scholarly journals Abamectin Induced Biochemical and Histopathological Changes in the Albino Rat, Rattus Norvegicus

2013 ◽  
Vol 53 (3) ◽  
pp. 263-270 ◽  
Author(s):  
Hany Kamal Abd-Elhady ◽  
Gamal Elsayed Abou-Elghar
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Corn Oil ◽  
Male Rats ◽  
Streptomyces Avermitilis ◽  
Sublethal Dose ◽  
Histopathological Changes ◽  
Albino Rat ◽  
Liver And Kidney ◽  
Sublethal Doses

Abstract Abamectin (Avermectin B1a), is a natural fermentation product derived from the soil bacterium Streptomyces avermitilis. Abamectin (Avermectin B1a) is widely used as an insecticide, acaricide, and anthelmintic. The present study assessed the effects of repeated subacute and subchronic exposure to the commercial formulation of abamectin (Vertemic, 1.8% EC) in albino male rats. The toxic effects of abamectin were studied. The various biochemical parameters and histopathological changes were noted. A stomach tube was used to orally administer sublethal doses of abamectin suspended in corn oil to the rats. The animals were divided into four groups. Rats of the group T1 were orally administered a sublethal dose of 30 mg/kg body weight (b.wt.) (1/10 LD50) three times a week for 30 days and the animals in group T2 were exposed to 10 mg/kg b.wt. (1/30 LD50) for 210 days, once a week. Two control groups (C1 and C2) were used in parallel studies, where animals were administered a corn oil vehicle. At the end of the study period, blood samples were collected from all groups to measure plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST) activities, and the levels of creatinine and urea. Also, total protein and RNA contents were determined in the liver and kidney tissues. Changes in biochemical parameters were more intense in male rats from group T2 than those reported in group T1. The levels of ALT, AST, urea and creatinine were significantly elevated in rats from group T2 when compared to the control. In group T2, a significant decrease in the levels of total protein and RNA in both the liver and kidneys was observed. Fertility was also significantly reduced in male rats ingesting abamectin in group T2. The number of offspring was significantly reduced. Histopathological changes were more intense in male rats from group T2 than those from group T1. In conclusion, the results of this study demonstrate that subchronic oral administration of abamectin altered some biochemical parameters which correlated with histopathological changes

scholarly journals Toxicity studies of sea anemone (Gyrostoma helianthus) crude extract and fractionated proteins on liver and kidney in rats

2021 ◽  
Vol 42 (1) ◽  
pp. 135-143
Author(s):  
Turki M. Al-Shaikh ◽  
Keyword(s):  
Crude Extract ◽  
Biochemical Parameters ◽  
Treatment Strategies ◽  
Sea Anemone ◽  
Protein Fractions ◽  
Male Rats ◽  
Toxicity Data ◽  
Histopathological Changes ◽  
Liver And Kidney ◽  
Glomerular Tuft

Aim: To study the poisonous effects of crude extract and fractionated protein of sea anemone Gyrostoma helianthus on histological and biochemical parameters in male rats. Methodology: Live specimens of Gyrostoma helianthus were collected from Jeddah coast, fixed in ethanol, dried and powdered to obtain the crude residue extract. Some of the crude extract were ultra-filtered to produce protein fractions of 1 and 3KDa. The LD50 of crude extract powder was estimated for male rats and momentary repeated subacute dosing (1/4 LD50) for 7 days was carried out to obtain toxicity data. Histological examination of liver and kidney sections were carried out. Serum whole protein, total albumin, ALT and LDH were estimated by standard protocol. Results: The LD50 of crude extract was 20.32 mg kg-1 for male rats. In acute and subsequent sub-acute toxicity, neurological symptoms such as convulsions, paralysis, tremors, and ataxia were observed overdose exposure. At the end of exposure to subacute dose histopathological changes like hemolyzed blood and atrophy of glomerular tuft in kidney and fatty changes, vacuolation, necrosis, and infiltration in liver was noted. Furthermore, vital significant increase in total protein, ALT and LDH and reduced bilirubin in serum of treated groups was observed as compared to the control. Interpretation: The present study emphasizes the toxicological, behavioral, biochemical, and histological bioactivity of crude extract and protein fractions of 1 and 3 KDa of Gyrostoma helianthus sea anemone, which is commonly found in the Red Sea. The tested extracts were found to be active at a concentration 5.08 mg kg-1. The yielded effects may interpret treatment strategies of toxicological and pharmacologic intervention. Key words: Biochemistry, Gyrostoma helianthus, Histopathology, Toxicity

Histopathological changes in the liver and kidney tissues of Wistar albino rat exposed to fenitrothion

2008 ◽  
Vol 24 (9) ◽  
pp. 581-586 ◽  
Author(s):  
S Afshar ◽  
AA Farshid ◽  
R Heidari ◽  
M Ilkhanipour
Keyword(s):  
Control Group ◽  
Histopathological Changes ◽  
Albino Rat ◽  
Hydropic Degeneration ◽  
Leukocytic Infiltration ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Dose Dependent ◽  
Significant Injury ◽  
Different Doses

The aim of this study was to investigate the dose-related effects of fenitrothion (FNT) on the liver and kidney. The study was conducted on 8-week-old male Wistar rats that were divided into four groups (three experimental groups and one control group) and were treated orally with different doses (25, 50, 100 mg/kg) of FNT for 28 consecutive days. After treatment, the rats were anesthetized with ether and liver and kidney samples were taken for histological studies. The results showed that the histopathological changes in the liver were mainly represented by parenchymatous degeneration of hepatocytes with mild necrosis, leukocytic infiltration in the portal area, severe congestion, and hemorrhage. These changes were dose dependent. Marked tubular dilation, hydropic degeneration in tubular epithelium, moderate congestion, and hemorrhage in the cortical and medulla part of the kidney were recorded. Histopathologic examination of the liver and kidney indicated a significant injury only in rats receiving 100 mg/kg FNT.

scholarly journals Effects of peptidic growth hormone secretagogue receptor (GHS-R) antagonist [D-Lys3] on some of serum hormonal and biochemical parameters in Wistar rat model

2014 ◽  
Vol 58 (3) ◽  
pp. 288-291 ◽  
Author(s):  
Habib Aghdam Shahryar ◽  
Alireza Lotfi
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Serum Lipids ◽  
Considerable Increase ◽  
Wistar Rat ◽  
Male Rats ◽  
Growth Hormone Secretagogue Receptor ◽  
Growth Hormone Secretagogue ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective : The present study investigated the effects of different dosages of a GHS-R antagonist [D-Lys3] on some serum hormonal (cortisol, T3 and T4) and biochemical parameters in a rat.Materials and methods : Thirty-six 60-day-old male rats were assigned to four treatments. [D-Lys3]-GHRP-6 solutions were infused via intraperitoneal injections. Blood was collected and analyzed.Results : The large dosages of a GHS-R antagonist (200 ng/kg BW) caused increases in cortisol, whereas no significant changes occurred when low dosages were injected. There were no significant changes in T3 and T4 following the administration of the GHS-R antagonist, but a considerable increase was observed in blood glucose levels of the groups (G50, G100, and G200 ng/kg BW). There was a significant increase in total protein when the greatest dose was administrated (G200 ng/kg BW). However, total cholesterol, triglycerides, and albumin showed no significant changes.Conclusions : Exogenous GHS-R antagonist can cause an increase in glucose and moderate increases in cortisol and total protein, yet it has no significant effect on T3 and T4 levels or on the concentrations of serum lipids. The effect of GHS-R antagonist is not completely adverse to the effects of ghrelin. Further molecular studies are necessary to identify the physiological effects of the peptidic GHS-R antagonist. Arq Bras Endocrinol Metab. 2014;58(3):288-91

scholarly journals Influence of combined treatment with mianserin and simvastatin on selected biochemical serum parameters of liver and kidney function in rats

2013 ◽  
Vol 26 (3) ◽  
pp. 263-266
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Day Treatment ◽  
Combined Treatment ◽  
Creatinine Concentration ◽  
Serum Parameters ◽  
Combined Administration ◽  
Liver And Kidney ◽  
The Impact

The aim of the present study was to assess the impact of combined 14-day treatment with mianserin (10 mg/kg) and simvastatin (1 or 10 mg/kg) on selected biochemical liver and kidney parameters in rats (AST and ALT activities and the concentrations of AFP, total protein, urea, creatinine and ß2-M). The results showed the increase in both transaminases activities, creatinine concentration and the decrease of AFP, total protein and ß2-M concentrations. The results indicate that 14-day combined administration of mianserin with simvastatin negatively affects the liver functioning. The observed changes in kidney biochemical parameters may suggest a risk of renal dysfunction during long-term combined treatment with these drugs.

scholarly journals The Pathological effct of Aeromonas caviae bacteria and clinical chemistry – In rats

2003 ◽  
Vol 27 (1) ◽  
pp. 61-77
Author(s):  
Bshra Ibrahem Al-Qaese
Keyword(s):  
Total Protein ◽  
Clinical Chemistry ◽  
White Male ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Male Rats ◽  
Clinical Samples ◽  
Histopathological Changes ◽  
Blood Picture ◽  
Aeromonas Caviae

The study aimed at Investigating the effect of Aeromonas caviae baceria which cause Systemic and Local infection on the gross and  histopathological changes in rats, also study the behaviour, blood picture and clinicl chemistry tests ( Total protein ,GOT and GPT enzymes ) in blood.  A clinical samples from (120) infected patients were taken from Urin , Stool , wound Smears and cerebrospinal fluid collected From Kendey and Yarmmuk hospitals, In Baghdad area  Thirty White male rats divided into three groups : The 1" group injected intraperitoneal with (10)' cell /ML,2nd group injected with PBS and the 3rd group (negative control ). Were used in this examination ..  The gross and histopathological Changes in (Lung, liver ,Kidney ,Muscles ,heart ,spleen ,intestine and Nervous System ) of infected rats Shows Whole damage and necrosis with congestion and haemorrhege also different kinds of inflammatory cells Infiltrated in above organs like : neutrophiles, eosinophiles and Macrophages  Blood picture showed significant P<0.05 decrease in RBCs count Hb Concentrration and PCV level, and an increase in WBC count, with decrease blood total protein and increase GPT and GOT enzymes in blood.

Carbendazim-induced haematological, biochemical and histopathological changes to the liver and kidney of male rats

2001 ◽  
Vol 20 (12) ◽  
pp. 625-630 ◽  
Author(s):  
G Selmanoğlu ◽  
N Barlas ◽  
S Songür ◽  
E A Koçskaya
Keyword(s):  
Mononuclear Cell ◽  
Biochemical Parameters ◽  
Kidney Tissue ◽  
Male Rats ◽  
Cell Infiltration ◽  
Mononuclear Cell Infiltration ◽  
Hydropic Degeneration ◽  
Wide Range ◽  
Liver And Kidney ◽  
Mean Cell Hemoglobin

Carbendazim is a systemic broad-spectrum fungicide controlling a wide range of pathogens. It is also used as a preservative in paint, textile, papermaking and leather industry, as well as a preservative of fruits. In the present study, carbendazim was administered at 0, 150, 300 and 600 mg/kg per day doses orally to male rats (Rattus rattus) for 15 weeks. At the end of the experiment, blood samples, liver and kidney tissues of each animal were taken. Serum enzyme activities, and haematological and biochemical parameters were analysed. In toxicological tests, 600 mg/kg per day doses of carbendazim caused an increase of albumin, glucose, creatinine and cholesterol levels. Also, at the same doses, white blood cell and lymphocyte counts decreased. However, mean cell hemoglobin and mean cell hemoglobin concentrations increased. Histopathological examinations revealed congestion, an enlargement of the sinusoids, an increase in the number of Kupffer cells, mononuclear cell infiltration and hydropic degeneration in the liver. At the highest doses, congestion, mononuclear cell infiltration, tubular degeneration and fibrosis were observed in the kidney tissue. These results indicate that 300 and 600 mg/kg per day carbendazim affected the liver and kidney tissue and caused some changes on haematological and biochemical parameters of rats.

scholarly journals Effect of Aqueous Extract Cyperus rotundus Tubers as Antioxidant on Liver and Kidney Functions in Albino Males Rats Exposed to Cadmium Chloride Toxic

2019 ◽  
Vol 16 (2) ◽  
pp. 0315
Author(s):  
Aldulaimi Et al.
Keyword(s):  
Uric Acid ◽  
Total Protein ◽  
Cadmium Chloride ◽  
Cyperus Rotundus ◽  
Male Rats ◽  
Oral Dosage ◽  
Control Group ◽  
Protective Effects ◽  
Liver And Kidney ◽  
Kidney Functions

          The experiment was conducted in two stages: first stage: determination of the most effective dose of Cyperus rotundus tubers aquatic extract in male rats for 5 days, which was concentrated as (200 mg/kg body weight). The second stage was designed to observe and test the protective effects of C. rotundus tubers aquatic extract in the liver and kidney functions of male rats exposed to cadmium chloride poisoning (5 mg/kg bw) for 30 days. The animals were divided into (4) groups within each group (5) animals weighted (200-220g). The results of oral dose of cadmium chloride showed a significant increase of (P˂0.05) in the activity of both enzymes Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), glucose, urea, uric acid, creatinine, malondialdehyde (MDA) and peroxynitrite (ONOO-). And a significant decrease (P˂0.05) in the values ​​of total protein, albumin, globulin and glutathione (GSH) compared with the control group. The oral dosage of the C. rotundus tubers aquatic extract resulted in a significant increase in GSH. The values ​​of each were not significantly different ALT, AST, ALP, Glucose, urea, uric acid, creatinine, total protein, albumin, globulin, MDA and peroxy nitrite Compare with control group. The results of the oral dosage with (C. rotundus tubers aquatic extract + cadmium chloride) showed a positive effect on these values ​​compared with the control group and cadmium chloride. It is concluded that the C. rotundus tubers aquatic extract has protective effects and reduces the effects that cadmium chloride can cause in rats liver and kidney functions through its antioxidant activity and removal of free radicals.

scholarly journals THE EFFECTS OF BANANA STEM (Musa paradisiaca var. sapientum) EXTRACT ON HISTOPATHOLOGIC GASTRIC OF RATS INDUCED BY INDOMETACHIN

2017 ◽  
Vol 52 (4) ◽  
pp. 246
Author(s):  
Rochmah Kurnijasanti ◽  
Arninda Alfiani Putri
Keyword(s):  
Qualitative Data ◽  
Corn Oil ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Histopathological Changes ◽  
Musa Paradisiaca ◽  
Stem Extract ◽  
Mucosa Epithelium ◽  
Banana Stem

This research aims to find out the administration effect of Ambon banana stem extract (Musa paradisiaca var. sapientum) to prevent gastric damage and observe histopathology of rat’s gastric induced by indomethacin. This research used 30 male rats age 8-12 week having 150 gram weight. They were selected randomly and divided into five groups. Negative control (K-) was given 0.5 ml CMC Na 0.5 % for 9 days and 0.5 ml corn oil was given on 10th. Positive control (K+) was given 0.5 ml CMC Na 0.5% for 9 days and then given Indomethacin emultion 5 mg/0.5 ml/150 g BW once on 10th day, and the other groups were given Ambon banana stem extract for (P1) 20 mg/150 g BW, (P2) 40 mg/150 g BW and (P3) 80 mg/150 g BW for 9 days. Afterwards, they were given Indometachin emultion 5 mg/150 g BW once on 10th day. Each gastric specimen was processed and histopathological changes were observed. Scoring of mucosa epithelium erosion and hemorrhagic, as qualitative data, was analyzed by Kruskall-Wallis test and continued by using Z test. The result shows that Pisang Ambon’s stem extract reduce significantly in gastric mucosa epithelium erosion and hemorrhagic induced by Indomethacin (p<0.05).

Melatonin Improves Blood Biochemical Parameters and DNA Integrity in the Liver and Kidney of Hyperthyroid Male Rats

2020 ◽  
Keyword(s):  
Body Weight ◽  
Dna Fragmentation ◽  
Biochemical Parameters ◽  
Mature Male ◽  
Male Rats ◽  
Dna Integrity ◽  
Tissue Samples ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Liver And Kidney

The present study was carried out to investigate and compare the effect of hyperthyroidism either alone or in combination with melatonin on some blood biochemical parameters and DNA integrity in liver and kidney of male rats. For this purpose 75 mature male rats weighing 120-140g were randomly divided into five groups (control, hyperthyroid, hyperthyroid plus 1, 5 or 10 mg/kg bwt of melatonin, respectively). Hyperthyroidism was experimentally induced by daily I/P injection of L-thyroxine (0.2 mg/kg body weight). The melatonin treated groups were injected with the same dose of L-thyroxine followed by I/P injection of melatonin (1, 5 or 10 mg/kg, respectively). The study was carried out for 21 days. The last blood and tissue samples were collected one day after the end of the last injection (on the 22nd day). The results revealed that hyperthyroidism significantly increased the levels of urea and the activities of GOT, GPT, ALP as well as the percentage of DNA fragmentation in the liver and kidney. At the same time hyperthyroidism induced a significant decrease in level of creatinine. Treatment with the 3 doses of melatonin completely ameliorated the hyperthyroidism- induced increase in GPT and ALP, while 5 and 10 mg could completely counteract the hyperthyroidism- induced increase in urea and only the 10 mg melatonin could ameliorate the hyperthyroidism-induced increase in GOT activity. It is concluded that melatonin can ameliorate the hyperthyroidism-induced disturbance in blood biochemical parameters and DNA fragmentation.

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