scholarly journals BAKTERI AEROB DAN UJI KEPEKAAN ANTIMIKROBA

2016 ◽  
Vol 19 (1) ◽  
pp. 5
Author(s):  
Erviani Zuhriah ◽  
Nurhayana Sennang ◽  
Darmawaty ER
Keyword(s):  
Nosocomial Infection ◽  
Disease Transmission ◽  
Pathogenic Bacteria ◽  
Acinetobacter Calcoaceticus ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Antimicrobial Sensitivity ◽  
Air Sampler ◽  
Increased Risk ◽  
Sensitivity Pattern

Nosocomial infection is an infection acquired in hospitals, which occur more frequently in poor and developing countries such as Indonesia, one percent of the related cases leads to death. The occurrence of nosocomial infection causes the lengthening of hospitalization and increased risk of disease transmission. According to Permenkes No. 1204/Menkes/SK/X/2004, the Emergency Room is one of the high-risk areas. The objectives of this research were to quantify the number of aerobic bacteria, to know the identification method of the pathogenic bacteria and to determine the antimicrobial sensitivity pattern in the emergency installation. A cross sectional study was performed by collecting air samples at eleven treatment rooms of the Emergency installation in dr. Wahidin Sudirohusodo Hospital Makassar by using Microbiology Air Sampler 100 (MAS 100). The bacterial identification and antimicrobial susceptibility tests (AST) were conducted at the Balai Besar Laboratorium Kesehatan Makassar (July to September 2009). The number of bacterial colonies were 288–6570 CFU/m3. Pathogenic bacteria identified in the study were Stomatococcus mucilaginous, Staphylococcus haemolyticus and Acinetobacter calcoaceticus, which were still sensitive to tetracycline and resistant to ceftazidim and kanamycin.The number of bacterial colonies exceeded the standard number established by the Decree of Indonesian Health Minister

scholarly journals BAKTERI AEROB PATOGEN DAN UJI KEPEKAAN ANTIMIKROBA DI RUANGAN PERAWATAN PENYAKIT DALAM

2016 ◽  
Vol 18 (3) ◽  
pp. 168
Author(s):  
Fedelia Raya ◽  
Nurhayana Sennang ◽  
Suci Aprianti
Keyword(s):  
Internal Medicine ◽  
Nosocomial Infection ◽  
Pathogenic Bacteria ◽  
Acinetobacter Calcoaceticus ◽  
Cross Sectional Study ◽  
Internal Medicine Ward ◽  
Cross Sectional ◽  
Airborne Infection ◽  
Air Sampler ◽  
Hospital Patients

Pathogenic bacteria are the major causes of airborne infection at the hospital ward. Nosocomial infection can occur at the opened as well as at the closed room. Nosocomial infection influences the morbidity and mortality in the hospital and need an extra attention, because of the increased number of hospital patients, micro organism mutation and increased of bacteria resistance to antibiotics. The aim of this study was to quantify the number of aerobic bacteria, and to know the pathogenic bacteria identification and its determination on the susceptibility of the antimicrobial problems at the internal medicine ward. This research was carried by a cross sectional study, which performed by collecting air samples in eight internal medicine ward of Dr. Wahidin Sudirohusodo Hospital using Microbiology Air Sampler 100 (MAS 100). The bacterial identification and the antimicrobial susceptibility test (AST) were conducted at the Balai Besar Laboratorium Kesehatan (July to August 2009). In this study were found the numbers of bacteria colonies about 580–6040 CFU/m3. The pathogenic bacteria that identified were Acinetobacter calcoaceticus, Staphylococcus saprohpyticus, Enterobacter hafniae and Stomatococcus mucilaginosus that were sensitive to Amikasin, Gentamicyn, Azitromycin and Norfloxacyn but resistant to Ampicillin. The number of bacterial colonies exceeded the established number standard by Decree of the Indonesian Health Minister. The pathogenic bacteria showed the most sensitive result of AST were Acinetobacter calcoaceticus, Enterobacter hafniae, Stomatococcus mucilaginosus and Staphylococcus saprohpyticus.

scholarly journals BAKTERI PATOGEN AEROB DAN UJI KEPEKAANNYA DI RUANGAN BEDAH PUSAT

2018 ◽  
Vol 20 (2) ◽  
pp. 93
Author(s):  
Agustini Agustini ◽  
Nurhayana Sennang ◽  
Benny Rusli
Keyword(s):  
Disease Transmission ◽  
Pathogenic Bacteria ◽  
Alcaligenes Faecalis ◽  
Operating Rooms ◽  
Cross Sectional Study ◽  
Transmission Risk ◽  
Risk Area ◽  
Cross Sectional ◽  
Air Sampler

An operating room is potential in causing nosocomial infection in a hospital, especially due to surgical wound infections. Nosocomialinfection raises the length of day care and disease transmission risk. According to Permenkes No. 1204/Menkes/SK/X/2004, the operatingroom is a highly infection risk area. The aim of this study is to know the determination of the amount of bacterial colonies count, andthe sensitivity identification pattern of pathogenic bacteria in the central operating rooms at the Dr Wahidin Sudirohusodo Hospital.A cross sectional study was done by taking air samples using a Microbiology Air Sampler 100 in six central operating rooms in Dr.Wahidin Sudirohusodo Hospital. Counting bacterial colonies, identifying pathogenic bacteria and sensitivity test were done at the BalaiBesar Laboratorium Kesehatan Makassar (from May to June 2010). The result of the study shows that the amount of bacterial coloniesfound were approximately 148–440 CFU/m3 aerobic pathogenic bacteria consisting of Alcaligenes faecalis and Serratia liquefacienswhich were sensitive to sulbactam+ cefoperazone, gentamycin, levofloxacin but resistant to ampicillin, nitrofurantoin, clindamycin andmetronidazole. The researchers concluded that the amount of bacterial colonies found exceeded the Permenkes standard. The pathogenicbacteria were sensitive to sulbactam+ cefoperazone, gentamycin and levofloxacin however, resistant to ampicillin, nitrofurantoin,clindamycin, and metronidazole.

scholarly journals Bacteriological Spectrum and Antimicrobial Sensitivity of Pathogens In Children with Hematologic Malignancies and Febrile Neutropenia Research

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Anahita Sanaei Dashti ◽  
Jabarali Safaei ◽  
Fariba Shirvani ◽  
Mohammad Rahim Kadivar ◽  
Erfan Taherifard
Keyword(s):  
Febrile Neutropenia ◽  
Hematologic Malignancies ◽  
Cross Sectional Study ◽  
Blood Cultures ◽  
Coagulase Negative Staphylococci ◽  
Cross Sectional ◽  
Gram Negative ◽  
Antimicrobial Sensitivity ◽  
Demographic Questionnaire ◽  
Sensitivity Pattern

Objectives: This study aimed to investigate the spectrum of bacterial pathogens and their antimicrobial sensitivity in pediatric patients admitted with febrile neutropenia (FN) and hematologic malignancies. Methods: This prospective, cross-sectional study was carried out on 65 children (60% boys, mean age 7.3 5.3 years) with hematologic malignancies and FN hospitalized at the Amir Hematology and Oncology Hospital, Shiraz, Iran, between December 2018 and May 2019. Samples were taken from blood, nose, axilla, and inguinal area (NAI). NAI cultures were repeated after 72 hours of admission, and blood culture was repeated if needed. A demographic questionnaire on age, sex, and admission time was filled out for each patient. Results: The most common grown bacteria in the NAI samples were coagulase-negative staphylococci (CoNS). Second cultures from these sites revealed nosocomial contamination. Eighteen patients (27.7%) had positive blood cultures, with 61.1 % of the isolated pathogens being Gram-negative (mostly Escherichia coli and Pseudomonas aeroginosa) and 38.9% Gram-positive. The blood cultures results did not match those of the NAI cultures in 61.1% of the patients. About one-fourth of the Gram-negative pathogens isolated from blood were resistant to cefepime, the main empirical antibiotic for FN management at our center. Conclusions: Gram-negative bacteria are predominant organisms in pediatric FN patients at our center, most resistant to cefepime. If a similar antimicrobial sensitivity pattern is observed in future studies, a change in local guidelines might be considered for FN management.

scholarly journals Antimicrobial Sensitivity Pattern of Helicobacter pylori Isolates among Subgroup of Bangladeshi Patients

2014 ◽  
Vol 8 (2) ◽  
pp. 49-52
Author(s):  
MMSU Islam ◽  
Shamsun Nahar ◽  
Mst Naznin Sarker ◽  
ASM Salimullah ◽  
Mohammad Asadur Rahman ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Diarrhoeal Disease ◽  
Cross Sectional Study ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Cross Sectional ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
H Pylori ◽  
Medical University

This cross sectional study was carried out at Bangabandhu Sheikh Mujib Medical University (BSMMU) and International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B) from July 2008 to September 2009. Aim of the study was to find out the antimicrobial susceptibility profile of Helicobacter pylori isolates from dyspeptic patients. Total 224 dyspeptic patients from Out Patient Department (OPD) of BSMMU were initially enrolled after informed written consent. After upper GI endoscopy 157 patients were finally included who had erosions, ulcers or atrophic changes in the stomach or duodenum. Two biopsy samples were taken from each of them. Samples were incubated at 37°C in a double gas incubator with 5%O2, 10%CO2 and 85%N2. Total 82 (52.23%) samples were found positive for H. pylori. Isolated organisms were then tested for sensitivity to Amoxicillin, Clarithromycin, Tetracycline, Levofloxacin and Metronidazole by Agar dilution method. Among 82 patients 51(62.2%) were male and 31(37.8) were female with a male:female ratio 1.6:1. Patients were categorized into two groups one having gastric or duodenal ulcer (30.5%) and other having no ulcer (69.5%). Among these isolates 92.7% were sensitive to Amoxicillin, 89% to Clarithromycin, 81.7% to Tetracycline, 80.5% to Levofloxacin and only 26.8% to Metronidazole. Beside these, 81.7% isolates were sensitive to both Amoxicillin and Clarithromycin, 74.4% to Amoxicillin and Tetracycline, 73.2% to Amoxicillin and Levofloxacin, 72% to Clarithromycin and Tetracycline, 59% to Clarithromycin and Levofloxacin and 51% to Tetracycline and Levofloxacin DOI: http://dx.doi.org/10.3329/fmcj.v8i2.20280 Faridpur Med. Coll. J. 2013;8(2): 49-52

Study of bacterial infection and antimicrobial sensitivity pattern in diabetic foot lesion

2012 ◽  
Vol 6 (2) ◽  
pp. 20-23
Author(s):  
Shameem Akhter ◽  
Shah Md Zahurul Haque Asna ◽  
M Mushfequr Rahman ◽  
MA Hafez
Keyword(s):  
Diabetic Foot ◽  
Cross Sectional Study ◽  
Microbial Pathogens ◽  
Cross Sectional ◽  
Female Ratio ◽  
Antimicrobial Susceptibility Pattern ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Foot Lesions ◽  
Diabetic Foot Lesions

A cross sectional study was carried out on patients with diabetic foot lesions to determine the spectrum of aerobic microbial flora and to determine the microbial pathogens of the diabetic foot lesions and their antimicrobial sensitivity pattern. A total of 226 organisms were isolated from 218 patients and polymicrobial infection was found in 3.7 % cases. Age of the study population ranged from 20 - >80 yrs of which most of the patients were from 40 to 70 yrs (81 %). Male female ratio was= 3:1. In this study, Pseudomonas sp. (22.1 %), Proteus mirabilis (16.4 %) and S. aureus (14.6 %) were the predominant organisms isolated. Antimicrobial susceptibility pattern of the isolates were done in which imipenem and ciprofloxacin were found to be the most effective against all organisms.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19372 Bangladesh J Med Microbiol 2012; 06(02): 20-23

scholarly journals Methicillin-Resistant Staphylococcus aureus (MRSA): Prevalence and Antimicrobial Sensitivity Pattern among Patients—A Multicenter Study in Asmara, Eritrea

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Eyob Yohaness Garoy ◽  
Yacob Berhane Gebreab ◽  
Oliver Okoth Achila ◽  
Daniel Goitom Tekeste ◽  
Robel Kesete ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Public Health Problem ◽  
Cross Sectional Study ◽  
Resistance Pattern ◽  
Multiple Drug ◽  
Cross Sectional ◽  
Methicillin Resistant ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Referral Hospitals

Background. Methicillin-resistant Staphylococcus aureus (MRSA) is a well-recognized public health problem throughout the world. The evolution of new genetically distinct community-acquired and livestock-acquired MRSA and extended resistance to other non-β-lactams including vancomycin has only amplified the crisis. This paper presents data on the prevalence of MRSA and resistance pattern to other antibiotics on the selected specimen from two referral hospitals in Asmara, Eritrea. Method. A cross-sectional study was conducted among 130 participants recruited from two referral hospitals in Asmara, Eritrea. Isolation of S. aureus was based on culture and biochemical profiles. Standard antimicrobial disks representing multiple drug classes were subsequently set for oxacillin, gentamicin, erythromycin, and vancomycin. Data were analyzed using SPSS version 20 software. Results. S. aureus isolation rate from the 130 samples was 82 (63.1%). Patients <18 years of age were more likely to be colonized by S. aureus compared to patients above 61 years. The proportion of MRSA among the isolates was 59 (72%), methicillin-intermediate S. aureus (MISA) was 7 (8.5%), and methicillin-sensitive S. aureus (MSSA) was 15 (19.5%). The isolates were mostly from the pus specimen in burn, diabetic, and surgical wound patients. Antimicrobial susceptibility test showed that 13 (15.9%) of the isolates were resistant to vancomycin, 9 (11.0%) to erythromycin, and 1 (1.2%) to gentamicin. Coresistance of MRSA isolates to some commonly used antibiotics was also noted: oxacillin/erythromycin 5 (6.1%) and oxacillin/vancomycin 9 (11%). A few isolates were resistant to oxacillin/vancomycin/erythromycin 2 (2.4%) and oxacillin/gentamicin and erythromycin 1 (1.2%). Conclusion. This study reports a relatively high prevalence of MRSA. Isolates that are resistant to other tested antibiotics including vancomycin are also reported. The data have important implication for quality of patients care in the two settings: antibiotic selection and infection control practices, and the need for additional studies.

scholarly journals Bacterial Isolates from Blind Tracheal Aspirates and Their Anti-Microbial Sensitivity: An Observational Study in ICU of a Tertiary Care Hospital in Bangladesh

2020 ◽  
Vol 8 (2) ◽  
pp. 81-85
Author(s):  
Uzzwal Kumar Mallick ◽  
Md Sirajul Islam ◽  
Mohammad Asaduzzaman ◽  
Mohammad Abdullah Yusuf ◽  
Mohammad Omar Faruq
Keyword(s):  
Observational Study ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Sampling Technique ◽  
Cross Sectional Study ◽  
Care Hospital ◽  
Coagulase Negative Staphylococci ◽  
Cross Sectional ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

Background: The number of organisms developing resistance to commonly used antibiotics is increasing day by day. The exact national scenario of antimicrobial sensitivity pattern is not well known in Bangladesh owing to the absence of proper guideline for prescribing antibiotics. Aims: The aim of this study is to identify the group of organisms developing resistanceso that antibiotic policy can be formulated for the proper and effective use of antibiotics. Settings and Design: This observational study was conducted for a period of 1 year from January 2018 to December 2018 in a tertiary care hospital in Bangladesh. Materials and methods: This descriptive cross-sectional study was conducted in the Department of Microbiology, National Institute of Neurosciences and Hospital, Dhaka, Bangladesh from January 2018 to December 2018, using the convenient sampling technique. Tracheal secretions from patients in the intensive care unit (ICU), tested in Department of Microbiology, National Institute of Neurosciences and Hospital, were included in the study. The culture was done on blood and MacConkey agar and the sensitivity pattern was performed on Muller Hinton agar. Data were analyzed using SPSS version 23.0. Results: Out of the microorganisms isolated from positive growth cultures, Acinetobacter (57.8%) was the most common isolate followed by Klebsiella (22.9%). Acinetobacter, pseudomonas and klebsiella had good sensitivity to colistin(87.80%, 82.40% and 77.80% respectively), where as they showed less sensitivity to higher generationcephalosporin, penicillin and aminoglycosides. Conclusion: The commonest organism which was isolated from the endotracheal aspirate cultures were Acinetobacter, followed by Pseudomonas and Klebsiella and antimicrobial susceptibility testing showed sensitivity to Colistin, Tazobactam/piperacillin, Meropenem and aztreonam. Whereas coagulase negative staphylococci was isolated only in minority cases with highest sensitivity to vancomycin and linezolid. Bangladesh Crit Care J September 2020; 8(2): 81-85

scholarly journals OTITIS MEDIA;

2019 ◽  
Vol 26 (02) ◽  
Author(s):  
S.M Abbas Naqvi ◽  
Rubina Yaseen ◽  
Zulfiqar Ali Naqvi
Keyword(s):  
Otitis Media ◽  
Cross Sectional Study ◽  
Chronic Suppurative Otitis Media ◽  
Cross Sectional ◽  
Suppurative Otitis Media ◽  
Media Study ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Causative Agents ◽  
Medical University

… Background and Objectives: The objective of this study is to determine pattern of bacterial isolates and their antimicrobial sensitivity pattern among patients with chronic suppurative otitis media. Study Design: Prospective study (descriptive cross-sectional study). Setting: Department of ENT and Microbiology, Nishtar Medical University and Hospital Multan. Period: June 2014 to December 2014. Material and Methods: A total of 50 patients presenting with chronic suppurative otitis media having unilateral or bilateral ear discharge were enrolled from OPD of ENT department of Nishtar Hospital. Results: Of these 50 patients with CSOM, 22 (44%) were male patients while 28 (56%) were female patients. Mean age of our study cases was noted to be 12.52 ± 11.53 years (ranging from 5 years to 52 years). Most burden of the disease was seen in children i.e. 25 (50%) of the patients were less than 10 years of age. During the whole study unilateral discharge was seen and table 1 shows 50 specimens of CSOM were investigated for bacterial infestation and found to be infected with different bacteria. the highest number of cases were infected with s. aureus 24 (48 %) followed by 11 pseudomonas aeruginosa (22 %), 11 proteus mirabilis (22%) and 3 E. coli (6%) patients. Conclusions: Due to variation in climate, community, accessibility of medical care and prescription of antibiotics, the pattern vary in CSOM patients. Therefore it is very important to identify the causative agents of CSOM and their sensitivity pattern against various antibiotics before treatment of the disease in the patients.

scholarly journals SARS-CoV-2 seroprevalence and asymptomatic viral carriage in healthcare workers: a cross-sectional study

Thorax ◽  
2020 ◽  
Vol 75 (12) ◽  
pp. 1089-1094 ◽  
Author(s):  
Adrian Shields ◽  
Sian E Faustini ◽  
Marisol Perez-Toledo ◽  
Sian Jossi ◽  
Erin Aldera ◽  
...  
Keyword(s):  
Intensive Care ◽  
Healthcare Workers ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Nasopharyngeal Swab ◽  
Sectional Study ◽  
University Hospitals ◽  
Cross Sectional ◽  
General Internal ◽  
Increased Risk

ObjectiveTo determine the rates of asymptomatic viral carriage and seroprevalence of SARS-CoV-2 antibodies in healthcare workers.DesignA cross-sectional study of asymptomatic healthcare workers undertaken on 24/25 April 2020.SettingUniversity Hospitals Birmingham NHS Foundation Trust (UHBFT), UK.Participants545 asymptomatic healthcare workers were recruited while at work. Participants were invited to participate via the UHBFT social media. Exclusion criteria included current symptoms consistent with COVID-19. No potential participants were excluded.InterventionParticipants volunteered a nasopharyngeal swab and a venous blood sample that were tested for SARS-CoV-2 RNA and anti-SARS-CoV-2 spike glycoprotein antibodies, respectively. Results were interpreted in the context of prior illnesses and the hospital departments in which participants worked.Main outcome measureProportion of participants demonstrating infection and positive SARS-CoV-2 serology.ResultsThe point prevalence of SARS-CoV-2 viral carriage was 2.4% (n=13/545). The overall seroprevalence of SARS-CoV-2 antibodies was 24.4% (n=126/516). Participants who reported prior symptomatic illness had higher seroprevalence (37.5% vs 17.1%, χ2=21.1034, p<0.0001) and quantitatively greater antibody responses than those who had remained asymptomatic. Seroprevalence was greatest among those working in housekeeping (34.5%), acute medicine (33.3%) and general internal medicine (30.3%), with lower rates observed in participants working in intensive care (14.8%). BAME (Black, Asian and minority ethnic) ethnicity was associated with a significantly increased risk of seropositivity (OR: 1.92, 95% CI 1.14 to 3.23, p=0.01). Working on the intensive care unit was associated with a significantly lower risk of seropositivity compared with working in other areas of the hospital (OR: 0.28, 95% CI 0.09 to 0.78, p=0.02).Conclusions and relevanceWe identify differences in the occupational risk of exposure to SARS-CoV-2 between hospital departments and confirm asymptomatic seroconversion occurs in healthcare workers. Further investigation of these observations is required to inform future infection control and occupational health practices.

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