Essential nutrients and oxidative stress of durum wheat under water stress.

2014 ◽  
Vol 3 (1) ◽  
pp. 123-128
Author(s):  
Issaad Ghozlène ◽  
Djebar Mohammed-Réda ◽  
Berrebbah Houria ◽  
Charga Ali

Oxidative stress in plants is the subject of numerous reviews of the literature (Apel and Hirt, 2004; Noctor and Foyer, 2005a; Pitzschke et al, 2006;. Wormuth et al, 2007;. Sbartai et al, 2012.) And of several books (Inze and Montagu, 2001; Smirnoff et al, 2005.). This work focuses on studying the effects of water stress on the roots of a plant model: durum wheat (Triticum durum) variety GTA. After the germination, the plants were subjected to water stress during (03, 05, 07 and 09 days). Our results demonstrate abiochemical and metabolic disturbances of the seed subjected to water deficit. The lack of water has caused significant changes in the GTA durum variety.

2014 ◽  
Vol 71 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Hanna Bandurska ◽  
Jolanta Floryszak-Wieczorek

The purpose of this paper was to examine the effect of water deficit on membrane injuries, superoxide radical generation and lipid peroxidation in the leaves of two barley genotypes. Six-day-old seedlings of the cv. Aramir and line R567 were used in the experiments. According to our earlier work these genotypes significantly differ in the level of membrane injuries under water deficit conditions. Water stress was applied directly to leaves or to roots. The stress caused considerable membrane injuries in the leaves of all genotypes investigated.The percentage membrane injury was higher in the line R567 than in the cv. Aramir. Water stress imposed on leaves caused higher membrane injuries than water stress imposed on roots. The water stress treatment followed by an oxidative stress in the leaves. Line R567 having noticeably larger membrane injuries also exhibited a higher level of superoxide radical generation than the cv. Aramir. The level of lipid peroxidation increased in the both genotypes under the conditions of water stress imposed on leaves, but not on roots.


Toxics ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 55
Author(s):  
Nicholas Kurchaba ◽  
Bryan J. Cassone ◽  
Caleb Northam ◽  
Bernadette F. Ardelli ◽  
Christophe M. R. LeMoine

Plastic polymers have quickly become one of the most abundant materials on Earth due to their low production cost and high versatility. Unfortunately, some of the discarded plastic can make its way into the environment and become fragmented into smaller microscopic particles, termed secondary microplastics (MP). In addition, primary MP, purposely manufactured microscopic plastic particles, can also make their way into our environment via various routes. Owing to their size and resilience, these MP can then be easily ingested by living organisms. The effect of MP particles on living organisms is suspected to have negative implications, especially during early development. In this study, we examined the effects of polyethylene MP ingestion for four and ten days of exposure starting at 5 days post-fertilization (dpf). In particular, we examined the effects of polyethylene MP exposure on resting metabolic rate, on gene expression of several inflammatory and oxidative stress linked genes, and on microbiome composition between treatments. Overall, we found no evidence of broad metabolic disturbances or inflammatory markers in MP-exposed fish for either period of time. However, there was a significant increase in the oxidative stress mediator L-FABP that occurred at 15 dpf. Furthermore, the microbiome was disrupted by MP exposure, with evidence of an increased abundance of Bacteroidetes in MP fish, a combination frequently found in intestinal pathologies. Thus, it appears that acute polyethylene MP exposure can increase oxidative stress and dysbiosis, which may render the animal more susceptible to diseases.


2014 ◽  
Vol 92 (10) ◽  
pp. 827-837 ◽  
Author(s):  
Varun Saran ◽  
Vijay Sharma ◽  
Richard Wambolt ◽  
Violet G. Yuen ◽  
Michael Allard ◽  
...  

Metabolic disturbances and oxidative stress have been highlighted as potential causative factors for the development of diabetic cardiomyopathy. The β-blocker metoprolol is known to improve function in the diabetic rat heart and ameliorates the sequelae associated with oxidative stress, without lowering oxidative stress. The antioxidant ascorbic acid is known to improve function in the diabetic rat heart. We tested whether a combination of ascorbic acid and metoprolol treatment would improve function further than each drug individually. Control and streptozotocin-induced diabetic Wistar rats were treated with metoprolol (15 mg·(kg body mass)−1·day−1, via an osmotic pump) and (or) ascorbic acid (1000 mg·(kg body mass)−1·day−1, via their drinking water). To study the effect of treatment on the development of dysfunction, we examined time points before (5 weeks diabetic) and after (7 weeks diabetic) development of overt systolic dysfunction. Echocardiography and working-heart-perfusion were used to assess cardiac function. Blood and tissue samples were collected to assess the severity of disease and oxidative stress. While both drugs improved function, only ascorbic acid had effects on oxidative damage. Combination treatment had a more pronounced improvement in function. Our β-blocker + antioxidant treatment strategy focused on oxidative stress, not diabetes specifically; therefore, it may prove useful in other diseases where oxidative stress contributes to the pathology.


2012 ◽  
Vol 167 (3) ◽  
pp. 433-440 ◽  
Author(s):  
Tania Siahanidou ◽  
Maria Garatzioti ◽  
Christina Lazaropoulou ◽  
Georgia Kourlaba ◽  
Ioannis Papassotiriou ◽  
...  

Objectiveα-Klotho (α-KL), a protein with antiaging properties, regulates phosphate, calcium, and bone metabolism, induces resistance to oxidative stress, and may participate in insulin signaling. The role of α-KL in neonates, known to be prone to metabolic disturbances and oxidative stress, is not known. The aim of this study was to evaluate circulating soluble α-KL concentrations in preterm and full-term neonates and unravel possible correlations with growth, metabolism, and indices of oxidative stress.DesignProspective study.MethodsPlasma-soluble α-KL levels were determined by specific ELISA in 50 healthy neonates (25 preterm, mean (s.d.) gestational age (GA) 33.7 (1.1) weeks, and 25 full-term infants) at days 14 and 28 of life. Associations of α-KL with anthropometric, metabolic parameters, and indices of oxidative stress were examined.Resultsα-KL levels were significantly higher in full-term than in preterm infants at both days 14 (1099 (480) pg/ml vs 884 (239) pg/ml respectively; P<0.05) and 28 (1277 (444) pg/ml vs 983 (264) pg/ml respectively; P<0.01). In both preterm and full-term infants, α-KL levels increased significantly from day 14 to 28 of life (P<0.001). Circulating α-KL concentrations correlated with GA (β=0.32, P=0.001), body weight (β=0.34, P=0.001), body length (β=0.33, P=0.001), 1,25-dihydroxy-vitamin D level (β=0.24, P<0.05), and malondialdehyde level (β=0.20, P<0.05) but not with glucose, insulin, or homeostasis model assessment index of insulin resistance values.ConclusionsSoluble α-KL levels rise as GA and postnatal age advance in neonates and may have an impact on vitamin D metabolism and oxidative stress. Whether α-KL may have a role in the regulation of infants' growth should be further studied.


2020 ◽  
Vol 75 (1) ◽  
pp. 43-65
Author(s):  
JUSTYNA LEŚNIOWSKA-NOWAK ◽  
MAGDALENA SOZONIUK ◽  
MAGDALENA KAWĘCKA ◽  
KORNELIA MAGA ◽  
MAŁGORZATA MROZEK

Oxidative stress can significantly impair the plants growth and development. It can be triggered by various biotic and abiotic factors. The study analyzed durum wheat genotypes aiming at identifying the forms characterized by increased tolerance to stress induced by methyl viologen (paraquat). The presence of paraquat in the medium caused the majority of analyzed forms to reduce the weight and length of the shoot part of seedling. In addition, inhibition of the root system development compared to control forms was observed. In some of the forms studied, oxidative stress caused chlorosis. Six different types of responses to oxidative stress were found. Most genotypes (58.8%) showed a reduction in seedling weight and length, irrespective of the paraquat concentration used. Nine genotypes resistant to stressor (CYP, MEX × 2, ETH, FRA, ITA, POL, SUN, TUN) were identified, which constituted 6.1% of the examined forms.


2018 ◽  
Author(s):  
Stephen A. Banse ◽  
Benjamin W. Blue ◽  
Kristin J. Robinson ◽  
Cody M. Jarrett ◽  
Patrick C. Phillips

AbstractAn organism’s ability to mount a physiological response to external stressors is fundamental to its interaction with the environment. Experimental exploration of these interactions benefits greatly from the ability to maintain tight control of the environment, even under conditions in which it would be normal for the subject to flee the stressor. ere we present a nematode research platform that pairs automated image acquisition and analysis with a custom microfluidic device. This platform enables tight environmental control in low-density, single-worm arenas, which preclude animal escape while still allowing a broad range of behavioral activities. The platform is easily scalable, with two 50 arena arrays per chip and an imaging capacity of 600 animals per scanning device. Validating the device using dietary, osmotic, and oxidative stress indicates that it should be of broad use as a research platform, including eventual adaptation for additional stressors, anthelmintic-drug screening, and toxicology studies.


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