scholarly journals Evaluation of wound healing potential of a sea cucumber (Actinopyga mauritiana) extract in mice (Mus musculus)

2021 ◽  
Vol 21 (3) ◽  
pp. 168-174
Author(s):  
ARFANI ARFANI ◽  
AHMAD RAIF ◽  
CHRISMIS NOVALINDA GINTING ◽  
REFI IKHTIARI
Keyword(s):  
Wound Healing ◽  
Sea Cucumber ◽  
Biological Activities ◽  
Ethanol Extract ◽  
Density Level ◽  
Marine Source ◽  
Qualitative Test ◽  
Wound Length ◽  
Wounded Area ◽  
Wound Healing Activity

Sea cucumbers are a marine source with biological activities that have been used in food as well as medicine in many Asian countries. Actinopyga mauritiana is one of the sea cucumber species with limited research about its bioactive activities. In this study, the wound healing activity of A. mauritiana extract in the form of cream, gel, and serum formulations in mice was investigated. The histopathology test was performed to evaluate the fibroblast and collagen dense levels in wound healing. Phytochemical screening has been carried out by the qualitative test of alkaloids, flavonoids, glycosides, steroids, and saponins compounds. The A. mauritiana ethanol extract (SCAE) was added into cream, gel and serum formulations with various concentrations (3, 6, and 9%) and applied to the wounded area of mice. The wound healing activities and histopathology results indicated that serum 9%-SCAE performed the highest decrease of wound length 0.55 ± 0.32 compared to other groups with a density level of fibroblast and collagen are 10.00 ± 1.00 and 10.67 ± 0.00, respectively. To the best of our knowledge, this is the first report on the wound healing activity of A.Mauritiana, which demonstrated promising therapeutic agents for wound healing and cosmetics applications in the future.

scholarly journals Wound Healing Activity of Ethanolic Extract Gel of Tawas Ut Tuber (Ampelocissus rubiginosa L.) in Incisional Model Wistar Rats

2018 ◽  
Vol 23 (1) ◽  
pp. 30 ◽  
Author(s):  
Khoerul Anwar ◽  
Dewita Fitri Widodo ◽  
Nurlely Nurlely ◽  
Liling Triyasmono ◽  
Sudarsono Sudarsono ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Average Percentage ◽  
Wound Length ◽  
Wound Healing Activity

Tawas ut tuber (Ampelocissus rubiginosa Lauterb.) is empirically used in wound healing by Dayak community. The present study was undertaken to assess wound healing activity of gel from ethanol extract of A. rubiginosa tuber using parameters of the closure of wound length, tensile strength, and histopathological observations. This study used twenty-five male Wistar rats divided into five groups: positive control (Bioplacenton®), negative control (placebo gel), and three of testing groups with gel extract (concentrations of 1.5%, 2.0%, and 2.5%). Wound healing activity was evaluated to 4 cm length and ± 2 mm depth incision wound model on the back skin of rats. The measurement of wound length was observed on the day of 4th, 8th, 12th, 16th, and 20th used quantitative analyze. On the day of 20th, animal was anaesthetized and the skin has been taken for tensile strength evaluation and histopathological observations. This study showed that group treated with gel extract at the concentration of 2.5% experienced higher wound healing activity with average percentage of wound closure of 99.00% ± 0.16, tensile strength 3.8541 gram/mm2, and proved with the re-epithelization, neocapillarization, and increase collagen density appeared in histopathological observations. Based on this study, gel from ethanol extract of A. rubiginosa tuber possesses wound healing activity.

scholarly journals ROSELLE CALYX ETHANOL EXTRACT STIMULATES ORAL MUCOSAL WOUND HEALING

2018 ◽  
Vol 9 ◽  
pp. 36
Author(s):  
Sarwono At ◽  
Suniarti Df
Keyword(s):  
Wound Healing ◽  
Ethanol Extract ◽  
Lymphocytic Infiltration ◽  
Punch Biopsy ◽  
Sprague Dawley ◽  
Standard Temperature ◽  
Sprague Dawley Rats ◽  
Similar Formation ◽  
Wounded Area ◽  
Mucosal Wound

Objective: This study aim to analyze the effect of the roselle calyx ethanol extract on oral mucosal wound healing.Methods: Male Sprague Dawley rats (3 month, 150 g) were housed under standard temperature conditions (20–22°C). Punch biopsy wounds were inflicted on the inferior labial mucosal membrane of rats. All treatments (normal saline; 0.1% triamcinolone acetonide; and 3.75%, 7.5%, and 15% roselle calyx ethanol extract) were applied 2 times daily. Wounded areas were observed after 8 days of treatment. The specimen of the wounded area was prepared and microscopic slides were analyzed under a light microscope.Results: The histological features of the groups treated with TA and 7.5% roselle calyx ethanol extract showed a similar formation of the epidermis layer and no lymphocytic infiltration of the lamina propria.Conclusion: The application of 7.5% roselle calyx ethanol extract could stimulate oral mucosal wound healing.

scholarly journals Evaluation of in vivo wound healing activity of Moroccan Citrus reticulata peel extract

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Sanae Dahmani ◽  
Rachida Chabir ◽  
Faouzi Errachidi ◽  
Wiam Berrada ◽  
Hafsa Lansari ◽  
...  
Keyword(s):  
Wound Healing ◽  
Biological Activities ◽  
Negative Control ◽  
Area Measurement ◽  
Citrus Reticulata ◽  
Biochemical Screening ◽  
Wound Area ◽  
Peel Extract ◽  
Wound Healing Activity

Abstract Background Citrus reticulata is one of the most cultivated fruit with great benefits for humans in the world. Citrus reticulata peel has several biological activities within them hypoglycemic, hepatoprotective, antimicrobial and antioxidant. The present study emphasizes on the in vivo wound healing and in vitro antimicrobial and antioxidant activities of Citrus reticulata peel extract. Methods Forty albino mice (23–29 g) of either sex were divided into four groups. The test groups were treated with experimental ointment (0.5% and 10% of methanolic extract), negative control were treated with Vaseline and the positive control were treated with silver sulfadiazine. Burn wounds were induced on dorsal area of mice bodies. Wound area measurement was carried out every day during 22 days. Biochemical screening was performed to identify possible compounds. Antioxidant and antimicrobial activities were also determined. Results Significant wound healing activity was observed with topical application of Citrus reticulata peel extract. Wound area reduction at day 16 of treatment was 100% for both treated groups (0.5% and 10%) when compared to positive and negative control it was 100% and 98.32% respectively at day 22. Furthermore higher rate of wound contraction (100% on 16th day) was observed for both treated groups. The result of biochemical screening showed that C. reticulata peel is characterized by highest amount of total polyphenols (13.19 mg/g), flavonoids (4.07 mg/g), vitamin C (13.20 mg/g), carotenoids (0.032 mg/g) and lowest content of macronutrients (Proteins: 0.40%, reducing sugars: 7.21%; lipids: 1.5%). Additionally C. reticulata peel exhibited remarkable antioxidant activity using DPPH and phosphomolybdate methods as well as the extract possess antimicrobial effect against pathogen bactria. Conclusion The findings from this research indicated that Citrus reticulata peel extract is effective in inhibiting the growth of pathogen bacteria and could be of therapeutic potentials for wound healing.

Isolation of a stimulatory system in an Aloe extract

1991 ◽  
Vol 81 (9) ◽  
pp. 473-478 ◽  
Author(s):  
RH Davis ◽  
WL Parker ◽  
RT Samson ◽  
DP Murdoch
Keyword(s):  
Wound Healing ◽  
Lower Extremity ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Supernatant Fraction ◽  
Croton Oil ◽  
Precipitate Fraction ◽  
Anti Inflammatory ◽  
Wound Healing Activity

The authors' previous work on a 50% ethanol extract of Aloe vera was done to evaluate anti-inflammatory activity using the croton oil-induced ear swelling assay. The anti-inflammatory activity was found in the supernatant fraction. The supernatant fraction decreased inflammation, when applied topically, by 29.2%, and the precipitate decreased inflammation by 12.1%. However, in the present work, the precipitate fraction decreased the wound diameter by an average of 47.1% (stimulatory system). Little or no wound healing activity was found in the supernatant. Aloe vera appears to act as a modulatory system toward wounds and inflammation and is a potentially valuable tool for managing lower extremity conditions.

scholarly journals Evaluation of the Wound-Healing Activity of Ethanolic Extract ofMorinda citrifoliaL. Leaf

2009 ◽  
Vol 6 (3) ◽  
pp. 351-356 ◽  
Author(s):  
B. Shivananda Nayak ◽  
Steve Sandiford ◽  
Anderson Maxwell
Keyword(s):  
Wound Healing ◽  
Drinking Water ◽  
Granulation Tissue ◽  
Dead Space ◽  
Ethanol Extract ◽  
Wound Contraction ◽  
Hydroxyproline Content ◽  
Tissue Weight ◽  
Therapeutic Benefits ◽  
Wound Healing Activity

Morinda citrifoliaL. (noni) is one of the most important traditional Polynesian medicinal plants. The primary indigenous use of this plant appears to be of the leaves, as a topical treatment for wound healing. The ethanol extract of noni leaves (150 mg kg−1day−1) was used to evaluate the wound-healing activity on rats, using excision and dead space wound models. Animals were randomly divided into two groups of six for each model. Test group animals in each model were treated with the ethanol extract of noni orally by mixing in drinking water and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, time until complete epithelialization, granulation tissue weight and hydoxyproline content. On day 11, the extract-treated animals exhibited 71% reduction in the wound area when compared with controls which exhibited 57%. The granulation tissue weight and hydroxyproline content in the dead space wounds were also increased significantly in noni-treated animals compared with controls (P< 0.002). Enhanced wound contraction, decreased epithelialization time, increased hydroxyproline content and histological characteristics suggest that noni leaf extract may have therapeutic benefits in wound healing.

Wound healing activity of embelin isolated from the ethanol extract of leaves of Embelia ribes Burm.

2007 ◽  
Vol 109 (3) ◽  
pp. 529-534 ◽  
Author(s):  
H.M. Kumara Swamy ◽  
V. Krishna ◽  
K. Shankarmurthy ◽  
B. Abdul Rahiman ◽  
K.L. Mankani ◽  
...  
Keyword(s):  
Wound Healing ◽  
Ethanol Extract ◽  
Embelia Ribes ◽  
Wound Healing Activity

scholarly journals Gambaran Penyembuhan Luka Tikus Diabetes Dengan Fraksi Etil Asetat Daun Binahong (Anredera cordifolia (Tenore) Steenis)

2018 ◽  
Vol 1 (3) ◽  
pp. 158-161
Author(s):  
Trie Yuni Elfasyari ◽  
Kintoko Kintoko ◽  
Nurkhasanah Nurkhasanah
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Healing Process ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Punch Biopsy ◽  
Negative Control ◽  
Diabetic Rat ◽  
Wound Healing Process ◽  
Wound Healing Activity

Proses penyembuhan luka kulit dipengaruhi oleh kondisi dibetes mellitus (DM). Ekstrak etanol daun binahong dilaporkan dapat mempercepat penyembuhan luka tikus diabetes melalui mekanisme reepitelisasi dan kolagenasi. Penelitian ini dilakukan untuk mengetahui gambaran penyembuhan luka diabetes fraksi etil asetat daun binahong (Anredera cordifolia (Ten.) Steenis) konsentrasi 10% dengan parameter jumlah sel fibroblast pada tikus yang diinduksi sterptozotocin. Metode fraksinasi yang digunakan fraksinasi bertingkat padat cair dengan pelarut etil asetat yang bersifat semipolar. Penelitian eksperimental menggunakan 20 ekor tikus Wistar jantan usia 2 bulan, berat badan antara 150-200 gram yang diinduksi streptozotocin (STZ) dosis tunggal 45 mg/kgBB i.p. Punch biopsy digunakan untuk membuat luka pada kulit tikus dengan diameter 5 mm setelah kadar glukosa darah (KGD) di atas 250 mg/dL. Hewan uji dibagi menjadi 4 kelompok, terdiri dari kontrol sehat (tanpa induksi STZ dan tanpa perlakuan), kontrol negatif (biocream), kontrol positif (madecassol), dan fraksi etil asetat daun binahong 10% (dalam biocream). Aplikasi topikal dilakukan 2 kali sehari sebanyak 25 mg selama 10 hari pada area luka dan sekitarnya. Pengamatan dilakukan secara makroskopis dan mikroskopis. Penelitian menunjukkan bahwa fraksi etil asetat daun binahong mengandung senyawa flavonoi dan tanin, serta berperan dalam meningkatkan jumlah sel fibroblast (P<0.05).   Wound healing process is affected by diabetes melitus condition. The ethanol extract of Binahong leaves has reported to have wound healing activity on diabetic rat through mechanism of re-epithelialization and collagenation. This study was conducted to evaluate the wound healing activity on diabetic rat of ethyl acetate fraction of  Binahong (Anredera cordifolia (Tenore) Steenis) leaves at concentration of 10% with parameters of fibroblast numbers in streptozozin induced rat. The fractionation method used was solid-liquid graded fractination using ethyl acetate as semipolar solvent. This experimental research used 20 male wistar rats, aged 2 months, weight 150-200 g, induced by single dose streptozotocin (STZ)45 mg/kgBB i.p. Punch biopsy was used to injured the rat skin with diameter of 5 mm after blood glucose level was more than 250 mg/dL. Test animals were divided into 4 groups, these include healthy control (without induced by STZ and treatment), negative control (biocream), positive control (madecassol), ethyl acetate fraction of binahong leaves 10% (in biocream). The cream was applied topically 2 times a day with an amount of 25 mg for 10 days on wound area and surrounding. The macroscopic and microscopic observation was performed. The results showed that ethyl acetate fraction of binahong leaves contained flavonoids and tannin, besides it plays role to increase the fibroblast numbers  (P<0.05).

scholarly journals Evaluation of the In Vitro Wound-Healing Activity of Calabrian Honeys

Antioxidants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 36 ◽  
Author(s):  
Paolo Governa ◽  
Gabriele Carullo ◽  
Marco Biagi ◽  
Vittoria Rago ◽  
Francesca Aiello
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Biological Activities ◽  
Folk Medicine ◽  
Gas Chromatography Mass Spectrometry ◽  
Skin Wounds ◽  
Therapeutic Tools ◽  
The Many ◽  
Wound Healing Activity

The healing of skin wounds and particularly chronic wounds, such as diabetic foot ulcers, is still a clinical emergency. Despite the many therapeutic tools that are available so far, none seems to be really effective and safe. In this context, we highlighted the renewed wound healing activity of honey, a viscous aromatic and sweet food, by way of in vitro wound-healing assays, using the HaCaT cell line. Specifically, we investigated five monofloral or multifloral honeys from different Calabrian provinces using them as such or extracted (by Amberlite® or n-hexane and ethyl acetate). The chemical composition of honeys was ascertained by 1H NMR spectroscopy and by the gas chromatography/mass spectrometry (GC/MS) method for volatile organic compounds (VOCs). Amongst the five tested honeys, BL1 and BL5 honeys showed the most promising healing properties. Pinocembrin, which was revealed in BL1 (multifloral) and BL5 (orange) honey samples, is a flavanol that is already known to possess interesting biological activities, including healing. This study aims to investigate how a traditional food such as honey, which is appreciated for its nutritional value and used in folk medicine, can be enhanced as an effective modern remedial to promote a multifaceted and safe healing activity for all skin wounds.

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