scholarly journals Reuse of Residual Biomass of Cellulose Industry for Second Generation Bioethanol Production

2016 ◽  
Vol 6 (1) ◽  
pp. 768-772
Author(s):  
Nei Pereira Junior ◽  
Anelize de Oliveira Moraes ◽  
Luiz Felipe Modesto ◽  
Ninoska Isabel Bojorge Ramirez
Keyword(s):  
Ethanol Production ◽  
First Generation ◽  
Pulp Mill ◽  
Pulp Mills ◽  
Residual Biomass ◽  
Commercial Strain ◽  
Second Generation Bioethanol ◽  
Ssf Process ◽  
Range Of Values ◽  
Simultaneous Saccharification

This study aimed at evaluating the potential of pulp mill residue (PMR) as a feedstock for ethanol production. The simultaneous saccharification and fermentation (SSF) process was operated using 8 gL -1 of a commercial strain of Saccharomyces cerevisiae JP1 under optimal proportions of cellulase cocktail (24.8 FPU/g cellulose of Cellic® CTec2) and cellulosic residue (200 gL -1 ). After 48 hours of pre-hydrolysis at 50ºC and 200 rpm, the fermentation was carried out at 37 ºC, generating 48.5 gL -1 of ethanol in 10 hours and reaching a conversion efficiency of 53.3% from cellulose to ethanol and a volumetric productivity of 4.8 gL -1 h -1 that is within the range of values of first generation ethanol production (5-8 gL -1 h -1 ). These results showed that the pulp mill residue is an interesting and effective feedstock for the production of ethanol, which can be used for fuel purposes in the own pulp mills.  

Expression of serpins by Clostridium thermocellum and simultaneous saccharification of lignocellulosic biomass to enhance ethanol production

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 8662-8676
Author(s):  
Maria Mushtaq ◽  
Muhammad Javaid Asad ◽  
Muhammad Zeeshan Hyder ◽  
Syed Muhammad Saqlan Naqvi ◽  
Saad Imran Malik ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Corn Stover ◽  
Rice Straw ◽  
Clostridium Thermocellum ◽  
Ethanol Yield ◽  
Biofuel Production ◽  
Yeast Fermentation ◽  
Second Generation Bioethanol ◽  
Simultaneous Saccharification

Utilization of biomass for production of second generation bioethanol was considered as a way to reduce burdens of fossil fuel in Pakistan. The materials wheat straw, rice straw, cotton stalk, corn stover, and peel wastes were used in this experiment. Various parameters, such as acidic and alkali pretreatment, enzymatic hydrolysis by cellulases, and effect of proteases inhibitors on ethanol production, were examined. Fermentation was completed by the yeasts Saccharomyces cerevisiae and Clostridium thermocellum separately, and their ethanol production were compared and maximum ethanol yield was obtained with wheat straw i.e.,11.3 g/L by S. cerevisiae and 8.5 g/L by C. thermocellum. Results indicated that a higher quantity of sugar was obtained from wheat straw (19.6 ± 1.6 g/L) followed by rice straw (17.6 ± 0.6 g/L) and corn stover (16.1 ± 0.9 g/L) compared to the other evaluated biomass samples. A higher yield of ethanol (11.3 g/L) was observed when a glucose concentration of 21.7 g/L was used, for which yeast fermentation efficiency was 92%. Results also revealed the increased in ethanol production (93%) by using celluases in combination with recombinant Serine protease inhibitors from C. thermocellum. It is expected that the use of recombinant serpins with cellulases will play a major role in the biofuel production by using agricultural biomass. This will also help in the economics of the biofuel.

Ethanol Production from Hydrothermal Pretreated Empty Fruit Bunches

2014 ◽  
Vol 917 ◽  
pp. 80-86
Author(s):  
Mohd Saman Siti Aisyah ◽  
Pacharakamol Petchpradab ◽  
Yoshimitsu Uemura ◽  
Suzana Yusup ◽  
Machi Kanna ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Lignocellulosic Biomass ◽  
Simultaneous Saccharification And Fermentation ◽  
Process Time ◽  
Empty Fruit Bunches ◽  
Separate Hydrolysis And Fermentation ◽  
Common Process ◽  
The Common ◽  
Ssf Process ◽  
Simultaneous Saccharification

Separate hydrolysis and fermentation (SHF) is the common process in producing ethanol from lignocellulosic biomass. Nowadays, simultaneous saccharification and fermentation (SSF) process has been seen as potential process for producing ethanol with shortens process time with higher yield of ethanol. Hence, in the current work, the utilization of empty fruit bunches (EFB) in SSF process was studied. In order to improve saccharification reactivity of EFB, hydrothermal pretreatment at 180 and 220 °C was used to pretreat EFB. The findings showed that SSF has the potential in producing ethanol from EFB.

scholarly journals Development opportunities of biomass-based ethanol production in relation to starch- and cellulosebased bioethanol production

2013 ◽  
pp. 71-75
Author(s):  
Zoltán Balla
Keyword(s):  
Ethanol Production ◽  
Life Cycle Analysis ◽  
Liquid Fuel ◽  
First Generation ◽  
Second Generation ◽  
Point Of View ◽  
Raw Material ◽  
Bioethanol Production ◽  
Fuel Production ◽  
Second Generation Bioethanol

The biomass is such a row material that is available in large quantities and it can be utilizied by the biotechnology in the future. Nowadays the technology which can process ligno cellulose and break down into fermentable sugars is being researched. One possible field of use of biomass is the liquid fuel production such as ethanol production. Based on the literary life cycle analysis, I compared the starch-based (first generation) to cellulose-based (second generation) bioethanol production in my study considering into account various environmental factors (land use, raw material production, energy balance). After my examination I came to the conclusion that the use of bioethanol, independent of its production technology, is favorable from environmental point of view but the application of second generation bioethanol has greater environmentally benefits.

Ethanol Production from Sugarcane Bagasse by Zymomonas mobilis Using Simultaneous Saccharification and Fermentation (SSF) Process

2009 ◽  
Vol 161 (1-8) ◽  
pp. 93-105 ◽  
Author(s):  
Danielle da Silveira dos Santos ◽  
Anna Carolina Camelo ◽  
Kelly Cristina Pedro Rodrigues ◽  
Luís Cláudio Carlos ◽  
Nei Pereira
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Zymomonas Mobilis ◽  
Simultaneous Saccharification And Fermentation ◽  
Ssf Process ◽  
Simultaneous Saccharification

scholarly journals Kinetic modeling of simultaneous saccharification and fermentation of corn starch for ethanol production.

2014 ◽  
Vol 61 (1) ◽  
Author(s):  
Wojciech Białas ◽  
Adrian Czerniak ◽  
Daria Szymanowska-Powałowska
Keyword(s):  
Central Composite Design ◽  
Ethanol Production ◽  
Corn Starch ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Kinetics Model ◽  
Ssf Process ◽  
Central Composite ◽  
Simultaneous Saccharification ◽  
Granular Starch

Fuel ethanol production, using a simultaneous saccharification and fermentation process (SSF) of native starch from corn flour, has been performed using Saccharomyces cerevisiae and a granular starch hydrolyzing enzyme. The quantitative effects of mash concentration, enzyme dose and pH were investigated with the use of a Box-Wilson central composite design protocol. Proceeding from results obtained in optimal fermentation conditions, a kinetics model relating the utilization rates of starch and glucose as well as the production rates of ethanol and biomass was tested. Moreover, scanning electron microscopy (SEM) was applied to investigate corn starch granule surface after the SFF process. A maximum ethanol concentration of 110.36 g/l was obtained for native corn starch using a mash concentration of 25%, which resulted in ethanol yield of 85.71%. The optimal conditions for the above yield were found with an enzyme dose of 2.05 ml/kg and pH of 5.0. These results indicate that by using a central composite design, it is possible to determine optimal values of the fermentation parameters for maximum ethanol production. The investigated kinetics model can be used to describe SSF process conducted with granular starch hydrolyzing enzymes. The SEM micrographs reveal randomly distributed holes on the surface of granules.

scholarly journals Production of alcohol by simultaneous saccharification and fermentation of low-grade wheat flour

2006 ◽  
Vol 49 (3) ◽  
pp. 481-490 ◽  
Author(s):  
Marcos Antonio das Neves ◽  
Toshinori Kimura ◽  
Naoto Shimizu ◽  
Kiwamu Shiiba
Keyword(s):  
Ethanol Production ◽  
Wheat Flour ◽  
Simultaneous Saccharification And Fermentation ◽  
Alpha Amylase ◽  
Fermentable Sugars ◽  
Low Grade ◽  
Atp Production ◽  
Two Samples ◽  
Ssf Process ◽  
Simultaneous Saccharification

Two samples of low-grade wheat flour, namely low-grade 1 (LG1) and low-grade 2 (LG2), with different carbohydrate and fibrous content, were used as substrates. The samples were liquefied using various concentrations of alpha- or beta-amylase, in order to optimize the production of fermentable sugars; the enzyme alpha-amylase revealed higher performance. After liquefaction, the simultaneous saccharification and fermentation was conducted in a jar fermentor. Amyloglucosidase was used for saccharification, and dry baker's yeast, S. cerevisiae, for fermentation simultaneously. Glucose was consumed promptly in both cases, LG1 and LG2; ethanol production was considerably higher in LG1 (38.6 g/L), compared to LG2 (24.9 g/L). The maximum ATP production was observed early in the SSF process. LG1 revealed higher potential as substrate for ethanol production.

scholarly journals HIGH SOLID FERMENTATION AT PILOT SCALE FOR POTABALE ETHANOL PRODUCTION FROM RICE

2018 ◽  
Vol 55 (5A) ◽  
pp. 178
Author(s):  
Nguyen Chinh Nghia
Keyword(s):  
Ethanol Production ◽  
Dry Matter ◽  
Rice Flour ◽  
Pilot Scale ◽  
Beta Glucanase ◽  
Theoretical Yield ◽  
Vhg Fermentation ◽  
Active Dry Yeast ◽  
Ssf Process ◽  
Simultaneous Saccharification

In recent year, besides conventional process comprising separated liquefaction, saccharification and fermentation steps, few ethanol distilleries in Vietnam started using Simultaneous Saccharification and Fermentation (SSF) for ethanol production. In this work, we developed an SSF process of rice mash at high solids for potable ethanol production at pilot scale of 30 liters. After investigation on liquefaction and boiling time of five SSF processes, we selected the most suitable one (SSF3) to apply to pilot scale. At pilot scale, rice flour (RF) was dissolved in water to reach 315.4 g/l dry matter, then the mixture was liquefied at 85 oC for 60 min by using an alpha-amylase (Liquozyme SC DS at 45.36 KNU-S/kg RF) and a beta-glucanase (Optimash TBG at 2.812 U/kg CF). The starch slurry was then boiled for 30 min. SSF of liquefied mash was performed at 30 oC with the simultaneous addition of a gluco-amylase (Spirizyme SC DS at 540 AGU/kg RF) and a protease (FermgenTM at 600 SAPU/kg RF), active dry yeast (Ethanol Red at 1.5×107cells/l), urea (12 mM) and KH2PO4 (4 mM). Under these conditions, SSF process finished after 120 h and achieved 16.7  % v/v ethanol, which was equivalent to 87.1  % of the theoretical yield. Therefore, the VHG fermentation of rice flour could be a great potential to be applied in potable ethanol industry in Vietnam.

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