scholarly journals KOMPLEMEN SERUM C3C DAN LIMFOSIT T-CD4+ DARAH

2016 ◽  
Vol 19 (3) ◽  
pp. 197
Author(s):  
I. Komang Parwata ◽  
Endang Retnowati ◽  
Betty Agustina Tambunan
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Serum Levels ◽  
Absolute Number ◽  
Hiv And Aids ◽  
Stage I ◽  
Hiv Diagnosis ◽  
Becton Dickinson ◽  
Cross Sectional ◽  
Hiv Test

The incidence of HIV and AIDS infection continues to increase despite various treatments have been applied, thus the mortality rate remains high. The examination of CD4+ T lymphocytes number to determine the immune status and the monitoring of therapy has some limitations in facilities and personnel examination as well as expensive costs. The decrease in CD4+ T lymphocytes number will be followed by an increase in the virus number and complement activation, so that the C3c complement levels will decrease. The purpose of this study was to know the correlation between C3c complement serum levels and CD4+ T lymphocytes number in stage I HIV-infected patients by determining them. This research is an observational cross-sectional study. Thirty samples of stage I HIV-infected patients at the UPIPI of Dr. Soetomo Hospital were included in this study; they were collected between July and August 2011. HIV diagnosis was confirmed by positive HIV test results using three different methods. The CD4+ T lymphocytes number were examined using flowcytometry (FACS Calibur, Becton Dickinson (BD) Diagnostics) and complement C3c using Radial Immunodiffusion (NOR Partigen * C3c, Siemens). The results of complement C3c serum levels and CD4 + T lymphocytes number were analyzed with Pearson’s correlation and regression test (Pearson Product Moment Correlation) and Spearman’s Correlation test. The majority (83.33%) of C3c complement levels in stage I HIV-infected patients was still within normal limits (0.55 g/L up to 2.01 g/L; mean 1.39 g/L, SD 0.313 g/L) while the majority of CD4+ T lymphocytes absolute number (80%) were decreased (24-567 cells/μL; mean 295 cells/μL, SD 177 cells/μL). Based on a percentage value of CD4+ T lymphocytes, the majority (86.67%) decreased (2.54-29.48%; mean 13.58%, SD 6.7%). In this study was found that no significant correlation exists between C3c complement and CD4+ T lymphocyte absolute number with p=0.130 and percentage with p=0.217. There was no significant correlation of C3c complement and CD4+ T lymphocyte. This means that C3c complement examination can not be used to predict CD4+ T lymphocytes number.

scholarly journals KADAR TGF-β1 PLASMA DAN LIMFOSIT-T CD4+DI PENDERITA YANG TERINFEKSI HIV STADIUM I

2018 ◽  
Vol 20 (2) ◽  
pp. 122
Author(s):  
Alberthina Alberthina ◽  
Endang R ◽  
Erwin AT
Keyword(s):  
T Lymphocytes ◽  
Hiv Infection ◽  
Plasma Levels ◽  
Absolute Number ◽  
Hiv And Aids ◽  
Stage I ◽  
Cross Sectional ◽  
Number Of Patients ◽  
The Absolute ◽  
Tgf Β1

HIV infection and AIDS have been spread throughout the world and the number of patients continues to increase from year to year.Indonesia is one of the countries with quite a high increase in the incidence of HIV and AIDS. The absolute number of CD4+T-lymphocytesand percentage in HIV-infected patients can be used to determine the stage of the disease, and progression of the disease, as well as topredict the onset of the opportunistic diseases. In certain circumstances sometimes it is difficult to determine clinically and the results ofthe absolute number of CD4+ T-lymphocytes and its percentage are still high. So the examination of TGF-β1 is necessary for predictingthe disease course in the patient, because the increase of the disease progress is also accompanied by the increased levels of TGF-β1. Thepurpose of this study is to know and to prove the existence of the correlation between plasma levels of TGF-β1 and the absolute numberpercentage of CD4+T-lymphocytes in stage I HIV-infected patients. The research was carried out by a Cross sectional observational study,the samples were derived from 41 stage I HIV-infected patients treated at the Outpatient Clinic of the Infectious Disease IntermediateCare Unit (UPIPI) in the Dr. Soetomo Hospital from January to May 2012. The examination of TGF-β1 plasma was performed by ELISAmethod, the number of absolute and percentage of CD4+T-lymphocyte were counted by immuno flowcytometry (BD FACSCalibur™). Theresults were statistically analyzed using a Pearson product moment correlation test. It was shown that the TGF-β1 plasma levels in stageI HIV-infected patients tended to increase, as well as the number and percentage of CD4+ T-lymphocytes which were also increased. Theresult of this study revealed that the number of CD4+T-lymphocytes which were less absolute and more than 200 cells /μL showed nocorrelation with the plasma levels of TGF-β1 in stage I HIV infected patients. However, there was a significant positive correlation betweenthe number of CD4+T-lymphocytes percentage with TGF-β1 plasma in stage I HIV infection.

PROTEIN 24 HIV DAN LIMFOSIT T-CD4+ DI INFEKSI HIV TAHAP I

2016 ◽  
Vol 21 (3) ◽  
pp. 273
Author(s):  
I Made Sila Darmana ◽  
Endang Retnowati ◽  
Erwin Astha Triyono
Keyword(s):  
T Lymphocytes ◽  
Hiv Infection ◽  
T Lymphocyte ◽  
Negative Correlation ◽  
Stage I ◽  
Cross Sectional ◽  
Protein Levels ◽  
P24 Protein ◽  
Persistent Generalized Lymphadenopathy ◽  
Spearman Test

Measuring HIV p24 protein is a test which is more practical than determination of CD4+ T-lymphocyte counts and viral load, as it does not require a very sophisticated instrument and requires a lower cost. Independent predictive value of p24 to the decline of CD4+ T-lymphocytes, clinical progression and survival in HIV-infected patients have been reported. In this study, HIV-infected patients were found to have HIV p24 protein levels inversely proportional to CD4+ T-lymphocyte counts by using Spearman test (R2=0.225; p=0.0331). Studies on the correlation between HIV p24 protein levels and CD4+ T-lymphocyte counts in stage I HIV infection have not yet been reported. The aim of this study was to prove the correlation between HIV p24 protein levels and CD4+ T-lymphocytes in stage I HIV infection. Research issue was whether a correlation between HIV p24 protein levels and CD4+ T-lymphocyte counts in stage I HIVinfection existed ? The hypothesis was that a correlation between HIV p24 protein levels and CD4+ T-lymphocyte counts in stage I HIV infection existed. The study design was cross sectional observational. Subjects consisted of 30 stage I HIV-infected patients treated at the Infectious Disease Intermediate Care Unit, Dr. Soetomo Hospital and VCT Clinic of the Dr. Ramelan Naval Hospital, Surabaya from May to July 2014. Stage I HIV infection is an asymptomatic HIV infection or with persistent generalized lymphadenopathy and the patient is able to perform normal activities. Levels of p24 were measured by ELISA method and CD4+ T-lymphocyte counts using flowcytometry(BD FACSCaliburTM). The results were statistically analyzed using Pearson’s correlation test. HIV p24 protein levels in stage I of HIV infection ranged from 1.8 to 10.8 pg/mL, mean of 5.14 pg/mL and a standard deviation of 2.08 pg/mL. CD4+ T-lymphocyte counts decreased with a range of 49-559 cells /uL for absolute values and 4.42–26.02% for percentage values Correlations between blood p24 levels and CD4+ T-lymphocyte counts either absolute (r=–0.392, p=0.032) or percentage (r=–0.363, p=0.049) were found. In stage I HIV-infected patients, a negative correlation was found between p24 levels and CD4+ T-lymphocyte counts, in both CD4+T-lymphocyte counts as absolute and as well as percentage values. This negative correlation showed that the p24 HIV levels were inversely proportional to the CD4+ T-lymphocyte counts. HIV p24 protein levels have a possibility to be used predicting CD4+ T-lymphocyte counts

scholarly journals PROTEIN 24 HIV DAN LIMFOSIT T-CD4+ DI INFEKSI HIV TAHAP I (HIV p24 Protein and CD4+ T-lymphocyte in Stage I HIV infection)

2018 ◽  
Vol 21 (3) ◽  
pp. 273
Author(s):  
I Made Sila Darmana ◽  
Endang Retnowati ◽  
Erwin Astha Triyono
Keyword(s):  
T Lymphocytes ◽  
Hiv Infection ◽  
T Lymphocyte ◽  
Negative Correlation ◽  
Stage I ◽  
Cross Sectional ◽  
Protein Levels ◽  
P24 Protein ◽  
Persistent Generalized Lymphadenopathy ◽  
Spearman Test

Measuring HIV p24 protein is a test which is more practical than determination of CD4+ T-lymphocyte counts and viral load, asit does not require a very sophisticated instrument and requires a lower cost. Independent predictive value of p24 to the decline ofCD4+ T-lymphocytes, clinical progression and survival in HIV-infected patients have been reported. In this study, HIV-infected patientswere found to have HIV p24 protein levels inversely proportional to CD4+ T-lymphocyte counts by using Spearman test (R2=0.225;p=0.0331). Studies on the correlation between HIV p24 protein levels and CD4+ T-lymphocyte counts in stage I HIV infection have notyet been reported. The aim of this study was to prove the correlation between HIV p24 protein levels and CD4+ T-lymphocytes in stageI HIV infection. Research issue was whether a correlation between HIV p24 protein levels and CD4+ T-lymphocyte counts in stage I HIVinfection existed ? The hypothesis was that a correlation between HIV p24 protein levels and CD4+ T-lymphocyte counts in stage I HIVinfection existed. The study design was cross sectional observational. Subjects consisted of 30 stage I HIV-infected patients treated at theInfectious Disease Intermediate Care Unit, Dr. Soetomo Hospital and VCT Clinic of the Dr. Ramelan Naval Hospital, Surabaya from Mayto July 2014. Stage I HIV infection is an asymptomatic HIV infection or with persistent generalized lymphadenopathy and the patientis able to perform normal activities. Levels of p24 were measured by ELISA method and CD4+ T-lymphocyte counts using flowcytometry(BD FACSCaliburTM). The results were statistically analyzed using Pearson’s correlation test. HIV p24 protein levels in stage I of HIVinfection ranged from 1.8 to 10.8 pg/mL, mean of 5.14 pg/mL and a standard deviation of 2.08 pg/mL. CD4+ T-lymphocyte countsdecreased with a range of 49-559 cells /uL for absolute values and 4.42–26.02% for percentage values Correlations between blood p24levels and CD4+ T-lymphocyte counts either absolute (r=–0.392, p=0.032) or percentage (r=–0.363, p=0.049) were found. In stageI HIV-infected patients, a negative correlation was found between p24 levels and CD4+ T-lymphocyte counts, in both CD4+T-lymphocytecounts as absolute and as well as percentage values. This negative correlation showed that the p24 HIV levels were inversely proportionalto the CD4+ T-lymphocyte counts. HIV p24 protein levels have a possibility to be used predicting CD4+ T-lymphocyte counts.

scholarly journals Dynamics of minor T-lymphocyte subpopulations in patients undergoing kidney transplantation

2020 ◽  
pp. 75-83
Author(s):  
S. V. Zybleva ◽  
S. L. Zyblev
Keyword(s):  
Kidney Transplantation ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Graft Function ◽  
Absolute Number ◽  
Primary Graft Dysfunction ◽  
Graft Dysfunction ◽  
Double Positive ◽  
Significant Difference ◽  
The Absolute

Objective: to study the dynamics of the indicators of CD3+CD4+CD8+double-positive and CD3+CD4-CD8- double-negative T-lymphocytes in patients who underwent kidney transplantation. Material and methods. Three groups were formed out of 197 allograft recipients. PGF group consisted of patients with primary satisfactory graft function. PGD group - with primary graft dysfunction. GR group - with primary graft dysfunction and histologically confirmed graft rejection. We studied the CD3+CD4+CD8+ (DP) and CD3+CD4-CD8- (DN) T-lymphocyte levels before the transplantation, and on the 1st, 3rd, 7th, 30th, and 90th days after the transplantation. Results. Within a month after the transplantation we noted a decrease in the relative DN T-lymphocyte level in the PGF group, while in the PGD and GR groups this indicator significantly increased. By the 90th day, the count of DN T-lymphocytes had remained unchanged in the PGD group, while there had been a statistically significant increase of this subpopulation in the PGF group. The absolute counts of DN T-lymphocytes in the PGF group on the 1st and 7th days were lower than in the GR group. On the 90th day, there was no statistically significant difference in the absolute number of DN T-lymphocytes in the recipient groups. In all the groups, there was a decrease in the number of DP T-lymphocytes on the 1st day, however, in the PGF group, the relative level was significantly higher. This tendency retained for 3 months. There were no statistically significant differences between the PGD and GR groups. The absolute number of DP T-lymphocytes in the PGF group during the entire observation period was significantly higher than in the PGD and GR groups. Conclusion. We noted a decrease in the indicators of DN T-lymphocytes in the PGF group associated with the increase in the DP T-lymphocyte level within the first three months. In the PGD and GR groups, an increase in the DN T-lymphocyte level was revealed due to a decrease in the indicators of DP T-lymphocytes within 90 days after transplantation.

scholarly journals Effect of Inhibited T lymphocyte Function on Depressive Symptoms in Breast Cancer Patients with Depression

2021 ◽  
Author(s):  
Jun-He Zhou ◽  
Wei-Han Li ◽  
De-Long Zhang ◽  
Bai-Le Ning ◽  
Lin Zhao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Quality Of Life ◽  
T Lymphocytes ◽  
Cancer Patients ◽  
T Lymphocyte ◽  
Structural Equations ◽  
Lymphocyte Function ◽  
Breast Cancer Patients ◽  
Cross Sectional

Abstract Background: Depression has a high incidence among patients with breast cancer, but the relationship between depression and cancer-related physiological changes is not clear.Objectives: To explore the effect of T lymphocytes on breast cancer depression and the patient’s quality of life.Methods: This is a cross-sectional study. A total of 93 breast cancer patients with depression were recruited, 46 of whom underwent T lymphocyte, cortisol, BDNF, TNF-α, and IL-1β collection. We analysed the correlation between the indicators in these 46 participants and constructed two intermediary structural equations between their T lymphocytes and depression, as well as their T lymphocytes and their quality of life.Results: The results showed that CD4+ had a positive correlation with BDNF (r=0.334, P=0.023) and that BDNF had a negative correlation with HAMD-24 (r=-0.390, P=0.007). Both CD3+ and CD8+ cells were negatively correlated with cortisol (r=-0.358, P=0.015, r=-0.411, P=0.005), and cortisol was positively correlated with FACT-B (r=0.435, P=0.003). The equations including CD4+, BDNF, and HAMD-24, as well as the equations including CD3+, CD8+, cortisol, and FACT-B, were established. BDNF was the mediating variable between CD4+ and HAMD-24. Cortisol was the mediating variable between CD3+, CD8+ and FACT-B. Neither HAMD-24 nor FACT-B could form a direct path with T lymphocytes.Conclusion: T lymphocytes may be involved in the depression of breast cancer patients since a poor quality of life could inhibit T lymphocytes, and this may be the underlying physiological cause of breast cancer-related depression.

Impaired T-Lymphocyte Subpopulations and Deranged Profile of Immunologic Activation in Patients with Gaucher Disease Type I

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4915-4915
Author(s):  
Argiris S Symeonidis ◽  
George Theodorou ◽  
Constantina Repa ◽  
Theodore Marinakis ◽  
Panayiotis Tsaftaridis ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Gaucher Disease ◽  
Lymphocyte Activation ◽  
Absolute Number ◽  
Lymphocyte Subpopulations ◽  
Type I ◽  
Cd8 T Lymphocytes ◽  
T Lymphocyte Subpopulations ◽  
Hla Dr

Abstract Patients with Gaucher disease exhibit substantial evidence of impairment of their immune system, namely, increased serum levels of proinflammatory cytokines and immunoglobulins, and increased incidence of B-cell malignancies, such as non-Hodgkin’s lymphoma, MGUS and multiple myeloma. We investigated peripheral blood T-lymphocyte subpopulations with dual color flow cytometry, as well as the direction of T-lymphocyte activation, by using intracytoplasmic immunostaining for IL-2, IL-4, IL-10 and IFN-gamma, on resting CD4+ and CD8+ T-lymphocytes and following activation with PMA- 1 with the presence of Brefeldin-A. Evaluations were performed on 16 patients with type I Gaucher disease and on 17 healthy controls. Patients had significantly decreased absolute lymphocyte count (1621±684 vs 2148±566/mm3, p=0.013), CD3+ (1197±478 vs 1508±431/mm3, p=0.045) and CD4+ T-lymphocytes (658±245 vs 945±253/mm3, p=0.021), but not CD8+ T-lymphocytes (491±331 vs 486±189/mm3, p: n.s.), resulting in a significant reduction of the CD4/CD8 ratio (1.59±0.68 vs 2.16±0.83, p=0.041). The populations of naive CD4+CD45RA+ and of memory CD4+CD45RO+ T-lymphocytes were also significantly decreased (218±128 vs 432±179/mm3, p=0.0005 and 484±185 vs 631±231/mm3, p=0.056 respectively), however, CD8+CD45RA+ and CD8+CD45RO+ subpopulations did nor differ significantly, when compared to controls. CD3−CD56+, but not CD3+CD56+ lymphocytes were also decreased (131±82 vs 199±97/mm3, p=0.037). Patients had higher percentages of CD8+ (29.2±9.7 vs 23.5±6.8%, p=0.042), CD8+CD45RA+ (22.1±6.2 vs 18.3±5.0%, p=0.046) and CD8+CD45RO+ T-lymphocytes (13.2±6.2 vs 9.6±3.7%, p=0.027), as well as of activated CD8+HLA-DR+ (0.93±0.68 vs 0.48±0.21%, p=0.008) and CD4+HLA-DR+ T-lymphocytes (1.77±0.93 vs 1.09±0.48%, p=0.008). Moreover, although both, the absolute number and the percentage of CD20+ B-lymphocytes were similar, patients exhibited significantly increased absolute number and percentage of CD5+CD20+ B-lymphocytes (1.63±0.55 vs 0.64±0.37% p=0.00002 and 29±20 vs 13±8/mm3, p=0.011, respectively). Finally, patients with Gaucher disease had significantly increased resting TH2-polarized CD4+T-lymphocytes (CD4+IL-10+: 0.41±0.29 vs 0.24±0.11%, p=0.045) and TH1-polarized CD8+ T-lymphocytes (CD8+IFNγ+: 0.15±0.07 vs 0.08±0.04%, p=0.005, CD8+IL10+: 0.22±0.08 vs 0.32±0.014, p=0.052, and IFNγ+/IL4+ ratio among the CD8+ population 2.54±2.1 vs 1.08±0.91, p=0.018). Following mitogenic activation a very significant impairment of obtaining the TH1 phenotype was observed (CD4+IL2+ lymphocytes 33.7±17.1 vs 65.4±6.1%, p<0.00001). The above findings suggest that in patients with Gaucher disease there is a significant numerical impairment of T-helper lymphocytes and a shift towards TH-2 direction of lymphocyte activation. These findings may explain the rarity of autoimmune manifestations despite the chronic inflammatory reaction, as well as the increased incidence of lymphoid malignancies, which has been reported among patients suffering from this disease.

scholarly journals Establishment of Normal Reference Intervals for CD3+, CD4+, CD8+, and CD4+to CD8+Ratio of T Lymphocytes in HIV Negative Adults from University of Gondar Hospital, North West Ethiopia

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Addisu Gize ◽  
Biniam Mathewos ◽  
Beyene Moges ◽  
Meseret Workineh ◽  
Lealem Gedefaw
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Reference Interval ◽  
Reference Intervals ◽  
University Hospital ◽  
T Lymphocyte Subsets ◽  
Cross Sectional ◽  
North West ◽  
Normal Reference

Background.Reference values for the CD3+, CD4+, CD8+, and CD4+to CD8+ratio T lymphocyte subsets are adopted from textbooks. But for appropriate diagnosis, treatment, and follow-up of patients, correct interpretations of the laboratory results from normal reference interval are mandatory. This study was, therefore, planned to establish normal reference interval for T lymphocytes subset count and CD4+to CD8+ratio.Methods.A cross-sectional study was conducted on apparently healthy adult individuals who visited voluntary counseling and HIV testing clinic Gondar University Hospital from April to May, 2013. Whole blood was analyzed using fluorescence-activated cell sorting (BD FACS, San Jose, CA) machine to enumerate the T-cell subpopulations.Results.Out of the total 320 study participants, 161 (50.3%) were men and 159 (49.7%) were women. The normal reference intervals were (655–2,823 cells/μL), (321–1,389 cells/μL), and (220–1,664 cells/μL) for CD3+, CD4+, and CD8+T lymphocyte subsets, respectively, and CD4+to CD8+ratio was 0.5–2.5.Conclusion.The overall CD3+T lymphocytes reference interval in the current study was wide; low CD4+T lymphocytes, CD4 to CD8 ratio, and high CD8+T lymphocytes values were observed.

scholarly journals Perilaku Tes HIV pada Laki-Laki yang Berhubungan Seks dengan Laki-Laki (LSL) di Provinsi Bali

2016 ◽  
Vol 11 (2) ◽  
pp. 47 ◽  
Author(s):  
Putu Krisna Saputra ◽  
Bagoes Widjanarko
Keyword(s):  
Hiv Testing ◽  
Univariate Analysis ◽  
Hiv And Aids ◽  
P Value ◽  
High Risk Population ◽  
Chi Square ◽  
Cross Sectional ◽  
Voluntary Counseling And Testing ◽  
Hiv Test ◽  
History Of

ABSTRACTMen who have sex with men (MSM) is a high risk population in infected with HIV and AIDS and Sexually Transmitted Infections (STIs) . Based on data from the Integrated Biological and Behavioral Surveillance (IBBS) in 2011 found that HIV prevalence among MSM is between 2.4 % to 17 % . Based on data from the Gaya Dewata Foundation , the number of new MSM reached in 2013 amounted to 1,322 people . However , the number of HIV tests among MSM in 2013 only amounted to 874. While the number of new HIV cases among MSM in 2013 amounted to 118 cases , this number increased by two times in 2011, which is amounted to 50 cases. This research is a quantitative study with cross sectional approach. The number of respondents 110 people choosen by purposive sampling technique.Quantitative data collection techniques through interviews with the questionnaire. Data were analyzed using univariate analysis with frequency distribution, using the chi-square bivariate, and multivariate logistic regression. The results showed that the variables associated with HIV testing behavior among MSM in Bali Province is the type of place to stay and history of STIs . Multivariate analysis showed that a history of STIs ( p value = 0.005 ) is the variable that has the most significant effect on the behavior of HIV testing in MSM in Bali Province with OR of 3.400. HIV testing in MSM behavior quite well. The majority of respondents had an HIV test within 0-6 months ago. Most respondents did last HIV test in Denpasar. Forms of HIV tests performed by the respondent is entirely voluntary counseling and testing ( VCT ) as well as all respondents knew of his HIV test results.Keywords: MSM, HIV Testing, Bali

scholarly journals Performance evaluation of the novel portable PIMA point-of-care CD4 analyzer in comparison with conventional flow cytometer instruments in Cameroon

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Bertrand Sagnia ◽  
Rachel Kamgaing ◽  
Charles Kouanfack ◽  
Georgette Kamdem ◽  
Samuel Sosso ◽  
...  
Keyword(s):  
Health Care ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Point Of Care ◽  
Low Cost ◽  
Urban Environments ◽  
Resource Constrained ◽  
Becton Dickinson ◽  
Inform Consent ◽  
Rural And Urban

Abstract Background Absolute CD4+ T-lymphocyte counts are used in the initiation and monitoring of antiretroviral therapy in HIV-infected patients: with the increase number of HIV infected patient and the reduce number of heath care system in rural zones, HIV monitoring in resource-constrained settings demands affordable and reliable CD4+ T lymphocytes enumeration methods. We evaluated a simple PIMA POC which is a dedicated system for enumeration that uses immunomagnetic and immunofluorescent technologies. The instrument was designed to be a low-cost, yet reliable and robust one. In this study, we assessed the correlation between most representative flow cytometry instruments present in Cameroon instead of CyFlow from PARTEC, FACSCount, and FACSCalibur both from Becton Dickinson. Methods CD4 surface markers on lymphocytes was measured on samples collected in EDTA tubes from 268 patients aged from 1 to 65 years old in three different health care structures. HIV infected patients are coming from CIRCB, Day Hospital of Hopital Central de Yaounde (HCY) and Hopital General de Yaounde (HGY). After inform consent, samples were collected and 101 samples were tested with the FACSCalibur, 60 samples were tested with the CyFlow and 107 samples were tested with the FACSCount flow cytometers. All these samples were tested by different technician with PIMA POC present in all these health care structures and the correlation and agreement were analyzed using linear regression and Bland–Altman analysis. Results The PIMA POC system has excellent precision, accuracy and linearity for CD4+ T lymphocytes enumeration. Good correlations were obtained between the PIMA POC system and other single platform methods. Bland–Altman plots showed interchangeability between the three machines. Absolute CD4+ T-lymphocyte values obtained from the PIMA system correlated well with Cyflow, FACSCount, and FACSCalibur method (r2 varies from 0.88 to 0.968, P < 0.0001). The comparison between values obtained from PIMA with CYFLOW, FACSCount, and FACSCalibur give P = 0.17, P = 0.5 and P = 0.6 respectively meaning that there is not significant differences between values obtained with PIMA and other flow machines. Conclusion This POC PIMA system is a simple and reliable system for enumeration of absolute CD4+ T-lymphocytes. Having one PIMA system easy to use, should reduce the cost and thus increase access to CD4 testing for HIV infected patients in resource-constrained countries. POC CD4 may also alleviate testing burdens at traditional central CD4 laboratories, hence improving test access in both rural and urban environments. This will reduce also the loss of follow up.

scholarly journals FACTORS AFFECTING HOUSEWIVES’ ATTITUDES TO HIV AND AIDS TEST IN YOGYAKARTA, INDONESIA

2021 ◽  
Vol 21 (2) ◽  
pp. 434-439
Author(s):  
Nanik Setiyawati ◽  
Niken Meilani
Keyword(s):  
Hiv Testing ◽  
Program Analysis ◽  
Family Income ◽  
Analysis Data ◽  
Hiv And Aids ◽  
Community Leaders ◽  
Good Knowledge ◽  
Cross Sectional ◽  
Hiv Test ◽  
Hiv Aids

  HIV testing becomes one of the standard components of mother and child health and family planning services at every level of health care. Increase case oh HIV among Housewives. There are 67% of pregnant women supported HIV test, but only 24% who tested HIV. This study aims to know the factors that influence the housewife attitude toward HIV testing in Yogyakarta which includes knowledge, mother’s belief in HIV testing, belief the attitude of her husband, friend and community leaders. This research is correlational analytic with cross sectional design.  The sample are 350 housewives in Yogya City and Sleman district that already implemented Prevention Mother to Child Transmission for HIV program. Analysis data use chi square and logistic regression. The result is majority of respondents: mothers aged >35 years, secundipara, education level is middle, husband's work as an employee and family income above the regional minimum income standard. Majority respondents have a good knowledge of HIV/AIDS and HIV testing, supportive attitudes toward HIV testing and have high belief in HIV testing from husband's attitudes and community leader’s attitude. Respondents’ belief that the attitude of friends did not support HIV testing for them. The majority have a supportive attitude to HIV testing are have a good knowledge of HIV/AIDS, have high belief in HIV testing, have high belief in the attitudes of husbands, friends and community leaders. The most factors influence on mother's attitude toward HIV testing is mother belief in her husband's attitude

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