scholarly journals Morphometric Study of Connective Tissue in the Human Pituitary during Aging Process

2013 ◽  
Vol 30 (2) ◽  
pp. 79-84
Author(s):  
Miljana Pavlović ◽  
Ivan Jovanović ◽  
Snežana Pavlović ◽  
Vesna Stojanović ◽  
Vladimir Živković ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bulk Density ◽  
Blood Vessels ◽  
Aging Process ◽  
Linear Regression Analysis ◽  
Visual Fields ◽  
Test System ◽  
Morphometric Study ◽  
Human Pituitary ◽  
Network Test System

SUMMARY The aim of the research was to quantify the connective tissue and blood vessels in the aging human pituitary, using the morphometric methods. The material contained 29 pituitary tissues from cadavers of both sexes (14 females and 15 males), aged 33-95 years. The pituitary tissue was processed by standard histological procedure and stained by Mallory trichrome method. Histological samples were analyzed using a light microscope with magnification of 400x. Morphometric analysis was performed on digital pictures with the resolution of 1.3 megapixels, at 40 pituitary visual fields of each case examined. Fovea Pro 4 system was used for the analysis. Above the digital images of each field of view, there was inserted a simple network test system. Using this programme, there were determined bulk densities of connective tissue and blood vessels in the examined cases. The results were statistically analyzed using the package NCSS-PASS 2007. Correlation analysis showed that the bulk density of connective tissue statistically significantly increased during aging. The average bulk density of blood vessels in the pituitary manifested statistically significant decrease during the aging process. These results were confirmed by the linear regression analysis. The average bulk density of the connective tissue, as well as the average bulk density of blood vessels in the pituitary cases, did not differ significantly among males and females. The process of aging is followed by the proliferation of the connective tissue and fibrosis of the pituitary, which is accompanied by the reduction of its vascular network.

Research of Insulation Aging of Transformer Insulation Paper by Multi-Parameter Regression Analysis

2011 ◽  
Vol 233-235 ◽  
pp. 1345-1348
Author(s):  
Dao Wu Yang ◽  
Hui Hui Han ◽  
Su Wang ◽  
Hai Xia Tong ◽  
Zhuo Ren
Keyword(s):  
Regression Analysis ◽  
Water Content ◽  
Aging Process ◽  
Linear Regression Analysis ◽  
Functional Relation ◽  
Multiple Linear Regression Analysis ◽  
Degree Of Polymerization ◽  
Insulation Aging ◽  
Service Period ◽  
Transformer Insulation

In this paper two types of oil-paper samples are thermally aged in two temperatures of 120. The samples are constituted by 25# oil, 45# oil and paper. Some aging indicators are measured during the aging process. Three parameters are measured during the aging process. The insulating ability of paper can be evaluated by water content and CO2/CO value. The model of functional relation for degree of polymerization (DP) with CO2/CO value, water content and service period are established by multiple linear regression analysis.

Visual Field Damage and Progression in Glaucomatous Myopic Eyes

2007 ◽  
Vol 17 (4) ◽  
pp. 534-537 ◽  
Author(s):  
A. Perdicchi ◽  
M. Iester ◽  
G. Scuderi ◽  
S. Amodeo ◽  
E.M. Medori ◽  
...  
Keyword(s):  
Visual Field ◽  
Open Angle Glaucoma ◽  
Linear Regression Analysis ◽  
Visual Fields ◽  
Light Sensitivity ◽  
Progressive Increase ◽  
Central Visual Field ◽  
Trend Function ◽  
Visual Field Damage ◽  
Loss Variance

Purpose To make a visual field retrospective analysis on a group of patients with primary open angle glaucoma (POAG) and to evaluate whether different refractive errors could have different progression of the 30° central sensitivity. Methods A total of 110 patients with POAG (52 men and 58 women) were included in the study. All the patients were divided into four subgroups based on the refractive error. The visual field of all the included patients was assessed by an Octopus 30° central visual field every 6 months, for a total of 837 visual fields examined. The resulting data were analyzed by PERIDATA for Windows 1.7 TREND function. Mean defect (MD) and loss variance (LV) were considered for the analysis. Results At the first examination, 82% of eyes showed a global decrease of differential light sensitivity (MD >2 dB) and in 67% the distribution of the defect was nonhomogeneous (LV >6 dB). The analysis of variance for subgroups showed a more significant decrease of MD in highly myopic patients. A linear regression analysis highlighted a statistically significant change in time of MD in 36% and of LV in 34% of the eyes studied. Highly myopic patients had the highest (p<0.01) percentage of change of MD and LV (46% and 42%, respectively). Among the four subgroups, there was no difference in progression of MD decrease in time. Conclusions These results showed that after 5 years of glaucoma, the visual field was altered in most of the eyes examined (82%) and that in 67% of cases, its defect was nonhomogeneous and worsened with the increase of myopia. The regression linear analysis of visual field changes in time showed a progressive increase of MD and LV in approximately one third of all the eyes examined.

scholarly journals Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Vesna R. Stojanović ◽  
Ivan D. Jovanović ◽  
Sladjana Z. Ugrenović ◽  
Ljiljana P. Vasović ◽  
Vladimir S. Živković ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Connective Tissue ◽  
Morphometric Analysis ◽  
Aging Process ◽  
Systemic Disease ◽  
Digital Camera ◽  
Morphometric Parameters ◽  
Tissue Content ◽  
Tissue Area ◽  
Tissue Specimens

Number of sclerotic glomeruli increases during the aging process. Consequently, majority of remained nonsclerosed glomeruli become hypertrophic and some of them sclerotic, too. The aim of this study was to quantify the size and connective tissue content of nonsclerosed glomeruli and to evaluate the percentage of hypertrophic ones in examined human cases during the aging. Material was right kidney's tissue of 30 cadavers obtained during routine autopsies. Cadavers were without previously diagnosed kidney disease, diabetes, hypertension, or any other systemic disease. Tissue specimens were routinely prepared for histological and morphometric analysis. Images of the histological slices were analyzed and captured under 400x magnification with digital camera. Further they were morphometrically and statistically analyzed with ImageJ and NCSS-PASS software. Multiple and linear regression of obtained morphometric parameters showed significant increase of glomerular connective tissue area and percentage. Cluster analysis showed the presence of two types of glomeruli. Second type was characterized with significantly larger size, connective tissue content, and significantly lower cellularity, in relation to the first type. Such glomeruli might be considered as hypertrophic. First type of glomeruli was predominant in younger cases, while second type of glomeruli was predominant in cases older than 55 years.

scholarly journals Enamel Matrix Derivative in Diffusion Chamber Implanted Subcutaneously in Rat Induces Formation of Fibrous Connective Tissue Containing Abundant Blood Vessels

In Vivo ◽  
2021 ◽  
Vol 35 (1) ◽  
pp. 313-317
Author(s):  
SATOSHI YOKOSE ◽  
YUKA KATO ◽  
KATSUTOSHI MATSUMOTO ◽  
PERRY R. KLOKKEVOLD ◽  
HENRY H. TAKEI ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Diffusion Chamber ◽  
Enamel Matrix Derivative ◽  
Fibrous Connective Tissue ◽  
Enamel Matrix ◽  
Matrix Derivative

scholarly journals Morphofunctional and immunohistochemical characteristics of the large omentum in ovarian cancer

2020 ◽  
Vol 9 (3) ◽  
pp. 64-71
Author(s):  
N. N. Shevlyuk ◽  
L. V. Khalikova ◽  
A. A. Khalikov
Keyword(s):  
Ovarian Cancer ◽  
Connective Tissue ◽  
Protein Expression ◽  
Blood Vessels ◽  
Lymphoid Tissue ◽  
P53 Protein ◽  
Individual Variability ◽  
The Body ◽  
Great Omentum ◽  
Low Grade

The aim of the study was to establish morphofunctional and immunohistochemical characteristics of large omentum in women with ovarian cancer.Material and methods. The large omenta of 48 women with ovarian cancer (low-grade differentiated seropapillary adenocarcinoma of high-grade malignancy) of II stage (n=20) and III stage (n=28) were studied. Histological sections were stained with overview histological and immunohistochemical methods (to reveal ki67, P53, CD34, CD7, CD4, CD8, CD61 proteins expression). Results. In patients, the size of the large omentum was characterized by high individual variability; in the presence of metastasis, the size of the omentum was reduced. Intensive development of blood vessels in the organ was noted, but in the presence of metastases stasis of blood corpuscles, leucocytic infiltration, and moderate edema of connective tissue were observed in the organ’s vessels. Areas of lymphoid tissue, both small lymphatic follicles and diffusely located lymphoid tissue, were revealed in the omentum. In most follicles, reactive centers were not marked, and the number of follicles was reduced in the presence of metastases in the omentum. The analysis of CD34+ cells distribution showed that they were identified both in the tumor and in the areas of the omentum adjacent to the tumor, which indicates a pronounced angiogenesis. An irregular distribution of CD7+ and CD8+ and CD4+ cells was revealed in the tumor tissues, as well as in the surroundings. Simultaneously with the expression of P53 protein, ki67 protein expression is revealed in the significant number of tumor cells (including endothelial cells of tumor blood vessels). The proportion of ki67+ cells in the tumor cell population was 60.1±3.3%. The presence of a large number of ki67+cells in the presence of P53 protein expression in them indicates the aggressiveness of the tumor, as well as a disturbance of apoptosis regulatory mechanisms in the cells. Ki67 expression was low in the omentum areas unaffected by metastases, and it was revealed in the certain areas of connective tissue in fibroblastic programmed differentiation cells. Conclusion. The results obtained indicate significant plasticity and reactivity of great omentum in the presence of tumor process in the body and confirm the important role of great omentum in protective reactions.

scholarly journals FACTORS INFLUENCING THE INTERMITTENT PASSAGE OF LOCKE'S SOLUTION INTO LIVING SKIN

1941 ◽  
Vol 73 (1) ◽  
pp. 85-108 ◽  
Author(s):  
Philip D. McMaster
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Atmospheric Pressure ◽  
Interstitial Fluid ◽  
Lymphatic Vessels ◽  
Intermittent Flow ◽  
Venous Obstruction ◽  
Factors Influencing ◽  
Interstitial Connective Tissue ◽  
Living Mouse

Minute amounts of Locke's or Tyrode's solution have been brought into contact with the interstitial connective tissue of the skin of the living mouse, at atmospheric pressure, in such a manner that the blood or lymphatic vessels are not entered directly. Under such circumstances these absorbable fluids enter the tissue spontaneously. Entrance is strikingly intermittent, not continuous, and so too when very slight pressures are brought to bear on the fluids (1). Hyperemia of the tissues, with accompanying dilatation of the blood vessels, increases the entrance of fluids at atmospheric pressure but it is still intermittent. By contrast, venous obstruction leads to intermittent backflow into the apparatus, but reflex hyperemia, following release of the obstruction, is attended by an increase of flow into the tissues in spite of the great reactive dilatation of vessels. The inflow is also intermittent. If the skin is deprived of circulation, fluid does not enter it at all at atmospheric pressure, though it moves in regularly and continuously if slight pressure is put upon it. Edema-forming fluids, described in the text, also enter in a continuous manner when forced into the skin of either living or dead animals. So too do serum and sperm oil. The findings indicate that the passage of interstitial fluid into the blood vessels may be intermittent under normal circumstances and its escape from them as well. The observed occurrence of intermittent flow in the blood vessels of several tissues (9, 15–25) will go far to account for the intermittent entrance of fluid into the skin.

On the Pituitary of the Perch (Peroa Fluviatilis)

1942 ◽  
Vol s2-83 (331) ◽  
pp. 299-316
Author(s):  
T. KERR
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Perca Fluviatilis ◽  
Cell Types ◽  
Anterior Lobe ◽  
Intermediate Lobe ◽  
General Description ◽  
Perch Perca Fluviatilis ◽  
Higher Types ◽  
Different Cell Types

1. A general description is given of the pituitary of the perch (Perca fluviatilis L.), and histological details of its various parts. The subdivisions of the glandular component are confluent with each other but distinguished by their different cell types. The nervous lobe makes contact with all three of the subdivisions, but is separated from them by a layer of connective tissue, incomplete in particular areas. 2. The anterior glandular region (anterior lobe) has an anterior chromophil and a posterior chromophobe zone. The middle glandular region (transitional lobe) possesses brightly staining acidophils and basophils as well as chromophobes. The acidophils form a dorsal sheet, deeply indented by processes of the nervous lobe, the basophils lie ventrally and posteriorly, and chromophobes are common towards the extremities of the indentations. The posterior glandular region (intermediate lobe) is elaborately penetrated by nervous lobe processes; the cells are small and consist of amphiphils, dull basophils, and occasional dull acidophils. The possible homologies of these regions to the lobes of higher types are discussed. The nervous lobe is of loose glial tissue with many nuclei and blood vessels and some reticular and collagenous fibres. 3. Strongly acidophil spheres of various sizes and in various numbers occur in the middle glandular region. They originate in ‘sphere cells’ resembling eosinophil leucocytes and after enlarging become free in the tissues of the region. Later they appear to pass into the posterior processes of the nervous lobe to be the larger bodies of the Herring material. Finally these larger elements appear to break down to form a fine granulation, whose further fate could not be followed.

Light and Scanning Electron Microscopy of Human Spinal Ligaments

1985 ◽  
Vol 55 ◽  
Author(s):  
L.-H. Yahia ◽  
G. Drouin ◽  
C.-H. Rivard
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Connective Tissue ◽  
Idiopathic Scoliosis ◽  
Blood Vessels ◽  
Structural Changes ◽  
Loose Connective Tissue ◽  
Morphological Studies ◽  
Spinal Ligaments ◽  
Scanning Electron

ABSTRACTSpinal ligaments were obtained from normal and scoliotic individuals. Detailed morphological studies were carried out on the yellow, interspinous and supraspinous ligaments by light and scanning electron microscopy. Normal yellow ligaments are mostly constituted of dense elastin fibers with only a few collagen fibers and sparse blood vessels, while in normal interspinous and supraspinous ligaments, the presence of collagen is highly dominant. In the latter structures, the collagen fascicles are characterized by a regular waviness morphology. The fibrils constituting the fascicles appear either parallel or helical with respect to the fascicle axis. Structural changes are observed in the spinal ligaments of patients with congenital as well as idiopathic scoliosis. For yellow ligaments, only slight differences are found between normal and scoliotic specimens. However, alterations in collagen waviness and architecture are observed mainly in the supraspinous ligaments and to a lesser extent in the interspinous ligaments. In addition, increases in the cellularity, loose connective tissue and vessels are found in both forgoing ligaments. These results indicate that the more pronounced scoliosis-related changes occur in ligaments having the farthest distance from the axis of flexion-rotation.

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