High-Sensitivity C-Reactive Protein in Patients with Polycystic Ovary Syndrome

2012 ◽  
Vol 19 (4) ◽  
pp. 389-396 ◽  
Author(s):  
Cristian-Ioan Iuhas ◽  
Nicolae Costin ◽  
Dan Mihu
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
High Sensitivity ◽  
Control Group ◽  
Retrospective Case ◽  
Ovary Syndrome ◽  
Visceral Fat Area ◽  
Reactive Protein ◽  
Pcos Group ◽  
The Difference

AbstractObjective: To assess the inflammation evaluated by high sensitivity C ReactiveProtein (hsCRP) in women with polycystic ovary syndrome (PCOS) compared withhealthy women without PCOS. Methods: This was a retrospective, case control,observational study. The study group included 31 patients with PCOS and 29 healthypatients matched for age and body mass index (BMI) but without PCOS (controlgroup). PCOS was diagnosed using Rotterdam criteria. Results: Patients with PCOShad higher mean hsCRP levels compared with healthy controls: 3.89±2.75 mg/l inPCOS group and 2.61±1.81 mg/l in the control group, p=0.04. The difference was nolonger significant after adjustment for BMI. In the PCOS group hsCRP waspositively correlated with BMI, waist circumference, visceral fat area, body fat massand glycated hemoglobin (HbA1c). Conclusion: hsCRP levels are increased inpatients with PCOS and are correlated with obesity, fat accumulation and not withthe presence of PCOS per se.

scholarly journals The Relevant Hormonal Levels and Diagnostic Features of Polycystic Ovary Syndrome in Adolescents

2020 ◽  
Vol 9 (6) ◽  
pp. 1831
Author(s):  
Elena Khashchenko ◽  
Elena Uvarova ◽  
Mikhail Vysokikh ◽  
Tatyana Ivanets ◽  
Lyubov Krechetova ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Polycystic Ovary Syndrome ◽  
Diagnostic Accuracy ◽  
Polycystic Ovary ◽  
Regression Function ◽  
High Sensitivity ◽  
Sex Hormone Binding Globulin ◽  
Control Group ◽  
Ovary Syndrome ◽  
Diagnostic Features

Relevance: The clinical picture of polycystic ovary syndrome (PCOS) is extremely polymorphic, especially in adolescence. At the same time, the diagnostic criteria of PCOS in adolescence are still under discussion, and the hormonal parameters, including anti-Mullerian hormone range and hyperandrogenism, are not determined. The aim of the present study was to characterize the pivotal clinical and hormonal features of PCOS in adolescents and to establish the age-specific thresholds of the most essential hormonal parameters. Design: A case-control study. Methods: The study included 130 girls with PCOS according to the complete Rotterdam criteria, aged 15 to 17 years. The control group consisted of 30 healthy girls with a regular menstrual cycle of the same age. A complete clinical and laboratory examination, hormonal assays, and ultrasound of the pelvic organs were performed. The serums anti-Mullerian hormone (AMH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), LH/FSH, prolactin, estradiol, 17α-OH progesterone (17α-OHP), androstenedione, testosterone (T), dehydroepiandrosterone sulfate (DHEAS), sex hormone-binding globulin (SHBG), leptin, and free androgen index (FAI) were analyzed. The diagnostic accuracy of AMH, FAI, LH/FSH, T, and androstenedione levels in predicting PCOS in adolescents was established using a logistic regression model and calculating area under the receiver operator characteristic (ROC) curve (AUC). Results: The serum levels of LH (9.0 (5.4–13.8) vs. 3.7 (2.5–4.7) IU/L; p < 0.0001), LH/FSH (1.6 (1.0–2.3) vs. 0.7 (0.5–1.1); p < 0.0001), 17α–OHP (4.1 (3.2–5.1) vs. 3.4 (2.7–3.8) nmol/L; p = 0.0071), cortisol (464.0 ± 147.6 vs. 284.0 ± 129.7 nmol/L; p < 0.0001), prolactin (266.0 (175.0–405.0) vs. 189.0 (142.0–269.0) mIU/L; p = 0.0141), T (1.9 (1.2–2.5) vs. 0.8 (0.7–1.1) nmol/L; p < 0.0001), androstenedione (15.8 (11.6–23.2) vs. 8.3 (6.5–10.8) ng/mL; p < 0.0001), AMH (9.5 (7.5–14.9) vs. 5.8 (3.8–6.9) ng/mL; p < 0.0001), FAI (5.5 (2.8–7.0) vs. 1.6 (1.1–2.3); p < 0.0001), SHBG (37.0 (24.7–55.5) vs. 52.9 (39.0–67.6) nmol/L; p = 0.0136), DHEAS (6.8 ± 3.2 vs. 5.1 ± 1.5 μmol/L; p = 0.0039), and leptin (38.7 ± 27.1 vs. 23.7 ± 14.0 ng/mL; p = 0.0178) were significantly altered in the PCOS patients compared to the controls. Multivariate analysis of all studied hormonal and instrumental parameters of PCOS in adolescents revealed as the most essential: AMH level > 7.20 ng/mL, FAI > 2.75, androstenedione > 11.45 ng/mL, total T > 1.15 nmol/L, LH/FSH ratio > 1.23, and the volume of each ovary > 10.70 cm3 (for each criterion sensitivity ≥ 75.0–93.0%, specificity ≥ 83.0–93.0%). The diagnostic accuracy of PCOS determination was 90.2–91.6% with the combined use of either four detected indexes, which was significantly higher than the use of each index separately. The accuracy of PCOS diagnostics reached 92% using AMH and leptin concentrations when the value of the logistic regression function [85.73 − (1.73 × AMH) − (0.12 × Leptin)] was less than 70.72. Conclusions: The results of the study estimate the threshold for AMH, FAI, androstenedione, testosterone, LH/FSH, and ovarian volume, which could be suggested for use in the PCOS diagnostics in adolescents with a high sensitivity and specificity. Moreover, the combination of either four determined indexes improved the diagnostic accuracy for the PCOS detection in adolescents.

scholarly journals Association between Luteinizing Hormone/Choriogonadotropin Receptor Ins18LQ Gene Polymorphism and Polycystic Ovary Syndrome

2020 ◽  
Vol 8 (A) ◽  
pp. 517-520
Author(s):  
Hilma Putri Lubis ◽  
Muhammad Fidel Ganis Siregar ◽  
Ichwanul Adenin ◽  
Binarwan Halim ◽  
Henry Salim Siregar ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Polycystic Ovary Syndrome ◽  
Gene Polymorphism ◽  
Polycystic Ovary ◽  
Control Group ◽  
Control Groups ◽  
Ovary Syndrome ◽  
Significant Difference ◽  
Pcos Group ◽  
And Control

BACKGROUND: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders of women in the childbearing period. However, its pathophysiology is still unclear. Certain polymorphisms of the luteinizing hormone/choriogonadotropin receptor (LHCGR) genes may lead to changes in the bioactivity of this hormone. The important functional role of LHCGR in the metabolism of androgen and ovulation, the LHCGR gene variant, may be related to the risk of PCOS. AIM: The aim of this study was to evaluate the association between LHCGR Ins18LQ gene polymorphism and PCOS. METHODS: A case–control study was performed in women with PCOS and non-PCOS from May 2019 to October 2019 in HFC IVF Center. We included 50 women with PCOS and 50 healthy controls. Polymorphism of the LHCGR (ins18LQ) gene was genotyped using polymerase chain reaction-restriction fragment length polymorphism. RESULTS: From this study, we found that there was no significant difference in the proportion of ages between the groups (p > 0.05). There were significant differences in the characteristics of body mass index, FSH level, LH level, and LH/FSH ratio between the PCOS and control groups (p < 0.05). We also found that the proportion of heterozygote variant non-ins/ins was higher in the PCOS group compared to the control group, but there was no significant difference between the polymorphisms of the non-ins and non-nonins variants between the PCOS and control groups (p = 0.269). The frequency of ins alleles was higher in the PCOS group compared to the control group. CONCLUSION: There was no significant association between LHCGR ins18LQ gene polymorphism and PCOS.

High-sensitivity C-reactive protein is a marker of obesity and not of polycystic ovary syndrome per se

2010 ◽  
Vol 94 (7) ◽  
pp. 2832-2834 ◽  
Author(s):  
Vaidyanathan Gowri ◽  
Syed Gauhar Rizvi ◽  
Shabnam Squib ◽  
Abdullah Al Futaisi
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Ovary Syndrome ◽  
Reactive Protein ◽  
Per Se

scholarly journals Υπολογισμός καρδιαγγειακού κινδύνου μέσω προσδιορισμού προγνωστικών δεικτών στους φαινότυπους του συνδρόμου των πολυκυστικών ωοθηκών

2015 ◽  
Author(s):  
Γεώργιος Δασκαλόπουλος
Keyword(s):  
Cardiovascular Disease ◽  
Child Health ◽  
Polycystic Ovary Syndrome ◽  
Human Disease ◽  
Polycystic Ovary ◽  
High Sensitivity ◽  
National Institutes Of Health ◽  
C Reactive Protein ◽  
Ovary Syndrome ◽  
Reactive Protein

Σκοπός: Το σύνδρομο των πολυκυστικών ωοθηκών [Polycystic Ovary Syndrome (PCOS)] αποτελεί τη συχνότερη ενδοκρινοπάθεια των γυναικών αναπαραγωγικής ηλικίας. Χαρακτηρίζεται από μεγάλη ετερογένεια στην κλινική εκδήλωση με κριτήρια διάγνωσης την υπερανδρογοναιμία, την ανωοθυλακιορρηξία και την πολυκυστική μορφολογία των ωοθηκών στο υπερηχογράφημα. Το σύνδρομο σχετίζεται τόσο με υπογονιμότητα όσο και με μεταβολικό και καρδιαγγειακό κίνδυνο [cardiovascular disease (CVD)]. Ο ορισμός σύμφωνα με το National Institutes of Health/National Institute of Child Health and Human Disease (NIH/NICHD), που υιοθετήθηκε το 1990, θέτει ως απαραίτητο κριτήριο για τη διάγνωση τη συνύπαρξη υπερανδρογοναιμίας και χρόνιας ανωοθυλακιορρηξίας. Με βάση αυτό τον ορισμό η επίπτωση του συνδρόμου υπολογίζεται στο 8%. Ωστόσο, σύμφωνα με τα κριτήρια του Rotterdam, που καθιερώθηκαν το 2003 στην κοινή συνάντηση ESHRE και ASRM, η διάγνωση του συνδρόμου προϋποθέτει τη συνύπαρξη δύο τουλάχιστον από τα τρία κριτήρια της υπερανδρογοναιμίας, της ανωοθυλακιορρηξίας και της πολυκυστικής μορφολογίας. Τα νέα κριτήρια του PCOS αύξησαν την επίπτωση του συνδρόμου στο 15-20%. Συνεπώς, με τα κριτήρια του Rotterdam διαγιγνώσκεται το σύνδρομο ακόμη και χωρίς την ύπαρξη υπερανδρογοναιμίας, ενώ με τα κριτήρια του 1990 δεν τίθεται διάγνωση όταν συνυπάρχουν μόνο η υπερανδρογοναιμία και η πολυκυστική μορφολογία αλλά όχι η ανωοθυλακιορρηξία (Πίνακας 1, σελ 27). Η διαφορά και η ποικιλία που γεννά η ύπαρξη όλων αυτών των διαφορετικών φαινότυπων σύμφωνα με τους δύο ορισμούς του PCOS αφορά και την εκδήλωση άλλοτε άλλου βαθμού αντίστασης στην ινσουλίνη και υπερινσουλιναιμίας, που οδηγεί σε αυξημένο κίνδυνο για δυσλιπιδαιμία, μεταβολικό σύνδρομο, σακχαρώδη διαβήτη τύπου 2, καθώς, και καρδιαγγειακό κίνδυνο. Ως εκ τούτου, η παρούσα διατριβή σχεδιάσθηκε με σκοπό τη διερεύνηση των πιθανών διαφορών στο μεταβολικό και καρδιαγγειακό κίνδυνο μεταξύ των διαφορετικών φαινότυπων του συνδρόμου των πολυκυστικών ωοθηκών με βάση τα διαγνωστικά κριτήρια του Rotterdam, ανεξάρτητα από την ηλικία και το δείκτη μάζας σώματος (ΒΜΙ).Ασθενείς-Μέθοδοι: Η μελέτη περιέλαβε 300 γυναίκες, 240 με PCOS και 60 μάρτυρες. Όλες οι γυναίκες διαιρέθηκαν με βάση το ΒΜΙ σε νορμοβαρείς και υπέρβαρες συν παχύσαρκες. Οι γυναίκες με PCOS υποδιαιρέθηκαν, επιπλέον, στους τέσσερις φαινότυπους του συνδρόμου. Διενεργήθηκε πλήρες ορμονικό και μεταβολικό προφίλ και εκτίμηση των επιπέδων των high sensitivity C reactive protein (hsCRP) και lipoprotein-associated phospholipase A2 (Lp-PLA2). Αποτελέσματα: Οι συγκεντρώσεις της hsCRP ήταν υψηλότερες αλλά δεν έφταναν τη στατιστική σημαντικότητα στις γυναίκες με PCOS σε σύγκριση με τις μάρτυρες. Στις νορμοβαρείς μάρτυρες, οι τιμές της Lp-PLA2 ήταν σημαντικά υψηλότερες σε σύγκριση με εκείνες των νορμοβαρών γυναικών, και, κυρίως, στους δύο κλασικούς φαινότυπους. Οι υπέρβαρες συν παχύσαρκες γυναίκες και στους τέσσερις φαινότυπους είχαν σημαντικά υψηλότερα επίπεδα Lp-PLA2, συγκριτικά με τις υπέρβαρες συν παχύσαρκες μάρτυρες. Ο εκτιμώμενος καρδιαγγειακός κίνδυνος και με τις τέσσερις μηχανές υπολογισμού δε διέφερε ανάμεσα στους τέσσερις φαινότυπους ούτε και ανάμεσα σε PCOS και μάρτυρες. Βρέθηκαν πολλές συσχετίσεις μεταξύ παραγόντων κινδύνου και μεταβολικού συνδρόμου, καθώς, και μη-αλκοολικής λιπώδους νόσου του ήπατος που απαιτούν θεραπευτική παρέμβαση.Συμπεράσματα: Μόνο δύο από τους τέσσερις φαινότυπους του Rotterdam που ταυτίζονται με εκείνους του κλασικού (ΝΙΗ) PCOS συνεπάγονται υψηλότερο καρδιαγγειακό κίνδυνο, ιδιαίτερα όταν συνυπάρχουν με την παχυσαρκία, και απαιτούν κατάλληλη θεραπεία.

scholarly journals Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yanna Ban ◽  
Haiying Ran ◽  
Ying Chen ◽  
Li Ma
Keyword(s):  
Pilot Study ◽  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Lipid Analysis ◽  
Polycystic Ovary ◽  
Normal Weight ◽  
Control Group ◽  
Potential Candidate ◽  
Ovary Syndrome ◽  
Pcos Group

Abstract Background The polycystic ovary syndrome (PCOS) is the most common endocrine associated with insulin resistance, even in the absence of overweight. The global lipid profile of the follicular fluid in PCOS with normal weight as yet has not been investigated. The objection of this pilot study was to explore the changes of lipids in the follicular fluid of PCOS with normal weight. Methods Follicular fluid samples were collected from patients who underwent IVF, including normal-weight women without PCOS (control group, n = 10) and normal-weight women with PCOS (PCOS group, n = 8). A lipidomic analysis was performed by high performance liquid chromatography/ mass spectrometry (HPLC-MS). Multidimensional statistical analysis was performed to disclose the global differences between the two groups. Further, differential lipid analysis between the two groups was performed by Fold Change Analysis (FC Analysis) and T-test to screen potential markers. Results All 812 species of 32 subclasses of lipids were identified by lipidomics analysis. 108 kinds of lipids were considered as the potential candidate differential metabolites with the score of variable importance in the project (VIP) more than 1 by the orthogonal partial least squares discriminant analysis. 32 lipids were significantly different between the PCOS group and the control group simultaneously with FC > 1.5 or FC < 0.67, p-value < 0.05 and VIP value > 1. These differential species of lipid belong to lipid subclasses including triglycerides (TG), phosphatidylethanolamines (PE) and phosphatidylinositols (PI). Conclusion The identified differential lipids in the follicular fluid may be considered as candidate biomarkers as well as therapeutic targets of PCOS with normal-weight.

scholarly journals Aberrant activation of the Hedgehog signaling pathway in granulosa cells from patients with polycystic ovary syndrome

2020 ◽  
Author(s):  
You Li ◽  
Guohui Xiong ◽  
Jun Tan ◽  
Shudi Wang ◽  
Ziyu Zhang ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Signaling Pathway ◽  
Granulosa Cells ◽  
Hedgehog Signaling ◽  
Polycystic Ovary ◽  
Control Group ◽  
Hedgehog Signaling Pathway ◽  
Ovary Syndrome ◽  
Ovarian Granulosa Cells ◽  
Pcos Group

Abstract The molecular mechanism that triggers polycystic ovary syndrome is mysterious. Abnormal ovarian granulosa cells are one of the causes of PCOS. Therefore, we carried out RNA-seq in ovarian granulosa cells from patients with PCOS and normal controls and found that Hedgehog signaling pathway members Ihh and ptch2 were abnormally highly expressed in the PCOS group. Granulosa cells from 22 patients with PCOS and 21 controls with normal ovulation were collected. Subsequent qPCR tests also indicated that the expression of ptch1, gli1, and gli2 of other downstream members of Hh in the PCOS group was significantly higher than that in the control group. These results indicate that abnormally activated Hh signaling pathway, especially Ihh signal, may have a profound influence on PCOS.

scholarly journals Telomere Length Differently Associated to Obesity and Hyperandrogenism in Women With Polycystic Ovary Syndrome

2021 ◽  
Vol 12 ◽  
Author(s):  
Mariela Edith Velazquez ◽  
Andrea L. Millan ◽  
Mailén Rojo ◽  
Giselle Adriana Abruzzese ◽  
Silvina Ema Cocucci ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Telomere Length ◽  
Polycystic Ovary ◽  
Major Effect ◽  
Control Group ◽  
Unrelated Donor ◽  
Ovary Syndrome ◽  
Blood Leukocytes ◽  
Real Time Quantitative Pcr ◽  
Pcos Group

BackgroundPolycystic Ovary Syndrome (PCOS) often present metabolic disorders and hyperandrogenism (HA), facts that may influence the telomere length (TL).AimsTo compare the absolute TL (aTL) between women with PCOS and control women, and their association with the presence of obesity and HA parameters.Materials and methodsThe PCOS group included 170 unrelated women outpatients and the control group, 64 unrelated donor women. Anthropometric, biochemical-clinical parameters and androgen profile were determined. The PCOS patients were divided accordingly to the presence of obesity and androgenic condition. The aTL was determined from peripheral blood leukocytes by Real Time quantitative PCR.ResultsWomen with PCOS exhibited a significantly longer aTL than controls after age adjustment (p=0.001). A stepwise multivariate linear regression in PCOS women, showed that WC (waist circumference) contributed negatively (b=-0.17) while testosterone levels contributed positively (b=7.24) to aTL. The non-Obese PCOS (noOB-PCOS) presented the longest aTL when compared to controls (p=0.001). Meanwhile, the aTL was significantly higher in the hyperandrogenic PCOS phenotype (HA-PCOS) than in the controls (p=0.001) and non hyperandrogenic PCOS phenotype (NHA-PCOS) (p=0.04). Interestingly, when considering obesity and HA parameters in PCOS, HA exerts the major effect over the aTL as non-obese HA exhibited the lengthiest aTL (23.9 ± 13.13 Kbp). Conversely, the obese NHA patients showed the shortest aTL (16.5 ± 10.59 Kbp).ConclusionsWhilst a shorter aTL could be related to the presence of obesity, a longer aTL would be associated with HA phenotype. These findings suggest a balance between the effect produced by the different metabolic and hormonal components, in PCOS women.

scholarly journals Adipokine levels and carbohydrate metabolism in patients diagnosed de novo with polycystic ovary syndrome

2021 ◽  
Vol 2021 (2) ◽  
Author(s):  
Ewelina Kolan′ska-Dams ◽  
Joanna Boinska ◽  
Maciej W. Socha
Keyword(s):  
Insulin Resistance ◽  
Polycystic Ovary Syndrome ◽  
Carbohydrate Metabolism ◽  
De Novo ◽  
Polycystic Ovary ◽  
Control Group ◽  
Model Assessment ◽  
Ovary Syndrome ◽  
Positive Correlation ◽  
Pcos Group

Introduction: Central obesity appears to play a major role in the pathogenesis of metabolic disorders in polycystic ovary syndrome. Insulin resistance and carbohydrate disorders are associated with dysfunctional secretion of various adipokines by the adipose tissue. Objectives: This study aimed to evaluate leptin, apelin, and visfatin against a background of carbohydrate metabolism parameters in patients diagnosed de novo with polycystic ovary syndrome (PCOS). Material and methods: The study group consisted of 40 patients with PCOS (mean age, 29 years) diagnosed in accordance with the American Society for Reproductive Medicine criteria from 2003. The control group consisted of 37 clinically healthy women (mean age, 26 years). All controls had regular menses and no clinical or biochemical signs of hyperandrogenism. Concentrations of leptin, apelin, visfatin, and insulin were measured by immunoenzymatic methods. Glucose concentrations were determined using spectrophotometry. Results: Significantly higher concentrations of leptin, insulin, homeostatic model assessment for insulin resistance (HOMA-IR) index, and the immunoreactive insulin (IRI)/glucose index were found in the PCOS group than in the control group. Notably, the concentration of apelin was over five times lower in the PCOS group than in the control group. In patients with PCOS, a positive correlation was found between the concentrations of insulin and leptin and concentrations of leptin and IRI/glucose. Patients of the PCOS group with body mass index (BMI) ≥  25 had significantly higher values of leptin, insulin, HOMA-IR index, and IRI/glucose index than patients of the PCOS group with normal BMI. In the PCOS group, a positive correlation was found between BMI and leptin concentration (r = 0.7176; p < 0.0001) and carbohydrate metabolism, such as insulin (r = 0.5524; p = 0.0003), glucose (r = 0.3843; p = 0.0157), HOMA-IR (r = 0.5895; p < 0.0001), and IRI/glucose (r = 0.3872; p = 0.0163). These findings were not observed in the control group. Conclusions: (1) Increased leptin concentration observed in women diagnosed de novo with PCOS as well as positive correlations between leptin and HOMA-IR, and IRI/glucose and BMI may indicate a potential role of leptin in the reduction of tissue sensitivity to insulin. (2) Significantly lower apelin concentration in the PCOS group (>5 fold) than in the control group, associated with a concomitant increase in leptin, may also contribute to carbohydrate metabolism disorders occurring in the course of PCOS.

scholarly journals TNFR1 is Upregulated and Mediates Apoptosis in Cumulus Cells of Women With Polycystic Ovarian Syndrome

2021 ◽  
Author(s):  
Yaping Jiang ◽  
Rui Jiang ◽  
Peng Zhang ◽  
qiong Yu ◽  
Hongping Ba ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Signaling Pathway ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Cumulus Cells ◽  
Control Group ◽  
Ovary Syndrome ◽  
Tnf Α ◽  
Pcos Group

Abstract Purpose To investigate the changes of human granulosa cell, TNFR1, TNFR2 and their downstream molecules in patients with polycystic ovary syndrome (PCOS) and the control group. Methods We recruited infertile women with polycystic ovary syndrome (n = 30) and compared them with infertility due to fallopian tube obstruction(n = 30, control group). The ovaries were stimulated with GnRH agonists and gonadotropins. Follicular fluid from large follicles ([14 mm]) was pooled and granulosa cells (GCs) were separated by a cellular filter. The TNF-α level of follicular fluid was measured by ELISA. TUNEL assay were used to detect the apoptosis of purified GCs. Real-time PCR and Western blotting were used to detect the expression of TNF-related signaling molecules in GCs. Results The rate of high quality embryos in the PCOS group was lower than that in the control group. There were higher percentages of apoptosis in GCs of PCOS patients than in the control group. TNF-α is upregulated in follicular fluid of PCOS patients. TNFR1 and caspase-3 mRNA level were signifificantly higher in PCOS group than in the control group. TNF-α-mediated apoptosis of PCOS granulosa cells was mainly dependent on TNFR1.The TNF-α/TNFR1 signaling pathway mediates apoptosis rather than survival in cumulus cells of PCOS patients. Conclusions TNF-α expression was upregulated in follicular fluid of PCOS patients, and TNFR1 overexpression in female granulosa cells of PCOS was associated with higher levels of apoptosis in these cells, suggesting that the TNF-α/TNFR1 signaling pathway may be a candidate for higher apoptosis in female granulosa cells of PCOS.

The facial expression of emotions recognition in patients with polycystic ovary syndrome

2016 ◽  
Vol 62 (5) ◽  
pp. 35
Author(s):  
Adam Czyzyk ◽  
Kinga Polak ◽  
Agnieszka Podfigurna ◽  
Stanislaw Kozlowski ◽  
Blazej Meczekalski
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Facial Expression ◽  
Facial Expressions ◽  
Polycystic Ovary ◽  
Control Group ◽  
Eysenck Personality Questionnaire ◽  
Ovary Syndrome ◽  
Pcos Group ◽  
Expression Of Emotions ◽  
Time Required

Background. A facial expression of emotions recognition is one of the basic psychological abilities. Sex steroids are able to strongly modulate the process of interpretation of facial expressions, as it has been shown in Turner syndrome patients.Objective. The aim of this study was the assessment of ability to interpret the facial emotions in women with polycystic ovary syndrome (PCOS).Methods. Participants completed a visual emotional task in which they were asked to recognize the emotion expressed of 80 randomly chosen facial expressions from NimStim set (Tottenham et al., 2009). With dedicated software we were able to assess the accuracy of patients facial emotion recognition (in comparison to NimStim validation set) and time required to provide the answer. Patients with psychotic personality have been excluded using Eysenck Personality Questionnaire (EPQ). All the patients underwent also hormonal tests including gonadotropins, estradiol and androgen concentrations.Patients. 80 women diagnosed with PCOS and hyperandrogenemia were included to the study. The control group consisted of 60 healthy, euovulatory women matched by age.Intervention. Each patient underwent visual emotional and EPQ tasks using specifically designed software.Main outcome measures. The accuracy rate (AR) and time required to recognize emotion (TE) of following emotions: anger, disgust, fear, happiness, sadness, surprise, calm and neutral has been measured.Results. Patients with PCOS showed significantly reduced AR for calm (0.76¬+/-0.09) and surprise (0.67+/-0.18) emotions in comparison to controls (0.81+/-0.09, 0.79+/-0.08 respectively). The TE for the anger was higher in PCOS group. Estradiol concentrations showed a statistic tendency (p=0.07) for correlation with TE for the happiness in controls. Conclusions. In this study we showed for the first time that patients affected by hyperandrogenism shows signs of disturbed recognition of facial expression of emotions. 

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