scholarly journals Hormone Priming Improves Germination Characteristics and Enzyme Activity of Sorghum Seeds (Sorghum Bicolor L.) Under Accelerated Aging

2013 ◽  
Vol 46 (3) ◽  
pp. 49-56 ◽  
Author(s):  
M.S. Azadi ◽  
S.A. Tabatabaei ◽  
E. Younesi ◽  
M.R. Rostami ◽  
M. Mombeni
Keyword(s):  
Enzyme Activity ◽  
Accelerated Aging ◽  
Aging Treatment ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Germination Index ◽  
Percentage Germination ◽  
Germination Characteristics ◽  
Aged Seeds

Abstract Maximum germination percentage achieves immediately after harvesting and gradually decreases with storage time. Aging is one of the key factors in plant yield loss especially in vegetables. Seed aging is the main problem of seed storage. Application of accelerated aging treatment is used to assess seed vigor and quality. Seed priming enhances seed germination performance after aging. An experiment was conduct in order to investigate the activity of catalase and ascorbate peroxidase during accelerated aging and repair during priming treatment of sorghum seeds. In order to improve germination characteristics in aged seeds with seed priming. Our result showed that seed priming treatments significantly (p≤ 0.01) affected, germination percentage, germination index and means time to germination after aging (0, 3 and 6 days). Increasing aging duration resulted higher reduction in germination characteristics. Priming with gibberelic acid (GA), salicylic acid (SA) and ascorbic acid (ASC) increased germination characteristics of seed aged. The highest germination percentage, germination index, normal seedling percentage and enzyme activity were achieved in control conditions (0 day of aging). Antioxidant activity of aged seeds increased after seed priming.

scholarly journals The Changes of Germination Characteristics and Enzyme Activity of Barley Seeds under Accelerated Aging

2015 ◽  
Vol 48 (2) ◽  
pp. 61-67 ◽  
Author(s):  
S A. Tabatabaei
Keyword(s):  
Enzyme Activity ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Germination Percentage ◽  
Germination Index ◽  
Accelerated Ageing ◽  
Percentage Germination ◽  
Seed Aging ◽  
Germination Characteristics ◽  
Normal Seedling

Abstract Seed aging is the main problem of seed storage. Changes of enzyme activity and reduction of seedling growth are consequence of seed deterioration. An experiment was conducted to evaluate the effects of accelerated aging on germination indexes and enzyme activity of barley seeds. Seeds were incubated in closed plastic boxes for the accelerated aging treatments at 41°C. Three accelerate aging regimes were performed by placing seeds at 41°C and relative humidity (RH) of 90-100 % for 0, 4 and 8 day periods. Our results showed that increasing aging duration resulted higher reduction in germination percentage, germination index, mean time to germination, normal seedling percentage, catalase and ascorbate peroxidase. The highest germination percentage, germination index, normal seedling percentage and enzyme activity were achieved in control conditions (0 day of aging). Under aging conditions, germination percentage, means time to germination, germination index, normal seedling percentage and enzyme activity decrease significantly. Also, our results indicated that seed aging is related to decrease of enzymes and may contribute to low germination efficiency. The general decreases in enzyme activity in the seed lowers the respiratory capacity, which in turn lowers both the energy (ATP) and assimilates supply of the germinating seed, also decrease in antioxidant enzymes is linked to an increased accelerated ageing and decreased germination characteristics. Subsequently, proposed a positive relationship between antioxidant enzyme capacity and the vigour of the seed

scholarly journals Improvement of Germination Characteristics and Enhancement of Antioxidant Enzymes Activity of Safflower (Carthamus tinctorius L.) Aged Seeds by Used of Gibberellin

2015 ◽  
Vol 48 (3) ◽  
pp. 33-41
Author(s):  
M. Khajeh ◽  
S.A. Tabatabaei ◽  
O. Ansari ◽  
F. Sharif Zadeh
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzymes ◽  
Ascorbate Peroxidase ◽  
Seed Priming ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Characteristics ◽  
Normal Seedling

AbstractSeed priming is one of the methods that can be taken to counteract the adverse effects of abiotic stress, also Seed priming treatments have been used to reduce the damage of aging and invigorate their performance in many crops. Objective of this study was to evaluation the effect of gibberellin on germination characteristics and antioxidant enzymes of safflower seeds after aging. Experimental design was a factorial one with complete randomized design with three replications. The first factor was priming by gibberellin (0 and 50 ppm) and non-primed seeds (control) and the secondary factor was combinations of four levels of aging (0, 1, 3 and 5 days of aging, at 43°C). The results showed that the priming and aging effects on germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were significant, but on mean time to germination not significant. Results showed that, the highest germination characteristics such as; germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were attained from priming by gibberellins, under non aged conditions. Also, our results indicated that seed aging is related to decrease of enzymes activity and may contribute to low seed germination efficiency, also priming increases enzyme activity and increases enzyme activity with priming treatment may contribute to improve germination characteristics. The general decreases in enzyme activity in the seed lowers the respiratory capacity, which in turn lowers both the energy (ATP) and assimilates supply of the germinating seed.

scholarly journals Muskmelon seed priming in relation to seed vigor

2004 ◽  
Vol 61 (1) ◽  
pp. 114-117 ◽  
Author(s):  
Warley Marcos Nascimento ◽  
Fernando Antônio Souza de Aragão
Keyword(s):  
Low Temperature ◽  
Interaction Effect ◽  
Cucumis Melo ◽  
Seed Quality ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Cucumis Melo L ◽  
Aged Seeds

A number of important factors may affect seed priming response, including seed quality. Effects of seed vigor on seed priming response were investigated using seed lots of two muskmelon (Cucumis melo L.) cultivars. Seeds of muskmelon, cvs. Mission and Top Net SR were artificially aged at 43°C for 0, 20 and 40 hours. Seeds were primed for six days in darkness at 25°C in KNO3 (0.35 mol L-1) aerated solution. Aged seeds germinated poorly at 17°C. Priming increased germination rate at 17 and 25°C and germination percentage at 17°C. An interaction effect on germination performance between vigor and priming was observed, especially at low temperature. Priming increased germination performance in seeds of low vigor, and the response was cultivar dependent.

scholarly journals Influence of seed priming on germination characteristics of sorghum (Sorghum bicolor L. Moench)

2021 ◽  
Vol 911 (1) ◽  
pp. 012086
Author(s):  
Ramlah Arief ◽  
Fauziah Koes ◽  
Oom Komalasari ◽  
Fatmawati
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
Primary Root ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Seed Vigor ◽  
Germination Characteristics ◽  
Positive Effect

Abstract Seed priming or invigoration is presowing treatments of improving germination aimed to reduce the time from sowing to emergence and improving emergence uniformity. Research on seed priming was conducted Indonesian Cereal Research Institute (ICERI) seed laboratory from January until March 2021 to evaluate the effect of seed priming on sorghum seed vigor. Seed quality parameters includes germination percentages, germination rate, shoot and primary root length, seedling dryweight, Sorghum seed variety of Suri 4 were used as seed material. Results showed that among the priming treatments that give positive effect on germination percentage, germination rate, seedling dry weight were priming treatment with KNO3 1.5%.

scholarly journals Ultrasonic Assisted Seed Priming to Alleviate Aging Damages to Milk Thistle (Silybum marianum) Seeds

2018 ◽  
Vol 10 (2) ◽  
pp. 275-281
Author(s):  
Seyed A. MOOSAVI ◽  
Seyed A. SIADAT ◽  
Adel POSHTDAR ◽  
Fatemeh DIREKVAND
Keyword(s):  
Unsaturated Fatty Acids ◽  
Accelerated Aging ◽  
Aging Treatment ◽  
Seed Priming ◽  
Milk Thistle ◽  
Silybum Marianum ◽  
Ultrasonic Assisted ◽  
Aging Conditions ◽  
Pharmaceutical Properties ◽  
Aged Seeds

Milk thistle is a medicinal plant with high pharmaceutical properties to help relief of liver diseases. In this study, the effects of ultrasonic assisted seeds priming (20, 40, 80 and 160 s) with a frequency of 24 kHz and Power of 400 W was investigated on seed enhancement of aged seeds of Milk thistle. Results of the study, showed that as the aging damages increased, the longer sonication results in the better germination. Root growth was significantly improved using ultrasonic energy. Seeds aged for 24 hours, were exposed to ultrasonic produced for 20 seconds exhibited root length of 10.39 cm which was 5.48 cm with no ultrasonic treatment with the same aging duration. The maximum malondialdehyde activity was observed at the 96-hour aging treatment (87.83 nmol/grFW) while the lowest activity was observed at no aged and 24-hour aged seeds (7.28 nmol/grFW). It is suggested that there is a negative correlation between seedling vigor and MAD activity. It was revealed that fatty acid composition of Silybum marianum seed oil is highly influenced by the aging treatment. The variations of unsaturated fatty acids significantly increased in aged seeds. Our results showed that under accelerated aging conditions, ultrasonic assisted seed priming could not provide satisfactory enhancement to seed germination while no aged seeds germination was significantly improved using ultrasonic assisted seed priming.

scholarly journals The Effect of Seed Priming and Accelerated Aging on Germination and Physiochemical Changes in Milk Thistle (Silybum marianum)

2013 ◽  
Vol 5 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Ghasem PARMOON ◽  
Ali EBADI ◽  
Sodabeh JAHANBAKHSH ◽  
Mahdi DAVARI
Keyword(s):  
Polyphenol Oxidase ◽  
Seedling Growth ◽  
Germination Rate ◽  
Accelerated Aging ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Milk Thistle ◽  
Antioxidants Activity ◽  
Germination Speed

Effects of seed priming and aging on some physiological characteristics of Milk thistle was studied in a factoral experiment based on Complete Randomized Design (CRD). Tratments were included hydro priming (using distilled water), halo priming (0, 1.5, 3, 4.5 and 6% KNO3) and accelerated aging (0, 2, 4 and 6 days under 45°C and 95% humidity) in three replications. Determined parameters were germination charactristics including germination percentage, daily germination speed, mean time of germination, seed vigor index, hypocutile length and hypocutile dry weight. Activity of catalase, peroxidase and polyphenol oxidase were determined at 12 hours after imbibition and seedling stage. According to results of this experiment, germination percentage, seed vigor and seedling growth of seeds were increased under all priming treatments. Improving the catalase and peroxidase activity led to decrease the aging damages. Germination characteristics were improved under both priming treatments at the beginning of germination as well as seedling growth. Polyphenol oxidase activity was increased in the pre-treated seeds but decreased in seedling growth stage. Aging treatments led to reduce the germination percentage, daily germination speed, seed vigor and seedling growth while the germination time was increased. Accelerated aging caused to reduce the germination rate and seedling growth of milk thistle that is probably due to increasing the lipid peroxidation, free radical increment and decreasing the antioxidants activity. The greatest and lowest antioxidants activity, the germination percentage, germination speed and seed vigor were respectively observed under priming using 3% KNO3 concentration and control seeds.

HEAT SHOCK TREATMENT CAN IMPROVE SOME SEED GERMINATION INDEXES AND ENZYME ACTIVITY IN PRIMED SEEDS WITH GIBBERELLIN OF MOUNTAIN RYE (SECALE MONTANUM) UNDER ACCELERATED AGING CONDITIONS

2013 ◽  
Vol 46 (4) ◽  
pp. 21-30
Author(s):  
O. Ansari ◽  
F. Sharif-Zadeh ◽  
A. Moradi ◽  
M.S. Azadi ◽  
E. Younesi
Keyword(s):  
Enzyme Activity ◽  
Heat Shock ◽  
Seed Germination ◽  
Accelerated Aging ◽  
Shock Treatment ◽  
Seed Vigor ◽  
Heat Shock Treatment ◽  
Significant Difference ◽  
Secale Montanum ◽  
Aged Seeds

ABSTRACT Seed priming with gibberellin (GA) enhances seed germination performance; but the quality of primed seeds in aging condition often reduces more than non-primed seeds. An experiment was conducted to evaluate the effect(s) of heat shock treatments on germination characteristics and enzyme activity of primed mountain rye (Secale montanum) seeds with gibberellin under accelerated aging. Heat shock treatments, can substantially decrease the speed of quality reduction of mountain rye (Secale montanum) primed seeds. In primed seeds with gibberellin, which has non-aged, the highest germination percentage (GP) and normal seedling percentage (NSP) was attained from heat shock treatment at 35°C for 3 h, also after 3 days aging, it was attained from heat shock treatment at 35°C for 3 h. After 3 days of aging the highest germination index (GI) was attained from unprimed seeds, but no significant difference with heat shock treatment at 35°C for 3 h. The minimum means time germination (MTG) was in heat shock treatment at 30°C for 3 h in non-aged seeds. After 3 days of aging, heat shock treatment reduce MTG as compared to the primed seeds. Heat shock treatment at 35°C for 3 h increased seed vigor index (SVI) as compared to the unprimed and primed seed in non-aged seeds and after 3 days aging. Seedling length (SL) increases with heat shock treatment at 30°C for 4 h in non-aged seeds as compared to the primed and unprimed seeds, but after 3 days of aging heat shock treatment except at 35°C for 3 h and 40°C for 4 h reduced SL as compared to the primed and unprimed seeds. Also, heat shock treatments increase some antioxidant enzymes [Catalase (CAT), Ascorbat peroxidase (APX)].

scholarly journals Germination Characteristics of Three Warm-season Turfgrasses Subjected to Matriconditioning and Aging

2007 ◽  
Vol 17 (4) ◽  
pp. 480-485 ◽  
Author(s):  
Gokhan Hacisalihoglu
Keyword(s):  
Cynodon Dactylon ◽  
Warm Season ◽  
Accelerated Aging ◽  
Aging Treatment ◽  
Germination Percentage ◽  
Paspalum Notatum ◽  
Germination Time ◽  
Germination Characteristics ◽  
Mean Germination Time ◽  
Eremochloa Ophiuroides

Many warm-season turfgrass seeds have relatively poor germination percentages. Matriconditioning is a seed enhancement technique with a solid carrier and may be a practical solution to improve the germination characteristics of warm-season turfgrass. The objective of this study was to determine the effectiveness of matriconditioning on three nonaged and aged turfgrass cultivars: ‘Pensacola’ bahiagrass (Paspalum notatum), ‘Princess’ bermudagrass (Cynodon dactylon), and ‘Common’ centipedegrass (Eremochloa ophiuroides). Seeds were matriconditioned with a synthetic calcium silicate (MicroCel E) as a carrier and water at 30 °C for 5 days. Seed, carrier, and water ratio was 1 g, 0.5 g, and 1.5 mL, respectively. Matriconditioning increased final germination to 55% (bahiagrass), 90% (bermudagrass), and 70% (centipedegrass) compared with 92% in nontreated control seeds. Furthermore, matriconditioning decreased mean germination time 20% to 65% in all seeds compared with the nontreated control. Accelerated aging was induced by storing seeds for 0, 7, and 14 days at 42 °C and 95% relative humidity. Germination percentage decreased and mean germination time increased with the aging, especially after 14 days of aging treatment. These results suggest that matriconditioning is an effective technique to improve turfgrass seed performance.

scholarly journals Seed Priming Improves Seed Germination and Seedling Growth of Isatis indigotica Fort. under Salt Stress

HortScience ◽  
2020 ◽  
Vol 55 (5) ◽  
pp. 647-650
Author(s):  
Xu-Wen Jiang ◽  
Cheng-Ran Zhang ◽  
Wei-Hua Wang ◽  
Guang-Hai Xu ◽  
Hai-Yan Zhang
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Antioxidant Enzyme Activities ◽  
Isatis Indigotica ◽  
Germination Characteristics

The effects of CaCl2, GA3, and H2O2 priming on Isatis indigotica Fort. seed germination characteristics, seedling growth parameters, and antioxidant enzyme activities under salt stress were investigated. NaCl had an adverse effect on the germination and seedling performance of I. indigotica. However, these three priming agents alleviated salt stress by increasing the germination percentage, improving seed vigor, accelerating germination velocity, and establishing strong seedlings. The optimal concentrations were 15 g/L for CaCl2, 0.2 g/L for GA3, and 40 mm for H2O2. Seed priming treatments enhanced the activities of antioxidant enzymes in seedlings, such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), under a salt environment, which reduced the oxidative injury caused by salt. Seed priming is a promising technique that can enhance the ability of I. indigotica seed germination when salt is present.

scholarly journals Biochemical investigations on vigour enhancement in aged seeds upon seed priming in onion

2016 ◽  
Vol 8 (2) ◽  
pp. 855-859 ◽  
Author(s):  
Umesha Umesha ◽  
S.N. Vasudevan ◽  
K. Bhanuprakash ◽  
B. Manjunatha ◽  
G. Sarika ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Seed Quality ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigour ◽  
Protein Levels ◽  
Seed Deterioration ◽  
Metabolic Defects ◽  
Induced Proteins ◽  
Aged Seeds

Loss in seed quality that occurs during all process, from maturation in the field to storage, leads to seed deterioration. Storage of onion seeds under ambient, hot and humid conditions is very problematic since these conditions deteriorate seed quality faster. Seed deterioration is associated with many metabolic defects that occur due to changes in enzymes and protein levels. This deterioration can be rectified to the extent possible by the technique of seed priming. The results clearly indicated that priming will restore the lost seed vigour in aged seeds due to reactivation of enzyme activity in old seeds. The germination percentage improved from 60% to 79.5% and 72.5% in GA3 and hydroprimed, respectively. Priming will also restore the lost seed vigour in aged seeds due to reactivation of proteins in old seeds and expression of these proteins in priming treatments are related to priming induced proteins in contrast to their absence in the aged seeds which are necessary for germination and longevity of seeds.

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