for Pleurotus spp. However, the type of inoculum support does not significantly influence the linear velocity of P. ostreatus mycelial growth, reaching 6.71 mm/day on wheat grains and 6.18 mm/day on peach palm leaves. Thus, when the inoculum based on peach palm leaves is utilized, the immersion water used for preparing this support can be used for preparing the PPLDA maintenance culture medium, diminishing the production costs of Pleurotus mushrooms. Data also showed that when Pleurotus sajor-caju was cultivated on peach palm leaves, using PPL as inoculum support, the fruiting bodies production parameters (Y = 47%, BE = 3% and Pr = 0.2 g/day) did not differ from that obtained using WG.

Recent Growth, Biochemical Composition and Prey Field of Larval Haddock (Melanogrammus aeglefinus) and Atlantic Cod (Gadus morhua) on Georges Bank

1987 ◽  
Vol 44 (1) ◽  
pp. 14-25 ◽  
Author(s):  
L. J. Buckley ◽  
R. G. Lough
Keyword(s):  
Gadus Morhua ◽  
Atlantic Cod ◽  
Dry Weight ◽  
Melanogrammus Aeglefinus ◽  
Good Growth ◽  
Georges Bank ◽  
Larval Population ◽  
Prey Biomass ◽  
Recent Growth ◽  
Haddock Melanogrammus Aeglefinus

A transect across southern Georges Bank in May 1983 showed higher levels of available prey for haddock (Melanogrammus aeglefinus) and cod (Gadus morhua) larvae at two stratified sites than at a well-mixed site. At the stratified sites, prey biomass was high (30–300 μg dry wt∙L−1) near the surface above the thermocline; values were lower and more uniform with depth (10–30 μg dry wt∙L−1) at the well-mixed site. Larval population centers generally coincided with prey biomass vertically. Recent growth in dry weight of haddock larvae as estimated by RNA–DNA ratio analysis was higher at the stratified sites (8–13%∙d−1) than at the well-mixed site (7%∙d−1). Larvae appeared to be in excellent condition at the stratified sites, but up to 50% of haddock larvae from the well-mixed site had RNA–DNA ratios in the range observed for starved larvae in the laboratory. Cod collected at the same site were in better condition and growing faster than haddock. The data support the hypotheses that (1) stratified conditions in the spring favor good growth and survival of haddock larvae and (2) cod larvae are better adapted to grow and survive in well-mixed waters at lower levels of available food than haddock larvae.

scholarly journals Effects of pH and Aeration on Sclerotium rolfsii sacc. Mycelial Growth, Sclerotial Production and Germination

2018 ◽  
Vol 7 (3) ◽  
pp. 123-129 ◽  
Author(s):  
Fakher Ayed ◽  
Hayfa Jabnoun-Khiareddine ◽  
Rania Aydi-Ben-Abdallah ◽  
Mejda Daami-Remadi
Keyword(s):  
Mycelial Growth ◽  
Sclerotium Rolfsii ◽  
Dry Weight ◽  
Ph Values ◽  
Effects Of Ph ◽  
Ph Range ◽  
Sclerotial Development ◽  
Sclerotial Germination

Sclerotium rolfsii is one of the devastating soilborne fungus responsible for significant plant losses. The effects of pH and aeration on pathogen mycelial growth, sclerotial production and germination were investigated for three Tunisian isolates. Optimal mycelial growth occurred at pH 6 for Sr2 and Sr3 isolates and at pH 6-7 for Sr1. Dry mycelial growth was optimum at pH values ranging between 4 and 7. Sclerotial initiation started on the 3rd day of incubation at all pH values tested and mature sclerotia were formed after 6 to 12 days. Optimal sclerotial production was noted at pH 5. The dry weight of 100 sclerotia varied depending on isolates and pH and occurred at pH range 4-7. At pH 9, mycelial growth, sclerotial production and dry weight of 100 sclerotia were restricted. The optimum sclerotial germination, noted after 24 h of incubation, varied depending on isolates and pH and occurred at pH 4-9. Mycelial growth was optimum in aerated plates with a significant isolates x aeration treatments interaction. Sclerotial initiation occurred at the 3rd day of incubation and mature sclerotia were observed after 6-9 days. Sclerotial development was very slow in completely sealed plates and dark sclerotia were produced only after 15 days of incubation. The highest sclerotial yields were noted in aerated plates. The highest dry weight of 100 sclerotia for Sr1 isolate was recorded in ½ sealed, no sealed and completely sealed plates, while for Sr2, it was noted in ½ and ⅔ sealed plates. For Sr3, the maximum dry weight of 100 sclerotia was recorded in ½, ⅔ and completely sealed plates. Germination of S. rolfsii sclerotia, after 24 h of incubation, did not vary significantly depending on aeration treatments and ranged from 90 to 100% for all isolates.

scholarly journals The Effect of Tillage Treatments on Soil Temperature at Planting and on Corn (Zea mays L.) Yield

2001 ◽  
pp. 40-44
Author(s):  
Miklós Pakurár ◽  
László Lakatos ◽  
János Nagy
Keyword(s):  
Zea Mays ◽  
Soil Temperature ◽  
Zea Mays L ◽  
Time Period ◽  
Soil Temperatures ◽  
Time Periods ◽  
Time Required ◽  
Corn Hybrid

The effect of soil temperature was evaluated on the yield of the Occitan corn hybrid at a depth of 5 cm. We examined this effect on the time required from planting to emergence for three average durations: five, ten and fifteen days, all calculated from the day of planting. Winter plowing (27 cm), spring plowing (23 cm), disc-till (12 cm) treatments and 120 kg N per hectare fertilizer were applied. As a result of our analysis, we determined the post planting optimum soil temperatures for various time periods. The average soil temperature for a time period of 15 days post planting is the most usable for determining actual yields, followed by ten days, with five days proved to be the least usable (winter plow R2 = 0.86, spring plow R2 = 0.87, disc-till R2 = 0.64).

scholarly journals Ecology, Phylogeny, and Potential Nutritional and Medicinal Value of a Rare White “Maitake” Collected in a Mediterranean Forest

Diversity ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 230 ◽  
Author(s):  
Maria Letizia Gargano ◽  
Georgios I. Zervakis ◽  
Omoanghe S. Isikhuemhen ◽  
Giuseppe Venturella ◽  
Roberta Calvo ◽  
...  
Keyword(s):  
Cold Water ◽  
Water Extract ◽  
Its Region ◽  
Dry Weight ◽  
Field Investigation ◽  
Nutritional Composition ◽  
Nuclear Ribosomal Dna ◽  
Composition Analysis ◽  
Scientific Publications ◽  
Living Tree

Albino Grifola frondosa (Dicks.) Gray “maitake” mushrooms (described as G. albicans Imazeki and then placed in synonymy with G. frondosa) are particularly rare, and the few pertinent records are not treated in scientific publications. A field investigation carried out in Sicily (Italy) led to the collection of an unusual white Grifola specimen at the base of a living tree of Quercus pubescens Willd. s.l. The outcome of sequencing the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) indicated that it belongs to G. frondosa and provided an insight to the phylogenetic relationships within the genus. The results of nutritional composition analysis showed that the albino basidioma possesses relatively high contents of Ca, Fe, K, and Cu and is rather low in Na when compared with literature data on edible mushrooms. Vitamin (B1, B2, B3, B5, B9, and D2) contents ranged from 0.15 to 3.89 mg per 100 g of mushroom dry weight. The cold-water extract of this specimen was effective at inhibiting the growth of Staphylococcus epidermidis ATCC 12228 and Pseudomonas aeruginosa ATCC 15442 at the maximum screening concentration of 50% v/v. In addition, the extract slowed down the ability of Staphylococcus aureus ATCC 43300 to form biofilms. According to data hereby reported, the albino G. frondosa is a culinary-medicinal mushroom with a promising exploitation potential.

scholarly journals Effect of photosensitisers on growth and morphology of Phytophthora citrophthora coupled with leaf bioassays in pear seedlings

2020 ◽  
Vol 56 (No. 2) ◽  
pp. 74-82 ◽  
Author(s):  
Antonios Zambounis ◽  
Oksana Sytar ◽  
Dimitris Valasiadis ◽  
Zoe Hilioti
Keyword(s):  
Mycelial Growth ◽  
Biological Effects ◽  
Dry Weight ◽  
Colony Growth ◽  
Inhibitory Effect ◽  
Economic Losses ◽  
Inhibitory Effects ◽  
Phytophthora Citrophthora ◽  
Irregular Shapes ◽  
Zoospore Production

The phytopathogenic oomycetes of the genus Phytophthora cause devastating economic losses worldwide.<br />Naphthodianthrone compounds, present in plant extracts of buckwheat and Saint John’s wort act as photosensitiser<br />agents and exhibit antimicrobial activity against a number of pathogens. In this study, we investigated the potential<br />inhibitory effects of fagopyrin and hypericin on Phytophthora citrophthora (R.E. Sm. &amp; E.H. Sm.) Leonian 1906, the<br />main causal agent of rot diseases in deciduous trees. Fagopyrin had the highest inhibitory effect in the colony growth<br />at a concentration of 2% of a stock solution (3 mg/mL), inducing clubbed hyphae with round tips. Notably, hypericin<br />also inhibited the radial colony growth and increased the hyphal branching at the subapical region, while also promoting<br />the formation of enlarged cells with irregular shapes growing collectively as biofilm-like structures. In terms of the<br />mycelial dry weight, although both photosensitisers had considerable inhibitory effects, the fagopyrin treatment was<br />most effective. Leaf bioassays showed that under dark conditions the photosensitiser pre-treated zoospores formed a<br />dense, but aberrant, mycelial growth with penetration defects. In contrast, when the zoospore production was performed<br />under light conditions, the zoospores failed to cause necrotic lesions and penetration events implying that their<br />virulence was impaired. These findings shed light on the biological effects of fagopyrin and hypericin in the regulation<br />of the mycelial growth, morphology and pathogenicity of P. citrophthora.

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