scholarly journals Wound Healing Activity of Ethanolic Extract Gel of Tawas Ut Tuber (Ampelocissus rubiginosa L.) in Incisional Model Wistar Rats

2018 ◽  
Vol 23 (1) ◽  
pp. 30 ◽  
Author(s):  
Khoerul Anwar ◽  
Dewita Fitri Widodo ◽  
Nurlely Nurlely ◽  
Liling Triyasmono ◽  
Sudarsono Sudarsono ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Average Percentage ◽  
Wound Length ◽  
Wound Healing Activity

Tawas ut tuber (Ampelocissus rubiginosa Lauterb.) is empirically used in wound healing by Dayak community. The present study was undertaken to assess wound healing activity of gel from ethanol extract of A. rubiginosa tuber using parameters of the closure of wound length, tensile strength, and histopathological observations. This study used twenty-five male Wistar rats divided into five groups: positive control (Bioplacenton®), negative control (placebo gel), and three of testing groups with gel extract (concentrations of 1.5%, 2.0%, and 2.5%). Wound healing activity was evaluated to 4 cm length and ± 2 mm depth incision wound model on the back skin of rats. The measurement of wound length was observed on the day of 4th, 8th, 12th, 16th, and 20th used quantitative analyze. On the day of 20th, animal was anaesthetized and the skin has been taken for tensile strength evaluation and histopathological observations. This study showed that group treated with gel extract at the concentration of 2.5% experienced higher wound healing activity with average percentage of wound closure of 99.00% ± 0.16, tensile strength 3.8541 gram/mm2, and proved with the re-epithelization, neocapillarization, and increase collagen density appeared in histopathological observations. Based on this study, gel from ethanol extract of A. rubiginosa tuber possesses wound healing activity.

scholarly journals Assessment of Nephroprotective Potential of Ethanolic Extract Premna Tomentosa (Ept) in Alcohol Toxicity in Male Albino Rats

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 3099-3103
Author(s):  
Gomathi Priyadharsini T ◽  
Kavimani M ◽  
Sathiya Narayana Murthy S ◽  
Prabhu K ◽  
Arudyuti Chowdhury
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Blood Parameters ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Histopathological Changes

The nephroprotective prospective of Premna tomentosa extract against Alcohol induced nephrotoxicity in rats was investigated in the present study. The characterization of ethanol extract of Premna tomentosa (EPT) was performed using standard phytochemical analysis. Male albino wistar rats 36 in numbers were divided into 6 groups including control, negative control, positive control and various doses of EPT Treated groups; Nephrotoxicity was induced by alcohol (1ml/100gm b.wt) in animals. Rats intoxicated with Alcohol were fed with 500, 750 mg/kg dose of EPT and Liv 52 (1ml/100gm b.wt) for 60 days. Results show that EPT (500mg/kg b.wt) had a significant effect against alcohol induced nephrotoxicity in rats than EPT (750mg/kg b.wt) in blood parameters and consonantly good histopathological changes in kidney. The deleterious histopathological alterations in kidney associated with glomerular and tubular changes in alcohol intoxicated rats was evident. This result shows Premna tomentosa may be used as supplementary drug for alcoholics.

scholarly journals Ethanolic Extract Oitment of The Graptophylum Pictum L Griff Leaves as Analgesic agent to Male White Rat

2020 ◽  
Vol 3 (1) ◽  
pp. 32
Author(s):  
Setya Enti Rikomah ◽  
Ninsi Fitri
Keyword(s):  
White Male ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Good Effect ◽  
Test Results ◽  
Male Mice ◽  
Average Percentage ◽  
One Way Anova

<p>Graptophyllum pictum L Griff Leaves in the community are used as medicinal ingredients, one of which is for analgesics. Black pudding leaves contain secondary metabolite compounds that act as suspected analgesics, namely flavonoids. The ethanolic extract of black pudding leaves has been formulated into cream and ointment preparations. The purpose of this study was to determine the effectiveness of analgesic preparations of black pudding leaves (Graptophyllum pictum L. Griff) ethanol extract in white male mice. The effectiveness of analgesics was tested using the hotplate method. The test animals used were 25 white male mice which were divided into 5 groups: negative control (F0), positive control (Ichtiol® ointment), and black pudding leaf F1 (10%), F2 (15%) ethanol extract treatment group, and F3 (20%). Observation of stretching of mice was done after the feet of mice were induced and immediately given treatment. Observation of analgesic percentage in each mouse was carried out every 5 minutes for 60 minutes. The data obtained were processed and analyzed using one-way ANOVA and Duncan test with a 95% confidence level. One-way ANOVA test results showed that there were significant differences between treatment groups (0.003 &lt;0.05). The results of the average percentage of analgesic effectiveness of formula 2 have a good effect that is equal to 77.969%. Duncan's test results showed that the group that gave the best analgesic effectiveness was formula 2 (15%) comparable to positive control.</p><p><strong>Keywords: Graptophyllum pictum,  Analgesics, ointment, Hotplate.</strong><strong></strong></p>

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus

2020 ◽  
Vol 3 (2) ◽  
pp. 36-40
Author(s):  
Viki Ayu Intan Permatasari ◽  
Mutia Hariani Nurjanah ◽  
Wimbuh Tri Widodo
Keyword(s):  
Staphylococcus Aureus ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Negative Control ◽  
Traditional Medicines ◽  
Alternative Ingredients ◽  
High Concentrations

Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations

Aktivitas Antibakteri dari Ekstrak Etanol Ganoderma sp. Asal Pulau Lombok

2020 ◽  
Vol 6 (1) ◽  
pp. 22
Author(s):  
Adelina Oktaviani ◽  
Aida Muspiah ◽  
Faturrahman Faturrahman
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Activity ◽  
Large Diameter ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Gram Negative Bacteria ◽  
Activity Test

The use of antibiotics that are not according to the rules and antibiotics in the long term can cause resistance to bacteria. This study aims to determine the presence of antibacterial activity and the effect of increasing the concentration of ethanol extract of Ganoderma sp. against several test bacteria. Extract from Ganoderma sp. obtained by maceration method using ethanol 95% solvent. The extract concentrations used were 20%, 40%, 60% and 80%. This research was conducted using the wells method with ciprofloxacin as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition formed around the well. The results of the antibacterial activity test of ethanol extract Ganoderma sp. has greater inhibitory activity against gram-negative bacteria. The inhibitory activity of ethanolic extract of Ganoderma sp. on the growth of test bacteria increased with increasing concentration of the extract

scholarly journals Antiinflammatory Activity of Pagoda Flower (Clerodendrum Paniculatum L.) Ethanol Extract Using Paw Edema Method

2019 ◽  
Vol 7 (6) ◽  
pp. 43-45
Author(s):  
Ihsanul Hafiz ◽  
Mandike Ginting
Keyword(s):  
Antiinflammatory Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Paw Edema ◽  
Laboratory Design ◽  
White Rats ◽  
Experimental Laboratory ◽  
The Difference

Objectives: The purpose of this study was to determine antiinflammatory activity of pagoda flower (Clerodendrum paniculatum L.) ethanolic extract Design: This study uses an experimental laboratory design. This research uses paw edema method by inducing carrageenin in the legs of male white rats as an induction of inflammation. Interventions: The sample used was pagoda flower ethanol extract in various dosages of 25, 50 and 100 mg / kg. As a comparison, acetosal dose 33 mg / kg was used. Na CMC suspension was used as a negative control. Main outcome measure: The results in this study are the difference in the volume of edema volume from rat feet per unit time. The measurement of the rat's leg volume was measured at 30, 60, 120, 180, 240 and 300 minutes. Conclusion: Pagoda flower ethanol extract does not have good anti-inflammatory activity. there were no significant differences between groups except at dose 100 and positive control at minute 300 of negative control.    

scholarly journals Preparation, Characterization, and Wound Healing Activity of Papaya Leaves Extract on Spray Gel

2020 ◽  
Vol 25 (2) ◽  
pp. 103
Author(s):  
Dina Permata Wijaya ◽  
Herlina Herlina ◽  
Najma Annuria Fitri ◽  
Mardiyanto Mardiyanto ◽  
Mustikasanti Mustikasanti ◽  
...  
Keyword(s):  
Wound Healing ◽  
Positive Control ◽  
Negative Control ◽  
Gellan Gum ◽  
Gelling Agent ◽  
Carbopol 940 ◽  
Ph Range ◽  
Wound Healing Activity

Papaya leaves have been using for wound healing that contains flavonoids, saponins, phenolics, chymopapain, and papain enzymes. The aim of this research were preparation, characterization, and wound healing activity of papaya leaves extract on spray gel. Spray gel was formulated with variation of gelling agent such as carbopol 940, HPMC, gellan gum, and hydroxyethylcellulose. The spray gel were characterizated by organoleptic, pH, stickiness test, viscosity, homogeneity, weight, and wound healing activity in rats. The results showed were all of formula spray gel have brown and homogeneous, pH between 5,947-6,347 within pH range of skin, stickiness test between 1,92-8,12 s, viscosity between 880-1740 cPs. Papaya leaves extract on spray gel has wound healing activity in rats faster than extract and positive control that is 16 days. The wound healing of papaya leaves extract on spray gel exhibited significantly different (p<0,05) than negative control.

scholarly journals Burn Wound Healing Activity of Ethanolic Extract of Acalypha indica in Oinment Formulated against Rabbits (Ocyctagus caniculus)

2020 ◽  
Vol 8 (3) ◽  
pp. 18-20
Author(s):  
Khairani Fitri ◽  
Tetty Noverita Khairani ◽  
Fajar Apollo Sinaga ◽  
Amenia Gracia Talunohi
Keyword(s):  
Wound Healing ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Burn Wound ◽  
Leaf Extracts ◽  
Laboratory Design ◽  
Burn Wound Healing ◽  
Healing Phase ◽  
Wound Healing Activity ◽  
Acalypha Indica

Objectives: The purpose of this study was to determine burn wound healing activity of Acalypha indica L. in oinment formulated. Design: This study uses an experimental laboratory design. This study used rabbits as test animals induced by burns with hot iron plates on the back. Extract ointment applied to the wound then observed the development of healing. Interventions: The extract was formulated in ointments with concentrations of 3, 5 and 7%. positive control used was ointment containing sesame oil and negative control used is an ointment base. Main outcome measure: The results showed a concentration of 3% had a slow and low effect, at a concentration of 5% it had a moderate healing phase, and a concentration of 7% had a good healing phase. Conclusion: The ointments containing Acalypha indica leaf extracts have good activity in healing burns in rabbits.    

scholarly journals Evaluation of Wound Healing Activity of an Ethanolic Extract of Anacardiumoccidentale Leaves in Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 2061-2068
Author(s):  
Meena Kumari K ◽  
Amberkar Mohanbabu Vittalrao ◽  
Praveen Kumar SE ◽  
Sushma Prabhath ◽  
Charitha Charitha
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Saline Control ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Healing Activity

BACKGROUND -This study undertaken to screen a potential wound healing activity of an ethanolic extract of Anacardiumoccidentale(Ao)leaves in Wistar rats. MATERIALS AND METHODS-Excision wound of 500mm2created on the back of Wistar rats. A total of thirty adult Wistar rats were prescreened and divided into five groups of six rats each.Group I and II rats were treated by normal saline (control) and povidone-iodine (standard drug), respectively. Group III and IV with 0.5% and 2%of Anacardiumoccidentale ointment (test drugs), respectively, and Group V rats treated with anti-wound healer drug dexamethasone, i.m. + 2% Anacardiumoccidentale. All drugs were applied topically except Inj. dexamethasone. In the model of excision wound, the wound healing effect was determined by parameters such as the percentage of wound contraction, duration of epithelialization, and histopathological examination. RESULT-All drugs, povidone-iodine, 0.5%, and 2% of the Ao ointment treated group of rats showed a statistically significant increase (P<0.05) in the % of wound contraction on 5th and 6thdays Vs.Control rats. In contrast,povidone-iodine, 2% Ao ointment and (dexamethasone + 2% Ao)treated rats decreased the period of epithelialization which was statistically significant (P<0.05) compared to the control.However, the standard povidone-iodine treated rats showed a maximum increase in the percentage of wound contraction and minimum period of epithelialization, followed by a higher dose of 2% Ao extract. The plant extract 0.5% and 2% of Ao showeda significant increase inthe rate of wound contraction as compared to the standard drug povidone-iodine also. The combination of (2%Aoextract+dexamethasone) drug-treated rats decreased the rate of wound contraction on 5th, 9th days; whereas, povidone-iodine increased on all days, i.e., 5th, 9th, 13th and 17th days,which was statistically significant (p<0.05) Vs.Normal saline. The povidone and 2% of Ao extract hastened the rate of wound healing activity, which was evident by maximum re-epithelialization and presence of the collagen fibers. CONCLUSION-In our study, the topical application of Anacardiumoccidentale leaf extract has shown promising wound healing activity; however, it failed to reverse the anti-wound healing property of steroids.

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